Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,469,860 T→C 9.9% intergenic (+29/+6) vacJ → / ← PP_2164 lipoprotein VacJ/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,469,8600TC 9.9% 152.2 / 6.8 63intergenic (+29/+6)vacJ/PP_2164lipoprotein VacJ/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (23/32);  new base C (3/3);  total (27/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

CCTGCTCTCGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGATCCGTACCTCGATCTGA  >  NC_002947/2469717‑2469998
                                                                                                                                               |                                                                                                                                          
ccTGCTCTCGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGt                                                                                                                                       >  5:185345/1‑149 (MQ=255)
       tcGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCaac                                                                                                                                  <  2:37094/147‑2 (MQ=255)
       tcGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAAt                                                                                                                                  <  6:185345/147‑1 (MQ=255)
         ggCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGc                                                                                                                                <  2:317023/147‑1 (MQ=255)
            cGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCTAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGc                                                                                                                           <  4:225538/149‑1 (MQ=255)
            cGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGc                                                                                                                           <  5:408246/149‑1 (MQ=255)
             gAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGcc                                                                                                                          <  7:151700/149‑1 (MQ=255)
              aaaaGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCa                                                                                                                         <  3:125971/149‑1 (MQ=255)
                                  aaGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTc                                                                                                     >  5:38605/1‑149 (MQ=255)
                                  aaGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTc                                                                                                     >  7:87132/1‑149 (MQ=255)
                                    gTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGTTGGCGAGGTGGAAGACGACTTGTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCATAACTtgtg                                                                                                   >  8:204730/1‑149 (MQ=255)
                                    gTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAACGCGGCCTCGTGTTCAATGCATGTCCAGAATCCGCAGCCCGAACTTctg                                                                                                   >  8:204729/1‑149 (MQ=255)
                                    gTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCAAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTctg                                                                                                   <  1:356771/149‑1 (MQ=255)
                                       cATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGTGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTctgct                                                                                                 <  1:295222/148‑1 (MQ=255)
                                       cATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTctgctg                                                                                                <  5:111730/149‑1 (MQ=255)
                                         tCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACTACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTgcc                                                                                              >  6:399807/1‑149 (MQ=255)
                                            tCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACTACATCTGATCTGTGAACCTTGGGCCGCAAAGCGGACTCGGGTTCAAAGCATCGCCAGAATCCGCAGCCCGAACTTCTGCTgcacgc                                                                                           >  4:10297/1‑149 (MQ=255)
                                            tCATTCGCAATGACTACCTGCAAAACCGCGAGTTAAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCACGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTgcccgc                                                                                           >  3:156592/1‑149 (MQ=255)
                                                   cAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTg                                                                                    >  7:99100/1‑149 (MQ=255)
                                                      tGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTc                                                                                 >  6:401133/1‑149 (MQ=255)
                                                           aCCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCa                                                                             >  8:148313/1‑148 (MQ=255)
                                                            gttGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAAt                                                                           <  4:34740/147‑1 (MQ=255)
                                                            ccTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCgg                                                                               <  2:367119/145‑1 (MQ=255)
                                                            ccTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCgg                                                                               >  1:367119/1‑145 (MQ=255)
                                                            ccTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCaa                                                                            <  1:354498/148‑1 (MQ=255)
                                                            ccTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAAt                                                                           <  5:328950/149‑1 (MQ=255)
                                                               gCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATGTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCa                                                                        >  1:312982/1‑149 (MQ=255)
                                                                cAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCa                                                                        <  6:38605/148‑1 (MQ=255)
                                                                 aaaaCCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATa                                                                      <  5:401133/149‑1 (MQ=255)
                                                                  aaaCCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATAc                                                                     <  2:9545/149‑1 (MQ=255)
                                                                         gAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTTACCCATGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCg                                                              <  1:342270/149‑1 (MQ=255)
                                                                            ttCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTg                                                           <  8:87132/149‑1 (MQ=255)
                                                                               aaGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTg                                                        >  8:249630/1‑149 (MQ=255)
                                                                                aGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTgg                                                       <  2:312982/149‑1 (MQ=255)
                                                                                 ggTGAAGGATGGCGAGGTCGAAGACGACTTCAGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGTGATCAATGAAAGGCCAGAATCAGCAGCGCGAACTTCTGCTGCCCGCCCGGCt                                                                                     >  6:187524/1‑118 (MQ=255)
                                                                                    gAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATg                                                   <  3:10297/149‑1 (MQ=255)
                                                                                      aGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCa                                                 <  5:98768/149‑1 (MQ=255)
                                                                                         aTGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGcc                                              <  7:148313/149‑1 (MQ=255)
                                                                                            gagtggTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCGCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGcccc                                            <  7:204729/144‑1 (MQ=255)
                                                                                            gCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGTGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGcccc                                            <  5:399807/148‑1 (MQ=255)
                                                                                            gCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCt                                           >  7:97342/1‑149 (MQ=255)
                                                                                             cGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACtt                                                                                                      <  2:338121/89‑1 (MQ=255)
                                                                                             cGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACtt                                                                                                      >  1:338121/1‑89 (MQ=255)
                                                                                                    gAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGcc                                   >  8:377949/1‑149 (MQ=255)
                                                                                                    gAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGcc                                   <  1:183656/149‑1 (MQ=255)
                                                                                                    gAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGcc                                   >  6:399480/1‑149 (MQ=255)
                                                                                                      aGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCg                                  >  7:57275/1‑148 (MQ=255)
                                                                                                         cgacTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGAt                               <  8:97342/148‑1 (MQ=255)
                                                                                                             ttCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACg                          >  6:71065/1‑149 (MQ=255)
                                                                                                                  tgcattgAACCCGAGGCCGCTTTGCGGCCCCAGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTc                     >  3:309251/6‑149 (MQ=38)
                                                                                                                     attgAACCCGAGGCCGCTTTGCGGCCCCAGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCg                                                              <  2:133834/103‑1 (MQ=37)
                                                                                                                     attgAACCCGAGGCCGCTTTGCGGCCCCAGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCg                                                              >  1:133834/3‑105 (MQ=37)
                                                                                                                       tgAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATAc                                                                     <  2:345852/96‑1 (MQ=255)
                                                                                                                       tgAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATAc                                                                     >  1:345852/1‑96 (MQ=255)
                                                                                                                       tgAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGATc                 >  8:329094/1‑148 (MQ=255)
                                                                                                                         aaCCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATCGACTCCGTCCATCCgg              <  7:377949/149‑2 (MQ=255)
                                                                                                                         aaCCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCACGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGATCCGt              <  5:399480/149‑1 (MQ=255)
                                                                                                                         aaCCCGAGGCCGCTTTGCGGCCCCAGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGa                   <  2:70530/144‑1 (MQ=255)
                                                                                                                         aaCCCGAGGCCGCTTTGCGGCCCCAGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGa                   >  1:70530/1‑144 (MQ=255)
                                                                                                                                 gCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGATCCGTACCTCGAt      <  4:156592/149‑1 (MQ=255)
                                                                                                                                     ctttgCGGCCCCAGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGATCCGTACCTCGATCTGa  <  6:76528/145‑1 (MQ=255)
                                                                                                                                     cAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATg                              >  4:358765/1‑121 (MQ=255)
                                                                                                                                     cAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATg                              <  3:358765/121‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                          
CCTGCTCTCGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGATCCGTACCTCGATCTGA  >  NC_002947/2469717‑2469998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑