Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,756,838 G→T 9.8% intergenic (+17/+16) czcA‑II → / ← PP_2411 cation efflux system protein/MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,756,8380GT 9.8% 154.4 / 6.6 51intergenic (+17/+16)czcA‑II/PP_2411cation efflux system protein/MFS transporter
Reads supporting (aligned to +/- strand):  ref base G (22/24);  new base T (2/3);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.64e-01

GGGCAGAGGTGCAGCGGCCACTGGCGACGGTGGTGATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTCATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACGCCACCAGCGCAATCACCACCAGGTAGAAC  >  NC_002947/2756691‑2756983
                                                                                                                                                     |                                                                                                                                                 
gggCAGAGGTGCAGCGGCCACTGGCGACGGTGGTGATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAgg                                                                                                                                                  <  6:146913/149‑1 (MQ=255)
         tGCAGCGGCCACTGGCGACGGTGGTGATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCcaa                                                                                                                                         <  6:349183/149‑1 (MQ=255)
          gCAGCGGCCACTGGCGACGGTGGTGATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCcaa                                                                                                                                         >  5:425498/1‑148 (MQ=255)
          gCAGCGGCCACTGGCGACGGTGGTGATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCcaa                                                                                                                                         >  5:419742/1‑148 (MQ=255)
          gCAGCGGCCACTGGCGACGGTGGTGATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCcaa                                                                                                                                         >  6:271312/1‑148 (MQ=255)
           cAGCGGCCACTGGCGACGGTGGTGATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCCTTGCAGCCCcaaat                                                                                                                                       <  6:109906/149‑3 (MQ=255)
            aGCGGCCACTGGCGAGGGTGGTGATTGGCGGAATCTTGACTTCTCCGGTGTAGTTACGTTGCTGGTGTTGCGGGCGTTGTATCAGTGGCCGGATCGGCGCGATGAGCAGCAGGACGGCTGAGCATTTGGGGCTGCAAGGCAGCCCcaac                                                                                                                                        <  1:131446/149‑1 (MQ=255)
               ggCCACTGGCGACGGTGGTGATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCt                                                                                                                                   <  3:387803/149‑1 (MQ=255)
                   aCTGGCGACGGTGGTGATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCaaa                                                                                                                               <  2:275459/149‑1 (MQ=255)
                     tGGCGACGGTGGTGATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACg                                                                                                                             >  8:99509/1‑149 (MQ=255)
                     tGGCGACGGTGGTGATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACg                                                                                                                             >  3:88111/1‑149 (MQ=255)
                       gCGACGGTGGTGATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACg                                                                                                                             <  6:425498/147‑1 (MQ=255)
                       gCGACGGTGGTGATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACg                                                                                                                             <  6:419742/147‑1 (MQ=255)
                       gCGACGGTGGTGATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGc                                                                                                                           >  3:220519/1‑149 (MQ=255)
                             gtggtgATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCg                                                                                                                     <  4:88111/149‑1 (MQ=255)
                              tggtgATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCCTTGCAGCCCcaaa                                                                                                                                        <  3:20198/129‑2 (MQ=255)
                              tggtgATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCCTTGCAGCCCcaaa                                                                                                                                        >  4:20198/1‑128 (MQ=255)
                                gtgATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGc                                                                                                                  <  8:10046/149‑1 (MQ=255)
                                 tgATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCa                                                                                                                 <  5:271312/149‑1 (MQ=255)
                                  gATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCGAACGGCCCTTCGGGctg                                                                                                                >  1:222421/1‑147 (MQ=255)
                                                 gTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCa                                                                                                                                                >  3:224668/1‑102 (MQ=255)
                                                 gTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCa                                                                                                                                                <  4:224668/102‑1 (MQ=255)
                                                 gTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGt                                                                                                 >  5:154066/1‑149 (MQ=255)
                                                  tcttctACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTTCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGt                                                                                                 <  5:323853/148‑1 (MQ=255)
                                                  tcttctACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTc                                                                                                <  3:111482/149‑1 (MQ=255)
                                                    ttctACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCca                                                                                              <  7:359517/149‑1 (MQ=255)
                                                    ttctACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCca                                                                                              >  7:63806/1‑149 (MQ=255)
                                                       tACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCg                                                                                                                     >  5:195902/1‑123 (MQ=255)
                                                       tACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCg                                                                                                                     <  6:195902/123‑1 (MQ=255)
                                                        aCCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGcc                                                                                                                          >  5:188013/1‑117 (MQ=255)
                                                        aCCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGcc                                                                                                                          <  6:188013/117‑1 (MQ=255)
                                                                cTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGgtc                                                                                    <  7:99509/149‑1 (MQ=255)
                                                                    cGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTCa                                                                                <  1:143773/149‑1 (MQ=255)
                                                                     gTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCCCCCCCAGGTCGGCAt                                                                               >  3:174780/1‑149 (MQ=255)
                                                                     gTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTCAt                                                                               <  2:3229/149‑1 (MQ=255)
                                                                     gTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTCAt                                                                               <  6:425161/149‑1 (MQ=255)
                                                                               ttGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGATGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCACAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCAAACACACGTCGTCAAGCAAAGAAAt                                                                     >  1:297929/1‑149 (MQ=255)
                                                                                   cGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTCATGCAAAGAAATCcgc                                                                 >  6:290999/1‑149 (MQ=255)
                                                                                            aTCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACTCAGGTCGTCATGCAAAGAAATCCGCGACGTTTcc                                                        >  7:352032/1‑149 (MQ=255)
                                                                                             tCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTCATGCAAAGAAATCCGCGACGTTTcc                                                        <  4:220519/148‑1 (MQ=255)
                                                                                                       tATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTCATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCaa                                             <  5:290999/149‑1 (MQ=255)
                                                                                                                                    aGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTTATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACGCCACCAGCGCAATc                 <  8:63806/148‑1 (MQ=255)
                                                                                                                                        tttGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTCATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACGCCACCAGCGCAATcaccac            >  5:364036/1‑149 (MQ=255)
                                                                                                                                        tttGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTCATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACGCCACCAGCGCAATcaccac            >  7:195120/1‑149 (MQ=255)
                                                                                                                                         ttGGGGCTGCCTTGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTCATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACGCCACCAgc                       <  6:125191/137‑1 (MQ=255)
                                                                                                                                           ggggCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTCATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACGCCACCAGCGCAATCACCACCAg         >  4:206633/1‑149 (MQ=255)
                                                                                                                                            gggCTGCCTTGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTCATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACGCCACCAGCGCAATCACCACCAgg        >  8:359734/1‑149 (MQ=255)
                                                                                                                                            gggCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTCATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACACCACCAGCGCAATCACCACCAgg        <  1:359227/149‑1 (MQ=255)
                                                                                                                                             ggCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTCATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACGCCACCAGCGCAATCACCACCAGGt       <  3:206633/149‑1 (MQ=255)
                                                                                                                                              gCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTCATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACGCCACCAGCGCAATCACCACCAgg        >  6:377803/1‑147 (MQ=255)
                                                                                                                                                  cAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTCATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACGCCACCAGCGCAATCACCACCAGGTAGAAc  >  3:231630/1‑149 (MQ=255)
                                                                                                                                                  cAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTCATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACGCCACCAGCGCAATCACCACCAGGTAGAAc  >  2:102168/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
GGGCAGAGGTGCAGCGGCCACTGGCGACGGTGGTGATTGGCGGAATCTTGTCTTCTACCGCG‑‑CTGACGTTGCTGGTGTTGCCGGCGTTGTATCAGTGGGCGTATCGGCGGGATGAGCAGCAGGAAGGCTGAGCATTTGGGGCTGCAAGGCAGCCCCAACAACCTCAAACGGCCCTTCGGGCACCAGCCCGCACACCGTCCACACCCAGGTCGTCATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACGCCACCAGCGCAATCACCACCAGGTAGAAC  >  NC_002947/2756691‑2756983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑