Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,463,701 C→T 7.0% A233V (GCC→GTC)  lolE → lipoprotein releasing system permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,463,7010CT 7.0% 166.2 / 2.3 57A233V (GCC→GTC) lolElipoprotein releasing system permease
Reads supporting (aligned to +/- strand):  ref base C (24/29);  new base T (2/2);  total (26/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

GTGGTCGGCATCTTCAAGGTTGGCGCCGAGCTGGATGGGTCGCAGGCTTATATCCATGTTGCTGATGCAGCCGAGATGCAGCGCTGGGCGCCAGGCCAGGTGCAAGGTGTGCGCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAGGGCAGCCTGTTCAGCGCCATGAAGA‑TGGAAAAGACCATGATCGGCCTGTTGCTGATGATGATCATCGCG  >  NC_002947/2463565‑2463846
                                                                                                                                        |                                                                                                                                                  
gTGGTCGGCATCTTCAAGGTTGGCGCCGAGCTGGATGGGTCGCAGGCTTATATCCATGTTGCTGATGCAGCCGAGATGCAGCGCTGGGCGCCAGGCCAGGTGCAAGGTGTGCTCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGgt                                                                                                                                        <  1:242607/149‑1 (MQ=255)
       gCATCTTCAAGGTTGGCGCCGAGCTGGATGGGTCGCAGGCTTATATCCATGTTGCTGATGCAGCCGAGATGCAGCGCTGGGCGCCAGGCCAGGTGCAAGGTGTGCGCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAg                                                                                                                                 <  7:267706/149‑1 (MQ=255)
              cAAGGTTGGCGCCGAGCTGGATGGGTCGCAGGCTTATATCCATGTTGCTGATGCAGCCGAGATGCAGCGCTGGGCGCCAGGCCAGGTGCAAGGTGTGCGCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGAtt                                                                                                                           <  6:345960/148‑1 (MQ=255)
                           gAGCTGGATGGGTCGCAGGCTTATATCCATGTTGCTGATGCAGCCGAGATGCAGCGCTGGGCGCCAGGCCAGGTGCAAGGTGTGCGCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTgg                                                                                                             <  7:353997/149‑1 (MQ=255)
                            aGCTGGATGGGTCGCAGGCTTATATCCATGTTGCTGATGCAGCCGAGATGCAGCGCTGGGCGCCAGGCCAGGTGCAAGGTGTGCGCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTggg                                                                                                            <  1:26109/149‑1 (MQ=255)
                               tGGATGGGTCGCAGGCTTATATCCATGTTGCTGATGCAGCCGAGATGCAGCGCTGGGCGCCAGGCCAGGTGCAAGGTGTGCGCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGAc                                                                                                         <  4:275180/149‑1 (MQ=255)
                                 gATGGGTCGCAGGCTTATATCCATGTTGCTGATGCAGCCGAGATGCAGCGCTGGGCGCCAGGCCAGGTGCAAGGTGTGCGCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGc                                                                                                       >  7:270035/1‑149 (MQ=255)
                                 gATGGGTCGCAGGCTTATATCCATGTTGCTGATGCAGCCGAGATGCAGCGCTGGGCGCCAGGCCAGGTGCAAGGGGTGCGCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGc                                                                                                       <  7:305345/149‑1 (MQ=255)
                                       tCGCAGGCTTATATCCATGTTGCTGATGCAGCCGAGATGCAGCGCTGGGCGCCAGGCCAGGTGCAAGGTGTGCGCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCg                                                                                                 >  8:229511/1‑149 (MQ=255)
                                               ttATATCCATGTTGCTGATGCAGCCGAGATGCAGCGCTGGGCGCCAGGCCAGGTGCAAGGTGTGCGCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGc                                                                                                       >  1:177369/1‑135 (MQ=255)
                                               ttATATCCATGTTGCTGATGCAGCCGAGATGCAGCGCTGGGCGCCAGGCCAGGTGCAAGGTGTGCGCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGc                                                                                                       <  2:177369/135‑1 (MQ=255)
                                                  tatCCATGTTGCTGATGCAGCCGAGATGCAGCGCTGGGCGCCAGGCCAGGTGCAAGGTGTGCGCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACt                                                                                      >  1:229520/1‑149 (MQ=255)
                                                   atCCATGTTGCTGATGCAGCCGAGATGCAGCGATGGGCGCCAGGCCAGGTGCAAGGTGTGCGCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTg                                                                                     >  4:289792/1‑149 (MQ=255)
                                                              tGATGCAGCCGAGATGCAGCGCTGGGCGCCAGGCCAGGTGCAAGGTGTGCGCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATAccc                                                                          <  8:270035/149‑1 (MQ=255)
                                                              tGATGCAGCCGAGATGCAGCGCTGGGCGCCAGGCCAGGTGCAAGGTGTGCGCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATAcc                                                                           >  2:378235/1‑148 (MQ=255)
                                                               gATGCAGCCGAGATGCAGCGCTGGGCGCCAGGCCAGGTGCAAGGTGTGCGCCTGAAGCTGCACGACCTGTATGTCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATAcc                                                                           >  3:108308/1‑147 (MQ=255)
                                                               gATGCAGCCGAGATGCAGCGCTGGGCGCCAGGCCAGGTGCAAGGTGTGCGCCTGAAGCTGCACGACCTGTATGTCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATAcc                                                                           >  3:114086/1‑147 (MQ=255)
                                                                aTGCAGCCGAGATGCAGCGCTGGGCGCCAGGCCAGGTGCAAGGTGTGCGCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTCTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAg                                                                        >  5:152764/1‑149 (MQ=255)
                                                                    aGCCGAGATGCAGCGCTGGGCGCCAGGCCAGGTGCAAGGTGTGCGCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAggg                                                                      >  5:97039/1‑147 (MQ=255)
                                                                     gCCGAGATGCAGCGCTGGGCGCCAGGCCAGGTGCAAGGTGTGCGCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAGGGCAg                                                                   >  3:186038/1‑149 (MQ=255)
                                                                            tGCAGCGCTGGGCGCCAGGCCAGGTGCAAGGTGTGCGCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATa                                                                             <  6:20242/132‑1 (MQ=255)
                                                                             gCAGCGCTGGGCGCCAGGCCAGGTGCAAGGTGTGCGCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAGGGCAGCCTGtt                                                             >  3:257696/1‑147 (MQ=255)
                                                                             gCAGCGCTGGGCGCCAGGCCAGGTGCAAGGTGTGCGCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAGGGCAGCCTGtt                                                             >  1:310810/1‑147 (MQ=255)
                                                                               aGCGCTGGGCGCCAGGCCAGGTGCAAGGTGTGCGCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAg                                                                        <  7:58399/134‑1 (MQ=255)
                                                                               aGCGCTGGGCGCCAGGCCAGGTGCAAGGTGTGCGCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAg                                                                        >  8:58399/1‑134 (MQ=255)
                                                                                gaccagggCGCCAGGCCAGGTGCAAGGTGTGCGCCTCAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAGGGCAGCCTGTTCa                                                           >  4:226848/6‑146 (MQ=255)
                                                                                gaccagggCGCCAGGCCAGGTGCAAGGTGTGCGCCTCAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAGGGCAGCCTGTTCa                                                           <  3:226848/141‑1 (MQ=255)
                                                                                      ggCGCCAGGCCAGGTGCAAGGTGTGCGCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAGGGCAGCCTGTTCAGCGCCATg                                                   <  7:229511/148‑1 (MQ=255)
                                                                                              gccaggTGCAAGGTGTGCGCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAGGGCAGc                                                                  >  4:315040/1‑125 (MQ=255)
                                                                                              gccaggTGCAAGGTGTGCGCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAGGGCAGc                                                                  <  3:315040/125‑1 (MQ=255)
                                                                                               ccaggTGCAAGGTGTGCGCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGc                                                                                              <  3:27580/96‑1 (MQ=255)
                                                                                               ccaggTGCAAGGTGTGCGCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGc                                                                                              >  4:27580/1‑96 (MQ=255)
                                                                                                 aggTGCAAGGTGTGCGCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAGGGCAGcc                                                                 <  6:120818/123‑1 (MQ=255)
                                                                                                 aggTGCAAGGTGTGCGCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAGGGCAGcc                                                                 >  5:120818/1‑123 (MQ=255)
                                                                                                    tGCAAGGTGTGCGCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAGGGCAGCCTGTTCAGCGCCATGAAGA‑TGGAAAAGAcc                                   <  4:186038/149‑1 (MQ=255)
                                                                                                          gtgtGCGCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAGGGCAGCCTGTTCAGCGCCATGAAGA‑TGGAAAAGACCATGATc                             >  7:113505/1‑149 (MQ=255)
                                                                                                          gtgtGCGCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAGGGCAGCCTGTTCAGCGCCATGAAGA‑TGGAAAAGACCATGATc                             >  7:107822/1‑149 (MQ=255)
                                                                                                          gtgtGCGCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAGGGCAGCCTGTTCAGCGCCATGAAGA‑TGGAAAAGACCATGATc                             <  8:107822/149‑1 (MQ=255)
                                                                                                          gtgtGCGCCNGAAGCTGCACGACNNTNATGNCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAGGGCAGCCTGTTCAGCGCCATGAAGA‑TGGAAAAGACCATGATc                             <  8:113505/149‑1 (MQ=255)
                                                                                                             tgCGCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAGGGCAGCCTGTTCAGCGCCATGAAGA‑TGGAAAAGACCATGATCGGc                          <  6:97039/149‑1 (MQ=255)
                                                                                                               cgcCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACGGGGCCGATGACTGGTCGCATACCCAGGGCAGCCTGTTCTGGGCCATGAAGA‑TGGTAAAGACCATGATCGGCCt                        <  2:310810/149‑1 (MQ=255)
                                                                                                                gcCTGAAGCTGCACGACCTGTATGTCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAGGGCAGCCTGTTCAGCGCCATGAAGA‑TGGAAAAGACCATGATCGGCCTg                       <  4:114086/149‑1 (MQ=255)
                                                                                                                gcCTGAAGCTGCACGACCTGTATGTCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAGGGCAGCCTGTTCAGCGCCATGAAGA‑TGGAAAAGACCATGATCGGCCTg                       <  4:108308/149‑1 (MQ=255)
                                                                                                                    gAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAGGGCAGCCTGTTCAGCGCCATGAAGA‑TGGAAAAGACCATGATCGGCCTGTTGc                   >  4:199628/1‑149 (MQ=255)
                                                                                                                     aaGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAgg                                                                       <  3:339314/97‑1 (MQ=255)
                                                                                                                     aaGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAgg                                                                       >  4:339314/1‑97 (MQ=255)
                                                                                                                     aaGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAGGGCAGCCTGTTCAGCGCCATGAAGA‑TGGAAAAGACCATGATCGGCCTGTTGCt                  <  2:229520/149‑1 (MQ=255)
                                                                                                                        cTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAGGGCAGCCTGTTCAGCGCCATGAAGA‑TGGa                                          >  6:389956/1‑122 (MQ=255)
                                                                                                                        cTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAGGGCAGCCTGTTCAGCGCCATGAAGA‑TGGa                                          <  5:389956/122‑1 (MQ=255)
                                                                                                                           cACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAGGGCAGCCTGTTCAGCGCCATGAAGA‑TGGAAAAGACCATGATCGGCCTGTTGCtga                <  3:236730/145‑1 (MQ=255)
                                                                                                                           cACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAGGGCAGCCTGTTCAGCGCCATGAAGA‑TGGAAAAGACCATGATCGGCCTGTTGCtga                >  4:236730/1‑145 (MQ=255)
                                                                                                                             cGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAGGGCAGCCTGTTCAGCGCCATGAAGA‑TGGAAAAGAACATGATCGGCCTGTTGCtgatgatga          <  3:289792/149‑1 (MQ=255)
                                                                                                                               aCCTGTATGCCGCTGCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAGGGCAGCCTGTTCAGCGCCATGAAGA‑TGGAAAAGACCATGATCGGCCTGTTGCTGATGATGatc        <  4:316020/149‑1 (MQ=255)
                                                                                                                               aCCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAGGGCAGCCTGTTCAGCGCCATGAAGA‑TGGAAAAGACCATGATCGGCCTGTTGCTgatgatgat         >  2:41307/1‑148 (MQ=255)
                                                                                                                                 cTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAGGGCAGCCTGTTCAGCGCCATGAAGATTGGAAAAGACCATGATCGGCCTGTTGCtgatga             <  1:262798/143‑1 (MQ=255)
                                                                                                                                 cTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAGGGCAGCCTGTTCAGCGCCATGAAGA‑TGGAAAAGACCATGATCGGCCTGTTGCtgatga             >  2:262798/1‑142 (MQ=255)
                                                                                                                                      tGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAGGGCAGCCTGTTCAGCGCCTTGAAGA‑TGGAAAAGACCATGATCGGCCTGTTGCTGATGATTATCATcgcg  <  6:152764/148‑1 (MQ=255)
                                                                                                                                       gCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAGGGCAGCCTGTTCAGCGCCATGAAGA‑TGGAAAAGACCa                                  <  6:390131/115‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                                  
GTGGTCGGCATCTTCAAGGTTGGCGCCGAGCTGGATGGGTCGCAGGCTTATATCCATGTTGCTGATGCAGCCGAGATGCAGCGCTGGGCGCCAGGCCAGGTGCAAGGTGTGCGCCTGAAGCTGCACGACCTGTATGCCGCTCCGCAGGTGTCCAAGGCGATTGCTGCCGGGCTTGGGGACGCCTACCGGGCCGATGACTGGTCGCATACCCAGGGCAGCCTGTTCAGCGCCATGAAGA‑TGGAAAAGACCATGATCGGCCTGTTGCTGATGATGATCATCGCG  >  NC_002947/2463565‑2463846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑