Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,396,427 C→G 8.3% R1077P (CGC→CCC) ‡ PP_2100 ← two‑component system sensor protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,396,4270CG 8.3% 183.1 / 4.0 59R1077P (CGC→CCC) ‡PP_2100two‑component system sensor protein
Reads supporting (aligned to +/- strand):  ref base C (23/31);  new base G (3/2);  total (26/33)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTGCGGCGTACTTCCGGCTGTGCGTTGGCGGTATAGCCGAGGATGGTGCAGGGTGGTCGCTTGCCATGGCGTTCTTCGGCGC  >  NC_002947/2396282‑2396574
                                                                                                                                                 |                                                                                                                                                   
cAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCgct                                                                                                                                                  >  2:95125/1‑149 (MQ=255)
   gTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCgctggc                                                                                                                                               <  4:68616/149‑1 (MQ=255)
     cGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCgctggct                                                                                                                                              >  5:319682/1‑148 (MQ=255)
      ggCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGa                                                                                                                                            <  6:111007/149‑1 (MQ=255)
        ccTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAgg                                                                                                                                          <  4:169567/149‑1 (MQ=255)
         cTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAggg                                                                                                                                         >  1:231426/1‑149 (MQ=255)
          tGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGt                                                                                                                                        >  2:180192/1‑149 (MQ=255)
              ggCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCt                                                                                                                                     >  6:173846/1‑148 (MQ=255)
                     gTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGc                                                                                                                                <  5:265220/146‑1 (MQ=255)
                      tGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGAt                                                                                                                            <  6:319682/149‑1 (MQ=255)
                      tGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGAt                                                                                                                            <  2:337284/149‑1 (MQ=255)
                      tGCAAGGTATCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGAt                                                                                                                            <  1:277680/149‑1 (MQ=255)
                       gCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATg                                                                                                                           >  6:136173/1‑149 (MQ=255)
                       gCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATg                                                                                                                           >  4:243127/1‑149 (MQ=255)
                        cAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATgg                                                                                                                          >  2:129959/1‑149 (MQ=255)
                        cAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATg                                                                                                                           <  1:180192/148‑1 (MQ=255)
                          aGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGc                                                                                                                        <  5:173846/149‑1 (MQ=255)
                            gTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCtt                                                                                                                      <  2:231426/149‑1 (MQ=255)
                                 cAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGGGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGc                                                                                                                  <  1:129959/148‑1 (MQ=255)
                                   ggAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAAGCCCATCAGCCTGAGCacc                                                                                                               <  2:1187/149‑3 (MQ=255)
                                     aaGCGCTGACGGTGATCCGTGTCAGGCCCGACCACGGCGGCCAGGCCGTCAAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAAGCCCATCAGCCTGAGCaccct                                                                                                             <  3:303078/149‑5 (MQ=255)
                                      aGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAg                                                                                                            <  8:274452/149‑1 (MQ=255)
                                               ggTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAgg                                                                                                                                          <  8:97478/110‑1 (MQ=255)
                                               ggTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAgg                                                                                                                                          >  7:97478/1‑110 (MQ=255)
                                                     ccGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCtt                                                                                                                      <  6:218380/124‑1 (MQ=255)
                                                     ccGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCtt                                                                                                                      >  5:218380/1‑124 (MQ=255)
                                                           cAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAgg                                                                                       >  8:370613/1‑149 (MQ=255)
                                                           cAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAgg                                                                                       >  3:112220/1‑149 (MQ=255)
                                                                 cGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGATTGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTg                                                                                 <  2:339566/149‑1 (MQ=255)
                                                                        ggcggcCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGGGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTGCGGCGTa                                                                          <  5:418051/149‑1 (MQ=255)
                                                                        ggcggcCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCcgg                                                                                               >  2:315471/1‑128 (MQ=255)
                                                                        ggcggcCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCcgg                                                                                               <  1:315471/128‑1 (MQ=255)
                                                                        ggcggcCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTGCGGCGt                                                                           >  8:36391/1‑148 (MQ=255)
                                                                          cggcCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTGCGGCGTACt                                                                        >  7:168863/1‑149 (MQ=255)
                                                                               aGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACa                                                                                                             >  1:15080/1‑107 (MQ=255)
                                                                               aGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACa                                                                                                             <  2:15080/107‑1 (MQ=255)
                                                                                  ccGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTgcggc                                                                             <  2:383682/136‑1 (MQ=255)
                                                                                           tGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTGCGGCGTACTTCCGGCTGTGCGTTGGc                                                       <  7:370613/149‑1 (MQ=255)
                                                                                             gTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTGCGGCGTACTTCCGGCTGTGCGTTg                                                         <  8:168863/145‑1 (MQ=255)
                                                                                             gTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTGCGGCGTACTTCCGGCTGTGCGTTGGc                                                       <  4:112220/147‑1 (MQ=255)
                                                                                                         tCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGTGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTGCGGCGTACTTCCGGCTGTGCGTTGGCGGTATAGCCGAGGa                                         <  5:136173/149‑1 (MQ=255)
                                                                                                           gggggCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTGCGGCGTACTTCCGGCTGTGCGTTGGCGGTATAGCCGAGGATg                                       >  3:284024/1‑149 (MQ=255)
                                                                                                            ggggCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCGGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTGCGGCGTACTTCCGGCTGTGCGTTGGCGGTATAGCCGAGGATgg                                      >  6:384821/1‑149 (MQ=255)
                                                                                                            ggggCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTGCGGCGTACTTCCgg                                                                   >  6:409943/1‑120 (MQ=255)
                                                                                                            ggggCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTGCGGCGTACTTCCgg                                                                   <  5:409943/120‑1 (MQ=255)
                                                                                                                cGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCGGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTGCGGCGTACTTCCGGCTGTGCGTTGGCGGTATAGCCGAGGATGGTGCa                                  <  6:91098/149‑1 (MQ=255)
                                                                                                                    gCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTGCGGCGTACTTCCGGCTGTGCGTTGGCGGTATAGCCGAGGATGGTGCAGggt                              >  2:235396/1‑149 (MQ=255)
                                                                                                                      tGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCAATGCCGGCGGTCAGGCAATTGCGGCGTAATTCCGGCTGTGCGTTGGCGGTATAGCCGAGGATGGTGCAGggtgt                            >  8:259056/1‑148 (MQ=255)
                                                                                                                        gcggcgCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCGGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTGCGGCGTACTTCCGGCTGTGCGTTGGCGGTATAGCCGAGGATGGTGCAGGGTGGTc                          <  5:384821/149‑1 (MQ=255)
                                                                                                                             gcgGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTGCGGCGTACTTCCGGCTGTGCGTTGGCGGTATAGCCGAGGATGGTGCAGGGTGGTCGCTTg                     <  7:36391/149‑1 (MQ=255)
                                                                                                                                  cGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTGCGGCGTACTTCCGGCTGTGCGTTGGCGGTATAGCCGAGGATGGTGCAGGGTGGTCGCTTGCCATg                <  4:284024/149‑1 (MQ=255)
                                                                                                                                       ctcagccagcGCCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTGCGGCGTACTTCCGGCTGTGCGTTGGCGGTATAGCCGAGGATGGt                                     <  2:244385/121‑1 (MQ=255)
                                                                                                                                       ctcagccagcGCCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTGCGGCGTACTTCCGGCTGTGCGTTGGCGGTATAGCCGAGGATGGt                                     >  1:244385/3‑123 (MQ=255)
                                                                                                                                        tcagccagcGCCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTGCGGCGTACTTCCGGCTGTGCGTTGGCGGTATAGCCGAGGATGGTGCAGggtggt                           >  7:183712/2‑132 (MQ=255)
                                                                                                                                        tcagccagcGCCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTGCGGCGTACTTCCGGCTGTGCGTTGGCGGTATAGCCGAGGATGGTGCAGggtggt                           <  8:183712/131‑1 (MQ=255)
                                                                                                                                        tcagccagcGCCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTGCGGCGTACTTCCGGCTGTGCGTTGGCGGTATAGCCGAGGATGGTGCAGGGTGGTCGCTTGCCATGGCGttc          >  7:154039/2‑149 (MQ=255)
                                                                                                                                        ccagccagGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGCCAGTCGTCCATGCCGGCGGTCAGGCACTTGCGGCGTACTTCCGGCTGTGCGTTGGCGGTATAGCCGAGGATGGTGCAGGGTGGTCGCTTGCCATGGCGttc          >  3:32010/1‑149 (MQ=255)
                                                                                                                                         cagccagGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTGCGGCGTACTTCCGGCTGTGCGTTGGCGGTATAGCCGAGGATGGTGCAGGGTGGTCGCTTGCCATgg               >  7:316973/1‑143 (MQ=255)
                                                                                                                                         cagccagGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTGCGGCGTACTTCCGGCTGTGCGTTGGCGGTATAGCCGAGGATGGTGCAGGGTGGTCGCTTGCCATgg               <  8:316973/143‑1 (MQ=255)
                                                                                                                                                gcgcGGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGGCAGGCACTTGCGGCGTACTTCCGGCTGTGCGTTGGCGGTATAGCCGAGGATGGTGCAGGGTGGTCGCTTGCCATGGCGTTCTTCGgcgc  <  3:243127/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTGCGGCGTACTTCCGGCTGTGCGTTGGCGGTATAGCCGAGGATGGTGCAGGGTGGTCGCTTGCCATGGCGTTCTTCGGCGC  >  NC_002947/2396282‑2396574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑