Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,756,837	A→T	9.6%	intergenic (+16/+17)	czcA‑II → / ← PP_2411	cation efflux system protein/MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,756,837	0	A	T	9.6%	142.2 / 6.1	52	intergenic (+16/+17)	czcA‑II/PP_2411	cation efflux system protein/MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (22/25);  new base T (2/3);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01

CGGGGCAGAGGTGCAGCGGCCACTGGCGACGGTGGTGATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTCATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACGCCACCAGCGCAATCACCACCAGGTAGAAC  >  NC_002947/2756689‑2756983                                                                                                                                                       |                                                                                                                                                   cgggGCTGAGGTGCAGCGGCCATTGGCGACGGTGGTGATTGGCGGAATCTTGTGTTCTACCGCG‑‑CTGACGTTGCTGTTGTTGCCGTCGTTGTATCTGTGTGCGTATCTGCGGGATGAGCAGCAGGAATGCTGAGCATTTGGGGCTGCaa                                                                                                                                                    <  1:304005/149‑1 (MQ=37) cgggGCAGAGGTGCAGCGGCCACTGGCGACGGTGGTGATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCaa                                                                                                                                                    <  5:110011/149‑1 (MQ=255) cgggGCAGAGGTGCAGCGGCCACTGGCGACGGTGGTGATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCaa                                                                                                                                                    <  5:301933/149‑1 (MQ=255)   gggCAGAGGTGCAGCGGCCACTGGCGACGGTGGTGATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAgg                                                                                                                                                  <  6:146913/149‑1 (MQ=255)            tGCAGCGGCCACTGGCGACGGTGGTGATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCcaa                                                                                                                                         <  6:349183/149‑1 (MQ=255)             gCAGCGGCCACTGGCGACGGTGGTGATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCcaa                                                                                                                                         >  5:419742/1‑148 (MQ=255)             gCAGCGGCCACTGGCGACGGTGGTGATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCcaa                                                                                                                                         >  6:271312/1‑148 (MQ=255)             gCAGCGGCCACTGGCGACGGTGGTGATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCcaa                                                                                                                                         >  5:425498/1‑148 (MQ=255)              cAGCGGCCACTGGCGACGGTGGTGATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCCTTGCAGCCCcaaat                                                                                                                                       <  6:109906/149‑3 (MQ=255)               aGCGGCCACTGGCGAGGGTGGTGATTGGCGGAATCTTGACTTCTCCGGTGTAGTTACGTTGCTGGTGTTGCGGGCGTTGTATCAGTGGCCGGATCGGCGCGATGAGCAGCAGGACGGCTGAGCATTTGGGGCTGCAAGGCAGCCCcaac                                                                                                                                        <  1:131446/149‑1 (MQ=255)                  ggCCACTGGCGACGGTGGTGATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCt                                                                                                                                   <  3:387803/149‑1 (MQ=255)                      aCTGGCGACGGTGGTGATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCaaa                                                                                                                               <  2:275459/149‑1 (MQ=255)                        tGGCGACGGTGGTGATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACg                                                                                                                             >  3:88111/1‑149 (MQ=255)                        tGGCGACGGTGGTGATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACg                                                                                                                             >  8:99509/1‑149 (MQ=255)                          gCGACGGTGGTGATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACg                                                                                                                             <  6:419742/147‑1 (MQ=255)                          gCGACGGTGGTGATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACg                                                                                                                             <  6:425498/147‑1 (MQ=255)                          gCGACGGTGGTGATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGc                                                                                                                           >  3:220519/1‑149 (MQ=255)                                gtggtgATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCg                                                                                                                     <  4:88111/149‑1 (MQ=255)                                 tggtgATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCCTTGCAGCCCcaaa                                                                                                                                        >  4:20198/1‑128 (MQ=255)                                 tggtgATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCCTTGCAGCCCcaaa                                                                                                                                        <  3:20198/129‑2 (MQ=255)                                   gtgATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGc                                                                                                                  <  8:10046/149‑1 (MQ=255)                                    tgATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCa                                                                                                                 <  5:271312/149‑1 (MQ=255)                                     gATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCGAACGGCCCTTCGGGctg                                                                                                                >  1:222421/1‑147 (MQ=255)                                                    gTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCa                                                                                                                                                <  4:224668/102‑1 (MQ=255)                                                    gTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCa                                                                                                                                                >  3:224668/1‑102 (MQ=255)                                                    gTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGt                                                                                                 >  5:154066/1‑149 (MQ=255)                                                     tcttctACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTTCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGt                                                                                                 <  5:323853/148‑1 (MQ=255)                                                     tcttctACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTc                                                                                                <  3:111482/149‑1 (MQ=255)                                                       ttctACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCca                                                                                              >  7:63806/1‑149 (MQ=255)                                                       ttctACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCca                                                                                              <  7:359517/149‑1 (MQ=255)                                                          tACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCg                                                                                                                     >  5:195902/1‑123 (MQ=255)                                                          tACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCg                                                                                                                     <  6:195902/123‑1 (MQ=255)                                                           aCCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGcc                                                                                                                          >  5:188013/1‑117 (MQ=255)                                                           aCCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGcc                                                                                                                          <  6:188013/117‑1 (MQ=255)                                                                   cTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGgtc                                                                                    <  7:99509/149‑1 (MQ=255)                                                                       cGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTCa                                                                                <  1:143773/149‑1 (MQ=255)                                                                        gTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCCCCCCCAGGTCGGCAt                                                                               >  3:174780/1‑149 (MQ=255)                                                                        gTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTCAt                                                                               <  6:425161/149‑1 (MQ=255)                                                                        gTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTCAt                                                                               <  2:3229/149‑1 (MQ=255)                                                                                  ttGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGATGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCACAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCAAACACACGTCGTCAAGCAAAGAAAt                                                                     >  1:297929/1‑149 (MQ=255)                                                                                      cGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTCATGCAAAGAAATCcgc                                                                 >  6:290999/1‑149 (MQ=255)                                                                                               aTCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACTCAGGTCGTCATGCAAAGAAATCCGCGACGTTTcc                                                        >  7:352032/1‑149 (MQ=255)                                                                                                tCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTCATGCAAAGAAATCCGCGACGTTTcc                                                        <  4:220519/148‑1 (MQ=255)                                                                                                          tATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTCATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCaa                                             <  5:290999/149‑1 (MQ=255)                                                                                                                                       aGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTTATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACGCCACCAGCGCAATc                 <  8:63806/148‑1 (MQ=255)                                                                                                                                           tttGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTCATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACGCCACCAGCGCAATcaccac            >  7:195120/1‑149 (MQ=255)                                                                                                                                           tttGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTCATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACGCCACCAGCGCAATcaccac            >  5:364036/1‑149 (MQ=255)                                                                                                                                            ttGGGGCTGCCTTGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTCATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACGCCACCAgc                       <  6:125191/137‑1 (MQ=255)                                                                                                                                              ggggCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTCATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACGCCACCAGCGCAATCACCACCAg         >  4:206633/1‑149 (MQ=255)                                                                                                                                               gggCTGCCTTGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTCATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACGCCACCAGCGCAATCACCACCAgg        >  8:359734/1‑149 (MQ=255)                                                                                                                                               gggCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTCATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACACCACCAGCGCAATCACCACCAgg        <  1:359227/149‑1 (MQ=255)                                                                                                                                                ggCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTCATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACGCCACCAGCGCAATCACCACCAGGt       <  3:206633/149‑1 (MQ=255)                                                                                                                                                 gCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTCATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACGCCACCAGCGCAATCACCACCAgg        >  6:377803/1‑147 (MQ=255)                                                                                                                                                     cAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTCATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACGCCACCAGCGCAATCACCACCAGGTAGAAc  >  2:102168/1‑149 (MQ=255)                                                                                                                                                     cAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTCATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACGCCACCAGCGCAATCACCACCAGGTAGAAc  >  3:231630/1‑149 (MQ=255)                                                                                                                                                       |                                                                                                                                                   CGGGGCAGAGGTGCAGCGGCCACTGGCGACGGTGGTGATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTCATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACGCCACCAGCGCAATCACCACCAGGTAGAAC  >  NC_002947/2756689‑2756983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑