Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,732,333 C→T 6.1% A557V (GCC→GTC)  polB → DNA polymerase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,732,3330CT 6.1% 192.8 / 2.2 65A557V (GCC→GTC) polBDNA polymerase II
Reads supporting (aligned to +/- strand):  ref base C (28/33);  new base T (2/2);  total (30/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.11e-01

GGTTGCCGTTTCTTCGACCCGCGCCTGGCGTCGTCGATCACCATGCGCGGCCACCAGATCATGCGCCAGACCAGGTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAATGGTGGCAAGCCCATCTGCACGAGACCATGAACCTGCACAGCGCCCTCGAACTGCAGTTCGAGGTGCACTACCGGCGCTTCCTGATG  >  NC_002947/2732185‑2732478
                                                                                                                                                    |                                                                                                                                                 
ggTTGCCGTTTCTTCGACCCGCGCCTGGCGTCGTCGATCACCATGCGCGGCCACCAGATCATGCGCCAGACCAGGTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGc                                                                                                                                                   <  1:363154/149‑1 (MQ=255)
    gCCGTTTCTTCGACCCGCGCCTGGCGTCGTCGATCACCATGCGCGGCCACCAGATCATGCGCCAGACCAGGTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCAc                                                                                                                                               >  8:290078/1‑149 (MQ=255)
     ccGTTTCTTCGACCCGCGCCTGGCGTCGTCGATCACCATGCGCGGCCACCAGATCATGCGCCAGACCAGGTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGTCCACg                                                                                                                                              >  3:91538/1‑149 (MQ=255)
     ccGTATCATCGACCCGCGCCTGGCGTCGTCGATCACCATGCGCGGCCACCAGATCATGCGCCAGACCAGGTCCCAGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCGCCCTTTGTCAGGCTCAAGCGTGCCCACg                                                                                                                                              >  7:357919/1‑149 (MQ=255)
          tcttcGACCCGCGCCTGGCGTCGTCGATCACCATGCGCGGCCACCAGATCATGCGCCAGACCAGGTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGa                                                                                                                                          <  4:146436/148‑1 (MQ=255)
            ttcGACCCGCGCCTGGCGTCGTCGATCACCATGCGCGGCCACCAGATCATGCGCCAGACCAGGTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAgg                                                                                                                                        >  2:326780/1‑148 (MQ=255)
            ttcGACCCGCGCCTGGCGTCGTCGATCACCATGCGCGGCCACCAGATCATGCGCCAGACCAGGTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAgg                                                                                                                                        >  3:110424/1‑148 (MQ=255)
            ttcGACCCGCGCCTGGCGTCGTCGATCACCATGCGCGGCCACCAGATCATGCGCCAGACCAGGTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCAACGCCGAgg                                                                                                                                        >  5:403829/1‑148 (MQ=255)
                 cccGCGCCTGGCGTCGTCGATCACCATGCGCGGCCACCAGATCATGCGCCAGACCAGGTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGAcg                                                                                                                                  >  2:13040/1‑149 (MQ=255)
                  ccGCGCCTGGCGTCGTCGATCACCATGCGCGGCCACCAGATCATGCGCCAGACCAGGTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGAcgc                                                                                                                                 >  4:179547/1‑149 (MQ=255)
                  ccGCGCCTGGCGTCGTCGATCACCATGCGCGGCCACCAGATCATGCGCCAGACCAGGTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGAc                                                                                                                                   <  8:239865/147‑1 (MQ=255)
                  ccGCGCCTGGCGTCGTCGATCACCATGCGCGGCCACCAGATCATGCGCCAGACCAGGTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGAc                                                                                                                                   >  7:239865/1‑147 (MQ=255)
                    gcgcCTGGCGTCGTCGATCACCATGCGCGGCCACCAGATCATGCGCCAGACCAGGTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACgcgg                                                                                                                               >  2:213947/1‑149 (MQ=255)
                                gtcgATCACCATGCGCGGCCACCAGATCATGCGCCAGACCAGGTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGc                                                                                                                    <  3:112459/148‑1 (MQ=255)
                                      cACCATGCGCGGCCACCAGATCATGCGCCAGACCAGGTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAAc                                                                                                             >  7:41347/1‑149 (MQ=255)
                                       aCCATGCGCGGCCACCAGATCATGCGCCAGACCAGGTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGTCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAAc                                                                                                             <  4:91538/148‑1 (MQ=255)
                                        ccATGCGCGGCCACCAGATCATGCGCCAGACCAGGTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCgag                                                                                                                <  7:136086/144‑1 (MQ=255)
                                        ccATGCGCGGCCACCAGATCATGCGCCAGACCAGGTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCgag                                                                                                                >  8:136086/1‑144 (MQ=255)
                                           tGCGCGGCCACCAGATCATGCGCCAGACCAGGTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACtggtg                                                                                                        <  6:194363/149‑1 (MQ=255)
                                             cgctGCCACCAGATCATGCGCCAGACCAGGTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACtggtgg                                                                                                       <  8:41347/148‑1 (MQ=255)
                                                  ccaccaGATCATGCGCCAGACCAGGTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGTCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAgg                                                                                                 >  2:138085/1‑149 (MQ=255)
                                                     ccaGATCATGCGCCAGACCAGGTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGTCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCa                                                                                              <  7:132688/149‑1 (MQ=255)
                                                     ccaGATCATGCGCCAGACCAGGTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCa                                                                                              <  8:216806/149‑1 (MQ=255)
                                                      caGATCATGCGCCAGACCAGGTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCga                                                                                                                 <  3:48905/129‑1 (MQ=255)
                                                      caGATCATGCGCCAGACCAGGTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCga                                                                                                                 >  4:48905/1‑129 (MQ=255)
                                                            aTGCGCCAGACCAGGTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCTGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAAtg                                                                                       <  3:34407/149‑1 (MQ=255)
                                                            aTGCGCCAGACCAGGTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAAtg                                                                                       <  2:16930/149‑1 (MQ=255)
                                                            aTGCGCCAGACCAGGTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAAt                                                                                        <  3:51767/148‑1 (MQ=255)
                                                            aTGCGCCAGACCAGGTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAAt                                                                                        <  1:326780/148‑1 (MQ=255)
                                                               cggcAGACCAGGTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAAtggt                                                                                     <  7:388508/145‑1 (MQ=255)
                                                               cgcCAGACCAGGTCCCTGATCGAGGCGTGTGGCTACGATGTCATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAAtggtg                                                                                    <  5:355385/149‑1 (MQ=255)
                                                                 ccAGACCAGGTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAATGGTGGc                                                                                  <  2:182731/149‑1 (MQ=255)
                                                                     aCCAGGTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAAtggt                                                                                     >  7:85305/1‑142 (MQ=255)
                                                                     aCCAGGTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAAtggt                                                                                     <  8:85305/142‑1 (MQ=255)
                                                                       cAGGTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAATGGTGGCAAGccc                                                                            <  1:13040/149‑1 (MQ=255)
                                                                         ggTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGccc                                                                                                                                                 >  2:375571/1‑78 (MQ=255)
                                                                         ggTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGccc                                                                                                                                                 <  1:375571/78‑1 (MQ=255)
                                                                            cccTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCAAGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCATCCAATGGTGGCAAGCCGATCTg                                                                       >  5:234232/1‑149 (MQ=255)
                                                                             ccTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACtg                                                                                                           >  6:196740/1‑112 (MQ=255)
                                                                             ccTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACtg                                                                                                           <  5:196740/112‑1 (MQ=255)
                                                                             ccTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAATGGTGGCAAGCCCATCTg                                                                       >  5:363134/1‑148 (MQ=255)
                                                                             ccTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAATGGTGGCAAGCCCATCTg                                                                       >  3:17246/1‑148 (MQ=255)
                                                                                        cGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAATGGTGGCAAGCCCATCTGCACGAGACCATg                                                           <  4:110424/149‑1 (MQ=255)
                                                                                          tgtgGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAAt                                                                                        >  3:395187/1‑118 (MQ=255)
                                                                                          tgtgGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAAt                                                                                        <  4:395187/118‑1 (MQ=255)
                                                                                              gCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAATGGTGGCAAGCCCATCTGCACGAGACCATGAACCTg                                                     <  7:290078/149‑1 (MQ=255)
                                                                                                tACGATGTGATCTACTGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAATGGTGGCAAGCCCATCTGCACGAGACCATGAACCTGca                                                   >  6:17318/1‑149 (MQ=255)
                                                                                                            tACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAATGGTGGCAAGCCCATCTGCACGAGACCATGAACCTGCACAGCGCCCTCg                                        >  2:58658/1‑148 (MQ=255)
                                                                                                              cGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGTCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAATGGTGGCAAGCCCATCTGCACGAGACCATGAACCTGCACAGCGCCCTc                                         <  1:138085/145‑1 (MQ=255)
                                                                                                              cGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAATGGTGGCaa                                                                                >  5:149265/1‑106 (MQ=255)
                                                                                                              cGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAATGGTGGCaa                                                                                <  6:149265/106‑1 (MQ=255)
                                                                                                              aggTGCCACCGACTCCACCTTTTTCTGGCTGAAGGGTGCACAGGCCGTGGAAGTCGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAATGGTGGCAAGCCCATCTGCACGAGACCATGAACCTGCACAGCGCCCTCGaa                                      <  8:357919/147‑1 (MQ=255)
                                                                                                                 tGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAATGGTGGCAAGCCCATCTGCACGAGACCATGAACCTGCACAGCGCCCTCg                                        <  7:249261/143‑1 (MQ=255)
                                                                                                                 tGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAATGGTGGCAAGCCCATCTGCACGAGACCATGAACCTGCACAGCGCCCTCg                                        >  8:249261/1‑143 (MQ=255)
                                                                                                                   acacCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAAtggt                                                                                     <  1:145796/96‑1 (MQ=255)
                                                                                                                   acacCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAAtggt                                                                                     >  2:145796/1‑96 (MQ=255)
                                                                                                                       cGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAATGGTGGCAAGCCCATCTGCACGAGACCATGAACCCGCACAGCGCCCTCGAACTGCa                                 >  8:73843/1‑144 (MQ=255)
                                                                                                                       cGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAATGGTGGCAAGCCCATCTGCACGAGACCATGAACCCGCACAGCGCCCTCGAACTGCa                                 <  7:73843/144‑1 (MQ=255)
                                                                                                                          cTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAATGGTGGCAAGCCCATCTGCACGAGACCATGAACCTGCACAGCGCCCTCGAACTGCAGTTCGAgg                         <  6:403829/149‑1 (MQ=255)
                                                                                                                             cACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAATGGTGGCAAGCCCATCTGCACGAGACCATGAACCTGCACAGCGCCCTCGAACTGCAGTTCGAggg                        <  1:58658/147‑2 (MQ=255)
                                                                                                                              aCCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAAc                                                                                            <  5:143136/78‑1 (MQ=255)
                                                                                                                              aCCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAAc                                                                                            >  6:143136/1‑78 (MQ=255)
                                                                                                                                       tGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAATGGTGGCAAGCCCATCTGCACGAGACCATGAACCTGca                                                   <  6:300206/110‑1 (MQ=255)
                                                                                                                                       tGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAATGGTGGCAAGCCCATCTGCACGAGACCATGAACCTGca                                                   >  5:300206/1‑110 (MQ=255)
                                                                                                                                           tCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAATGGTGGCAAGCCCATCTGCACGAGACCATGAACCTGCACAGCGCCCTCGAACTGCAGTTCGAGGTGCACTACCGGCGCTTc        >  5:240461/1‑149 (MQ=255)
                                                                                                                                           tCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAATGGTGGCAAGCCCATCTGCACGAGACCATGAACCTGCACAGCGCCCTCGAACTGCAGTTCGAGGTGCACTACCGGCGCTTc        >  4:220897/1‑149 (MQ=255)
                                                                                                                                                ggTTCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAATGGTGGCAAGCCCATCTGCACGAGACCATGAACCTGCACAGCGCCCTCGAACTGCAGTTCGAGGTGCACTACCGGCGCTTCCTGAt   <  8:207276/149‑1 (MQ=255)
                                                                                                                                                 gTGCCCACGCAGAGGAAGACGCGGCTCTCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAATGGTGGCAAGCCCATCTGCACGAGACCATGAACCTGCACAGCGCCCTCGAACTGCAGTTCGAGGTGCACTACCGGCGCTTCCTGATg  <  6:240461/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GGTTGCCGTTTCTTCGACCCGCGCCTGGCGTCGTCGATCACCATGCGCGGCCACCAGATCATGCGCCAGACCAGGTCCCTGATCGAGGCGTGTGGCTACGATGTGATCTACGGTGACACCGACTCCACCTTTGTCTGGCTCAAGGGTGCCCACGCCGAGGAAGACGCGGCGCGCATTGGCCGAGAACTGGTGGCAAAGGTCAACCAATGGTGGCAAGCCCATCTGCACGAGACCATGAACCTGCACAGCGCCCTCGAACTGCAGTTCGAGGTGCACTACCGGCGCTTCCTGATG  >  NC_002947/2732185‑2732478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑