Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,527,616 G→A 7.2% A220T (GCC→ACC)  acd → acyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,527,6160GA 7.2% 164.1 / 2.2 55A220T (GCC→ACC) acdacyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (27/24);  new base A (2/2);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

ACCCGGAGGCCGGCAAGCGTGGCATCAGCGCCTTCATCGTGCCGACCGATTCGCCGGGCTACCAGGTAGCGCGGGTGGAGGACAAACTCGGCCAGCACGCCTCCGACACCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCTTGAAGGCGGCCGTATCGGCATCGCCTCGCAAGCGGTGGGTATGGCCCGCGCGGCGTTCGAAGTGGCGCGGGACTATGCCAACGAGCGCCAGA  >  NC_002947/2527470‑2527760
                                                                                                                                                  |                                                                                                                                                
aCCCGGAGGCCGGCAAGCGTGGCATCAGCGCCTTCATCGTGCCGACCGATTCGCCGGGCTACCAGGTAGCACGGGTGGAGGACAAACTCGGCCAGCACGCCTCCGACACCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGc                                                                                                                                                 >  1:224193/1‑148 (MQ=255)
         ccGGCAAGCGTGGCATCAGCGCCTTCATCGTGCCGACCGATTCGCCGGGCTACCAGGTAGCGCGGGTGGAGGACAAACTCGGCCAGCACGCCTCCGACACCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTg                                                                                                                                       <  5:79179/149‑1 (MQ=255)
             cAAGCGTGGCATCAGCGCCTTCATCGTGCCGACCGATTCGCCGGGCTACCAGGTAGCGCGGGTGGAGGACAAACTCGGCCAGCACGCCTCCGACACCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTggggg                                                                                                                                   >  2:192844/1‑149 (MQ=255)
                  gTGGCATCAGCGCCTTCATCGTGCCGACCGATTCGCCGGGCTACCAGGTAGCGCGGGTGGAGGACAAACTCGGCCAGCACGCCTCCGACACCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAg                                                                                                                              <  3:211880/149‑1 (MQ=255)
                     gCATCAGCGCCTTCATCGTGCCGACCGATTCGCCGGGCTACCAGGTAGCGCGGGTGGAGGACAAACTCGGCCAGCACGCCTCCGACACCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGt                                                                                                                           <  1:192844/149‑1 (MQ=255)
                      cATCAGCGCCTTCATCGTGCCGACCGATTCGCCGGGCTACCAGGTAGCGCGGGTGGAGGACAAACTCGGCCAGCACGCCTCCGACACCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAgg                                                                                                                             >  5:388463/1‑146 (MQ=255)
                      cATCAGCGCCTTCATCGTGCCGACCGATTCGCCGGGCTACCAGGTAGCGCGGGTGGAGGACAAACTCGGCCAGCACGCCTCCGACACCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGt                                                                                                                           >  7:250467/1‑148 (MQ=255)
                      cATCAGCGCCTTCATCGTGCCGACCGATTCGCCGGGCTACCAGGTAGCGCGGGTGGAGGACAAACTCGGCCAGCACGCCTCCGACACCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGt                                                                                                                           >  3:139608/1‑148 (MQ=255)
                       aTCAGCGCCTTCATCGTGCCGACCGATTCGCCGGGCTACCAGGTAGCGCGGGTGGAGGACAAACTCGGCCAGCACGCCTCCGACACCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTg                                                                                                                          >  7:31120/1‑148 (MQ=255)
                       aTCAGCGCCTTCATCGTGCCGACCGATTCGCCGGGCTACCAGGTAGCGCGGGTGGAGGACAAACTCGGCCAGCACGCCTCCGACACCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGa                                                                                                                         <  1:160775/149‑1 (MQ=255)
                           gcgcCTTCATCGTGCCGACCGATTCGCCGGGCTACCAGGTAGCGCGGGTGGAGGACAAACTCGGCCAGCACGCCTCCGACACCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGc                                                                                                                     >  7:222518/1‑149 (MQ=255)
                              ccTTCATCGTGCCGACCGATTCGCCGGGCTACCAGGTAGCGCGGGTGGAGGACAAACTCGGCCAGCACGCCTCCGACACCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTAc                                                                                                                  <  8:31120/149‑1 (MQ=255)
                                  cATCGTGCCGACCGATTCGCCGGGCTACCAGGTAGCGCGGGTGGAGGACAAACTCGGCCAGCACGCCTCCGACACCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTAc                                                                                                                  >  4:8808/1‑145 (MQ=255)
                                     cGTGCCGACCGATTCGCCGGGCTACCAGGTAGCGCGGGTGGAGGACAAACTCGGCCAGCACGCCTCCGACACCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCg                                                                                                           >  6:89884/1‑149 (MQ=255)
                                          cgaccgaTTCGCCGGGCTACCAGGTAGCGCGGGTGGAGGACAAACTCGGCCAGCACGCCTCCGACACCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTg                                                                                                      >  8:364386/1‑149 (MQ=255)
                                             ccgaTTCGCCGGGCTACCAGGTAGCGCGGGTGGAGGACAAACTCGGCCAGCACGCCTCCGACACCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGcc                                                                                                   <  7:228933/149‑1 (MQ=255)
                                              cgaTTCGCCGGGCTACCAGGTAGCGCGGGTGGAGGACAAACTCGGCCAGCACGCCTCCGACACCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCa                                                                                                  <  5:252164/149‑1 (MQ=255)
                                                   cGCCGGGCTACCAGGTAGCGCGGGTGGAGGACAAACTCGGCCAGCACGCCTCCGACACCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCtt                                                                                             >  7:364830/1‑149 (MQ=255)
                                                    gCCGGGCTACCAGGTAGCGCGGGTGGAGGACAAACTCGGCCAGCACGCCTCCGACACCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCtt                                                                                             >  3:327040/1‑148 (MQ=255)
                                                    gCCGGGCTACCAGGTAGCGCGGGTGGAGGACAAACTCGGCCAGCACGCCTCCGACACCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCtt                                                                                             >  7:234545/1‑148 (MQ=255)
                                                    gCCGGGCTACCAGGTAGCACGGGTGGAGGACAAACTCGGCCAGCACGCCTCCGACACCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCtt                                                                                             <  2:224193/148‑1 (MQ=255)
                                                      cGGGCTACCAGGTAGCGCGGGTGGAGGACAAACTCGGCCAGCACGCCTCCGACACCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCTTGaa                                                                                          <  7:211926/149‑1 (MQ=255)
                                                                     cgcgGGTGGAGGACAAACTCGGCCAGCACGCCTCCGACACCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCTTGAAGGCGGCCGTATCGGc                                                                           <  4:327040/149‑1 (MQ=255)
                                                                       cgGGTGGAGGACAAACTCGGCCAGCACGCCTCCGACACCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCTTGAAGGCGGCCGTATCGGCAt                                                                         <  8:234545/149‑1 (MQ=255)
                                                                        gggTGGAGGACAAACTCGGCCAGCACGCCTCCGACACCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGACCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCTTGAAGGCGGCCGTATCGGCATc                                                                        >  3:152751/1‑149 (MQ=255)
                                                                             gaggaCAAACTCGGCCAGCACGCCTCTGACACCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCTTGAAGGCGGCCGTATCGGCATCGCCTc                                                                   >  5:45930/1‑149 (MQ=255)
                                                                               ggaCAAACTCGGCCAGCACGCCTCCGACACCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCTTGAAGGCGGcc                                                                                   >  8:126791/1‑131 (MQ=255)
                                                                               ggaCAAACTCGGCCAGCACGCCTCCGACACCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCTTGAAGGCGGcc                                                                                   <  7:126791/131‑1 (MQ=255)
                                                                                gaCAAACTCGGCCAGCACGCCTCCGACACCTGCCTGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGa                                                                                                              <  5:288058/103‑1 (MQ=255)
                                                                                gaCAAACTCGGCCAGCACGCCTCCGACACCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGa                                                                                                              >  6:288058/1‑103 (MQ=255)
                                                                                   aaaCTCGGCCAGCACGCCTCCGACACCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAgg                                                                                                                             <  7:186396/85‑1 (MQ=255)
                                                                                   aaaCTCGGCCAGCACGCCTCCGACACCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAgg                                                                                                                             >  8:186396/1‑85 (MQ=255)
                                                                                      cTCGGCCAGCACGCCTCCGACACCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCTTGAAGGCGGCCGTATCGGCATCGCCTCGCAAGCGGt                                                          <  8:364830/149‑1 (MQ=255)
                                                                                       tCGGCCAGCACGCCTCCGACACCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCTTGAAGGCGGCCGTATCGGCATCGCCTCGCAAGCGGTg                                                         >  4:318541/1‑149 (MQ=255)
                                                                                         ggCCAGCACGCCTCCGACACCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCTTGAAGGCGGCCGTATCGGCATCGCCTCGCAAGCGGTg                                                         <  4:139608/147‑1 (MQ=255)
                                                                                                           acCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCTTGAAGGCGGCCGTATCGGCATCGCCTCGCAAGCGGTGGGTATGGCCCGCGCGGCGtt                                     >  8:368899/1‑149 (MQ=255)
                                                                                                           acCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGACCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCTTGAAGGCGGCCGTATCGGCATCGCCTCGCAAGCGGTGGGTATGGCCCGCGCGGCGtt                                     <  4:152751/149‑1 (MQ=255)
                                                                                                             cTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCTTGAAggcg                                                                                      >  6:387347/1‑98 (MQ=255)
                                                                                                                  aGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCTTGAAGGCGGCCGTATCGGCATCGCCTCGCAAGCGGTGGGTATGGCCCGCGCGGCGTTCGAAGTg                              >  7:278661/1‑149 (MQ=255)
                                                                                                                  aGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCTTGAAGGCGGCCGTATCGGCATCGCCTCGCAAGCGGTGGGTATGGCCCGCGCGGCGTTCGAAGTg                              <  7:364386/149‑1 (MQ=255)
                                                                                                                   gATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCTTGAAGGCGGCCGTATCGGCATCGCCTCGCAAGCGGTGGGTATGGCCCGCGCGGCGTTCGAAGTgg                             >  2:146648/1‑149 (MQ=255)
                                                                                                                        ttttCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCTTGAAGGCGGCCGTATCGGCATCGCCTCGCAAGCGGTGGGTATGGCCCGCGCGGCGTTCGAAGTGGCGCgg                        <  3:318541/149‑1 (MQ=255)
                                                                                                                        ttttCGACAATGTGCAAGTGCCAGTGACCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCTTGAAGGCGGCCGTATCGGCATCGCCTCGCAAGCGGTGGGt                                                      <  4:85385/119‑1 (MQ=255)
                                                                                                                        ttttCGACAATGTGCAAGTGCCAGTGACCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCTTGAAGGCGGCCGTATCGGCATCGCCTCGCAAGCGGTGGGt                                                      >  3:85385/1‑119 (MQ=255)
                                                                                                                             gACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCTTGAAGGCGGCCGTATCGGCATCGCCTCGCAAGCGGTGGGTATGGCCCGCGCGGCGTTc                                    >  1:202793/1‑132 (MQ=255)
                                                                                                                             gACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCTTGAAGGCGGCCGTATCGGCATCGCCTCGCAAGCGGTGGGTATGGCCCGCGCGGCGTTc                                    <  2:202793/132‑1 (MQ=255)
                                                                                                                              aCAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCTTGAAGGCGGCCGTATCGGCATCGCCTCGCAAGCGGTGGGTATGGCCCGCGCGGCGTTCGAAGTGGCGCGGGACTAt                  <  1:146648/149‑1 (MQ=255)
                                                                                                                                  tgtgCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCTTGAAGGCGGCCGTATCGGCATCGCCTCGCAAGCGGTGGGTATGGCCCGCGCGGCGTTCGAAGTGGCGCGGGACTATGcc               >  7:64299/1‑148 (MQ=255)
                                                                                                                                  tgtgCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCTTGAAGGCGGCCGTATCGGCATCGCCTCGCAAGCGGTGGGTATGGCCCGCGCGGCGTTCGAAGTGGCGCGGGACTATGcc               >  8:25058/1‑148 (MQ=255)
                                                                                                                                    tgCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCTTGAAGGCGGCCGTATCGGCATCGCCTCGCAAGCGGTGGGTATGGCCCGCGCGGCGTTCGAAGTGGCGCGGGACTATGCCAAc            <  8:278661/149‑1 (MQ=255)
                                                                                                                                            ccAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCTTGAAGGCGGCCGTATCGGCATCGCCTCGCAAGCGGTGGGTATGGCCCGCGCGGCGTTCGAAGTGGCGCGGGACTATGCCAACGAGCGCCa    <  8:222518/149‑1 (MQ=255)
                                                                                                                                             cAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCTTGAAGGCGGCCGTATCGGCATCGCCTCGCAAGCGGTGGGTATGGCCCGCGCGGCGTTCGAAGTGGCGCGGGACAATGCCAACGAgcgc      >  2:339030/1‑146 (MQ=255)
                                                                                                                                              aGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCTTGAAGGCGGCCGTATCGGCATCGCCTCGCAAGCGGTGGGTATg                                                   >  2:383202/1‑100 (MQ=255)
                                                                                                                                              aGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCTTGAAGGCGGCCGTATCGGCATCGCCTCGCAAGCGGTGGGTATg                                                   <  1:383202/100‑1 (MQ=255)
                                                                                                                                              aGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCTTGAAGGCGGCCGTATCGGCATCGCCTCGCAAGCGGTGGGTATGGCCCGCGCGGCGTTCGAAGTGGCGCGGGACTATGCCAACGAGCGCCaga  <  7:368899/149‑1 (MQ=255)
                                                                                                                                                  gCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCTTGAAGGCGGCCGTATCGGCATCGCCTCGCAAGCGGTGGGTATGGCCCGCGCGGCGTTCGAAGTGGCGCGGGACTATGCCAACGAGCGcc     >  7:91946/1‑142 (MQ=255)
                                                                                                                                                  gCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCTTGAAGGCGGCCGTATCGGCATCGCCTCGCAAGCGGTGGGTATGGCCCGCGCGGCGTTCGAAGTGGCGCGGGACTATGCCAACGAGCGcc     <  8:91946/142‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
ACCCGGAGGCCGGCAAGCGTGGCATCAGCGCCTTCATCGTGCCGACCGATTCGCCGGGCTACCAGGTAGCGCGGGTGGAGGACAAACTCGGCCAGCACGCCTCCGACACCTGCCAGATCGTTTTCGACAATGTGCAAGTGCCAGTGGCCAACCGGCTGGGGGCGGAGGGTGAAGGCTACAAGATCGCCCTGGCCAACCTTGAAGGCGGCCGTATCGGCATCGCCTCGCAAGCGGTGGGTATGGCCCGCGCGGCGTTCGAAGTGGCGCGGGACTATGCCAACGAGCGCCAGA  >  NC_002947/2527470‑2527760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑