Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,508,790 T→C 6.3% M109V (ATG→GTG)  PP_2203 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,508,7900TC 6.3% 176.5 / 2.6 66M109V (ATG→GTG) PP_2203hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (37/23);  new base C (2/2);  total (40/26)
Fisher's exact test for biased strand distribution p-value = 6.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.65e-01

GGCACCAAAGGCGCTGAGAAACAGCGCCCCTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACG  >  NC_002947/2508645‑2508938
                                                                                                                                                 |                                                                                                                                                    
ggcACCAAAGGCGCTGAGAAACAGCGCCCCTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGcc                                                                                                                                                   >  5:250804/1‑149 (MQ=255)
ggcACCAAAGGCGCTGAGAAACAGCGCCCCTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGcc                                                                                                                                                   <  5:67943/149‑1 (MQ=255)
  cACCAAAGGCGCTGAGAAACAGCGCCCCTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCATGCCCATGGGCTCGCCCg                                                                                                                                                 <  1:403813/149‑1 (MQ=255)
   aCCAAAGGCGCTGAGAAACAGCGCCCCTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCgg                                                                                                                                                >  6:315425/1‑149 (MQ=255)
     cAAAGGCGCTGAGAAACAGCGCCCCTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCa                                                                                                                                              >  8:375716/1‑149 (MQ=255)
            gcTGAGAAACAGCGCCCCTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAg                                                                                                                                       <  7:375716/149‑1 (MQ=255)
              tGAGAAACAGCGCCCCTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGc                                                                                                                                     >  2:198379/1‑149 (MQ=255)
              tGAGAAACAGCGCCCCTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCATGCCCATGGGCTCGccc                                                                                                                                                  >  1:30138/1‑136 (MQ=255)
              tGAGAAACAGCGCCCCTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCATGCCCATGGGCTCGccc                                                                                                                                                  <  2:30138/136‑1 (MQ=255)
                agaAACAGCGCCCCTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGccc                                                                                                                                   >  5:135453/1‑149 (MQ=255)
                 gaAACAGCGCCCCTCGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCGTGGGTCACCCCGTTGACGTGGTAGGCATCCTTGTGGTCACCCTTCTCCTTGCCGGGCGAGCCCTTGGGCATGCCCGGCACCGCCAg                                                                                                                                       <  5:369594/144‑1 (MQ=255)
                 gaAACAGCGCCCCTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAg                                                                                                                                       >  6:369594/1‑144 (MQ=255)
                   acccgcgcCCCTTGGCGGAGCATCTGTAGTTGGCCCCCCCGGTGGCCTGGCCATCCGGGGGCCCCCCTATTCCGTGGTTGGCATCCTTGTGGCCCCCCATCTCCATGCCGGGCGCCCCCTTGGGCATGCCCGGCACCGCCAGGCCCTTg                                                                                                                                <  2:370189/145‑1 (MQ=255)
                   aaCAGCGCCCCTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCtt                                                                                                                                 >  6:172870/1‑148 (MQ=255)
                    aCAGCGCCCCTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCA‑CCCGATGGCCTGGTAGGCATGCGTGTTGTGACCCATATCCATTCCGGGCGAGCCCATGGGCATTCCCGGCACCGCCAGGcccgttag                                                                                                                              >  1:224848/1‑144 (MQ=255)
                           cccTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGtt                                                                                                                        >  2:83312/1‑149 (MQ=255)
                            ccTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTg                                                                                                                       >  2:375456/1‑149 (MQ=255)
                             cTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGc                                                                                                                      >  2:78538/1‑149 (MQ=255)
                                   tGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTgg                                                                                                                >  7:95411/1‑149 (MQ=255)
                                     aGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGGCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGc                                                                                                               <  2:129225/148‑1 (MQ=255)
                                       cATCAGTAGTTGGCGACCCCGGGGTCCTGGCCACCCGGGGCCCCCCCGATGACCTGGTAGGCACCCTTGGGGTCACCCATCACCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCcag                                                                                                            <  5:315425/149‑1 (MQ=255)
                                         tCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCcag                                                                                                            >  7:236443/1‑147 (MQ=255)
                                           agtagtTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGTTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAgg                                                                                                        <  8:95411/149‑1 (MQ=255)
                                             tagtTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAg                                                                                                                              >  1:222911/1‑125 (MQ=255)
                                                   gCGACCAGGGTGTCCTGGCCATCCTGGGTCACCCCGATGACGTGTTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCAc                                                                                                   <  8:92702/146‑1 (MQ=255)
                                                   gCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCAc                                                                                                   >  7:92702/1‑146 (MQ=255)
                                                    cGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGCGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGc                                                                                               <  1:241129/149‑1 (MQ=255)
                                                     gACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCt                                                                                              >  4:179193/1‑149 (MQ=255)
                                                          cGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCTCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTcggcc                                                                                         <  1:78538/149‑1 (MQ=255)
                                                            gtgtCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTcggccgg                                                                                       <  7:287440/149‑1 (MQ=255)
                                                            gtgtCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTcggccgg                                                                                       >  8:287440/1‑149 (MQ=255)
                                                                 cTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGt                                                                                  >  3:282004/1‑149 (MQ=255)
                                                                    gCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTg                                                                                 <  1:83312/147‑1 (MQ=255)
                                                                      cATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCt                                                                             >  5:129548/1‑149 (MQ=255)
                                                                          cTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTcga                                                                         >  4:120156/1‑149 (MQ=255)
                                                                            gggTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTcg                                                                                            <  6:297180/128‑1 (MQ=255)
                                                                            gggTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTcg                                                                                            >  5:297180/1‑128 (MQ=255)
                                                                             ggTCACCCCGATGACCTGGTAGGCATCCATGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTcgacg                                                                       >  1:23328/1‑148 (MQ=255)
                                                                               tCACCCCGATGACGTGGTAGGCATCATTGTGGTCACCAATCTCCATGCCGGGCTAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAAc                                                                    <  6:135453/149‑1 (MQ=255)
                                                                                     cGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTgccg                                                              >  2:131687/1‑149 (MQ=255)
                                                                                      gATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTgccgc                                                             >  3:162121/1‑149 (MQ=255)
                                                                                         gACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGa                                                          >  7:333429/1‑149 (MQ=255)
                                                                                                        cTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTgg                                            <  6:129548/148‑1 (MQ=255)
                                                                                                         ttGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCa                                          >  3:154118/1‑149 (MQ=255)
                                                                                                            ggtagaCCCATCTCCATGCCGGGCGAGCCCATCGGCAAGCCCGGCACCGCGAGGCCCGAGAGCTCGATGCGTTTCGCCAGCAGGCGCACCAGCTCGGCCGGCGCGGGGACTTCGTCGAACAAGCCGCAGATCCCCCCGGGGTGGCacca                                       >  6:40506/6‑147 (MQ=255)
                                                                                                                 aCCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCaa                                  <  3:120156/149‑1 (MQ=255)
                                                                                                                 aCCCATCACCGGGCGGGGCGCCCCCTGGGGCCGGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCaa                                  <  1:198379/149‑1 (MQ=255)
                                                                                                                            tGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTgccgc                                                             <  8:99006/111‑1 (MQ=255)
                                                                                                                            tGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTgccgc                                                             >  7:99006/1‑111 (MQ=255)
                                                                                                                            tGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGgcgc                        >  2:45934/1‑148 (MQ=255)
                                                                                                                              ccGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCacgacg                                     >  3:144146/1‑133 (MQ=255)
                                                                                                                              ccGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGGCGCCAc                     >  4:145808/1‑149 (MQ=255)
                                                                                                                              ccGGGCATGCCCATGGGCTCGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGGCGCCAc                     >  4:246884/1‑149 (MQ=255)
                                                                                                                                gggcatgCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGGCGCCACGc                   >  7:388914/7‑149 (MQ=255)
                                                                                                                                gggCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGc                               >  1:107793/1‑137 (MQ=255)
                                                                                                                                gggCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGc                               <  2:107793/137‑1 (MQ=255)
                                                                                                                                gggCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGgc                          <  7:92275/142‑1 (MQ=255)
                                                                                                                                gggCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGgc                          >  8:92275/1‑142 (MQ=255)
                                                                                                                                gggCGAGCCCATGCGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGACGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGGCGCCACGc                   >  6:392272/1‑149 (MQ=255)
                                                                                                                                      gCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGc             >  7:367986/1‑149 (MQ=255)
                                                                                                                                         cATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGtt          >  3:275027/1‑149 (MQ=255)
                                                                                                                                         cATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGtt          <  2:23328/149‑1 (MQ=255)
                                                                                                                                            gggCATGCCGGGCACCGCCAGGCCCTTGAGGGCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCt       <  7:70297/149‑1 (MQ=255)
                                                                                                                                               cATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGa    <  5:77367/149‑1 (MQ=255)
                                                                                                                                                aTGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGCGTGGCGCCACGCCTTGCGTCGTGCCACAcc                  <  3:346083/134‑1 (MQ=38)
                                                                                                                                                aTGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGCGTGGCGCCACGCCTTGCGTCGTGCCACAcc                  >  4:346083/1‑134 (MQ=38)
                                                                                                                                                 tGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACg  <  5:172870/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GGCACCAAAGGCGCTGAGAAACAGCGCCCCTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACG  >  NC_002947/2508645‑2508938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑