Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,743,758	G→A	8.8%	V41V (GTG→GTA) ‡	PP_2398 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,743,758	0	G	A	8.8%	154.3 / 4.0	57	V41V (GTG→GTA) ‡	PP_2398	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (23/28);  new base A (3/2);  total (26/31)
Fisher's exact test for biased strand distribution p-value = 6.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01

GCGTTGTGCCTGTTGGAGAACTTTTATGTTTCCTGCGATGTTGAACGTTGGCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCTTGAGCAGACCCGGCAGTTTCTCGACCGATACCAG  >  NC_002947/2743611‑2743902                                                                                                                                                    |                                                                                                                                                 gCGTTGTGCCTGTTGGAGAACTTTTATGTTTCCTGCGATGTTGAACGTTGGCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTgg                                                                                                                                                 >  1:210210/1‑149 (MQ=255)  cGTTGTGCCTGTTGGAGAACTTTTATGTTTCCTGCGATGTTGAACGTTGGCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTggg                                                                                                                                                <  4:226624/149‑1 (MQ=255)          cTGTTGGAGAACTTTTATGTTTCCTGCGATGTTGAACGTTGGCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGcc                                                                                                                                         >  5:192096/1‑148 (MQ=255)            gTTGGAGAACTTTTATGTTTCCTGCGATGTTGAACGTTGGCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTAt                                                                                                                                      >  6:40266/1‑149 (MQ=255)             ttGGAGAACTTTTATGTTTCCTGCGATGTTGAACGTTGGCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTAt                                                                                                                                      <  5:158581/148‑1 (MQ=255)               ggAGAACTTTTATGTTTCCTGCGATGTTGAACGTTGGCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATgg                                                                                                                                    >  1:181012/1‑148 (MQ=255)                gagaACTTTTATGTTTCCTGCGATGTTGAACGTTGGCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGc                                                                                                                                   >  8:363407/1‑148 (MQ=255)                 ctaaCTTTTTTGTTTCCTGCGATGTTGAACGTTGGCCTAGGCGTGCGTCGCGGCCTGTTGCCGGAACTGCGGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGGGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCaa                                                                                                                                 <  6:39934/147‑1 (MQ=255)                  gaACTTTTATGTTTCCTGCGATGTTGAACGTTGGCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGTGTGCAGTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGTCAAg                                                                                                                                <  5:288310/149‑1 (MQ=255)                       tttATGTTTCCTGCGATGTTGAACGTTGGCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCtt                                                                                                                           >  4:284488/1‑149 (MQ=255)                        ttATGTTTCCTGCGATGTTGAACGTTGGCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCtt                                                                                                                           <  7:183286/148‑1 (MQ=255)                        ttATGTTTCCTGCGATGTTGAACGTTGGCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCATAGGCGCCGCCCACGGCaatccag                                                                                                                            >  6:416537/1‑142 (MQ=255)                        ttATGTTTCCTGCGATGTTGAACGTTGGCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCATAGGCGCCGCCCACGGCaatccag                                                                                                                            <  5:416537/147‑6 (MQ=255)                         tATGTTTCCTGCGATGTTGAACGTTGGCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTg                                                                                                                          <  5:40266/148‑1 (MQ=255)                             tttCCTGCGATGTTGAACGTTGGCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGcc                                                                                                                       >  4:368791/1‑147 (MQ=255)                               tCCTGCGATGTTGAACGTTGGCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCTGTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCtt                                                                                                                           >  1:212686/1‑141 (MQ=255)                               tCCTGCGATGTTGAACGTTGGCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCTGTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCtt                                                                                                                           <  2:212686/141‑1 (MQ=255)                                      aTGTTGAACGTTGGCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGa                                                                                                            <  3:368791/149‑1 (MQ=255)                                           gAACGTTGGCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAg                                                                                                           >  5:273208/1‑145 (MQ=255)                                             aCGTTGGCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCttt                                                                                                     <  3:189391/149‑1 (MQ=255)                                              cGTTGGCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTg                                                                                                    <  3:284488/149‑1 (MQ=255)                                              cGTTGGCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTg                                                                                                    <  6:192096/149‑1 (MQ=255)                                                ttGGCTTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCt                                                                                                  <  8:154969/149‑1 (MQ=255)                                                   gCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTc                                                                                               <  5:26387/149‑1 (MQ=255)                                                               gTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATggg                                                                                   <  3:356682/149‑1 (MQ=255)                                                                       cTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCttt                                                                           <  2:76395/149‑1 (MQ=255)                                                                          ttGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTa                                                                          <  4:295415/147‑1 (MQ=255)                                                                              cGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTg                                                                    >  4:329650/1‑149 (MQ=255)                                                                               ggAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCGCCCGAAAACTGGATTGACGAGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTgg                                                                   >  5:288922/1‑149 (MQ=255)                                                                                gAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTggg                                                                  >  3:103969/1‑149 (MQ=255)                                                                                       tgGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTCCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGt                                                           >  5:358856/1‑149 (MQ=255)                                                                                           cATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGccc                                                       >  4:326868/1‑149 (MQ=255)                                                                                            aTGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCCTTGCTGTCACCTGTCATgg                                                                                    <  6:22240/119‑1 (MQ=255)                                                                                            aTGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCCTTGCTGTCACCTGTCATgg                                                                                    >  5:22240/1‑119 (MQ=255)                                                                                               gAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGc                                                    <  7:363407/148‑1 (MQ=255)                                                                                                  gCGGGTGCAGTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGa                                                <  1:388864/149‑1 (MQ=255)                                                                                                           gTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCATTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCg                                                     <  8:100381/135‑1 (MQ=255)                                                                                                           gTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCg                                                     >  7:100381/1‑135 (MQ=255)                                                                                                             ggACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTcc                                     >  4:11796/1‑149 (MQ=255)                                                                                                               aCTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCtt                                   <  3:326868/149‑1 (MQ=255)                                                                                                                 tttCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTCCTGTCCCCTGTAATGGGTTGTCTTTATCGCTGGGTGGCAGAGCCCCGCTg                                                  >  6:409006/1‑132 (MQ=255)                                                                                                                   tCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCTTGa                                 <  3:11796/147‑1 (MQ=255)                                                                                                                      ggAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCTTGAGCAGa                            >  4:369022/1‑149 (MQ=255)                                                                                                                       gAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCtt                                   >  5:123597/1‑141 (MQ=255)                                                                                                                       gAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCtt                                   <  6:123597/141‑1 (MQ=255)                                                                                                                       gAGTGTGCGCCGGAAAACTGGATTGCCGTAGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCTTGAGCAGAc                           >  8:61002/1‑149 (MQ=255)                                                                                                                            tgCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCTTGAGCAGACCCgg                       <  4:103969/148‑1 (MQ=255)                                                                                                                              cgcCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCTTGAGCAGACCCGGCAg                    >  2:279924/1‑149 (MQ=255)                                                                                                                               gcCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCt                                                   <  7:362197/117‑1 (MQ=255)                                                                                                                               gcCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATAGCTGGGTGGCAGTGCCCCGCt                                                   >  8:362197/1‑117 (MQ=255)                                                                                                                                    aaaaCTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCTTGAGCAGACCCGGCAGTTtctc              <  6:358856/149‑1 (MQ=255)                                                                                                                                       aCTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCTTGAGCAGACCCGGCAGTTTCTCGAc           <  3:329650/149‑1 (MQ=255)                                                                                                                                           gATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCTTGAGCAGAccc                         <  7:22033/131‑1 (MQ=255)                                                                                                                                           gATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCTTGAGCAGAccc                         >  8:22033/1‑131 (MQ=255)                                                                                                                                           gATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCTTGAGCAGACCCGGCAGTTTCTCGACCGAt       <  2:210210/149‑1 (MQ=255)                                                                                                                                           ccttGCCATAGGCGCCGCCCACGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCTTGAGCAGACCCGGCAGTTtctc              <  1:348533/140‑1 (MQ=255)                                                                                                                                           ccttGCCATAGGCGCCGCCCACGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCTTGAGCAGACCCGGCAGTTtctc              >  2:348533/3‑142 (MQ=255)                                                                                                                                                tcGTTGGCGGCGACTATGGCAAGGTCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCTTGAGCAGACCCGGCAGTTTCTCGACCGATACCAg  <  6:288922/148‑1 (MQ=255)                                                                                                                                                ccGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCTTGAGCAGACCCGGCAGTTTCTCGACCGATACCAg  >  1:408089/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                 GCGTTGTGCCTGTTGGAGAACTTTTATGTTTCCTGCGATGTTGAACGTTGGCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCGCCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCTTGAGCAGACCCGGCAGTTTCTCGACCGATACCAG  >  NC_002947/2743611‑2743902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑