Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,700,158 G→A 8.1% A118V (GCT→GTT)  fsr‑II ← fosmidomycin efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,700,1580GA 8.1% 139.8 / 2.3 49A118V (GCT→GTT) fsr‑IIfosmidomycin efflux system
Reads supporting (aligned to +/- strand):  ref base G (25/20);  new base A (2/2);  total (27/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.42e-01

CCGCCGACCTGGAAGGTGGATTGCGCGGTACCGAAGCGCCCGCCGGAAGCCATTCTGGCCACCCGTGAGGCCTCTGGGTGAAAGGTGGCCGAACCCACGCCGACCACGGCGGCAGCCACCAGCAGCATTTCATAGTTGCCAGCAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTAGGGTTGTGGGTGCTTGTCGGTGAACATGCCGACCCAAGGTTGCAGCAGGGAAGCGGTCACCTGATACACCAAGGCGATCCAGCCGATCTG  >  NC_002947/2700017‑2700306
                                                                                                                                             |                                                                                                                                                    
ccgccgACCTGGAAGGTGGATTGCGCGGTACCGAAGCGCCCGCCGGAAGCCATTCTGGCCACCCGTGAGGCCTCTGGGTGAAAGGTGGCCGAACCCACGCCGACCACGGCGGCAGCCACCAGCAGCATTTCATAGTTGCCAGCAAAGGc                                                                                                                                               <  6:316784/149‑1 (MQ=255)
ccgccgACCTGGAAGGTGGATTGCGCGGTACCGAAGCGCCCGCCGGAAGCCATTCTGGCCACCCGTGAGGCCTCTGGGTGAAAGGTGGCCGAACCCACGCCGACCACGGCGGCAGCCACCAGCAGCATTTCATAGTTGCCAGCAAAGGc                                                                                                                                               <  7:23830/149‑1 (MQ=255)
  gccgACCTGGAAGGTGGATTGCGCGGTACCGAAGCGCCCGCCGGAAGCCATTCTGGCCACCCGTGAGGCCTCTGGGTGAAAGGTGGCCGAACCCACGCCGACCACGGCGGCAGCCACCAGCAGCATTTCATAGTTGCCAGCAAAGGc                                                                                                                                               >  4:272975/1‑147 (MQ=255)
           gAAGGTGGATTGCGCGGTACCGAAGCGCCCGCCGGAAGCCATTCTGGCCACCCGTGAGGCCTCTGGGTGAAAGGTGGCCGAACCCACGCCGACCACGGCGGCAGCCACCAGCAGCATTTCATAGTTGCCAGCAAAGGCGAGCAGGGCg                                                                                                                                     <  1:58620/148‑1 (MQ=255)
           gAAGGTGGATTGCGCGGTACCGAAGCGCCCGCCGGAAGCCATTCTGGCCACCCGTGAGGCCTCTGGGTGAAAGGTGGCCGAACCCACGCCGACCACGGCGGCAGCCACCAGCAGCATTTCATAGTTGCCAGCAAAGGCGAGCAGGGCg                                                                                                                                     >  2:58620/1‑148 (MQ=255)
              ggTGGATTGCGCGGTACCGAAGCGCCCGCCGGAAGCCATTCTGGCCACCCGTGAGGCCTCTGGGTGAAAGGTGGCCGAACCCACGCCGACCACGGCGGCAGCCACCAGCAGCATTTCATAGTTGCCAGCAAAGGCGAGCAggg                                                                                                                                       <  1:143489/143‑1 (MQ=255)
              ggTGGATTGCGCGGTACCGAAGCGCCCGCCGGAAGCCATTCTGGCCACCCGTGAGGCCTCTGGGTGAAAGGTGGCCGAACCCACGCCGACCACGGCGGCAGCCACCAGCAGCATTTCATAGTTGCCAGCAAAGGCGAGCAggg                                                                                                                                       >  2:143489/1‑143 (MQ=255)
                  gATTGCGCGGTACCGAAGCGCCCGCCGGAAGCCATTCTGGCCACCCGTGAGGCCTCTGGGTGAAAGGTGGCCGAACCCACGCCGACCACGGCGGCAGCCACCAGCAGCATTTCATAGTTGCCAGCAAAGGCGAGCAGGGCGATACccac                                                                                                                             >  4:64057/1‑149 (MQ=255)
                                 aaGCGCCCGCCGGAAGCCATTCTGGCCACCCGTGAGGCCTCTGGGTGAAAGGTGGCCGAACCCACGCCGACCACGGCGGCAGCCACCAGCAGCATTTCATAGTTGCCAGCAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCAt                                                                                                              <  3:280799/149‑1 (MQ=255)
                                 aaGCGCCCGCCGGAAGCCATTCTGGCCACCCGTGAGGCCTCTGGGTGAAAGGTGGCCGAACCCACGCCGACCACGGCGGCAGCCACCAGCAGCATTTCATAGTTGCCAGCAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCAt                                                                                                              <  3:275415/149‑1 (MQ=255)
                                             gAAGCCATTCTGGCCACCCGTGAGGCCTCTGGGTGAAAGGTGGCCGAACCCACGCCGACCACGGCGGCAGCCACCAGCAGCATTTCATAGTTGCCAGCAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTaa                                                                                                  >  5:232920/1‑149 (MQ=255)
                                                         gCCACCCGTGAGGCCTCTGGGTGAAAGGTGGCCGAACCCACGCCGACCACGGCGGCAGCCACCAGCAGCATTTCATAGTTGCCAGCAAAGGCGAGCa                                                                                                                                          >  4:82678/1‑97 (MQ=255)
                                                         gCCACCCGTGAGGCCTCTGGGTGAAAGGTGGCCGAACCCACGCCGACCACGGCGGCAGCCACCAGCAGCATTTCATAGTTGCCAGCAAAGGCGAGCa                                                                                                                                          <  3:82678/97‑1 (MQ=255)
                                                            aCCCGTGAGGCCTCTGGGTGAAAGGTGGCCGAACCCACGCCGACCACGGCGGCAGCCACCAGCAGCATTTCATAGTTGCCAGCAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTAGGGTTGTgg                                                                                   <  3:272975/149‑1 (MQ=255)
                                                                   aGGCCTCTGGGTGAAAGGTGGCCGAACCCACGCCGACCACGGCGGCAGCCACCAGCAGCATTTCATAGTTGCCAGCAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTAGGGTTGTGGGTGCtt                                                                             <  3:64057/148‑1 (MQ=255)
                                                                       ctctGTGTGAAAGGTGTCCGAACCCACGCCGAACACGGCGGCAGCCACCAGCAGCATTTCATAGTTGCCAGCAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTAGGGTTGTGGGTGCTTGTCg                                                                         <  2:189348/148‑1 (MQ=255)
                                                                       ctctGGGTGAAAGGTGGCCGAACCCACGCCGACCACGGCGGCAGCCACCAGCAGCATTTCATAGTTGCGAACAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTAGGGTTGTGGGTGCtt                                                                             <  6:394308/144‑1 (MQ=255)
                                                                       ctctGGGTGAAAGGTGGCCGAACCCACGCCGACCACGGCGGCAGCCACCAGCAGCATTTCATAGTTGCCAGCAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGt                                                                                             >  6:415147/1‑128 (MQ=255)
                                                                       ctctGGGTGAAAGGTGGCCGAACCCACGCCGACCACGGCGGCAGCCACCAGCAGCATTTCATAGTTGCCAGCAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGt                                                                                             <  5:415147/128‑1 (MQ=255)
                                                                       ctctGGGTGAAAGGTGGCCGAACCCACGCCGACCACGGCGGCAGCCACCAGCAGCATTTCATAGTTGCCAACAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTAGGGTTGTGGGTGCtt                                                                             <  6:400261/144‑1 (MQ=255)
                                                                       ctctGGGTGAAAGGTGGCCGAACCCACGCCGACCACGGCGGCAGCCACCAGCAGCATTTCATAGTTGCCAACAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTAGGGTTGTGGGTGCtt                                                                             >  5:394308/1‑144 (MQ=255)
                                                                       ctctGGGTGAAAGGTGGCCGAACCCACGCCGACCACGGCGGCAGCCACCAGCAGCATTTCATAGTTGCAAACAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTAGGGTTGTGGGTGCtt                                                                             >  5:400261/1‑144 (MQ=255)
                                                                        tctGGGTGAAAGGTGGCCGAACCCACGCCGACCACGGCGGCAGCCACCAGCAGCATTTAATAGTTGCCAGCAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTAGGGTTGTGGGTGCTTGACgt                                                                        >  1:189348/1‑147 (MQ=255)
                                                                          tGGGTGAAAGGTGGCCGAACCCACGCCGACCACGGCGGCAGCCACCAGCAGCATTTCATAGTTGCCAGCAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTAGGGTTGTGGGTGCTTGTCGGt                                                                       <  6:232920/147‑1 (MQ=255)
                                                                                         cGAACCCACGCCGACCACGGCGGCAGCCACCAGCAGCATTTCATAGTTGCCAGCAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTAGGGTTGTGGGTGCTTGTCGGTGAACATGCCGACCCAAg                                                      >  8:200109/1‑149 (MQ=255)
                                                                                         cGAACCCACGCCGACCACGGCGGCAGCCACCAGCAGCATTTCATAGTTGCCAGCAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTAGGGTTGTGGGTGCTTGTCGGTGAACATGCCGACCCAAg                                                      >  8:300457/1‑149 (MQ=255)
                                                                                           aaCCCACGCCGACCACGGCGGCAGCCACCAGCAGCATTTCATAGTTGCCAGCAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTAGGGTTGTGGGt                                                                                 >  1:378442/1‑120 (MQ=255)
                                                                                           aaCCCACGCCGACCACGGCGGCAGCCACCAGCAGCATTTCATAGTTGCCAGCAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTAGGGTTGTGGGt                                                                                 <  2:378442/120‑1 (MQ=255)
                                                                                              ccACGCAGACCACGGCGGCAGCCACCAGCAGCATTTCATAGTTGCGAGCAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTATGGTTGTgg                                                                                   <  3:335181/115‑1 (MQ=255)
                                                                                                aCGCCGACCACGGCGGCAGCCACCAGCAGCATTTCATAGTTGCCAGCAAAGGCGAGCAGGGAGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTAGGGTTGTGGGTGCTTGTCGGTGAACATGCCGACCCAAGGTTgcag                                               >  8:54474/1‑149 (MQ=255)
                                                                                                  gCCGACCACGGCGGCAGCCACCAGCAGCATTTCATAGTTGCCAGCAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTAGGGTTGTGGGTGCTTGTCGGTGAACATGCCGACCCAAGGTTgcag                                               >  3:103746/1‑147 (MQ=255)
                                                                                                    cGACCACGGCGGCAGCCACCAGCAGCATTTCATAGTTGCCAGCAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTAGGGTTGTGGGTGCTTGTCGGTGAACATGCCGACCCAAGGTTGCAGCAgg                                           >  4:332154/1‑149 (MQ=255)
                                                                                                     gACCACGGCGGCAGCCACCAGCAGCATTTCATAGTTGCCAGCAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTAGGGTTGTGGGTGCTTGTCGGTGAACATGCCGACCCAAGGTTGCAGCAgg                                           >  4:108153/1‑148 (MQ=255)
                                                                                                       ccACGGCGGCAGCCACCAGCAGCATTTCATAGTTGCCAGCAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTAGGGTTGTGGGTGCTTGTCGGTGAACATGCCGACCCAAGGTTGCAGCAGGGaa                                        <  3:20673/149‑1 (MQ=255)
                                                                                                        cACGGCGGCAGCCACCAGCAGCATTTCATAGTTGCCAGCAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTAGGGTTGTGGGTGCTTGTCGGTGAACATGCCGACCCAAGGTTGCAGCAGGGAAg                                       >  4:171073/1‑149 (MQ=255)
                                                                                                         aCGGCGGCAGCCACCAGCAGCATTTCATAGTTGCCAGCAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTAGGGTTGTGGGTGCTTGTCGGTGAACATGCCGACCCAAGGTTGCAGCAGGGAAGc                                      >  7:383731/1‑149 (MQ=255)
                                                                                                               gcAGCCACCAGCAGCATTTCATAGTTGCCAGCAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTAGGGTTGTGGGTGCTTGTCGGTGAACATGCCGACCCAAGGTTGCAGCAGGGAAGCGGTCAc                                <  7:200109/149‑1 (MQ=255)
                                                                                                                   ccaccaGCAGCATTTCATAGTTGCCAGCAAACGAGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTAGGGTTGTCGGTGCTTCTCGGTGAACATGCCGACCCAAGGTTGCAGCAGGGAAGCGGTCACCTGa                            >  8:155595/1‑149 (MQ=255)
                                                                                                                    caccaGCAGCATTTCATAGTTGCCAGCAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTAGGGTTGTGGGTGCTTGTCGGTGAACATGCCGACCCAAGGTTGCAGCAGGGAAGCGGTCACCTGAt                           <  3:171073/149‑1 (MQ=255)
                                                                                                                    caccaGCAGCATTTCATAGTTGCCAGCAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTAGGGTTGTGGGTGCTTGTCGGTGAACATGCCGACCCAAGGTTGCAGCAGGGAAGCGGTCACCTGAt                           <  2:63741/149‑1 (MQ=255)
                                                                                                                       cagcagCATTTCATAGTTGCCAGCAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTAGGGTTGTGGGTGCTTGTCGGTGAACATGCCGACCCAAGGTTGCAGCAGGGAAGCGGTCACCTGATaca                        >  4:148415/1‑149 (MQ=255)
                                                                                                                         gcagcaTTTCATAGTTGCCAGCAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTAGGGTTGTGGGTCCTTGTCGGTGAACATGCCGACACAAGGTTGCAGCAGGGAAGCGGTCACCTGATacac                       >  1:270730/1‑148 (MQ=255)
                                                                                                                             caTTTCATAGTTGCCAGCAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTAGGGTTGTGGGTGCTTGTCGGTGAACATGCCGACCCAAGGTTGCAGCAGGGAAGCGGTCACCTGATACACCAAgg                  <  3:148415/149‑1 (MQ=255)
                                                                                                                             caTTTCATAGTTGCCAGCAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTAGGGTTGTGGGTGCTTGTCGGTGAACATGCCGACCCAAGGTTGCAGCAGGGAAGCGGTCACCTGATACACCAAgg                  >  7:2802/1‑149 (MQ=255)
                                                                                                                               tttCATAGTTGCCAGCAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTAGGGTTGTGGGTGCTTGTCGGTGAACATGCCGACCCAAGGTTGCAGCAGGGAAGCGGTCACCTGATACACCAAGGc                 >  3:224100/1‑148 (MQ=255)
                                                                                                                                ttCATAGTTGCCAGCAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTAGGGTTGTGGGTGCTTGTCGGTGAACATGCCGACCCAAGGTTGACGCAGGGAAGCGGTAACCTGATACACCAAGGCGa               >  4:234291/1‑149 (MQ=255)
                                                                                                                                     aGTTGCCAGCAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTAGGGTTGTGGGTGCTTGTCGGTGAACATGCCGACCCAAGGTTGcagcag                                            <  4:363317/115‑1 (MQ=255)
                                                                                                                                     aGTTGCCAGCAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTAGGGTTGTGGGTGCTTGTCGGTGAACATGCCGACCCAAGGTTGcagcag                                            >  3:363317/1‑115 (MQ=255)
                                                                                                                                      gTTGCCAGCAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTAGGGTTGTGGGTGCTTGTCGGTGAACATGCCGACCCAAGGTTGCAGCAGGGAAGCGGTCACATGATACACCAAGGCGATCCAGc         >  6:221594/1‑149 (MQ=255)
                                                                                                                                             gCAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTAGGGTTGTGGGTGCTTGTCGGTGAACATGCCGACCCAAGGTTGCAGCAGGGAAGCGGTCACCTGATACACCAAGGCGATCCAGCCGATCTg  >  2:97496/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
CCGCCGACCTGGAAGGTGGATTGCGCGGTACCGAAGCGCCCGCCGGAAGCCATTCTGGCCACCCGTGAGGCCTCTGGGTGAAAGGTGGCCGAACCCACGCCGACCACGGCGGCAGCCACCAGCAGCATTTCATAGTTGCCAGCAAAGGCGAGCAGGGCGATACCCACCAGCGTCACCAGCATGCCGGCAGGTAACAGGTAGGGTTGTGGGTGCTTGTCGGTGAACATGCCGACCCAAGGTTGCAGCAGGGAAGCGGTCACCTGATACACCAAGGCGATCCAGCCGATCTG  >  NC_002947/2700017‑2700306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑