Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,673,182	A→C	12.3%	M166L (ATG→CTG) ‡	PP_2340 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,673,182	0	A	C	12.3%	254.3 / 27.1	97	M166L (ATG→CTG) ‡	PP_2340	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (43/41);  new base C (5/7);  total (48/49)
Fisher's exact test for biased strand distribution p-value = 7.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.80e-01

CTGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGATCACTTCGCT  >  NC_002947/2673038‑2673329                                                                                                                                                  |                                                                                                                                                    cTGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATggg                                                                                                                                                 <  8:279225/149‑1 (MQ=255)  tGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATggg                                                                                                                                                 >  4:117448/1‑148 (MQ=255)    gAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATggg                                                                                                                                                 <  3:117448/146‑1 (MQ=255)    gAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCa                                                                                                                                               <  4:244399/148‑1 (MQ=255)         cTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGCTGCCCATGGCAtcgcgc                                                                                                                                         <  7:342957/149‑1 (MQ=255)         cTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGCTGCCCATGGCAtcgcgc                                                                                                                                         <  7:146867/149‑1 (MQ=255)         cTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTCCCTGCGCGCTGCCCATGGCAtcgcg                                                                                                                                          >  4:327986/1‑148 (MQ=255)            tGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGCTGCCCATGGCATCGCGCa                                                                                                                                        <  3:386822/147‑1 (MQ=255)            tGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGCTGCCCATGGCATCGCGCAg                                                                                                                                       <  6:50457/148‑1 (MQ=255)            tGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAg                                                                                                                                       <  1:187193/148‑1 (MQ=255)              gCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTCCCTGCGCGCTGCCCATGGCATCGCGCAggaa                                                                                                                                    <  3:327986/149‑3 (MQ=255)                      ttGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑AACGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGc                                                                                                                            <  1:335939/149‑1 (MQ=255)                         ctgcCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCTGCGTTATATCGCCCCCAGCTTCCTGCGCTATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCg                                                                                                                          <  7:259509/148‑1 (MQ=255)                          tgcCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCggg                                                                                                                        >  7:387423/1‑149 (MQ=255)                           gcCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAgc                                                                                                                                             <  6:332014/127‑1 (MQ=255)                           gcCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAgc                                                                                                                                             >  5:332014/1‑127 (MQ=255)                           gcCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGc                                                                                                                       >  1:184536/1‑149 (MQ=255)                            ccGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCg                                                                                                                      <  6:375034/149‑1 (MQ=255)                                    ccGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTc                                                                                                              >  6:42842/1‑149 (MQ=255)                                          cTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGt                                                                                                                                <  6:996/125‑1 (MQ=255)                                          cTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGt                                                                                                                                >  5:996/1‑125 (MQ=255)                                             ggAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGCTGCCCATGGCATCGCGCAg                                                                                                                                       >  8:331667/1‑115 (MQ=255)                                              gAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTAt                                                                                                    >  7:241678/1‑149 (MQ=255)                                              gAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTAt                                                                                                    >  3:131471/1‑149 (MQ=255)                                               aaCTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCATGAGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCCGTCGCCGGTGTAtt                                                                                                   >  8:167035/1‑149 (MQ=255)                                                    ccccAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCaa                                                                                              >  2:19023/1‑149 (MQ=255)                                                    ccccAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCaa                                                                                              <  8:387423/149‑1 (MQ=255)                                                        aaTTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGc                                                                                          >  6:409110/1‑149 (MQ=255)                                                         aactcgcgAT‑CGATCGCATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCt                                                                                         >  1:154276/5‑149 (MQ=255)                                                             cgcgATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTg                                                                                     >  5:171040/1‑149 (MQ=255)                                                              gcgatgcgatCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGc                                                                                                                       <  5:2771/114‑1 (MQ=255)                                                              gcgatgcgatCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGc                                                                                                                       >  6:2771/1‑114 (MQ=255)                                                                gatgcgatCG‑ATCGCGAGTTCTTCCTGCTTTCCGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAg                                                                                  <  3:242327/149‑1 (MQ=255)                                                                  tgcgatCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAAt                                                                                                                   <  3:62726/114‑1 (MQ=255)                                                                  tgcgatCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAAt                                                                                                                   >  4:62726/1‑114 (MQ=255)                                                                     gatcg‑atcGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTc                                                                             >  2:279175/1‑149 (MQ=255)                                                                      atcg‑atcgCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCg                                                                            >  6:340809/1‑149 (MQ=255)                                                                       tcg‑atcgCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGCCAGCGCGCAGCGCCAGTGAGCCGGGCGAATCAGTCGCCCGTGTAt                                                                                                    >  6:210777/1‑124 (MQ=255)                                                                       tcg‑atcgCGAGTGCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTAt                                                                                                    <  5:210777/124‑1 (MQ=255)                                                                            tcgCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCTGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGAt                                                                       <  8:241678/149‑1 (MQ=255)                                                                            tcgCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGAt                                                                       >  6:278358/1‑149 (MQ=255)                                                                            tcgCGAGTTCATCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGAt                                                                       <  4:29490/149‑1 (MQ=255)                                                                                gAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCg                                                                    >  6:112593/1‑148 (MQ=255)                                                                                    ccttcCTGCTTTCAGTGTCGCACTACCAGCGCTATATCGCCCCCAGGTTCCTGCGCGATGCCATGGGCAGCGCGGAGGGCCAGTGAGGCGGGCGAATCATTCGCCGGTGTATTCGCAACCGTTGGTGCAGGTTTCGTGGATTCGAACCt                                                               <  1:219745/148‑1 (MQ=255)                                                                                     cttcCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCt                                                               <  8:325667/148‑1 (MQ=255)                                                                                      ttcCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTg                                                             >  3:292433/1‑149 (MQ=255)                                                                                      ttcCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTg                                                             >  1:350120/1‑149 (MQ=255)                                                                                      ttcCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCCGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTg                                                             >  6:367394/1‑149 (MQ=255)                                                                                      ttcCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTCCCTGCGCGCTGCCCATGGCATCGCGCAggaag                                                                                                                                   <  5:422798/79‑4 (MQ=21)                                                                                      ttcCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTCCCTGCGCGCTGCCCATGGCATCGCGCAggaag                                                                                                                                   >  6:422798/1‑76 (MQ=21)                                                                                        cttGCTTTCAGCGGCGCACTACCAGCGCTATATCGCCCCCCGCTTCCTGCGCTATGCCTTGGGCAGGGCGCAGGGCCAGTGAGCCTGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCtt                                                              <  2:184536/144‑1 (MQ=255)                                                                                        ccTGGTTTCAGAGTCGCACTACCAGCGGTATATCGCCCCGAGCTTCCTGCGCTTAGCCATGGGCAGCGCGAAGGGCCAGTGAGCGGTGCGAATCAGTCGCCGGTGTATTCGCAACCGGTGGTGCAGGTTTCGt                                                                           <  7:232913/133‑1 (MQ=255)                                                                                        ccTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCtt                                                              <  4:131471/146‑1 (MQ=255)                                                                                        ccTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTg                                                             <  4:292433/147‑1 (MQ=255)                                                                                        ccTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTg                                                             <  1:19023/147‑1 (MQ=255)                                                                                        ccTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTACTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGt                                                                           >  8:232913/1‑133 (MQ=255)                                                                                         cTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGAc                                                          <  4:380811/149‑1 (MQ=255)                                                                                          tGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGa                                                           >  3:349276/1‑147 (MQ=255)                                                                                            cTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGc                                                       >  4:127750/1‑149 (MQ=255)                                                                                                  gagGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGc                                                 <  6:171040/149‑1 (MQ=255)                                                                                                  gagGCGCACTACCAGCGCTATATCG‑CCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCa                                                >  7:387922/1‑149 (MQ=255)                                                                                                   agGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGc                                                 <  2:350120/148‑1 (MQ=255)                                                                                                   agGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCa                                                >  6:2643/1‑149 (MQ=255)                                                                                                    ggCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCAt                                               >  7:195906/1‑149 (MQ=255)                                                                                                     gcgcACTACCAGCGATATATCCCCCCCCGCGTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATc                                              <  2:349671/149‑1 (MQ=255)                                                                                                      cgcACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCa                                             <  8:195906/149‑1 (MQ=255)                                                                                                       gcACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAg                                            >  1:222671/1‑149 (MQ=255)                                                                                                             ccAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGc                                        <  1:279175/147‑1 (MQ=255)                                                                                                             ccAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCtt                                      <  3:127750/149‑1 (MQ=255)                                                                                                               aGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGa                                    >  4:17232/1‑149 (MQ=255)                                                                                                                gcgcTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACa                                                         >  5:240185/1‑127 (MQ=255)                                                                                                                gcgcTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGAc                                   <  5:340809/149‑1 (MQ=255)                                                                                                                    tataTCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTgg                               >  2:175996/1‑149 (MQ=255)                                                                                                                      tatCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCtt                                      <  3:32912/140‑1 (MQ=255)                                                                                                                      tatCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCtt                                      >  4:32912/1‑140 (MQ=255)                                                                                                                        tCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTcc                            >  3:212559/1‑148 (MQ=255)                                                                                                                          gCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTcc                            >  2:141569/1‑146 (MQ=255)                                                                                                                           cccccAGCTTCCTGCGCGTTGCCATGGGAAGCGCGCAGGGCCAGTGAGCCCGGCGAATCAGTCCCCGGTGTATTAGCCACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTGCGGCATCAGCGGCTTGACCTGGTcc                            >  5:84316/1‑145 (MQ=255)                                                                                                                           cccccAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGa                        <  5:278358/149‑1 (MQ=255)                                                                                                                           cccccAGCTTCCCGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGa                        <  5:367394/149‑1 (MQ=255)                                                                                                                            ccccAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGAt                       <  1:175996/149‑1 (MQ=255)                                                                                                                              ccAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTg                                                                                        <  2:142972/82‑1 (MQ=255)                                                                                                                              ccAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTg                                                                                        >  1:142972/1‑82 (MQ=255)                                                                                                                                aGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCAc                   <  5:409110/149‑1 (MQ=255)                                                                                                                                    cccTGCGCGCTGCCCATGGCATCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTgg               >  2:61501/2‑149 (MQ=255)                                                                                                                                      cTGCGCGCTGCCCATGGCATCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTc                                                                                                  >  2:232139/1‑64 (MQ=21)                                                                                                                                      cTGCGCGCTGCCCATGGCATCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTc                                                                                                  <  1:232139/64‑1 (MQ=21)                                                                                                                                      cTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGc              <  4:212559/148‑1 (MQ=255)                                                                                                                                       tGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGa            >  8:46422/1‑149 (MQ=255)                                                                                                                                         cgcgATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGAt           <  5:42842/148‑1 (MQ=255)                                                                                                                                           cgATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGATCAc        <  7:46422/149‑1 (MQ=255)                                                                                                                                            gATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGATc          >  5:251632/1‑146 (MQ=255)                                                                                                                                            gATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGATc          <  6:251632/146‑1 (MQ=255)                                                                                                                                                ccATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGATCACTTCGc   >  3:367669/1‑149 (MQ=255)                                                                                                                                                ccATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGATCACTTCGc   >  5:285927/1‑149 (MQ=255)                                                                                                                                                 cATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGATCACTTCGCt  <  5:270363/149‑1 (MQ=255)                                                                                                                                                 cATGGGCAGCGAGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGAAAGCTCCGGCATCATCGGCTTTACCTGGTCCCAGCTACACTTGGCGATCAATTctct  >  6:326193/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                    CTGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGATCACTTCGCT  >  NC_002947/2673038‑2673329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑