Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,873,647 G→A 8.3% V143M (GTG→ATG) ‡ cigR → membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,873,6470GA 8.3% 136.6 / 2.1 48V143M (GTG→ATG) ‡cigRmembrane protein
Reads supporting (aligned to +/- strand):  ref base G (22/22);  new base A (2/2);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.55e-01

TCCAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGG‑CATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGCC  >  NC_002947/2873502‑2873786
                                                                                                                                                 |                                                                                                                                            
tCCAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTgg                                                                                                                                           >  1:4173/1‑149 (MQ=255)
tCCAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTgg                                                                                                                                           >  7:162539/1‑149 (MQ=255)
tCCAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTgg                                                                                                                                           >  3:404932/1‑149 (MQ=255)
   aGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGa                                                                                                                                        <  3:169524/149‑1 (MQ=255)
   aGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATCCCGGTCGCCACGGCGa                                                                                                                                        >  8:307978/1‑149 (MQ=255)
          cAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATCCCGGTCGCCACGGCGAccagaat                                                                                                                                 >  1:75020/1‑144 (MQ=255)
           agaagaACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGat                                                                                                                                 >  5:319085/1‑148 (MQ=255)
           agaagaACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATCCCGGTCGCCACGGCGAccagaat                                                                                                                                 <  7:307978/148‑6 (MQ=255)
                aaCCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATCCCGGTCGCCACGGCGACCAGAatcaggtc                                                                                                                           <  2:75020/149‑5 (MQ=255)
                 aCCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTAt                                                                                                                          <  2:4173/149‑1 (MQ=255)
                 aCCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTAt                                                                                                                          <  3:311818/149‑1 (MQ=255)
                   cTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTAt                                                                                                                          >  8:388332/1‑147 (MQ=255)
                   cTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGa                                                                                                                        <  7:153623/149‑1 (MQ=255)
                        ccGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGGGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGgtgt                                                                                                                   >  1:335079/1‑149 (MQ=255)
                        ccGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTAt                                                                                                                          <  4:127803/142‑1 (MQ=255)
                        ccGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTAt                                                                                                                          >  3:127803/1‑142 (MQ=255)
                           cgGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGa                                                                                                                <  3:386977/149‑1 (MQ=255)
                              cAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACg                                                                                                             <  6:319085/149‑1 (MQ=255)
                                   cccTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGG‑Ca                                                                                                       >  2:203140/1‑149 (MQ=255)
                                    ccTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGG‑CAt                                                                                                      >  4:29986/1‑149 (MQ=255)
                                        ccACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGG‑CATTCGa                                                                                                  <  1:203140/149‑1 (MQ=255)
                                           ccTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGG‑CATTCGACt                                                                                                >  3:216669/1‑148 (MQ=255)
                                                    gCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGG‑CATTCGACTAAGGCCGCAg                                                                                      <  4:404932/149‑1 (MQ=255)
                                                           agCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGG‑CATTCGACTAAGGCCGCAGGTGCGAg                                                                               <  2:335079/149‑1 (MQ=255)
                                                                    gtcgtcTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGG‑CATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCt                                                                      <  8:162539/149‑1 (MQ=255)
                                                                     tcgtcTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGG‑CATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTc                                                                     >  3:46929/1‑149 (MQ=255)
                                                                       gtcTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGG‑CATTCGACTAAGGCCGCAg                                                                                      <  5:54168/130‑1 (MQ=255)
                                                                       gtcTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGG‑CATTCGACTAAGGCCGCAg                                                                                      >  6:54168/1‑130 (MQ=255)
                                                                        tcTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGG‑CATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGt                                                                  >  4:212583/1‑149 (MQ=255)
                                                                               gggCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGG‑CATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGt                                                                  >  5:309321/1‑142 (MQ=255)
                                                                               gggCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGG‑CATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGt                                                                  <  6:309321/142‑1 (MQ=255)
                                                                                      tGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGa                                                                                                                        <  1:49914/82‑1 (MQ=255)
                                                                                      tGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGa                                                                                                                        >  2:49914/1‑82 (MQ=255)
                                                                                        ccTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGG‑CATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTc                                                                 >  4:247885/1‑134 (MQ=255)
                                                                                        ccTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGG‑CATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTc                                                                 <  3:247885/134‑1 (MQ=255)
                                                                                        ccTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGG‑CATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGc                                                  >  6:88965/1‑149 (MQ=255)
                                                                                         cTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGatcat                                                                                                                              >  7:377011/1‑73 (MQ=255)
                                                                                         cTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGatcat                                                                                                                              <  8:377011/73‑1 (MQ=255)
                                                                                          tCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGG‑CATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGt                                                >  5:180466/1‑149 (MQ=255)
                                                                                                 gacgGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGG‑CATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGc                                          >  4:137323/1‑148 (MQ=255)
                                                                                                      gTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGG‑CATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGTTCCCTGCAGGCGCTGGCGTGCCTGCGATggg                                    <  7:388332/149‑1 (MQ=255)
                                                                                                          gAGTGGATGCAATCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGATGTGTTGAACGGGGCCATTCGACTAAGGCCGCAGGTGCGAGCAg                                                                            <  1:232690/106‑1 (MQ=255)
                                                                                                          gAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGG‑CATTCGACTAAGGCCGCAGGTGCGAGCAg                                                                            >  2:232690/1‑105 (MQ=255)
                                                                                                                aTGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGG‑CATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCgcagca                          <  5:88965/149‑1 (MQ=255)
                                                                                                                           cgcAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGG‑CATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCACTGGCGTGCCTGCGTTGGGCTGCTGCGCAGCCCATCgcag               <  4:216669/149‑4 (MQ=255)
                                                                                                                            gcAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGG‑CATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCTGCGCAGCCCATCgcagg              <  4:46929/149‑5 (MQ=255)
                                                                                                                                 ccTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGG‑CATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCaa         >  7:61049/1‑149 (MQ=255)
                                                                                                                                           ggcGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGG‑CATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGcc  <  3:341072/144‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                            
TCCAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGG‑CATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGCC  >  NC_002947/2873502‑2873786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑