| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 351,749 | C→T | 7.4% | Q79* (CAG→TAG) ‡ | hisA → | phosphoribosylformimino‑5‑aminoimidazole carboxamide ribonucleotide isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 351,749 | 0 | C | T | 7.4% | 240.1 / 6.4 | 81 | Q79* (CAG→TAG) ‡ | hisA | phosphoribosylformimino‑5‑aminoimidazole carboxamide ribonucleotide isomerase |
| Reads supporting (aligned to +/- strand): ref base C (43/32); new base T (3/3); total (46/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01 | |||||||||||
ACCCGGTGAGCATGGCCGCCAAGTGGGTCGAGGGTGGCTGCCGCCGCCTGCACCTGGTCGACCTCAACGGCGCCTTCGAAGGCCAGCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTTCGTCGCTGAAGCGTGCAAAGCCTTCCCGGGCAAGGTCATCGTCG > NC_002947/351606‑351896 | acCCGGTGAGCATGGCCGCCAAGTGGGTCGAGGGTGGCTGCCGCCGCCTGCACCTGGTCGACCTCAACGGCGCCTTCGAAGGCCAGCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATc < 2:392101/149‑1 (MQ=255) ccGGTGAGCATGGCCGCCAAGTGGGTCGAGGGTGGCTGCCGCCGCCTGCACCTGGTCGACCTCAACGGCGCCTTCGAAGGCCAGCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGACCCTGCCGATCCAGATcgg > 5:71803/1‑149 (MQ=255) cGGTGAGCATGGCCGCCAAGTGGGTCGAGGGTGGCTGCCGCCGCCTGCACCTGGTCGACCTCAACGGCGCCTTCGAAGGCCAGCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATcggc < 6:84628/149‑1 (MQ=255) aGCATGGCCGCCAAGTGGGTCGAGGGTGGCTGCCGCCGCCTGCACCTGGTCGACCTCAACGGCGCCTTCGAAGGCCAGCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATcggcggcg < 1:266848/148‑1 (MQ=255) cATGGCCGCCAAGTGGGTCGAGGGTGGCTGCCGCCGCCTGCACCTGGTCGACCTCAACGGCGCCTTCGAAGGCCAGCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTa > 7:72902/1‑149 (MQ=255) ggCCGCCAAGTGGGTCGAGGGTGGCTGCCGCCGCCTGCACCTGGTCGACCTCAACGGCGCCTTCGAAGGCCAGCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCTGGATcggcag < 8:344540/141‑3 (MQ=255) ggCCGCCAAGTGGGTCGAGGGTGGCTGCCGCCGCCTGCACCTGGTCGACCTCAACGGCGCCTTCGAAGGCCAGCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCTGGATcggcag > 7:344540/1‑139 (MQ=255) cgccAAGTGGGTCGAGGGTGGCTGCCGCCGCCTGCACCTGGTCGACCTCAACGGCGCCTTCGAAGGCCAGCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGtt > 6:158273/1‑149 (MQ=255) gccAAGTGGGTCGAGGGTGGCTGCCGCCGCCTGCACCTGGTCGACCTCAACGGCGCCTTCGAAGGCCAGCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTc < 8:50788/149‑1 (MQ=255) ggTCGAGGGTGGCTGCCGCCGCCTGCACCTGGTCGACCTCAACGGCGCCTTCGAAGGCCAGCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGaaa > 3:381334/1‑149 (MQ=255) tCGAGGGTGGCTGCCGCCGCCTGCACCTGGTCGACCTCAACGGCGCCTTCGAAGGCCAGCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAAc > 4:390262/1‑148 (MQ=255) ggTGGCTGCCGCCGCCTGCACCTGGTCGACCTCAACGGCGCCTTCGAAGGCCAGCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCg > 8:166217/1‑148 (MQ=255) gCTGCCGCCGCCTGCACCTGGTCGACCTCAACGGCGCCTTCGAAGGCCAGCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAaca < 3:316364/148‑1 (MQ=255) cgccgccTGCACCTGGTCGACCTCAACGGCGCCTTCGAAGGCCAGCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTAGCTGGAAACCATCGAACACTACGt < 7:257104/149‑1 (MQ=255) cgccTGCACCTGGTCGACCTCAACGGCGCCTTCGAAGGCCAGCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCaa < 1:365681/149‑1 (MQ=255) gccTGCACCTGGTCGACCTCAACGGCGCCTTCGAAGGCCAGCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAg > 1:10737/1‑149 (MQ=255) ccTGCACCTGGTCGACCTCAACGGCGCCTTCGAAGGCCAGCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGAGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAgg > 7:96078/1‑149 (MQ=255) tGCACCTGGTCGACCTCAACGGCGCCTTCGAAGGCCAGCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCt > 8:42162/1‑149 (MQ=255) gCACCTGGTCGACCTCAACGGCGCCTTCGAAGGCCAGCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTg < 4:381334/149‑1 (MQ=255) aCCTGGTCGACCTCAACGGCGCCTTCGAAGGCCAGCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGc < 7:166217/149‑1 (MQ=255) cTGGTCGACCTCAACGGCGCCTTCGAAGGCCAGCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGt > 4:120209/1‑149 (MQ=255) tGGTCGACCTCAACGGCGCCTTCGAAGGCCAGCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATc < 5:251412/125‑1 (MQ=255) tGGTCGACCTCAACGGCGCCTTCGAAGGCCAGCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATc > 6:251412/1‑125 (MQ=255) gACCTCAACGGCGCCTTCGAAGGCCAGCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCt < 7:190479/148‑1 (MQ=255) cTCAACGGCGCCTTCGAAGGCCAGCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGt < 7:42162/149‑1 (MQ=255) cTCAACGGCGCCTTCGAAGGCCAGCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCCATCCAGATCGGCGGCGGTAGGTCGCGTTAGCATGAAACCATACAACACAACGTAAAAGCTGGCCTCatcaa > 1:131696/1‑145 (MQ=255) gcgcCTTCGAAGGCCAGCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGatcatc < 8:72902/149‑1 (MQ=255) ccTTCGAAGGCCAGCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGc < 2:10737/149‑1 (MQ=255) cTTCGAAGGCCAGCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGtt < 3:396860/92‑1 (MQ=255) cTTCGAAGGCCAGCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGtt > 4:396860/1‑92 (MQ=255) cAGCCGGTCAATGGTGAAGTTGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGatcatc < 6:403496/135‑1 (MQ=255) cAGCCGGTCAATGGTGAAGTTGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGatcatc > 5:403496/1‑135 (MQ=255) cAGCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCg > 8:194259/1‑136 (MQ=255) cAGCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCg < 7:194259/136‑1 (MQ=255) cAGCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCg > 2:405408/1‑148 (MQ=255) cAGCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGt > 2:411843/1‑149 (MQ=255) gCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTAc < 7:194326/103‑1 (MQ=255) gCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTAc > 8:194326/1‑103 (MQ=255) ccGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCaa < 1:411843/149‑1 (MQ=255) ccGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCaa < 1:405408/149‑1 (MQ=255) aaTGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAg < 3:168267/135‑1 (MQ=255) aaTGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAg > 4:168267/1‑135 (MQ=255) aaTGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑cc > 7:158625/1‑149 (MQ=255) gTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAg > 4:85250/1‑149 (MQ=255) tGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGt < 3:120209/149‑1 (MQ=255) gTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTtcgt < 5:158273/149‑1 (MQ=255) ttACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTTCGTCGCt > 5:248484/1‑149 (MQ=255) ttACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTTCGTCGCt > 3:55526/1‑149 (MQ=255) ccATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTTCGTCGCTGAAGCg > 4:47544/1‑149 (MQ=255) ccATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTTCGTCGCTGAAGCg > 3:315386/1‑149 (MQ=255) gCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTTCGTCGCTGAAGCGTGCaa < 4:315386/149‑1 (MQ=255) gCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTTCGTCGCTGAAGCGTGCa > 5:281690/1‑148 (MQ=255) aaGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAGCCCCGAGTTCGTCGCTGAAGCGTGCAAAg < 1:4723/149‑1 (MQ=255) aGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTTCGTCGCTGAAGCGTGCAAAGcc < 8:96078/149‑1 (MQ=255) gcTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTTCGTCGCTGAAGCCTGCAAAGCCTTc > 8:27381/1‑149 (MQ=255) tACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTTCGTCGCTGAAGCGTGCAAAGCCTTccc > 6:30252/1‑149 (MQ=255) tACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTTCGTCGCTGAAGCGTGCAAAGCCTTccc > 2:279743/1‑149 (MQ=255) tACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTTCGTCGCTGAAGCGTGCAAAGCCTTccc > 2:307731/1‑149 (MQ=255) aCCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTTCGTCGCTGAAGCGt > 1:145959/1‑135 (MQ=255) aCCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTTCGTCGCTGAAGCGt < 2:145959/135‑1 (MQ=255) aCCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTTCGTCGCTGAAGCGTCCAAAGCATTccct > 4:76284/1‑148 (MQ=255) cccGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTTCGTCGCTGAAGCGTGCAAAGCCTTCCCgg > 4:60299/1‑149 (MQ=255) cccGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTTCGTAGATGAACCGTGCAAAGCCTTCCAgg > 8:367287/1‑149 (MQ=255) ccGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTTCGTCGCTGAAGCGTGCAAAGCCTTCCCggg < 2:131696/149‑1 (MQ=255) ccGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTTCGTCGCTGAAGCGTGCAAAGCCTTCCCggg > 3:323284/1‑149 (MQ=255) gAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTTCGTCGCTGAAGCGTGCAAAGCCTTCCCGGGc > 8:123591/1‑148 (MQ=255) aCCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTTCGTCGCTGAAGCGTGCAAAGCCTTCCCGGGCAAg > 6:414732/1‑149 (MQ=255) ccTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTTCGTCGCTGAAGCGTGCAAAGCCTTCCCGGGCAAgg < 7:367287/149‑1 (MQ=255) ccTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTTCGTCGCTGAAGCGTGCAAAGCCTTCCCGGGCAAgg < 3:60299/149‑1 (MQ=255) ccgCCGATCTGGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTTCGTCGCTGAAGCGTGCAAAGcc > 1:179635/3‑135 (MQ=255) ccgCCGATCTGGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTTCGTCGCTGAAGCGTGCAAAGcc < 2:179635/133‑1 (MQ=255) ccgCCGATCTGGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTTCGTCGCTGAAGCGTGCAAAGCCTTCCCGGGCAAgg > 3:87216/3‑148 (MQ=255) ccgCCGATCTGGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTTCGTCGCTGAAGCGTGCAAAGCCTTCCCGGGCAAgg < 4:87216/146‑1 (MQ=255) cTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTtcgtcg < 8:17371/118‑1 (MQ=255) cTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTtcgtcg > 7:17371/1‑118 (MQ=255) cTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTTCGTCGCTGAAGCGTGCAAAGCCTTCCCGGGCAAGGt > 5:192457/1‑149 (MQ=255) gCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTTCGTCGCTGAAGCGTGCAAAGCCTTCCCGGGCAAGGTCa > 7:365856/1‑149 (MQ=255) gCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTTCGTCGCTGAAGCGTGCAAAGCCTTCCCGGGCAAGGTCa > 4:265124/1‑149 (MQ=255) gCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTTCGTCGCTGAAGCGTGCAAAGCCTTCCCGGGCAAGGTCa > 4:27436/1‑149 (MQ=255) gtCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTTCGTCGCTGAAGCGTGc < 8:275609/122‑1 (MQ=255) gtCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTTCGTCGCTGAAGCGTGc > 7:275609/2‑123 (MQ=255) ccAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTTCGTCGCTGAAGCGTGCAAAGCCTTCCCGGGCAAGGTCAtcgtcg > 4:141792/1‑149 (MQ=255) | ACCCGGTGAGCATGGCCGCCAAGTGGGTCGAGGGTGGCTGCCGCCGCCTGCACCTGGTCGACCTCAACGGCGCCTTCGAAGGCCAGCCGGTCAATGGTGAAGTGGTTACCGCCATCGCCAAGCGCTACCCGAACCTGCCGATCCAGATCGGCGGCGGTA‑‑TC‑CGTTCGCTGGAAACCATCGAACACTACGTCAAGGCTGGCGTCAGCTACGTGATCATCGGCACCAAGGCCGTCAAGCAG‑CCCGAGTTCGTCGCTGAAGCGTGCAAAGCCTTCCCGGGCAAGGTCATCGTCG > NC_002947/351606‑351896 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |