Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,460,377 G→A 6.0% R143C (CGC→TGC)  PP_2153 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,460,3770GA 6.0% 197.5 / 2.2 66R143C (CGC→TGC) PP_2153hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (35/27);  new base A (2/2);  total (37/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.00e-01

TTCAGGGTGTTCTCGGGTGCTTCGCCCTTGGCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAA‑CTCGGTACCGACCTCGAAGCCGCCCTCCGGGCGAGGGTCGCAATGGGTGACCTTGCCGCGCAGCAGCAGGCCGTGGGCGCGCGGCATCAGCAC  >  NC_002947/2460234‑2460524
                                                                                                                                               |                                                                                                                                                    
ttCAGGGTGTTCTCGGGTGCTTCGCCCTTGGCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTg                                                                                                                                                   >  4:373299/1‑147 (MQ=255)
  cAGGGTGTTCTCGGGTGCTTCGCCCTTGGCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAg                                                                                                                                                >  1:275118/1‑148 (MQ=255)
     ggTGTTCTCGGGTGCTTCGCCCTTGGCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATg                                                                                                                                            >  2:133211/1‑149 (MQ=255)
         ttCTCGGGTGCTTCGCCCTTGGCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATc                                                                                                                                        <  2:275118/149‑1 (MQ=255)
            tcGGGTGCTTCGCCCTTGGCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCggg                                                                                                                                     >  4:360138/1‑149 (MQ=255)
            tcGGGTGCTTCGCCCTTGGCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCggg                                                                                                                                     >  8:224189/1‑149 (MQ=255)
            tcGGGTGCTTCGCCCTTGGCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCggg                                                                                                                                     >  7:84020/1‑149 (MQ=255)
            tcGGGTGCTTCGCCCTTGGCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCggg                                                                                                                                     >  6:71064/1‑149 (MQ=255)
              gggTGCTTCGCCCTTGGCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTAGTTGTTGCTGGCGCTGCAGGATGTATCGGGc                                                                                                                                    >  2:303992/1‑148 (MQ=255)
                gTGCTTCGCCCTTGGCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCgg                                                                                                                                      >  5:330439/1‑144 (MQ=255)
                gTGCTTCGCCCTTGGCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCgg                                                                                                                                      <  6:330439/144‑1 (MQ=255)
                   cTTCGCCCTTGGCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCcagcag                                                                                                                              <  7:224189/149‑1 (MQ=255)
                      cGCCCTTGGCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGtt                                                                                                                            <  1:133211/148‑1 (MQ=255)
                        cccTTGGCGGCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCATGAGGCGGGGTCGTTCAGTTCGAGCTGCTGGCGCCGTTGTTGTTGGTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTc                                                                                                                         <  5:147985/149‑1 (MQ=255)
                             ggCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGGTCAGGAGGCGGGGGCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGtt                                                                                                                      <  4:8997/147‑1 (MQ=255)
                             ggCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGGCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTCTCGGGCCAGCAGTTGTCGTTg                                                                                                                     <  3:271489/148‑1 (MQ=255)
                              gcgcCGCGATGGCCGTAGATCAGGTTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTgg                                                                                                                    <  8:132383/148‑1 (MQ=255)
                              gcgcCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTgg                                                                                                                    <  3:360138/148‑1 (MQ=255)
                              gcgcCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCACGATGTATCGGGCCAGCAGTTGTCGTTgg                                                                                                                    <  1:230408/148‑1 (MQ=255)
                                     gATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTTCTGGCGCTGCAGGATGTATCTGGAcagca                                                                                                                               >  8:288119/1‑130 (MQ=255)
                                     gATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCcagca                                                                                                                               <  7:288119/130‑1 (MQ=255)
                                           cGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTc                                                                                                      <  8:84020/149‑1 (MQ=255)
                                            gTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGAt                                                                                                                                             >  5:92372/1‑109 (MQ=255)
                                            gTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGAt                                                                                                                                             <  6:92372/109‑1 (MQ=255)
                                               gATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGAt                                                                                                   >  4:202458/1‑148 (MQ=255)
                                                 tCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGa                                                                                                 >  8:63539/1‑148 (MQ=255)
                                                          cgttcgttCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAA‑CTCGGTAc                                                                                       <  3:327543/148‑1 (MQ=255)
                                                          cgttcgttCCTTCAAATCAGGGAAAAATTGGATCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAA‑CTCGGTAc                                                                                       >  4:327543/1‑148 (MQ=255)
                                                           gttcgttcCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAA‑CTCGGTAcc                                                                                      >  1:162678/1‑148 (MQ=255)
                                                                      cAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGAGTCGGTCATGTCGATGAA‑CTCGGTACCGACCTCGAAgcc                                                                          >  5:238192/1‑149 (MQ=255)
                                                                          tCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAA‑CTCGGTACCGACCTCGAAGCCGccc                                                                      >  1:52341/1‑149 (MQ=255)
                                                                              ggAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGTGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAA‑CTCGGTACCGACCTCGAAGCCGCCCTCCg                                                                  >  4:196893/1‑149 (MQ=255)
                                                                              ggAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAA‑CTCGGTACCGAc                                                                                   <  3:248441/132‑1 (MQ=255)
                                                                              ggAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAA‑CTCGGTACCGAc                                                                                   >  4:248441/1‑132 (MQ=255)
                                                                              ggAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAA‑CTCGGTACCGACCTCGAAGCCGCCCTCCg                                                                  >  5:102285/1‑149 (MQ=255)
                                                                              ggAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAA‑CTCGGTACCGACCTCGAAGCCGCCCTCCg                                                                  >  3:140149/1‑149 (MQ=255)
                                                                              ggAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGGTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGCTCATGTCGATGAA‑CTCGGTACCGACCTCGAAGCCGCCCTCCg                                                                  >  2:360586/1‑149 (MQ=255)
                                                                                aaaaaTTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAACCTCGGTACCGACCTCGAAGCCGCCCTCCgg                                                                 <  1:30215/149‑1 (MQ=255)
                                                                                aaaaaTTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAA‑CTCGGTACCGACCTCGAAGCCGCCCTCCggg                                                                <  7:63539/149‑1 (MQ=255)
                                                                                 aaaaTTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAA‑CTCGGTACCGACCTCGAAGCCGCCCTCCGGGc                                                               <  2:409292/149‑1 (MQ=255)
                                                                                 aaaaTTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAA‑CTCGGTACCGACCTCGAAGCCGCCCTCCGGGc                                                               >  5:306040/1‑149 (MQ=255)
                                                                                  aaaTTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAA‑CTCGGTACCGACCTCGAAGCCGCCCTc                                                                    <  2:123990/143‑1 (MQ=255)
                                                                                  aaaTTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAA‑CTCGGTACCGACCTCGAAGCCGCCCTc                                                                    >  1:123990/1‑143 (MQ=255)
                                                                                    aTTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGAt                                                                                                   <  5:69302/111‑1 (MQ=255)
                                                                                    aTTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGAt                                                                                                   >  6:69302/1‑111 (MQ=255)
                                                                                         cTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCAt                                                                                                         >  2:241633/1‑100 (MQ=255)
                                                                                         cTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCAt                                                                                                         <  1:241633/100‑1 (MQ=255)
                                                                                          tCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAA‑CTCGGTACCGACCTCGAAGCCGCCCTCCGGGCGAGGGTCg                                                       >  4:298041/1‑148 (MQ=255)
                                                                                            aggaggCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAA‑CTCGGTACCGACCTCGAAGCCGCCCTCCGGGCGAGGGTCGCaa                                                    <  3:373299/149‑1 (MQ=255)
                                                                                              gaggCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAA‑CTCGGTACCGACCTCGAAGCCGCCCTCCGGGCGAGGGTCGCAATg                                                  >  8:352244/1‑149 (MQ=255)
                                                                                              gaggCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAA‑CTCGGTACCGACCTCGAAGCCGCCCTCCGGGCGAGGGTCGCAATg                                                  <  4:140149/149‑1 (MQ=255)
                                                                                               aggCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAA‑CTCGGTACCGACCTCGAAGCCGCCCTCCGGGCGAGGGTCGCAATgg                                                 <  2:164693/149‑1 (MQ=255)
                                                                                                           tCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAA‑CTCGGTACCGACCTCGAAGCCGCCCTCCGGGCGAGGGTCGCAATGGGTGACCtt                                         <  8:54056/145‑1 (MQ=255)
                                                                                                           tCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAA‑CTCGGTACCGACCTCGAAGCCGCCCTCCGGGCGAGGGTCGCAATGGGTGACCtt                                         >  7:54056/1‑145 (MQ=255)
                                                                                                             aGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAA‑CTCGGTACCGACCTCGAAGCCGCCCTCCGGGCGAGGGTCGCAATGGGTGACCTTGCcgcg                                   >  5:386983/1‑149 (MQ=255)
                                                                                                               ttCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCTGTCATGTCGATGAA‑CTCGGTACCGACCTCGAAGCCGCCCTCCGGGCGAGGGTCGCAATGGGTGACCTTGCCGCgca                                 <  6:238192/149‑1 (MQ=255)
                                                                                                                     ctgctgGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAA‑CTCGGTACCGACCTCGAAGCCGCCCTCCGGGCGAGGGTCGCAATGGGTGACCTTGCCGCgcagcagca                           <  3:202458/149‑1 (MQ=255)
                                                                                                                                gttgttgttGCTGGCACTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGa                                                                                                    <  1:330831/66‑1 (MQ=255)
                                                                                                                                gttgttgttGCTGGCACTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGa                                                                                                    >  4:349698/1‑66 (MQ=255)
                                                                                                                                gttgttgttGCTGGCACTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGa                                                                                                    >  2:330831/1‑66 (MQ=255)
                                                                                                                                gttgttgttGCTGGCACTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGa                                                                                                    <  3:349698/66‑1 (MQ=255)
                                                                                                                                   gttgttgCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTGCTTGGGCGTCGGTCATGTCGATGAA‑CTCGGTACCGACCTCGAAGCCGCCCGCCGGGCGAGGGTCGCAATGGGTGACCTTGCCGCGCAGCACCAGGCCGTGGgcgcgc             >  5:96877/1‑149 (MQ=255)
                                                                                                                                       ttgCTTGAGCTGCAGGAACTATCGGGCCAGCACTTGACTTAGGGCGTAAGTCATGTAGATGAA‑CTCCGTACAGACCACCAAGCCGCCCTCCGGGCGAGGGTCGCAATGCGTCACATAGCCGCCAACCAACAAGCCTTGGGCCCGCGGc          >  2:291483/1‑148 (MQ=255)
                                                                                                                                          cTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAA‑CTCGGTACCGACCTCGAAGCCGCCCTCCGGGCGAGGGTCGCAATGGGTGACCTTGCCGCGCAGCAGCAGGCCGTGGGCGCGCGGCATCa      >  7:278882/1‑149 (MQ=255)
                                                                                                                                           tGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAA‑CTCGGTACCGACCTCGAAGCCGCCCTCCGGGCGAGGGTCGCAATGGGTGACCTTGCCGCGCAGCAGCAGGCCGTGGGCGCGCGGCATCAg     >  1:165688/1‑149 (MQ=255)
                                                                                                                                            ggCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAA‑CTCGGTACCGACCTCGAAGCCGCCCTCCGGGCGAGGGTCGCAATGGGTGACCTTGCCGCGCAGCAGCAGGCCGTGGGCGCGCGGCATCAg     >  7:76799/1‑148 (MQ=255)
                                                                                                                                              cgcTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAA‑CTCGGTACCGACCTCGAAGCCGCCCTCCGGGCGAGGGTCGCAATGGGTGACCTTGCCGCGCAGCAGCAGGCCGTGGGCGCGCGGCATCAGCAc  <  6:386983/149‑1 (MQ=255)
                                                                                                                                               gcTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAA‑CTCGGTACCGACCTCGAAGCCGCCCTCCGGGCGAGGGTCGCAATGGGTGACCTTGCCGCGCAGCAGCAGGCCGTGGGCGCGCGGCATCAGCAc  >  6:39128/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
TTCAGGGTGTTCTCGGGTGCTTCGCCCTTGGCGCCGCGATGGCCGTAGATCAGGGTCACGTTCGTTCCTTCAAATCAGGGAAAAATTGGCTCAGGAGGCGGGGTCGTTCAGTTCCAGCTGCTGGCGCCGTTGTTGTTGCTGGCGCTGCAGGATGTATCGGGCCAGCAGTTGTCGTTGGGCGTCGGTCATGTCGATGAA‑CTCGGTACCGACCTCGAAGCCGCCCTCCGGGCGAGGGTCGCAATGGGTGACCTTGCCGCGCAGCAGCAGGCCGTGGGCGCGCGGCATCAGCAC  >  NC_002947/2460234‑2460524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑