Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,802,568 A→T 7.7% S318C (AGC→TGC)  rbsA‑I → ribose ABC transporter ‑ ATP‑binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,802,5680AT 7.7% 156.8 / 3.1 52S318C (AGC→TGC) rbsA‑Iribose ABC transporter ‑ ATP‑binding subunit
Reads supporting (aligned to +/- strand):  ref base A (31/17);  new base T (2/2);  total (33/19)
Fisher's exact test for biased strand distribution p-value = 6.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

CTCAAGGTCGACAAACTGTGCCGTGGCGACAAGGTGCGCGAGGTGTCGTTCGAAGTCAGGGCAGGGGAGATTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCCTGCTGTTGACGCAGTCGATCAGCGCCAACATTGCGTT  >  NC_002947/2802421‑2802716
                                                                                                                                                   |                                                                                                                                                    
ctCAAGGTCGACAAACTGTGCCGTGGCGACAAGGTGCGCGAGGTGTCGTTCGAAGTCAGGGCAGGGGAGATTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATTTACGGTGCCGATCGCGCCGACAg                                                                                                                                                     >  3:181512/1‑149 (MQ=255)
 tCAAGGTCGACAAACTGTGCCGTGGCGACAAGGTGCGCGAGGTGTCGTTCGAAGTCAGGGCAGGGGAGATTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAgc                                                                                                                                                    >  1:45717/1‑149 (MQ=255)
     ggTCGACAAACTGTGCCGTGGCGACAAGGTGCGCGAGGTGTCGTTCGAAGTCAGGGCAGGGGAGATTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAgcggcg                                                                                                                                                >  4:135468/1‑149 (MQ=255)
      gTCGACAAACTGTGCCGTGGCGACAAGGTGCGCGAGGTGTCGTTCGAAGTCAGGGCAGGGGAGATTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGcggcgg                                                                                                                                               <  1:171168/149‑1 (MQ=255)
                    ccGTGGCGACAAGGTGCGCGAGGTGTCGTTCGAAGTCAGGGCAGGGGAAATTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGc                                                                                                                                  >  1:303534/1‑148 (MQ=255)
                           gACAAGGTGCGCGAGGTGTCGTTCGAAGTCAGGGCAGGGGAGATTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAgccgc                                                                                                                           >  5:42499/1‑148 (MQ=255)
                              aaGGTGCGCGAGGTGTCGTTCGAAGTCAGGGCAGGGGAGATTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCcaca                                                                                                                       <  6:216565/149‑1 (MQ=255)
                                       gAGGTGTCGTTCGAAGTCAGGGCAGGGGAGATTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAg                                                                                                              <  7:318524/149‑1 (MQ=255)
                                             tCGTTCGAAGTCAGGGCAGGGGAGATTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGa                                                                                                        >  3:23349/1‑149 (MQ=255)
                                                ttCGAAGTCAGGGCAGGGGAGATTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTc                                                                                                     >  8:242180/1‑149 (MQ=255)
                                                   gAAGTCAGGGCAGGGGAGATTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGc                                                                                                   <  3:135468/148‑1 (MQ=255)
                                                      gTCAGGGCAGGGGAGATTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGccc                                                                                                 >  4:275302/1‑147 (MQ=255)
                                                      gTCAGGGCAGGGGAGATTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCaa                                                                                               <  2:45717/149‑1 (MQ=255)
                                                                  gagaTTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATc                                                                                                          >  2:206563/1‑126 (MQ=255)
                                                                  gagaTTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATc                                                                                                          <  1:206563/126‑1 (MQ=255)
                                                                            gCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATc                                                                         >  1:46881/1‑149 (MQ=255)
                                                                              aTCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATcgc                                                                       >  5:56631/1‑149 (MQ=255)
                                                                                   cGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCgcgc                                                                     <  8:47270/146‑1 (MQ=255)
                                                                                   cGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGCGCGTGCCGGTATCGCGCTGa                                                                  >  1:270706/1‑149 (MQ=255)
                                                                                     gCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCg                                                                                                    >  3:74944/1‑113 (MQ=255)
                                                                                     gCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCg                                                                                                    <  4:74944/113‑1 (MQ=255)
                                                                                     gCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGAt                                                                 >  6:134759/1‑148 (MQ=255)
                                                                                      ccTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCg                                                                              <  3:22309/134‑1 (MQ=255)
                                                                                      ccTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCg                                                                              >  4:22309/1‑134 (MQ=255)
                                                                                        tGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCt                                                                    <  6:131328/142‑1 (MQ=255)
                                                                                        tGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCt                                                                    >  5:131328/1‑142 (MQ=255)
                                                                                        tGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAAcc                                                             >  1:298795/1‑149 (MQ=255)
                                                                                              gcgcCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGa                                                                  <  3:234458/138‑1 (MQ=255)
                                                                                              gcgcCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGa                                                                  >  4:234458/1‑138 (MQ=255)
                                                                                                   ggccgTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCGCAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCa                                                   >  7:82506/1‑148 (MQ=255)
                                                                                                           cGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAgg                                          >  3:385722/1‑149 (MQ=255)
                                                                                                           cGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAgg                                          <  2:303534/149‑1 (MQ=255)
                                                                                                           cGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAgg                                          >  5:46757/1‑149 (MQ=255)
                                                                                                             aGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGc                                        >  4:206208/1‑149 (MQ=255)
                                                                                                              gctgctGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGcc                                       <  6:42499/149‑1 (MQ=255)
                                                                                                                   tgcGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCctgctg                                  >  5:4189/1‑149 (MQ=255)
                                                                                                                    gcgcCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCCTGCTGt                                 >  3:321094/1‑149 (MQ=255)
                                                                                                                          gATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCCTGCTGTTGACg                            >  2:12718/1‑148 (MQ=255)
                                                                                                                            tCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCCTGCTGTTGACGCa                          <  6:56631/148‑1 (MQ=255)
                                                                                                                             cTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCCTGCTGTTGACGCAGt                        >  6:225871/1‑149 (MQ=255)
                                                                                                                              tACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCCTGCTGTTGACGCAGTc                       >  4:120964/1‑149 (MQ=255)
                                                                                                                               aCGGTGCCGATCGCGCCGACTGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCt                                                                    <  5:227307/103‑1 (MQ=255)
                                                                                                                               aCGGTGCCGATCGCGCCGACTGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCt                                                                    <  5:221597/103‑1 (MQ=255)
                                                                                                                               aCGGTGCCGATCGCGCCGACTGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCt                                                                    >  6:221597/1‑103 (MQ=255)
                                                                                                                               aCGGTGCCGATCGCGCCGACTGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCt                                                                    >  6:227307/1‑103 (MQ=255)
                                                                                                                                cGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAATGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCCTGCTGTTGACGCa                          >  4:157340/1‑144 (MQ=255)
                                                                                                                                cGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCCTGCTGTTGACGCa                          <  3:157340/144‑1 (MQ=255)
                                                                                                                                cGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCCTGCTGTTGACGCAGTCGa                     <  6:46757/149‑1 (MQ=255)
                                                                                                                                 ggTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCCTGCTGTTGACGCAGTCGAt                    >  6:72582/1‑149 (MQ=255)
                                                                                                                                   tGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCCTGCTGTTGACGCAGTCGAt                    >  8:15137/1‑147 (MQ=255)
                                                                                                                                           gcgcCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCCTGCTGTTGAc                             >  3:70870/1‑130 (MQ=255)
                                                                                                                                           gcgcCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCCTGCTGTTGAc                             <  4:70870/130‑1 (MQ=255)
                                                                                                                                                   aGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCCTGCTGTTGAc                             >  2:3403/1‑122 (MQ=255)
                                                                                                                                                   aGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCCTGCTGTTGAc                             <  1:3403/122‑1 (MQ=255)
                                                                                                                                                   aGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCCTGCTGTTGACGCAGTCGATCAGCGCCAACATTGCGtt  <  7:15137/149‑1 (MQ=255)
                                                                                                                                                   aGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCCTGCTGTTGACGCAGTCGATCAGCGCCAACATTGCGtt  <  2:46881/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CTCAAGGTCGACAAACTGTGCCGTGGCGACAAGGTGCGCGAGGTGTCGTTCGAAGTCAGGGCAGGGGAGATTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCCTGCTGTTGACGCAGTCGATCAGCGCCAACATTGCGTT  >  NC_002947/2802421‑2802716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑