Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,862,971 C→T 9.0% Q11* (CAA→TAA)  gllR → HTH‑type transcriptional regulator GalR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,862,9710CT 9.0% 122.8 / 2.4 45Q11* (CAA→TAA) gllRHTH‑type transcriptional regulator GalR
Reads supporting (aligned to +/- strand):  ref base C (21/19);  new base T (2/2);  total (24/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

GCCTGTGCAGGGCACTAATGCGTTCATTTGCAACGGCTATGCGTTTTATTTATTGCCGGGCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTCACCCGCGCCATCGCCGAATTGGAGGCGCGCTTTGCCGTGCCCCTGTTCGAGCGCCA  >  NC_002947/2862826‑2863110
                                                                                                                                                 |                                                                                                                                           
gCCTGTGCAGGGCACTAATGCGTTCATTTGCAACGGCTATGCGTTTTATTTATTGCCGGGCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAg                                                                                                                                          >  1:193482/1‑149 (MQ=255)
        aGGGCACTAATGCGTTCATTTGCAACGGCTATGCGTTTTATTTATTGCCGGGCAAGCGGGGTAGAGGCATAGTCGCAGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCcgcgcg                                                                                                                                  >  8:299902/1‑149 (MQ=255)
         gggCACTAATGCGTTCATTTGCAACGGCTATGCGTTTTATTTATTGCCGGGCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGt                                                                                                                                 >  6:128072/1‑149 (MQ=255)
               tAATGCGTTCATTTGCAACGGCTATGCGTTTTATTTATTGCCGGGCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATcc                                                                                                                           >  4:284241/1‑149 (MQ=255)
                             gCAACGGCTATGCGTTTTATTTATTGCCGGGCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTc                                                                                                                               <  4:143437/131‑1 (MQ=255)
                             gCAACGGCTATGCGTTTTATTTATTGCCGGGCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTc                                                                                                                               >  3:143437/1‑131 (MQ=255)
                              cAACGGCTATGCGTTTTATTTATTGCCGGGCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTgg                                                                                                            <  1:389694/149‑1 (MQ=255)
                                          gTTTTATTTATTGCCGGGCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGAAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTcgc                                                                                                >  2:321395/1‑149 (MQ=255)
                                           ttttATTTATTGCCGGGCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTcgcg                                                                                               <  8:185979/149‑1 (MQ=255)
                                             ttatttatTGCCGGGCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTgg                                                                                                            <  5:411587/134‑1 (MQ=255)
                                             ttatttatTGCCGGGCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTgg                                                                                                            >  6:411587/1‑134 (MQ=255)
                                             ttatttatTGCCGGGCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCgg                                                                                              <  3:284241/148‑1 (MQ=255)
                                                  tattGCCGGGCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCgg                                                                                              <  6:277223/143‑1 (MQ=255)
                                                  tattGCCGGGCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCgg                                                                                              >  5:277223/1‑143 (MQ=255)
                                                   attGCCGGGCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTgg                                                                                                            <  5:109958/128‑1 (MQ=255)
                                                   attGCCGGGCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTgg                                                                                                            >  6:109958/1‑128 (MQ=255)
                                                    ttGCCGGGCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACg                                                                                        >  6:338693/1‑147 (MQ=255)
                                                     tGCCGGGCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAg                                                                                     >  3:56566/1‑149 (MQ=255)
                                                       ccGGGCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCGGAGCGCCGGGAGCCCTTATTATCGATGTCGAACTGCCCAACCTGATGCAAGTCCTCTCGTTCATCCTGGTAGCCGAGCTGGGCAGCGTTTc                                                                                                  <  5:25467/134‑1 (MQ=255)
                                                       ccGGGCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGt                                                                                   <  1:321395/149‑1 (MQ=255)
                                                       ccGGGCAAGCGGGGTAGAGGCATAGTAGCCGCAGTCACGAGACGAGCGCCGGGAGCCCTAATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCCTTCATCCGGGTAGCAGAGCTGGGCAGCGTTTc                                                                                                  >  6:25467/1‑134 (MQ=255)
                                                             aaGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGtt                                                                             <  2:390966/149‑1 (MQ=255)
                                                             aaGCGGGGCAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCGCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGtt                                                                             <  1:351509/149‑1 (MQ=255)
                                                               gCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAg                                                                                                        <  3:118861/120‑1 (MQ=255)
                                                               gCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAg                                                                                                        >  4:118861/1‑120 (MQ=255)
                                                                      agagGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATcc                                                                                                                           <  6:398663/94‑1 (MQ=255)
                                                                      agagGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATcc                                                                                                                           >  5:398663/1‑94 (MQ=255)
                                                                       gagGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCa                                                                    <  7:299902/148‑1 (MQ=255)
                                                                          gCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTcc                                                                >  3:152851/1‑149 (MQ=255)
                                                                                   ccgcAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGTAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTCAcc                                                       >  7:77770/1‑149 (MQ=255)
                                                                                   ccgcAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGTAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTCAcc                                                       >  7:83440/1‑149 (MQ=255)
                                                                                   ccgcAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTCAcc                                                       >  4:164662/1‑149 (MQ=255)
                                                                                              agccgagcGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTCACCCGCGCCATCGc                                            >  1:114196/1‑149 (MQ=255)
                                                                                               gccgagcGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTCACCCGCGCCATCGcc                                           <  5:128072/149‑1 (MQ=255)
                                                                                                  gagcGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTc                                                          >  7:349414/1‑131 (MQ=255)
                                                                                                  gagcGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTc                                                          <  8:349414/131‑1 (MQ=255)
                                                                                                       ccGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTCACCCGCGCCATCGCCGAATTGGa                                   <  4:152851/149‑1 (MQ=255)
                                                                                                                cTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTCACCCGCGCCATCGCCGAATTGGAGGCGCGCtt                          >  6:374487/1‑149 (MQ=255)
                                                                                                                cTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTCACCCGCGCCATCGCCGAATTGGAGGCGCGCtt                          >  8:269401/1‑149 (MQ=255)
                                                                                                                   aTGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTCACCCGCGCCATCGCCGAATTGGAGGCGCGCTTTGc                       >  1:404380/1‑149 (MQ=255)
                                                                                                                         gATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTCACCCGCGCCATCGCCGAATTGGAGGCGCGCTTTGCCGTGc                  <  4:56566/148‑1 (MQ=255)
                                                                                                                         gATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTCACCCGCGCCATCGCCGAATTGGAGGCGCGCTTTGCCGTGc                  <  2:193482/148‑1 (MQ=255)
                                                                                                                               gAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTCACCCGCGCCATCGCCGAATTGGAGGCGCGCTTTGACGTGCCCCTGtt           >  5:115464/1‑149 (MQ=255)
                                                                                                                                        aaCCTGATGTAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTCACCCGCGCCATCGCCGAATTGGAGGCGCGCTTTGCCGTGCCCCTGTTCGAGCGCCa  <  8:77770/149‑1 (MQ=255)
                                                                                                                                        aaCCTGATGTAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTCACCCGCGCCATCGCCGAATTGGAGGCGCGCTTTGCCGTGCCCCTGTTCGAGCGCCa  <  8:83440/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                           
GCCTGTGCAGGGCACTAATGCGTTCATTTGCAACGGCTATGCGTTTTATTTATTGCCGGGCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTCACCCGCGCCATCGCCGAATTGGAGGCGCGCTTTGCCGTGCCCCTGTTCGAGCGCCA  >  NC_002947/2862826‑2863110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑