Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,244,390 T→C 8.2% L141P (CTG→CCG)  PP_1980 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,244,3900TC 8.2% 169.1 / 5.2 61L141P (CTG→CCG) PP_1980hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (29/27);  new base C (2/3);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.02e-01

CTGCGCATGACGCGGCGGTTTCAGCTCAAGCGCCCGCGCGATGGTGTGACGCTGCTGCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATCCTGCGCGACGTATTGACTCGTGTGGGCGGTATC  >  NC_002947/2244242‑2244532
                                                                                                                                                    |                                                                                                                                              
cTGCGCATGACGCGGCGGTTTCAGCTCAAGCGCCCGCGCGATGGTGTGACGCTGCTGCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCt                                                                                                                                                <  3:400390/149‑1 (MQ=255)
       tGACGCGGCGGTTTCAGCTCAAGCGCCCGCGCGATGGTGTGACGCTGCTGCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGgc                                                                                                                                         >  5:35464/1‑149 (MQ=255)
            cggcggTTTCAGCTCAAGCGCCCGCGCGATGGTGTGACGCTGCTGCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGTCGGAATTCGTCGAAGGTTACGGTCCGGCGCCGATCAGCGccgg                                                                                                                                    <  5:362329/149‑3 (MQ=255)
              gcggTTTCAGCTCAAGCGCCCGCGCGATGGTGTGACGCTGCTGCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAg                                                                                                                                  <  2:323596/149‑1 (MQ=255)
               cggTTTCAGCTCAAGCGCCCGCGCGATGGTGTGACGCTGCTGCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGt                                                                                                                                 <  8:9892/149‑1 (MQ=255)
                 gTTTCAGCTCAAGCGCCCGCGCGATGGTGTGACGCTGCTGCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTgg                                                                                                                               <  8:389802/149‑1 (MQ=255)
                        cTCAAGCGCCCGCGCGATGGTGTGACGCTGCTGCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCAt                                                                                                                        >  5:320990/1‑149 (MQ=255)
                        cTCAAGCGCCCGCGCGATGGTGTGACGCTGCTGCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCAt                                                                                                                        >  7:57246/1‑149 (MQ=255)
                         tCAAGCGCCCGCGCGATGGTGTGACGCTGCTGCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCAtt                                                                                                                       >  8:342629/1‑149 (MQ=255)
                         tCAAGCGCCCGCGCGATGGTGTGACGCTGCTGCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCAtt                                                                                                                       >  8:227111/1‑149 (MQ=255)
                              cgcCCGCGCGATGGTGTGACGCTGCTGCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTg                                                                                                                  <  6:320990/149‑1 (MQ=255)
                               gcCCGCGCGATGGTGTGACGCTGCTGCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGt                                                                                                                 >  7:329397/1‑149 (MQ=255)
                                cccGCGCGATGGTGTGACGCTGCTGCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGt                                                                                                                 >  8:155476/1‑148 (MQ=255)
                                   gcgcgATGGTGTGACGCTGCTGCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTaaa                                                                                                             <  6:228861/149‑1 (MQ=255)
                                        aTGGTGTGACGCTGCTGCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGcc                                                                                                        <  6:35464/149‑1 (MQ=255)
                                                aCGCTGCTGCGTGCGCAAAGCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCtttt                                                                                                 <  8:320402/148‑1 (MQ=255)
                                                   ctgctgCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCg                                                                                             <  5:168908/149‑1 (MQ=255)
                                                         cgtgcgCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTgaga                                                                                       >  4:7888/1‑149 (MQ=255)
                                                             cgcAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCCGATCAGCGCCGGa                                                                                                                                   <  3:46344/101‑1 (MQ=255)
                                                             cgcAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCCGATCAGCGCCGGa                                                                                                                                   >  4:291969/1‑101 (MQ=255)
                                                             cgcAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCCGATCAGCGCCGGa                                                                                                                                   <  3:291969/101‑1 (MQ=255)
                                                             cgcAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCCGATCAGCGCCGGa                                                                                                                                   >  4:46344/1‑101 (MQ=255)
                                                                 aaCCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACa                                                                               <  8:57246/149‑1 (MQ=255)
                                                                          cGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAAtt                                                                       <  7:342629/148‑1 (MQ=255)
                                                                          cGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAAtt                                                                       <  3:321418/148‑1 (MQ=255)
                                                                            ccTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGcc                                                                    <  7:12692/149‑1 (MQ=255)
                                                                            ccTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGcc                                                                    >  3:389435/1‑149 (MQ=255)
                                                                             cTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGccc                                                                   >  4:274038/1‑149 (MQ=255)
                                                                              tGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCt                                                                  >  7:331610/1‑149 (MQ=255)
                                                                                 aTCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGc                                                               >  7:70865/1‑149 (MQ=255)
                                                                                         gTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTc                                                                                               <  4:91917/109‑1 (MQ=255)
                                                                                         gTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTc                                                                                               >  3:91917/1‑109 (MQ=255)
                                                                                          tCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATg                                                        >  6:403793/1‑147 (MQ=255)
                                                                                          tCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATg                                                        <  3:274038/147‑1 (MQ=255)
                                                                                                  cAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTgg                                              <  5:403793/149‑1 (MQ=255)
                                                                                                      cccAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGTCCCCGACATGCAAATTGCCCTGGCCCCCATGGAgggg                                                  >  7:16512/1‑141 (MQ=255)
                                                                                                      cccAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGTCCCCGACATGCAAATTGCCCTGGCCCCCATGGAgggg                                                  <  8:16512/141‑1 (MQ=255)
                                                                                                      cccAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGa                                          >  7:106447/1‑149 (MQ=255)
                                                                                                       ccAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGa                                          >  6:212228/1‑148 (MQ=255)
                                                                                                          ggCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGacaac                                      <  2:240486/149‑1 (MQ=255)
                                                                                                          ggCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGacaac                                      <  3:7888/149‑1 (MQ=255)
                                                                                                             gcATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATc                                   <  4:389435/149‑1 (MQ=255)
                                                                                                                 gCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATCCTgc                               >  1:124148/1‑149 (MQ=255)
                                                                                                                    ggcggAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATCCTGcgcg                            <  8:329397/149‑1 (MQ=255)
                                                                                                                     gcggAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATCCTGCGCGa                           >  3:200951/1‑149 (MQ=255)
                                                                                                                      cggAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATCCTGCGCGAc                          <  7:155476/149‑1 (MQ=255)
                                                                                                                              gtcgAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGc                                                                                                         >  1:374255/1‑62 (MQ=255)
                                                                                                                              gtcgAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGc                                                                                                         <  2:374255/62‑1 (MQ=255)
                                                                                                                              gtcgAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATCCTGCGCGACGTAt                      <  6:269736/145‑1 (MQ=255)
                                                                                                                              gtcgAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATCCTGCGCGACGTAt                      >  5:269736/1‑145 (MQ=255)
                                                                                                                               tcgAAGGTTACGGTCCGGCGCTGATCTGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGACGGCTTTTTCAGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGTGCTGGTCGACAACATCCTGCGCTACGTATTGACt                 >  1:410798/1‑149 (MQ=255)
                                                                                                                               tcgAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCAt                                                         >  5:280905/1‑109 (MQ=255)
                                                                                                                               tcgAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCAt                                                         <  6:280905/109‑1 (MQ=255)
                                                                                                                               tcgAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAgg                                                    <  2:129826/114‑1 (MQ=255)
                                                                                                                               tcgAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAgg                                                    >  1:129826/1‑114 (MQ=255)
                                                                                                                                  aaGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGcc                                                                    >  6:50478/1‑95 (MQ=255)
                                                                                                                                     gTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATCCTGCGCGACGTATTGACTCGtgtg           <  5:212228/149‑1 (MQ=255)
                                                                                                                                            tCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATCCTGCGCGACGTATTGACTCGTGTGGGc        <  7:96424/145‑1 (MQ=255)
                                                                                                                                            tCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATCCTGCGCGACGTATTGACTCGTGTGGGc        >  8:96424/1‑145 (MQ=255)
                                                                                                                                            tCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATCCTGCGCGACGTATTGACTCGTGTGGGCGGTa    >  7:232957/1‑149 (MQ=255)
                                                                                                                                            tCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATCCTGCGCGACGTATTGACTCGTGTGGGCGGTa    >  1:240409/1‑149 (MQ=255)
                                                                                                                                               ggCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATCCTGCGCGACGTATTGACTCGTGTGGGCGGTATc  >  7:330560/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
CTGCGCATGACGCGGCGGTTTCAGCTCAAGCGCCCGCGCGATGGTGTGACGCTGCTGCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATCCTGCGCGACGTATTGACTCGTGTGGGCGGTATC  >  NC_002947/2244242‑2244532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑