Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 353,067 A→G 100% intergenic (+34/+84) hisF → / ← cbcV imidazole glycerol phosphate synthase subunit HisF/choline/betaine/carnitine ABC transporter ATP binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947353,0670AG100.0% 192.7 / NA 57intergenic (+34/+84)hisF/cbcVimidazole glycerol phosphate synthase subunit HisF/choline/betaine/carnitine ABC transporter ATP binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/30);  total (27/30)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCAGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGT‑GGTAAAGCTCGGTATCCCCGA  >  NC_002947/352929‑353200
                                                                                                                                          |                                                                                                                                       
gggCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCg                                                                                                                               >  4:246774/1‑149 (MQ=255)
    aTGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑cc                                                                                                                          <  5:409735/149‑1 (MQ=255)
    aTGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑cc                                                                                                                          >  2:337863/1‑149 (MQ=255)
       ccAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCca                                                                                                                        <  3:246774/148‑1 (MQ=255)
                   tGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCt                                                                                                              >  8:225571/1‑146 (MQ=255)
                    ggcggcCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACACGTTTGCTgag                                                                                                           >  3:256685/1‑148 (MQ=255)
                             cATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGc                                                                                                                                  <  6:392695/117‑1 (MQ=255)
                             cATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGc                                                                                                                                  >  5:392695/1‑117 (MQ=255)
                                 ttCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCca                                                                                                                        <  4:214439/122‑1 (MQ=255)
                                 ttCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCca                                                                                                                        >  3:214439/1‑122 (MQ=255)
                                   ccACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGa                                                                                               <  5:372991/145‑1 (MQ=255)
                                   ccACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGa                                                                                               <  5:135811/145‑1 (MQ=255)
                                   ccACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAg                                                                                              <  5:129786/146‑1 (MQ=255)
                                       tttGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑c                                                                                                                           >  2:28644/1‑113 (MQ=255)
                                       tttGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑c                                                                                                                           <  1:28644/113‑1 (MQ=255)
                                         tGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGt                                                                                       <  3:350349/147‑1 (MQ=255)
                                           gCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCgg                                                                                           <  7:724/141‑1 (MQ=255)
                                           gCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCgg                                                                                           >  8:724/1‑141 (MQ=255)
                                           gCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGcc                                                                                    <  1:125893/148‑1 (MQ=255)
                                             gAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGCTCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGc                                                                                             >  5:297249/1‑137 (MQ=255)
                                             gAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGc                                                                                             <  6:297249/137‑1 (MQ=255)
                                             gAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCgg                                                                                           >  2:303330/1‑139 (MQ=255)
                                             gAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCgg                                                                                           <  1:303330/139‑1 (MQ=255)
                                             gAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCcgc                                                                                 >  6:2676/1‑149 (MQ=255)
                                                tACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCAAGCCCGCT‑‑CCACAGGGTTGCTGAGATCTCTGTTGGAGCCGGGGTGCCCGCGAAg                                                                             >  3:235849/1‑149 (MQ=38)
                                                  cacaGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAgg                                                                            >  6:127850/1‑149 (MQ=255)
                                                              ggCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCgg                                                                <  3:62715/149‑1 (MQ=255)
                                                                ccAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAg                                                                                                                     >  6:275001/1‑94 (MQ=255)
                                                                 cAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGccc                                                             >  5:219532/1‑149 (MQ=255)
                                                                  aaGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCCCGAAGGGCCGCGAAGAGGccc                                                             >  4:265429/1‑148 (MQ=255)
                                                                      ccTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGACCGCGAAGCGGCCCCAAtt                                                        <  1:28766/149‑1 (MQ=255)
                                                                       cTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACCCCCCCA‑CCCACAGGTTTGCTGAGATCTGTGTGGGAGCGGGCGTGGCCGCGCAGGGCCCCGCAGCGGCCCCAATTg                                                       >  1:223199/1‑149 (MQ=38)
                                                                          cttGGCTTGGCGCGGGTTCGGCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTTAGATCTCTGTGGTAGGGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCtt                                                    <  2:223199/147‑1 (MQ=38)
                                                                                 tCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCCCA‑CCCACAGGTTTGCTGGGAACGATGTGGGATCCGGCGTCCCCCCGAAGGGCCGCGAAGCCGCCCCCAATGGTTCAACCGt                                             >  4:398176/1‑149 (MQ=25)
                                                                                  cgcgcgGGATCGGCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACTGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGt                                             <  5:202641/148‑1 (MQ=255)
                                                                                   gcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTgg                                           <  7:225571/149‑1 (MQ=255)
                                                                                     gcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGa                                                                                               <  1:56259/95‑1 (MQ=255)
                                                                                     gcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGa                                                                                               >  2:56259/1‑95 (MQ=255)
                                                                                          aTCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATctct                                                                                                     <  5:297527/84‑1 (MQ=255)
                                                                                          aTCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATctct                                                                                                     >  6:297527/1‑84 (MQ=255)
                                                                                             gtcgtTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCg                                  <  5:2676/148‑1 (MQ=255)
                                                                                             gtcgtTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGa                                 <  6:180479/149‑1 (MQ=255)
                                                                                                 ttCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGagcag                             >  5:106683/1‑149 (MQ=255)
                                                                                                         tCCATTTACGGGGGGTTCTTTTGAGCCCTTCGCGGTCACGCCCGGTCCCCACGAGTTTGCTTTTTTCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGT‑g                      <  1:337863/148‑1 (MQ=21)
                                                                                                           cATTGACCGGGGCTGCTTTGCAGCCGTTCGCGGGCACGCCGGCT‑CCCACAGGTTTGCTGAGTTCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGag                                <  7:155248/136‑1 (MQ=255)
                                                                                                           cATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGT‑GGTa                   <  5:127850/148‑1 (MQ=255)
                                                                                                            aTTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGc                                    >  8:262683/1‑131 (MQ=255)
                                                                                                            aTTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGc                                    <  7:262683/131‑1 (MQ=255)
                                                                                                                aCCGGGGCTGCTTTGCAGCCCTTCGCGGGCACCCCCCCG‑ACCACACGTTTGCTGTGCTCTCTGTGGGCGCGGGCGGGCCCCCGCACGGCCGCGAAGCGGCCCCAATTGCGTCAACCGGGGTTGGTGGCGCGCAGCGCgg‑gggtaaac               >  6:228154/1‑141 (MQ=255)
                                                                                                                 ccGGGGCTGCTTTGCAGCCCTTCGCGGGCACCCCCCCA‑CCCCCAGGTTTTGGGAGATATATGTTGGAGCGGGCGTGGCCGCGAAAGGCCGCCAAGCGGGCCCAATTTGGTAAACCCTTGGTATTGGCGAGGAACGCGTGGGTAAAgtag             >  4:219349/1‑146 (MQ=255)
                                                                                                                  cGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGT‑GGTAAAGCTCgg           >  4:272908/1‑149 (MQ=255)
                                                                                                                   ggggCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGT‑GGTAAAGCTCGGt          <  6:219532/149‑1 (MQ=255)
                                                                                                                       ctgctTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAgcgc                          >  3:111986/1‑130 (MQ=255)
                                                                                                                       ctgctTTGCAGCCCTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAgcgc                          <  4:111986/130‑1 (MQ=255)
                                                                                                                          gttggCAGCCGTTCGCGGGCATGCCCGCT‑‑CCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGT‑GGTAAAGCTCGGTATCCCCGa  <  4:235849/145‑1 (MQ=38)
                                                                                                                                  cccTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGT‑g                      >  2:279753/1‑122 (MQ=255)
                                                                                                                                  cccTTCGCGGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGT‑g                      <  1:279753/122‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                       
GGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCAGGCACGCCCGCT‑CCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGT‑GGTAAAGCTCGGTATCCCCGA  >  NC_002947/352929‑353200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑