Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,244,395 G→A 8.0% G143S (GGC→AGC)  PP_1980 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,244,3950GA 8.0% 182.1 / 4.1 63G143S (GGC→AGC) PP_1980hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (28/29);  new base A (2/3);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.41e-01

TGACGCGGCGGTTTCAGCTCAAGCGCCCGCGCGATGGTGTGACGCTGCTGCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATCCTGCGCGACGTATTGACTCGTGTGGGCGGTATCGACTGGTGC  >  NC_002947/2244249‑2244541
                                                                                                                                                  |                                                                                                                                                  
tGACGCGGCGGTTTCAGCTCAAGCGCCCGCGCGATGGTGTGACGCTGCTGCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGgc                                                                                                                                                  >  5:35464/1‑149 (MQ=255)
     cggcggTTTCAGCTCAAGCGCCCGCGCGATGGTGTGACGCTGCTGCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGTCGGAATTCGTCGAAGGTTACGGTCCGGCGCCGATCAGCGccgg                                                                                                                                             <  5:362329/149‑3 (MQ=255)
       gcggTTTCAGCTCAAGCGCCCGCGCGATGGTGTGACGCTGCTGCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAg                                                                                                                                           <  2:323596/149‑1 (MQ=255)
        cggTTTCAGCTCAAGCGCCCGCGCGATGGTGTGACGCTGCTGCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGt                                                                                                                                          <  8:9892/149‑1 (MQ=255)
          gTTTCAGCTCAAGCGCCCGCGCGATGGTGTGACGCTGCTGCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTgg                                                                                                                                        <  8:389802/149‑1 (MQ=255)
                 cTCAAGCGCCCGCGCGATGGTGTGACGCTGCTGCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCAt                                                                                                                                 >  7:57246/1‑149 (MQ=255)
                 cTCAAGCGCCCGCGCGATGGTGTGACGCTGCTGCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCAt                                                                                                                                 >  5:320990/1‑149 (MQ=255)
                  tCAAGCGCCCGCGCGATGGTGTGACGCTGCTGCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCAtt                                                                                                                                >  8:342629/1‑149 (MQ=255)
                  tCAAGCGCCCGCGCGATGGTGTGACGCTGCTGCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCAtt                                                                                                                                >  8:227111/1‑149 (MQ=255)
                       cgcCCGCGCGATGGTGTGACGCTGCTGCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTg                                                                                                                           <  6:320990/149‑1 (MQ=255)
                        gcCCGCGCGATGGTGTGACGCTGCTGCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGt                                                                                                                          >  7:329397/1‑149 (MQ=255)
                         cccGCGCGATGGTGTGACGCTGCTGCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGt                                                                                                                          >  8:155476/1‑148 (MQ=255)
                            gcgcgATGGTGTGACGCTGCTGCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTaaa                                                                                                                      <  6:228861/149‑1 (MQ=255)
                                 aTGGTGTGACGCTGCTGCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGcc                                                                                                                 <  6:35464/149‑1 (MQ=255)
                                         aCGCTGCTGCGTGCGCAAAGCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCtttt                                                                                                          <  8:320402/148‑1 (MQ=255)
                                            ctgctgCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCg                                                                                                      <  5:168908/149‑1 (MQ=255)
                                                  cgtgcgCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTgaga                                                                                                >  4:7888/1‑149 (MQ=255)
                                                      cgcAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCCGATCAGCGCCGGa                                                                                                                                            <  3:46344/101‑1 (MQ=255)
                                                      cgcAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCCGATCAGCGCCGGa                                                                                                                                            >  4:291969/1‑101 (MQ=255)
                                                      cgcAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCCGATCAGCGCCGGa                                                                                                                                            >  4:46344/1‑101 (MQ=255)
                                                      cgcAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCCGATCAGCGCCGGa                                                                                                                                            <  3:291969/101‑1 (MQ=255)
                                                          aaCCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACa                                                                                        <  8:57246/149‑1 (MQ=255)
                                                                   cGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAAtt                                                                                <  7:342629/148‑1 (MQ=255)
                                                                   cGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAAtt                                                                                <  3:321418/148‑1 (MQ=255)
                                                                     ccTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGcc                                                                             >  3:389435/1‑149 (MQ=255)
                                                                     ccTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGcc                                                                             <  7:12692/149‑1 (MQ=255)
                                                                      cTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGccc                                                                            >  4:274038/1‑149 (MQ=255)
                                                                       tGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCt                                                                           >  7:331610/1‑149 (MQ=255)
                                                                          aTCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGc                                                                        >  7:70865/1‑149 (MQ=255)
                                                                                  gTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTc                                                                                                        <  4:91917/109‑1 (MQ=255)
                                                                                  gTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTc                                                                                                        >  3:91917/1‑109 (MQ=255)
                                                                                   tCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATg                                                                 <  3:274038/147‑1 (MQ=255)
                                                                                   tCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATg                                                                 >  6:403793/1‑147 (MQ=255)
                                                                                           cAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTgg                                                       <  5:403793/149‑1 (MQ=255)
                                                                                               cccAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGTCCCCGACATGCAAATTGCCCTGGCCCCCATGGAgggg                                                           <  8:16512/141‑1 (MQ=255)
                                                                                               cccAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGTCCCCGACATGCAAATTGCCCTGGCCCCCATGGAgggg                                                           >  7:16512/1‑141 (MQ=255)
                                                                                               cccAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGa                                                   >  7:106447/1‑149 (MQ=255)
                                                                                                ccAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGa                                                   >  6:212228/1‑148 (MQ=255)
                                                                                                   ggCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGacaac                                               <  3:7888/149‑1 (MQ=255)
                                                                                                   ggCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGacaac                                               <  2:240486/149‑1 (MQ=255)
                                                                                                      gcATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATc                                            <  4:389435/149‑1 (MQ=255)
                                                                                                          gCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATCCTgc                                        >  1:124148/1‑149 (MQ=255)
                                                                                                             ggcggAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATCCTGcgcg                                     <  8:329397/149‑1 (MQ=255)
                                                                                                              gcggAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATCCTGCGCGa                                    >  3:200951/1‑149 (MQ=255)
                                                                                                               cggAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATCCTGCGCGAc                                   <  7:155476/149‑1 (MQ=255)
                                                                                                                       gtcgAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGc                                                                                                                  <  2:374255/62‑1 (MQ=255)
                                                                                                                       gtcgAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGc                                                                                                                  >  1:374255/1‑62 (MQ=255)
                                                                                                                       gtcgAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATCCTGCGCGACGTAt                               >  5:269736/1‑145 (MQ=255)
                                                                                                                       gtcgAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATCCTGCGCGACGTAt                               <  6:269736/145‑1 (MQ=255)
                                                                                                                        tcgAAGGTTACGGTCCGGCGCTGATCTGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGACGGCTTTTTCAGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGTGCTGGTCGACAACATCCTGCGCTACGTATTGACt                          >  1:410798/1‑149 (MQ=255)
                                                                                                                        tcgAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCAt                                                                  <  6:280905/109‑1 (MQ=255)
                                                                                                                        tcgAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCAt                                                                  >  5:280905/1‑109 (MQ=255)
                                                                                                                        tcgAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAgg                                                             <  2:129826/114‑1 (MQ=255)
                                                                                                                        tcgAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAgg                                                             >  1:129826/1‑114 (MQ=255)
                                                                                                                           aaGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGcc                                                                             >  6:50478/1‑95 (MQ=255)
                                                                                                                              gTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATCCTGCGCGACGTATTGACTCGtgtg                    <  5:212228/149‑1 (MQ=255)
                                                                                                                                     tCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATCCTGCGCGACGTATTGACTCGTGTGGGc                 <  7:96424/145‑1 (MQ=255)
                                                                                                                                     tCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATCCTGCGCGACGTATTGACTCGTGTGGGc                 >  8:96424/1‑145 (MQ=255)
                                                                                                                                     tCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATCCTGCGCGACGTATTGACTCGTGTGGGCGGTa             >  7:232957/1‑149 (MQ=255)
                                                                                                                                     tCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATCCTGCGCGACGTATTGACTCGTGTGGGCGGTa             >  1:240409/1‑149 (MQ=255)
                                                                                                                                        ggCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATCCTGCGCGACGTATTGACTCGTGTGGGCGGTATc           >  7:330560/1‑148 (MQ=255)
                                                                                                                                             tgttCGGCGCGTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATCCTGCGCGACGTATTGACTCGTGTGGGCGGTATCGACTgg     <  2:410798/146‑1 (MQ=255)
                                                                                                                                                tCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATCCTGCGCGACGTATTGACTCGTGTGGGCGGTATCGACTGGTGc  <  8:232957/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TGACGCGGCGGTTTCAGCTCAAGCGCCCGCGCGATGGTGTGACGCTGCTGCGTGCGCAAACCACCTTCGCCTGCATCGAGCTGTCCAGCGGCAAGCCCAGGCGCATGCCGGCGGAATTCGTCGAAGGTTACGGTCCGGCGCTGATCGGCGCCTGAGTGGCCGGCATTTTTTGTTAAACTGCCGGCTTTTTCCGCTGAGACCCCGACATGCAAATTGCCCTGGCCCCCATGGAGGGGCTGGTCGACAACATCCTGCGCGACGTATTGACTCGTGTGGGCGGTATCGACTGGTGC  >  NC_002947/2244249‑2244541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑