| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,741,751 | T→G | 6.3% | L1377W (TTG→TGG) | PP_2395 → | leucine‑rich repeat‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,741,751 | 0 | T | G | 6.3% | 186.7 / 2.6 | 61 | L1377W (TTG→TGG) | PP_2395 | leucine‑rich repeat‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (28/28); new base G (2/2); total (31/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
GCTAGACCTGGGCAGGCGTTTATGGCGCCAGGATGCAGTAGACCTGTTCGCTACCCGATGGGCGCTACAGCATGGCAGGGCGCTGGAAGGAAGCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGACCTTGCACAAGCAGAGGCTGC‑‑GGTGCGGGATGCAGAAACCCCGGAAGAGGTTGCCCGCTCCATGGTTGACCGTGAATTCTGGCAGGCGCATC > NC_002947/2741605‑2741894 | gCTAGACCTGGGCAGGCGTTTATGGCGCCAGGATGCAGTAGACCTGTTCGCTACCCGATGGGCGCTACAGCATGGCAGGGCGCTGGAAGGAAGCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTg > 1:324383/1‑148 (MQ=255)gCTAGACCTGGGCAGGCGTTTATGGCGCCAGGATGCAGTAGACCTGTTCGCTACCCGATGGGCGCTACAGCATGGCAGGGCGCTGGAAGGAAGCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTg < 1:392653/148‑1 (MQ=255) gACCTGGGCAGGCGTTTATGGCGCCAGGATGCAGTAGACCTGTTCGCTACCCGATGGGCGCTACAGCATGGCAGGGCGCTGGAAGGAAGCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAg < 4:175798/147‑1 (MQ=255) gACCTGGGCAGGCGTTTATGGCGCCAGGATGCAGTAGACCTGTTCGCTACCCGATGGGCGCTACAGCATGGCAGGGCGCTGGAAGGAAGCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAg < 3:55588/147‑1 (MQ=255) gACCTGGGCAGGCGTTTATGGCGCCAGGATGCAGTAGACCTGTTCGCTACCCGATGGGCGCTACAGCATGGCAGGGCGCTGGAAGGAAGCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGt < 2:324383/149‑1 (MQ=255) cTGGGCAGGCGTTTATGGCGCCAGGATGCAGTAGACCTGTTCGCTACCCGATGGGCGCTACAGCATGGCAGGGCGCTGGAAGGAAGCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCAATGGCAGGtc > 1:253891/1‑146 (MQ=255) ggCAGGCGTTTATGGCGCCAGGATGCAGTAGACCTGTTCGCTACCCGATGGGCGCTACAGCATGGCAGGGCGCTGGAAGGAAGCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTGGga < 1:353581/147‑1 (MQ=255) ggCAGGCGTTTATGGCGCCAGGATGCAGTAGACCTGTTCGCTACCCGATGGGCGCTACAGCATGGCAGGGCGCTGGAAGGAAGCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCAATGGCAGGtcgagac < 2:253891/149‑7 (MQ=255) cAGGCGTTTATGGCGCCAGGATGCAGTAGACCTGTTCGCTACCCGATGGGCGCTACAGCATGGCAGGGCGCTGGAAGGAAGCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATAGCAGGTGGGAGaaa > 8:165224/1‑149 (MQ=255) gTTTATGGCGCCAGGATGCAGTAGACCTGTTCGCTACCCGATGGGCGCTACAGCATGGCAGGGCGCTGGAAGGAAGCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTGGGAGAAATGAGc > 5:392712/1‑149 (MQ=255) aTGGCGCCAGGATGCAGTAGACCTGTTCGCTACCCGATGGGCGCTACAGCATGGCAGGGCGCTGGAAGGAAGCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTc > 1:183373/1‑149 (MQ=255) gcCAGGATGCAGTAGACCTGTTCGCTACCCGATGGGCGCTACAGCATGGCAGGGCGCTGGAAGGAAGCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGt > 2:6100/1‑127 (MQ=255) gcCAGGATGCAGTAGACCTGTTCGCTACCCGATGGGCGCTACAGCATGGCAGGGCGCTGGAAGGAAGCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGt < 1:6100/127‑1 (MQ=255) ccAGGATGCAGTAGACCTGTTCGCTACCCGATGGGCGCTACAGCATGGCAGGGCGCTGGAAGGAAGCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGa > 1:148268/1‑149 (MQ=255) gATGCAGTAGACCTGTTCGCTACCCGATGGGCGCTACAGCATGGCAGGGCGCTGGAAGGAAGCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGAtct > 4:3608/1‑148 (MQ=255) aGTAGACCTGTTCGCTACCCGATGGGCGCTACAGCATGGCAGGGCGCTGGAAGGAAGCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCAATGGCAGGtcga < 1:375410/120‑4 (MQ=255) aGTAGACCTGTTCGCTACCCGATGGGCGCTACAGCATGGCAGGGCGCTGGAAGGAAGCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCAATGGCAGGtcga > 2:375410/1‑117 (MQ=255) tAGACCTGTTCGCTACCCGATGGGCGCTACAGCATGGCAGGGCGCTGGAAGGAAGCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGtt < 4:262444/149‑1 (MQ=255) aCCTGTTCGCTACCCGATGGGCGCTACAGCATGGCAGGGCGCTGGAAGGAAGCTAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTg > 4:381488/1‑149 (MQ=255) ttCGCTACCCGATGGGCGCTACAGCATGGCAGGGCGCTGGAAGGAAGCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGAt < 4:62870/147‑1 (MQ=255) gCTACCCGATGGGCGCTACAGCATGGCAGGGCGCTGGAAGGAAGCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTgg < 8:267861/106‑1 (MQ=255) gCTACCCGATGGGCGCTACAGCATGGCAGGGCGCTGGAAGGAAGCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTgg > 7:267861/1‑106 (MQ=255) cTACCCGATGGGCGCTACAGCATGGCAGGGCGCTGGAAGGAAGCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGAc < 7:131812/149‑1 (MQ=255) tACCCGATGGGCGCTACAGCATGGCAGGGCGCTGGAAGGAAGCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGa > 8:320699/1‑125 (MQ=255) tACCCGATGGGCGCTACAGCATGGCAGGGCGCTGGAAGGAAGCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGa < 7:320699/125‑1 (MQ=255) cccGATGGGCGCTACAGCATGGCAGGGCGCTGGAAGGAAGCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGACCtt > 3:343694/1‑149 (MQ=255) cccGATGGGCGCTACAGCATGGCAGGGCGCTGGAAGGAAGCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGACCtt < 3:3608/149‑1 (MQ=255) ccGATGGGCGCTACAGCATGGCAGGGCGCTGGAAGGAAGCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGACCTTg < 6:392712/149‑1 (MQ=255) gATGGGCGCTACAGCATGGCAGGGCGCTGGAAGGAAGCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGACCTTGca > 4:256815/1‑149 (MQ=255) ggCGCTACAGCATGGCAGGGCGCTGGAAGGAAGCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGGCCTTGCACAAg > 5:338308/1‑149 (MQ=255) cAGCATGGCAGGGCGCTGGAAGGAAGCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGACCTTGCACAAGCAGAgg < 4:343694/148‑1 (MQ=255) cATGGCAGGGCGCTGGAAGGAAGCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGACCTTGCACAAGCAGAGGCtgc > 2:332269/1‑149 (MQ=255) gcTGGAAGGAAGCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTGTGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGACCTTGCACAAGCAGAGGCTGC‑‑GGTGCGGGATg > 8:219024/1‑149 (MQ=255) tGGAAGGAAGCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGACCTTGCACAAGCAGAGGCTGC‑‑GGTGCGGGATg > 7:28209/1‑147 (MQ=255) aggaagCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGACCtt > 3:247084/1‑115 (MQ=255) aggaagCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGACCtt < 4:247084/115‑1 (MQ=255) aggaagCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGACCTTGCACAAGCAGAGGCTGC‑‑GGTGCGGGATGCAGaaa < 2:148268/149‑1 (MQ=255) agCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGACCTTGCACAAGCAGAGGCTGC‑‑GGTGTGGGATGCAGAAAcccc > 1:254892/1‑149 (MQ=255) gCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGGCCTTGCACAAGCAGAGGCTGC‑‑GGTGCGGGATGCAGAAACCCCg < 6:338308/149‑1 (MQ=255) gCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGACCTTGCACAAGCAGAGGCTGC‑‑GGTGCGGGATGCAGAAACCCCg > 1:205186/1‑149 (MQ=255) ggggTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGACCTTCCACAAGCAGAGGCTGC‑‑GGTGCGGGATGCAGAAACCCCGGAAGAgg < 7:165224/149‑1 (MQ=255) ggTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGACCTTGc < 1:18301/103‑1 (MQ=255) ggTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGACCTTGc > 2:18301/1‑103 (MQ=255) cAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGACCTTGCACAAGCAGAGGCTGC‑‑GGTGCGGGATGCAGAAACCCCGGAAGAGGTTGCCCGCTCCa > 3:102171/1‑149 (MQ=255) ggATCGGGCTGAGGGAGTGTCTCGACCTGCCATTg < 5:318845/35‑1 (MQ=255) ggATCGGGCTGAGGGAGTGTCTCGACCTGCCATTg > 6:318845/1‑35 (MQ=255) gATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGACCTTGCACAAGCAGAGGCTGC‑‑GGTGCGGGATGCAGAAACCCCGGAAGAGGTTGCCCGCTCCATgg > 8:315792/1‑149 (MQ=255) aTCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGACCTTGCACAAGCAGAGGCTGC‑‑GGTGCGGGATGCAGAAACCCCGGAAGAGGTTGCCCGCTCCATGGt < 1:332269/149‑1 (MQ=255) ggCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGACCTTGCACAAGCAGAGGCTGCCTGGTAAGCGATCAGGAGACCCCGGAGGAGGTTGACCCCTCAATgttg > 1:135940/1‑145 (MQ=255) tGAGGGAGTGTCTCGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGACCTTGCACAAGCAGAGGCTGC‑‑GGTGCGGGATGCAGAAACCCCGGAAGAGGTTGCCCGCTCCATGGTTGACCGt < 2:205186/149‑1 (MQ=255) aGGGAGTGTCTCGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGACCTTGCACAAGCAGAGGCTGC‑‑GGTGTGGGATGCAGAAACCCCGGAAGAGGTTGCCCGCTCCATGGTTGACCGt < 2:254892/147‑1 (MQ=255) gggAGTGTCTCGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGACCTTGCACAAGCAGAGGCTGC‑‑GGTGCGGGATGCAGAAACCCCGGAAGAGGTTGCCCGCTCCATGGTTGACCGTGaa < 4:102171/149‑1 (MQ=255) tctcGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGACCTTg > 4:224076/1‑71 (MQ=255) tctcGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGACCTTg < 3:224076/71‑1 (MQ=255) tctcGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGACCTTGCACAAGCAGAGGCTGC‑‑GGTGCGGGATGCAGAAACCCCGGAAGAGGTTGCCCGCTCCATGGTTGACCGTGAATTCTgg > 6:311248/1‑148 (MQ=255) tctcGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGACCTTGCACAAGCAGAGGCTGC‑‑GGTGCGGGATGCAGAAACCCCGGAAGAGGTTGCCCGCTCCATGGTTGACCGTGAATTCTgg < 3:388491/148‑1 (MQ=255) gACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGACCTTGCACAAGCAGAGGCTGC‑‑GGTGCGGGATGCAGAAACCCCGGAAGAGGTTGCCCGCTCCATGGTTGACCGTGAATTCTg < 7:91468/143‑1 (MQ=255) gACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGACCTTGCACAAGCAGAGGCTGC‑‑GGTGCGGGATGCAGAAACCCCGGAAGAGGTTGCCCGCTCCATGGTTGACCGTGAATTCTg > 8:91468/1‑143 (MQ=255) cTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGACCTTGCACAAGCAGAGGCTGC‑‑GGTGCGGGATGCAGAAACCCCGGAAGAGGTTGCCCGCTCCATGGTTGACCGTGAATTCTGGCAGGCGCa > 7:388081/1‑149 (MQ=255) cTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGACCTTGCACAAGCAGAGGCTGC‑‑GGTGCGGGATGCAGAAACCCCGGAAGAGGTTGCCCGCTCCATGGTTGACCGTGAATTCTGGCAGGCGCa > 1:19873/1‑149 (MQ=255) ccATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGACCTTGCACAAGCAGAGGCTGC‑‑GGTGCGGGATGCAGAAACCCCGGTAGAGGTTGCCCGCTCCATGGTTGACCGTGAATTCTGGCAGGCGCATc < 3:256815/148‑1 (MQ=255) | GCTAGACCTGGGCAGGCGTTTATGGCGCCAGGATGCAGTAGACCTGTTCGCTACCCGATGGGCGCTACAGCATGGCAGGGCGCTGGAAGGAAGCGAGGTGGGGTTGGCTTTCAGGATCGGGCTGAGGGAGTGTCTCGACCTGCCATTGCAGGTGGGAGAAATGAGCTTTCTTGCGATCTCAGGCGTTCTGGATGCGGACCTTGCACAAGCAGAGGCTGC‑‑GGTGCGGGATGCAGAAACCCCGGAAGAGGTTGCCCGCTCCATGGTTGACCGTGAATTCTGGCAGGCGCATC > NC_002947/2741605‑2741894 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |