Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,469,851 A→T 7.8% intergenic (+20/+15) vacJ → / ← PP_2164 lipoprotein VacJ/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,469,8510AT 7.8% 192.4 / 5.2 64intergenic (+20/+15)vacJ/PP_2164lipoprotein VacJ/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (25/34);  new base T (3/2);  total (28/36)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

ACACCCGCGCCAGCCTGCTCTCGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGATCCGTACCTCGAT  >  NC_002947/2469704‑2469994
                                                                                                                                                   |                                                                                                                                               
acacCCGCGCCAGCCTGCTCTCGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCa                                                                                                                                                 >  2:183656/1‑148 (MQ=255)
   cccGCGCCAGCCTGCTCTCGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGc                                                                                                                                             >  2:342270/1‑149 (MQ=255)
   cccGCGCCAGCCTGATCTCGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGc                                                                                                                                             <  2:310610/149‑1 (MQ=255)
             ccTGCTCTCGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGt                                                                                                                                   >  5:185345/1‑149 (MQ=255)
                    tcGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCaac                                                                                                                              <  2:37094/147‑2 (MQ=255)
                    tcGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAAt                                                                                                                              <  6:185345/147‑1 (MQ=255)
                      ggCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGc                                                                                                                            <  2:317023/147‑1 (MQ=255)
                         cGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCTAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGc                                                                                                                       <  4:225538/149‑1 (MQ=255)
                         cGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGc                                                                                                                       <  5:408246/149‑1 (MQ=255)
                          gAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGcc                                                                                                                      <  7:151700/149‑1 (MQ=255)
                           aaaaGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCa                                                                                                                     <  3:125971/149‑1 (MQ=255)
                                               aaGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTc                                                                                                 >  7:87132/1‑149 (MQ=255)
                                               aaGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTc                                                                                                 >  5:38605/1‑149 (MQ=255)
                                                 gTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGTTGGCGAGGTGGAAGACGACTTGTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCATAACTtgtg                                                                                               >  8:204730/1‑149 (MQ=255)
                                                 gTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAACGCGGCCTCGTGTTCAATGCATGTCCAGAATCCGCAGCCCGAACTTctg                                                                                               >  8:204729/1‑149 (MQ=255)
                                                 gTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCAAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTctg                                                                                               <  1:356771/149‑1 (MQ=255)
                                                    cATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGTGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTctgct                                                                                             <  1:295222/148‑1 (MQ=255)
                                                    cATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTctgctg                                                                                            <  5:111730/149‑1 (MQ=255)
                                                      tCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACTACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTgcc                                                                                          >  6:399807/1‑149 (MQ=255)
                                                         tCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACTACATCTGATCTGTGAACCTTGGGCCGCAAAGCGGACTCGGGTTCAAAGCATCGCCAGAATCCGCAGCCCGAACTTCTGCTgcacgc                                                                                       >  4:10297/1‑149 (MQ=255)
                                                         tCATTCGCAATGACTACCTGCAAAACCGCGAGTTAAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCACGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTgcccgc                                                                                       >  3:156592/1‑149 (MQ=255)
                                                                cAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTg                                                                                >  7:99100/1‑149 (MQ=255)
                                                                   tGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTc                                                                             >  6:401133/1‑149 (MQ=255)
                                                                        aCCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCa                                                                         >  8:148313/1‑148 (MQ=255)
                                                                         gttGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAAt                                                                       <  4:34740/147‑1 (MQ=255)
                                                                         ccTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCgg                                                                           <  2:367119/145‑1 (MQ=255)
                                                                         ccTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCgg                                                                           >  1:367119/1‑145 (MQ=255)
                                                                         ccTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCaa                                                                        <  1:354498/148‑1 (MQ=255)
                                                                         ccTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAAt                                                                       <  5:328950/149‑1 (MQ=255)
                                                                            gCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATGTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCa                                                                    >  1:312982/1‑149 (MQ=255)
                                                                             cAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCa                                                                    <  6:38605/148‑1 (MQ=255)
                                                                              aaaaCCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATa                                                                  <  5:401133/149‑1 (MQ=255)
                                                                               aaaCCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATAc                                                                 <  2:9545/149‑1 (MQ=255)
                                                                                      gAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTTACCCATGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCg                                                          <  1:342270/149‑1 (MQ=255)
                                                                                         ttCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTg                                                       <  8:87132/149‑1 (MQ=255)
                                                                                            aaGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTg                                                    >  8:249630/1‑149 (MQ=255)
                                                                                             aGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTgg                                                   <  2:312982/149‑1 (MQ=255)
                                                                                              ggTGAAGGATGGCGAGGTCGAAGACGACTTCAGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGTGATCAATGAAAGGCCAGAATCAGCAGCGCGAACTTCTGCTGCCCGCCCGGCt                                                                                 >  6:187524/1‑118 (MQ=255)
                                                                                                 gAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATg                                               <  3:10297/149‑1 (MQ=255)
                                                                                                   aGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCa                                             <  5:98768/149‑1 (MQ=255)
                                                                                                      aTGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGcc                                          <  7:148313/149‑1 (MQ=255)
                                                                                                         gagtggTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCGCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGcccc                                        <  7:204729/144‑1 (MQ=255)
                                                                                                         gCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGTGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGcccc                                        <  5:399807/148‑1 (MQ=255)
                                                                                                         gCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCt                                       >  7:97342/1‑149 (MQ=255)
                                                                                                          cGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACtt                                                                                                  >  1:338121/1‑89 (MQ=255)
                                                                                                          cGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACtt                                                                                                  <  2:338121/89‑1 (MQ=255)
                                                                                                                 gAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGcc                               >  8:377949/1‑149 (MQ=255)
                                                                                                                 gAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGcc                               >  6:399480/1‑149 (MQ=255)
                                                                                                                 gAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGcc                               <  1:183656/149‑1 (MQ=255)
                                                                                                                   aGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCg                              >  7:57275/1‑148 (MQ=255)
                                                                                                                      cgacTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGAt                           <  8:97342/148‑1 (MQ=255)
                                                                                                                          ttCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACg                      >  6:71065/1‑149 (MQ=255)
                                                                                                                               tgcattgAACCCGAGGCCGCTTTGCGGCCCCAGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTc                 >  3:309251/6‑149 (MQ=38)
                                                                                                                                  attgAACCCGAGGCCGCTTTGCGGCCCCAGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCg                                                          <  2:133834/103‑1 (MQ=37)
                                                                                                                                  attgAACCCGAGGCCGCTTTGCGGCCCCAGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCg                                                          >  1:133834/3‑105 (MQ=37)
                                                                                                                                    tgAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATAc                                                                 <  2:345852/96‑1 (MQ=255)
                                                                                                                                    tgAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATAc                                                                 >  1:345852/1‑96 (MQ=255)
                                                                                                                                    tgAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGATc             >  8:329094/1‑148 (MQ=255)
                                                                                                                                      aaCCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATCGACTCCGTCCATCCgg          <  7:377949/149‑2 (MQ=255)
                                                                                                                                      aaCCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCACGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGATCCGt          <  5:399480/149‑1 (MQ=255)
                                                                                                                                      aaCCCGAGGCCGCTTTGCGGCCCCAGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGa               <  2:70530/144‑1 (MQ=255)
                                                                                                                                      aaCCCGAGGCCGCTTTGCGGCCCCAGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGa               >  1:70530/1‑144 (MQ=255)
                                                                                                                                              gCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGATCCGTACCTCGAt  <  4:156592/149‑1 (MQ=255)
                                                                                                                                                  cAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATg                          >  4:358765/1‑121 (MQ=255)
                                                                                                                                                  cAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATg                          <  3:358765/121‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
ACACCCGCGCCAGCCTGCTCTCGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGATCCGTACCTCGAT  >  NC_002947/2469704‑2469994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑