Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,260,746 G→C 6.4% G445A (GGC→GCC)  fimV → motility protein FimV

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,260,7460GC 6.4% 202.4 / 4.4 63G445A (GGC→GCC) fimVmotility protein FimV
Reads supporting (aligned to +/- strand):  ref base G (28/30);  new base C (2/2);  total (30/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.86e-01

CAGCATCCTGGTGCTGGCACTGCTGCTGTGGCTGCTGGCGCGCAAACGCAAGGCCCAGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTGGCAGAACCGGTCGCCGAGGCAGCACCCGAGCCTGAACCACAAGCAGACCCGCACGCCGCGCTG  >  NC_002947/2260600‑2260894
                                                                                                                                                  |                                                                                                                                                    
cAGCATCCTGGTGCTGGCACTGCTGCTGTGGCTGCTGGCGCGCAAACGCAAGGCCCAGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCggcggc                                                                                                                                                     <  4:49323/148‑1 (MQ=255)
      ccTGGTGCTGGCACTGCTGCTGTGGCTGCTGGCGCGCAAACGCAAGGCCCAGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAg                                                                                                                                              >  3:268862/1‑149 (MQ=255)
                gCACTGCTGCTGTGGCTGCTGGCGCGCAAACGCAAGGCCCAGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGcc                                                                                                                                    >  7:138134/1‑149 (MQ=255)
                gCACTGCTGCTGTGGCTGCTGGCGCGCAAACGCAAGGCCCAGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGACGCCGCCGAAGCTCTCGat                                                                                                                                      <  3:186331/147‑2 (MQ=255)
                  aCTGCTGCTGTGGCTGCTGGCGCGCAAACGCAAGGCCCAGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCgg                                                                                                                                  <  2:117066/149‑1 (MQ=255)
                    tgctgctgTGGCTGCTGGCGCGCAAACGCAAGGCCCAGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCCAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGgt                                                                                                                                >  3:144056/1‑149 (MQ=255)
                      ctgctgTGGCTGCTGGCGCGCAAACGCAAGGCCCAGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGgt                                                                                                                                <  1:11618/147‑1 (MQ=255)
                      ctgctgTGGCTGCTGGCGCGCAAACGCAAGGCCCAGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGgt                                                                                                                                <  2:390477/147‑1 (MQ=255)
                         ctgTGGCTGCTGGCGCGCAAACGCAAGGCCCAGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAAc                                                                                                                           <  8:266512/149‑1 (MQ=255)
                           gtgGCTGCTGGCGCGCAAACGCAAGGCCCAGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAAccc                                                                                                                         >  4:66919/1‑149 (MQ=255)
                            tgGCTGCTGGCGCGCAAACGCAAGGCCCAGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCt                                                                                                                        <  7:41443/149‑1 (MQ=255)
                            tgGCTGCTGGCGCGCAAACGCAAGGCCCAGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCt                                                                                                                        >  3:288662/1‑149 (MQ=255)
                               ctgctgGCGCGCAAACGCAAGGCCCAGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTc                                                                                                                     <  7:119080/149‑1 (MQ=255)
                                           aaaCGCAAGGCCCAGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGt                                                                                                         >  2:263558/1‑149 (MQ=255)
                                                cAAGGCCCAGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCg                                                                                                    <  1:162461/149‑1 (MQ=255)
                                                 aaGGCCCAGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCtgt                                                                                                            <  2:196242/140‑1 (MQ=255)
                                                 aaGGCCCAGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCtgt                                                                                                            >  1:196242/1‑140 (MQ=255)
                                                    gCCCAGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTgg                                                                                                          <  5:124169/139‑1 (MQ=255)
                                                    gCCCAGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTgg                                                                                                          >  6:124169/1‑139 (MQ=255)
                                                      ccAGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCgg                                                                                              >  5:72352/1‑149 (MQ=255)
                                                      ccAGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCgg                                                                                              >  6:312692/1‑149 (MQ=255)
                                                        aGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCt                                                                                            >  8:384666/1‑149 (MQ=255)
                                                        aGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCt                                                                                            <  4:268862/149‑1 (MQ=255)
                                                           aagaagCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCa                                                                                         <  8:138134/149‑1 (MQ=255)
                                                             gaagCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGc                                                                                       >  7:172333/1‑149 (MQ=255)
                                                              aagCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGACGCCGCCGAAGctct                                                                                                                                          <  3:8066/97‑4 (MQ=255)
                                                              aagCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGACGCCGCCGAAGctct                                                                                                                                          >  4:8066/1‑94 (MQ=255)
                                                               agCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTg                                                                                     >  3:56091/1‑149 (MQ=255)
                                                                gCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGc                                                                                    >  2:15943/1‑149 (MQ=255)
                                                                   gagaAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGt                                                                                 >  4:154993/1‑149 (MQ=255)
                                                                   gagaAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGt                                                                                 <  7:384666/149‑1 (MQ=255)
                                                                      aaGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGc                                                                              <  3:66919/149‑1 (MQ=255)
                                                                                gcATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGaaa                                                                      >  5:300596/1‑147 (MQ=255)
                                                                                 cATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAg                                                                   <  4:56091/149‑1 (MQ=255)
                                                                                  aTGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGt                                                                                                                                                   <  8:25708/68‑1 (MQ=255)
                                                                                  aTGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGt                                                                                                                                                   >  7:25708/1‑68 (MQ=255)
                                                                                  aTGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTc                                                                                <  2:19706/135‑1 (MQ=255)
                                                                                  aTGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTc                                                                                >  1:19706/1‑135 (MQ=255)
                                                                                  aTGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGt                                                                  >  8:320510/1‑149 (MQ=255)
                                                                                   tGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTg                                                                 <  7:172646/149‑1 (MQ=255)
                                                                                     gcgcGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTGGc                                                               <  4:288662/149‑1 (MQ=255)
                                                                                        cgGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTGGCAGa                                                            <  6:300596/149‑1 (MQ=255)
                                                                                         gggCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGt                                                                  <  2:29481/142‑1 (MQ=255)
                                                                                         gggCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGt                                                                  >  1:29481/1‑142 (MQ=255)
                                                                                              cTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTGGCAGAACCGGt                                                      <  8:172333/149‑1 (MQ=255)
                                                                                                 gaagaaGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCgg                                                                         <  8:34871/127‑1 (MQ=255)
                                                                                                 gaagaaGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCgg                                                                         >  7:34871/1‑127 (MQ=255)
                                                                                                 gaagaaGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTGGCAGAACCGGTCGc                                                   >  2:187069/1‑149 (MQ=255)
                                                                                                    gaagaGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTGGCAGAACCGGTCGcc                                                  >  1:66970/1‑147 (MQ=255)
                                                                                                     aagaGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTGGCAGAACCGGTCGCCGAg                                               <  7:320510/149‑1 (MQ=255)
                                                                                                        agCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTGACAGAACCGGTCGCCGAGgca                                            <  1:15943/149‑1 (MQ=255)
                                                                                                                gcAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGc                                                                                                      >  2:360384/1‑83 (MQ=255)
                                                                                                                gcAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGc                                                                                                      <  1:360384/83‑1 (MQ=255)
                                                                                                                  aGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGATGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTGGCAGAACCGGTCGCCGAGGCAGCACCCGAGc                                  >  5:208836/1‑149 (MQ=255)
                                                                                                                    gTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTGGCAGAACCGGTCGCCGAGGCAGCACCCGAGCCt                                >  5:189414/1‑149 (MQ=255)
                                                                                                                        cGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTGGCAGAACCGGTCGCCGAGGCAGCACCCGAGCCTGaa                             <  6:189414/148‑1 (MQ=255)
                                                                                                                         gACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTGGc                                                               <  7:92384/113‑1 (MQ=255)
                                                                                                                         gACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTGGc                                                               >  8:92384/1‑113 (MQ=255)
                                                                                                                           cAGCGATATCGAGAGCTTCGGAGTCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTGGCAGAACCGGTCGCCGAGGCAGCACCCGAGCCTGAACcac                         <  4:144056/149‑1 (MQ=255)
                                                                                                                               gATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTg                                                                 >  6:233537/1‑105 (MQ=255)
                                                                                                                               gATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTg                                                                 <  5:233537/105‑1 (MQ=255)
                                                                                                                                 tatCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTgg                                                                <  2:388830/104‑1 (MQ=255)
                                                                                                                                   tcgggggCTTCGACGCCGCCGAAGCTCTCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTGGCAGAACCGGTCGCCGAGGCAGCACCCGAGCCTGAACCACAAGCAGAc                 >  8:10519/7‑149 (MQ=255)
                                                                                                                                     gagaGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTGGCAGAACCGGTCGCCGAGGCAGCACCCGAGCCTGAACCACAAGCAGAccc               <  3:154993/149‑1 (MQ=255)
                                                                                                                                              ggcggcGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTGGCAGAACCGGTCGCCGAGGCAGCACCCGAGCCTGAACCACAAGCAGACCCGCAcgccgc      <  6:150554/149‑1 (MQ=255)
                                                                                                                                               gcggcgTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTGGCAGAACCGGTCGCCGAGGCAGCACCCGAGCCTGAACCACAAGCAGACCCGCACGCcgcg     >  4:30650/1‑149 (MQ=255)
                                                                                                                                                  gcgTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTGGCAGAACCGGTCGCCGAGGCAGCACCCGAGCCTGAACCACAAGCAGACCCGCACGCCGCgctg  >  6:342469/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CAGCATCCTGGTGCTGGCACTGCTGCTGTGGCTGCTGGCGCGCAAACGCAAGGCCCAGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTGGCAGAACCGGTCGCCGAGGCAGCACCCGAGCCTGAACCACAAGCAGACCCGCACGCCGCGCTG  >  NC_002947/2260600‑2260894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑