| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,252,647 | T→A | 8.2% | L183Q (CTG→CAG) | leuD → | 3‑isopropylmalate dehydratase small subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,252,647 | 0 | T | A | 8.2% | 252.6 / 9.4 | 85 | L183Q (CTG→CAG) | leuD | 3‑isopropylmalate dehydratase small subunit |
| Reads supporting (aligned to +/- strand): ref base T (40/38); new base A (3/4); total (43/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01 | |||||||||||
AAGTCGACGAGCTGTTCAAGCAGGTCGAAGCCAACCCGGGCTACCAGCTGACCATCGACCTGCAGGCGCAGGCGGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGC > NC_002947/2252500‑2252793 | aaGTCGACGAGCTGTTCAAGCAGGTCGAAGCCAACCCGGGCTACCAGCTGACCATCGACCTGCAGGCGCAGGCGGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTg < 5:233526/149‑1 (MQ=255) gTCGACGAGCTGTTCAAGCAGGTCGAAGCCAACCCGGGCTACCAGCTGACCATCGACCTGCAGGCGCAGGCGGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGa < 1:40469/149‑1 (MQ=255) gacgaGCTGTTCAAGCAGGTCGAAGCCAACCCGGGCTACCAGCTGACAATCGACCTGCAGGCGCAGGCGGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGa > 5:191292/1‑149 (MQ=255) gacgaGCTGTTCAAGCAGGTCGAAGCCAACACGGGCTACCAGCTGACCATCGACCTGCAGGCGCAGGCGGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGa > 1:243665/1‑149 (MQ=255) gTTCAAGCAGGTCGAAGCCAACCCGGGCTACCAGCTGACCATCGACCTGCAGGCGCAGGCGGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCAGGCCGATATCgtc > 3:302304/1‑146 (MQ=255) aaGCAGGTCGAAGCCAACCCGGGCTACCAGCTGACCATCGACCTGCAGGCGCAGGCGGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCAGGCCGATATCGTCCAG‑Ac < 4:302304/149‑1 (MQ=255) aGCAGGTCGAAGCCAACCCGGGCTACCAGCTGACCATCGACCTGCAGGCGCAGGCGGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGACA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCAGGCCGATATCGTCCAG‑Acc < 5:219788/149‑1 (MQ=255) aGGTCGAAGCCAACCCGGGCTACCAGCTGACCATCGACCTGCAGGCGCAGGCGGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCAGGCCGATATCGTCCAG‑Accgtt > 1:380010/1‑146 (MQ=255) aGGTCGAAGCCAACCCGGGCTACCAGCTGACCATCGACCTGCAGGCGCAGGCGGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCAGGCCGATATCGTCCAG‑Accgtt < 2:380010/149‑4 (MQ=255) ggTCGAAGCCAACCCGGGCTACCAGCTGACCATCGACCTGCAGGCGCAGGCGGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGc < 4:215328/149‑1 (MQ=255) gTCGAAGCCAACCCGGGCTACCAGCTGACCATCGACCTGCAGGCGCAGGCGGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCa < 5:48074/149‑1 (MQ=255) ctCGAAGCCAAACCGGGGTACCACCTGACCATAGACCTGCAGGCGCAGCCGCTGACCCCGCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCCCAAGCACTGCCAGCTCACCGGTCTGGACGATATCGGCCGG‑ACGTTg > 4:201386/2‑147 (MQ=255) tggAATCCAACCCGGGGTACCAGCTGTACATCGCACTTCAGGCGGCCGGGGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAg < 6:296985/147‑1 (MQ=255) tCGAAGCCAACCCGGGCTACCAGCTGACCATCGACCTGCAGGCGCAGGCGGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAg > 4:150263/1‑149 (MQ=255) gAAGCCAACCCGGGCTACCAGCTGACCATCGACCTGCAGGCGCAGGCGGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGa > 4:186639/1‑149 (MQ=255) cAACCCGGGCTACCAGCTGACCATCGACCTGCAGGCGCAGGCGGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGc > 2:15193/1‑148 (MQ=255) aaCCCGGGCTACCAGCTGACCATCGACCTGCAGGCGCAGGCGGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGa > 4:243088/1‑149 (MQ=255) aaCCCGGGCTACCAGCTGACCATCGACCTGCAGGCGCAGGCGGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGa > 3:388382/1‑149 (MQ=255) ccGGGCTACCAGCTGACCATCGACCTGCAGGCGCAGGCGGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGAcgc < 2:243665/149‑1 (MQ=255) gggCTACCAGCTGACCATCGAGCTGCTGGCGCAGGCGGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGACA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGGCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACTCGa < 3:201386/149‑1 (MQ=255) gggCTACCAGCTGACCATCGACCTGCAGGCGCAGGCGGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGa < 2:57317/149‑1 (MQ=255) aGCTGACCATCGACCTGCAGGCGCAGGCGGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGcc > 5:23055/1‑149 (MQ=255) cTGACCATCGACCTGCAGGCGCAGGCGGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCtt < 3:186639/149‑1 (MQ=255) gACCATCGACCTGCAGGCGCAGGCGGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCa > 4:234800/1‑123 (MQ=255) gACCATCGACCTGCAGGCGCAGGCGGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCa < 3:234800/123‑1 (MQ=255) ccATCGACCTGCAGGCGCAGGCGGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGaa < 8:138441/149‑1 (MQ=255) aTCGACCTGCAGGCGCAGGCGGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGAAGCGATCAAGGCCTTCGAAg > 5:106210/1‑148 (MQ=255) gACCTGCAGGCGCAGGCGGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCaa < 3:301034/149‑1 (MQ=255) ccTGCAGGCGCAGGCGGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGc < 1:15193/149‑1 (MQ=255) cTGCAGGCGCAGGCGGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑Acc < 8:33128/108‑1 (MQ=255) cTGCAGGCGCAGGCGGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑Acc > 7:33128/1‑108 (MQ=255) cTGCAGGCGCAGGCGGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGTAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCa > 6:397794/1‑149 (MQ=255) cTGCAGGCGCAGGCGGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCAGGCCGATATCGTCCAG‑Acc < 1:80393/108‑1 (MQ=255) cTGCAGGCGCAGGCGGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCAGGCCGATATCGTCCAG‑Acc > 2:80393/1‑108 (MQ=255) cgcaggcgGTGACCCGCCCCGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCAGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCgcgc > 5:228528/1‑148 (MQ=255) gcaggcgGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGAGATTCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCgcgc > 1:291780/1‑148 (MQ=255) gcaggcgGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCgcca < 4:388382/149‑1 (MQ=255) gcgGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCgccagc > 4:393613/1‑147 (MQ=255) gcgGTGACACGCCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCgccagc > 2:243362/1‑147 (MQ=255) cccgcccgGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAgg > 3:13406/1‑114 (MQ=255) cccgcccgGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAgg < 4:13406/114‑1 (MQ=255) cccgcccgGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGcc < 6:85959/125‑1 (MQ=255) cccgcccgGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGcc > 5:85959/1‑125 (MQ=255) cccgcccgGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTgg < 2:124920/149‑1 (MQ=255) ccgcccgGATGGCAATGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCTATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGc < 6:228528/149‑1 (MQ=255) ccgcccgGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCgccagcc > 4:23313/1‑140 (MQ=255) ccgcccgGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCgccagcc < 3:23313/140‑1 (MQ=255) ccgcccgGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGc > 6:19795/1‑149 (MQ=255) ccgcccgGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGc > 2:348179/1‑149 (MQ=255) gcccgGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTgg < 4:77675/146‑1 (MQ=255) gcccgGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTgg > 3:77675/1‑146 (MQ=255) cgGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACcgc < 7:347801/128‑1 (MQ=255) cgGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACcgc > 8:347801/1‑128 (MQ=255) aTGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGt < 3:150263/149‑1 (MQ=255) ggCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGa > 3:214647/1‑149 (MQ=255) cAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCgcc < 2:20343/124‑1 (MQ=255) cAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCgcc > 1:20343/1‑124 (MQ=255) cAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCtt > 7:240486/1‑134 (MQ=255) cAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCtt < 8:240486/134‑1 (MQ=255) ctgcACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGaa < 1:243362/148‑1 (MQ=255) ctgcACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGaa < 1:348179/148‑1 (MQ=255) gcACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAgg < 6:245696/92‑1 (MQ=255) gcACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAgg > 5:245696/1‑92 (MQ=255) cACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGTAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTc < 5:397794/149‑1 (MQ=255) ttCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCt > 2:5949/1‑120 (MQ=255) ttCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCt < 1:5949/120‑1 (MQ=255) cGAGT‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTa < 2:291780/147‑1 (MQ=255) cGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTa < 6:191292/147‑1 (MQ=255) ga‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAg < 6:131821/145‑1 (MQ=255) ga‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAg > 5:131821/1‑145 (MQ=255) cGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAg > 6:388329/1‑149 (MQ=255) gTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTGAACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCgg > 1:90820/1‑149 (MQ=255) gTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCggg > 7:388133/1‑149 (MQ=255) ccGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGcct > 6:152448/1‑149 (MQ=255) aCTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTc < 2:43061/149‑1 (MQ=255) gcTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAg > 2:64156/1‑148 (MQ=255) cTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGa > 5:7253/1‑148 (MQ=255) tCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGa > 1:160861/1‑147 (MQ=255) cAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAg > 5:376736/1‑149 (MQ=255) aaCGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGa > 6:257177/1‑118 (MQ=255) aaCGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGa < 5:257177/118‑1 (MQ=255) aaCGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGaaa < 4:214647/147‑1 (MQ=255) aCGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGtt < 6:106210/149‑1 (MQ=255) gTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTAcg > 1:51989/1‑149 (MQ=255) tCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTAcgc < 2:90820/149‑1 (MQ=255) | AAGTCGACGAGCTGTTCAAGCAGGTCGAAGCCAACCCGGGCTACCAGCTGACCATCGACCTGCAGGCGCAGGCGGTGACCCGCCCGGATGGCAAGGTGCTGCACTTCGAGA‑TCGATGCGTTCCGCAAGCACTGCCTGCTCAACGGTCTGGACGATATCGGCCTG‑ACCTTGCAGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGC > NC_002947/2252500‑2252793 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |