Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,780,240 C→T 7.2% A5V (GCC→GTC)  PP_2433 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,780,2400CT 7.2% 161.1 / 2.2 55A5V (GCC→GTC) PP_2433hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (23/28);  new base T (2/2);  total (25/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAAGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCCTGCAGGCGCTGCTGGACATTCCCGAGCTTGCCAGCGATTTCGAAATCCACACCCGCATCACCGATGGTTTTGCCGCCACC  >  NC_002947/2780093‑2780388
                                                                                                                                                   |                                                                                                                                                    
gCAAGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGcc                                                                                                                                                     >  1:398573/1‑149 (MQ=255)
 cAAGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGcc                                                                                                                                                     >  3:66455/1‑148 (MQ=255)
         cACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGa                                                                                                                                             <  8:74974/148‑1 (MQ=255)
         cACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTAGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGa                                                                                                                                             <  1:276720/148‑1 (MQ=255)
          aCCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGGCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAg                                                                                                                                            <  6:410414/148‑1 (MQ=255)
             ttCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGcatg                                                                                                                                        >  2:229892/1‑149 (MQ=255)
               ccTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGcatgc                                                                                                                                       <  8:194227/148‑1 (MQ=255)
                ctctGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGcatgcc                                                                                                                                      <  4:66455/148‑1 (MQ=255)
                   tGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGcatgcc                                                                                                                                      <  5:15220/145‑1 (MQ=255)
                   tGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGcatgcc                                                                                                                                      >  6:15220/1‑145 (MQ=255)
                       gTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAgg                                                                                                                                              >  7:181627/1‑133 (MQ=255)
                       gTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAgg                                                                                                                                              <  8:181627/133‑1 (MQ=255)
                                gCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGTCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCt                                                                                                                     >  8:332969/1‑149 (MQ=255)
                                   gCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGTCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTcg                                                                                                                  <  7:332969/149‑1 (MQ=255)
                                         cGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGcc                                                                                                                             <  4:314022/132‑1 (MQ=255)
                                         cGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGcc                                                                                                                             >  3:314022/1‑132 (MQ=255)
                                          ggCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTCGCGACAAc                                                                                                           >  3:290363/1‑149 (MQ=255)
                                                ggCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTCGCGACAACGGTTAc                                                                                                     >  3:317666/1‑149 (MQ=255)
                                                 gCAATGCTGGCGGTCAACCTGGCAATGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGTCGAAAAGGAGCATGCCATGCtt                                                                                                                                <  5:204446/121‑1 (MQ=255)
                                                 gCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGTCGAAAAGGAGCATGCCATGCtt                                                                                                                                >  6:204446/1‑121 (MQ=255)
                                                 gCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTCGCGACAACGGTTAcc                                                                                                    <  5:9944/149‑1 (MQ=255)
                                                 gCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTCGCGACAACGGTTAcc                                                                                                    <  5:160652/149‑1 (MQ=255)
                                                 gCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTCGCGACAACGGTTAc                                                                                                     >  6:177611/1‑148 (MQ=255)
                                                 gCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTCGCGACAACGGTTAc                                                                                                     <  5:303220/148‑1 (MQ=255)
                                                 gCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCATGCCGAAGTGCTTCGCGACAACGGTTAc                                                                                                     <  1:229892/148‑1 (MQ=255)
                                                        tGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTCGCGCCAACGGTTACCACGAGTa                                                                                             >  7:232941/1‑149 (MQ=255)
                                                            gttCAACCTGGCAAAGCCCTTGCTTCGACCGCCCGACCAGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAATAGGAGCATGCCATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCgg                                                                                         <  8:232941/147‑1 (MQ=255)
                                                            ggTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGcatgccatgc                                                                                                                                  <  6:34833/108‑1 (MQ=255)
                                                            ggTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGcatgccatgc                                                                                                                                  >  5:34833/1‑108 (MQ=255)
                                                            ggTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCgg                                                                                         >  4:60815/1‑149 (MQ=255)
                                                            ggTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCgg                                                                                         >  2:131190/1‑149 (MQ=255)
                                                             gTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCggg                                                                                        <  8:352679/149‑1 (MQ=255)
                                                                aaCCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGgcgc                                                                                     >  2:167948/1‑149 (MQ=255)
                                                                      gCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCCTg                                                                                <  4:290363/148‑1 (MQ=255)
                                                                      gCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCCTGc                                                                               <  5:177611/149‑1 (MQ=255)
                                                                       cAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCCTGc                                                                               <  4:228406/148‑1 (MQ=255)
                                                                       cAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCCTGc                                                                               >  3:228406/1‑148 (MQ=255)
                                                                        aaaGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGaa                                                                                                                          >  1:203268/1‑104 (MQ=255)
                                                                        aaaGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGaa                                                                                                                          <  2:203268/104‑1 (MQ=255)
                                                                            cccTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCCTGCAGgcgc                                                                         <  2:398573/149‑1 (MQ=255)
                                                                                   ttCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCAAATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATACCATGCTTGCCGAATTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCCTGCAGGCGCTGCTGGa                                                                  >  8:123098/1‑149 (MQ=255)
                                                                                          gCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGTCATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCCTGCAGGCGctgctg                                                                    <  1:272817/140‑1 (MQ=255)
                                                                                          gCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGTCATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCCTGCAGGCGctgctg                                                                    >  2:272817/1‑140 (MQ=255)
                                                                                                cccGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCCTGCAGGCGCTGCTGGACATTCCCGAGCtt                                                     <  1:167948/149‑1 (MQ=255)
                                                                                                    gCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCCTGCAGGCGCTGCTGGACATTCCCGAGCTTGc                                                   >  3:126365/1‑147 (MQ=255)
                                                                                                      aaaCTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCCTGCAGGCGCTGCTGGACATTCCCGAGCTTGCCAGc                                               >  4:85124/1‑149 (MQ=255)
                                                                                                          tGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCCTGCAGGCGCTGCTGGACATTCCCGAGCTTGCCAGCGAtt                                           >  7:363070/1‑149 (MQ=255)
                                                                                                                tGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCCTGCAGGCGCTGCTGGACATTCCCGAGCTTGCCAGCGATTTCGaaa                                     <  4:399419/149‑1 (MQ=255)
                                                                                                                  aCCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCCTGCAGGCGCTGCTGGACATTCCCGAGCTTGCCAGCGATTTCGAAAt                                    <  3:60815/148‑1 (MQ=255)
                                                                                                                       gCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCCTGCAGGCGCTGCTGGACATTCCCTAGCTTGCCAGCGATTTCGAAATCCacac                              <  4:394214/149‑1 (MQ=255)
                                                                                                                       gCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCCTGCAGGCGCTGCTGGACATTCCCGAGCTTGCCa                                                 >  2:207499/1‑130 (MQ=255)
                                                                                                                       gCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCCTGCAGGCGCTGCTGGACATTCCCGAGCTTGCCa                                                 <  1:207499/130‑1 (MQ=255)
                                                                                                                       gCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCCTGCAGGCGCTGCTGGACATTCCCGAGCTTGCCAGCGATTTCGAAATCCacac                              >  1:202061/1‑149 (MQ=255)
                                                                                                                                       tGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCCTGCAGGCGCTGCTGGACATTCCCGAGCTTGCCAGCGATTTCGAAATCAACACCCGCATCACCGATGGt              >  7:263622/1‑149 (MQ=255)
                                                                                                                                         tCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCCTGCAGGCGCTGCTGGACATTCCCGa                                                         >  3:397847/1‑104 (MQ=255)
                                                                                                                                         tCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCCTGCAGGCGCTGCTGGACATTCCCGa                                                         <  4:397847/104‑1 (MQ=255)
                                                                                                                                            gCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCCTGCAGGCGCTGCTGGACATTCCCGAGCTTGCCAGCGATTTCGAAATCCACACCCGCATCACCGATGGTTTTgc         <  8:363070/149‑1 (MQ=255)
                                                                                                                                                   ccGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCCTGCAGGCGCTGCTGGACATTCCCGAGCTTGCCAGCGATTTCGAAATCCACACCCGCATCACCGATGGTTTTGCCGCCAcc  >  6:22355/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCAAGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCCTGCAGGCGCTGCTGGACATTCCCGAGCTTGCCAGCGATTTCGAAATCCACACCCGCATCACCGATGGTTTTGCCGCCACC  >  NC_002947/2780093‑2780388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑