Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,372,083 G→A 9.4% intergenic (+35/+77) gdhB → / ← PP_2081 NAD‑specific glutamate dehydrogenase/phosphoenolpyruvate synthase regulatory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,372,0830GA 9.4% 141.0 / 4.8 53intergenic (+35/+77)gdhB/PP_2081NAD‑specific glutamate dehydrogenase/phosphoenolpyruvate synthase regulatory protein
Reads supporting (aligned to +/- strand):  ref base G (27/21);  new base A (2/3);  total (29/24)
Fisher's exact test for biased strand distribution p-value = 6.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.13e-01

GACGACCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCACCGA  >  NC_002947/2371941‑2372222
                                                                                                                                              |                                                                                                                                           
gacgacCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCg                                                                                                                                       >  8:34681/1‑149 (MQ=255)
gacgacCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCg                                                                                                                                       >  8:298612/1‑149 (MQ=255)
   gacCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCgggg                                                                                                                                    >  6:2809/1‑149 (MQ=255)
         cgcAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCTTTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCATCACTGCCGGGGCttt                                                                                                                                <  1:159242/147‑1 (MQ=255)
         cgcAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCATCACTGCCGGGGCttt                                                                                                                                <  7:326643/147‑1 (MQ=255)
            aaTGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCtttttttt                                                                                                                           <  6:301047/149‑1 (MQ=255)
                 cACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGATGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCACCGGCATCACTGCCGGGGCttg                                                                                                                                >  2:153467/1‑138 (MQ=255)
                 cACGGGCACTGACTATGCGATGTACGCGGGGGCCACCCTCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGGAAAACAAAGCCCCGGCATCACTGCCGGGGCttt                                                                                                                                <  1:153467/139‑1 (MQ=255)
                  aCGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGc                                                                                                                     >  6:222329/1‑149 (MQ=255)
                      gCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTgg                                                                                                                 >  4:5135/1‑149 (MQ=255)
                       cACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTggg                                                                                                                >  2:301276/1‑149 (MQ=255)
                         cTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTgggcg                                                                                                              <  4:359558/149‑1 (MQ=255)
                         cTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTgggcg                                                                                                              <  5:90364/149‑1 (MQ=255)
                            aCTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGCGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTgggcgg                                                                                                             <  3:5135/147‑1 (MQ=255)
                                tGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCAtt                                                                                                       >  6:108007/1‑149 (MQ=255)
                                   gATGTACGCGGTGGCCAACCGCGAGCTGGTGGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTcg                                                                                                    >  1:32303/1‑149 (MQ=255)
                                    cagctcgcgGTTGGCCACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTcgc                                                                                                   >  1:109191/6‑149 (MQ=255)
                                    aTGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTcgc                                                                                                   <  7:34681/149‑1 (MQ=255)
                                       tACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCggg                                                                                                >  4:79401/1‑149 (MQ=255)
                                       tACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCgg                                                                                                 <  5:2809/148‑1 (MQ=255)
                                                  cAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTTGGCGGGCCATTTCGCGGGTGAACCCGCTc                                                                                     <  1:239700/149‑1 (MQ=255)
                                                    aCCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCt                                                                                   <  5:222329/149‑1 (MQ=255)
                                                             tGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAgggggg                                                                          >  5:312914/1‑149 (MQ=255)
                                                                  gACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGggggcgg                                                                      <  3:79401/148‑1 (MQ=255)
                                                                   aCCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTAc                                                                                 <  4:406447/136‑1 (MQ=255)
                                                                   aCCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTAc                                                                                 >  3:406447/1‑136 (MQ=255)
                                                                          cATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGACCCCGCTCCTACAGGGGGGGCGGGGCTGTTAc                                                             <  2:109191/149‑1 (MQ=255)
                                                                          cATGAGCGGGCAGGCAGCGGGGGGGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTAc                                                             <  7:112551/149‑1 (MQ=255)
                                                                            tGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTACTACATGGGGGGCGGGGCTGTTACtt                                                           >  6:321604/1‑149 (MQ=255)
                                                                             gAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACtt                                                           >  6:379004/1‑148 (MQ=255)
                                                                               gCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGaa                                                        >  7:297444/1‑149 (MQ=255)
                                                                                 gggCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAAcc                                                      <  1:149570/149‑1 (MQ=255)
                                                                                    caggcagCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGCTGCCGGGGCTGTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGGTCGTACAGGGGGGGCGGGGCTGTTACTTGAACCGtg                                                   >  7:342328/1‑148 (MQ=255)
                                                                                             gtggtgCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGAGGGGCTGTTACTTGAACCTTCTCTCCCCGc                                          >  8:339273/1‑149 (MQ=255)
                                                                                                 tgCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCttt                                      <  2:32303/149‑1 (MQ=255)
                                                                                                  gCCGTCCTGCGCCTTGTAGTGCGGTCAAACCACCCCCCGGCCGGGCTGCCGGGGCTTTTTTTTGTAGGCCGGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACCGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCtttt                                     <  7:298612/149‑1 (MQ=255)
                                                                                                   ccGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCttttt                                    <  8:297444/149‑1 (MQ=255)
                                                                                                       ccTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGAc                                >  7:380637/1‑149 (MQ=255)
                                                                                                          gAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAg                             >  3:355391/1‑149 (MQ=255)
                                                                                                           aGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAg                             >  2:204897/1‑148 (MQ=255)
                                                                                                           aGCCTTGTAGGGAGGTAAAACAAAGCCCCGGCTGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACCGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAg                             <  5:108007/148‑1 (MQ=255)
                                                                                                               ttGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGGTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTGTGTCCCCGCCTTTTTCGACCAGGATCt                        >  7:186625/1‑149 (MQ=255)
                                                                                                                    gTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGAGGGGCTGTTACTTGAACCGTCTATCCACGCCTTTTTCGACCAGCATCTTggag                   >  4:253395/1‑147 (MQ=255)
                                                                                                                     tGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCAGGCCTTTTTGGACCAGGATCTTGGCg                   >  3:313892/1‑148 (MQ=255)
                                                                                                                     tGAGGTAAAACAAAGCCCCGGCAATGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTAATTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCg                   >  7:259589/1‑148 (MQ=255)
                                                                                                                               cAACGCCCCGGCAGGGATGCCGGGGCTTTTTTTTGTATGCGTGGGCGGGCCATTGCGCGGGTGACCCCGCTCCTACCGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTc        <  7:339273/149‑1 (MQ=255)
                                                                                                                                aaaGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGaa                                                        >  7:174309/1‑100 (MQ=255)
                                                                                                                                aaaGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGaa                                                        <  8:174309/100‑1 (MQ=255)
                                                                                                                                   gCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTCCAGGGGGGGCGGGGCTGTTACTTGAACCTTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCAcc    <  1:301276/149‑1 (MQ=255)
                                                                                                                                      ccGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCACCGa  >  5:368370/1‑148 (MQ=255)
                                                                                                                                      ccGGCAGCACTGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTAATTGAACCGTCTCTCCACGCCTTTTTCGCCCAGGATCTTGGAGGAAATCTCTTCCACCGa  >  7:252478/1‑148 (MQ=39)
                                                                                                                                             tGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCa                              <  6:237629/113‑1 (MQ=255)
                                                                                                                                             tGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCa                              >  5:237629/1‑113 (MQ=255)
                                                                                                                                              |                                                                                                                                           
GACGACCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCACCGA  >  NC_002947/2371941‑2372222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑