Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,445,359 G→C 10.1% A51A (GCC→GCG)  lexA‑I ← transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,445,3590GC10.1% 158.5 / 7.3 52A51A (GCC→GCG) lexA‑Itranscriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (24/22);  new base C (3/2);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01

TTGCTCGATGTGCTGTTCGGCAAGAATCGGCGCACCGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGTTGTCTTCCAGGCAGCGCTTGATGAACGCGAGAATTTCGGCTTGGCGTG  >  NC_002947/2445212‑2445495
                                                                                                                                                   |                                                                                                                                        
ttggtggATGTGGTGTTCTGCAATAATGGGCGCTCCGTCATCGACCCGGCCTATGATGGGCAGGCCGGCTTCTTCTGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCgg                                                                                                                                          <  8:314890/142‑1 (MQ=255)
   cTCGATGTGCTGTTCGGCAAGAATCGGCGCACCGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCaa                                                                                                                                      <  8:164820/149‑1 (MQ=255)
      gATGTGCTGTTCGGCAAGAATCGGCGCACCGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAggg                                                                                                                                   >  8:390951/1‑149 (MQ=255)
       aTGTGCTGTTCGGCAAGAATCGGCGCACCGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGc                                                                                                                                  <  6:32894/149‑1 (MQ=255)
        tgtgCTGTTCGGCAAGAATCGGCGCACCGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGcc                                                                                                                                 >  4:76150/1‑149 (MQ=255)
          tgCTGTTCGGCAAGAATCGGCGCACCGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTCCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCtt                                                                                                                               <  2:198969/149‑1 (MQ=255)
           gCTGTTCGGCAAGAATCGGCGCACCGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCtt                                                                                                                               >  1:68788/1‑148 (MQ=255)
            cTGTTCGGCAAGAATCGGCGCACCGGCAGCGACCCGTCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCCCGCAAGGGcgcga                                                                                                                              <  3:54535/148‑5 (MQ=255)
               ttCGGCAAGAATCGGCGCCCCGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAg                                                                                                                            <  2:170021/147‑1 (MQ=255)
                  ggCAAGAATCGGCGCACCGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCCCGCAAGGGcg                                                                                                                                 >  8:79248/1‑138 (MQ=255)
                  ggCAAGAATCGGCGCACCGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCCCGCAAGGGcg                                                                                                                                 <  7:79248/139‑2 (MQ=255)
                  ggCAAGAATCGGCGCACCGGCAGCGACCCGGCCGATGATGGGCAGACCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGct                                                                                                                       >  3:40512/1‑149 (MQ=255)
                    cAAGAATCGGCGCACCGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCtt                                                                                                                               >  3:299735/1‑139 (MQ=255)
                    cAAGAATCGGCGCACCGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCtt                                                                                                                               <  4:299735/139‑1 (MQ=255)
                     aaGAATCGGCGCACCGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGcc                                                                                                                                 >  5:370844/1‑136 (MQ=255)
                     aaGAATCGGCGCACCGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGcc                                                                                                                                 <  6:370844/136‑1 (MQ=255)
                        aaTCGGCGCACCGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTcgg                                                                                                                 >  6:239509/1‑149 (MQ=255)
                             gcgcACCGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTcggcg                                                                                                               <  4:184543/146‑1 (MQ=255)
                             gcgcACCGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTcggcg                                                                                                               >  3:184543/1‑146 (MQ=255)
                                 aCCGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGGGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTgg                                                                                                        <  8:386886/149‑1 (MQ=255)
                                 aCCGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTgg                                                                                                        <  2:68788/149‑1 (MQ=255)
                                  ccGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTggg                                                                                                       <  7:390951/149‑1 (MQ=255)
                                              cGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCTTCGGCATTGGGCGACTTGAAGcc                                                                                           <  7:60960/149‑1 (MQ=255)
                                              cGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGcc                                                                                           <  5:224410/149‑1 (MQ=255)
                                              cGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGcc                                                                                           >  6:224410/1‑149 (MQ=255)
                                                gCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCa                                                                                         <  1:381510/149‑1 (MQ=255)
                                                       atgGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCt                                                                                   <  5:239509/148‑1 (MQ=255)
                                                        tgGGCAGGCCGGATTCTTCTGCCTTGGCTTCCAGGCCAGGTATGCGGATGCCGGGGGAAGCGCCCGTCGTCATTTCGATCGCGCCCTTGCGTGCAAGGTCCTTGATGTGCTCCTCGGCTGCATTGGGCGACTTGAAGCCCAGCTCCTg                                                                                  <  1:31430/148‑1 (MQ=255)
                                                                  ggCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCgg                                                                       <  3:76150/149‑1 (MQ=255)
                                                                          cAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCgg                                                                  <  5:380207/146‑1 (MQ=255)
                                                                            gCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAgg                                                             >  8:36057/1‑149 (MQ=255)
                                                                             ccTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGc                                                            >  5:62981/1‑149 (MQ=255)
                                                                              cTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCg                                                           >  6:182591/1‑149 (MQ=255)
                                                                                  gCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGaa                                                       <  6:62981/149‑1 (MQ=255)
                                                                                     tCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGcc                                                    >  4:236872/1‑149 (MQ=255)
                                                                                         ggccaggGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGTTg                                                >  7:4750/1‑149 (MQ=255)
                                                                                                     ggatgcCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGTTGTCTTCCAGGCAg                                    >  6:22446/1‑149 (MQ=255)
                                                                                                      gatgcCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGTTGTCTTCCAGGCAgc                                   >  5:157502/1‑149 (MQ=255)
                                                                                                           cgcgGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGTTGTCTTCCAGGCAGCGCtt                               >  3:29499/1‑148 (MQ=255)
                                                                                                            gcgGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGTTGTCTTCCAGGCAGCGCTtga                             <  4:40512/149‑1 (MQ=255)
                                                                                                                gAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTggg                                                                                                       >  4:21995/1‑71 (MQ=255)
                                                                                                                    cgcCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGTTGTCTTCCAGGCAGCGCTtg                              <  7:38237/140‑1 (MQ=255)
                                                                                                                    cgcCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGTTGTCTTCCAGGCAGCGCTtg                              >  8:38237/1‑140 (MQ=255)
                                                                                                                               aTTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGTTGTCTTCCAGGCAGCGCTTGATGAACGCGAGAATTTCGGc          >  6:145958/1‑149 (MQ=255)
                                                                                                                               aTTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGTTGTCTTCCAGGCAGCGCTTGATGAACGCGAGAATTTCGGc          >  3:79151/1‑149 (MQ=255)
                                                                                                                                 ttCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCg                                                   <  6:35720/106‑1 (MQ=255)
                                                                                                                                 ttCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCg                                                   >  5:35720/1‑106 (MQ=255)
                                                                                                                                  tCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGTTGTCTTCCAGGCAGCGCTTGATGAACGCGAGAATTTCGGCTtg       >  5:115046/1‑149 (MQ=255)
                                                                                                                                   cGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGTTGTCTTCCAGGCAGCGCTTGATGAACGCGAGAATTTCGGCTtgg      >  6:131038/1‑149 (MQ=255)
                                                                                                                                   cGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGTTGTCTTCCAGGCAGCGCTTGATGAACGCGAGAATTTCGGCTtg       <  7:36057/148‑1 (MQ=255)
                                                                                                                                    ctcaaggcCCTTGCCCGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGTTGTCTTCCAGGCAGCGCTTGATGAACGCGAGAATTTCGGCTtggc     >  1:151503/7‑149 (MQ=255)
                                                                                                                                       aaggcCCTTGCCCGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGTTGTCTTCCAGGCAGCGCTTGATGAACGCGAGAATTTCGGCTtggcgtg  >  4:393118/4‑149 (MQ=255)
                                                                                                                                        gcgcCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGTTGTCTTCCAGGCAGCGCTTGATGAACGCGAGAATTTCGGCTtggcgtg  <  5:145958/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                        
TTGCTCGATGTGCTGTTCGGCAAGAATCGGCGCACCGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGTTGTCTTCCAGGCAGCGCTTGATGAACGCGAGAATTTCGGCTTGGCGTG  >  NC_002947/2445212‑2445495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑