Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,919,018 G→A 7.2% A2680T (GCT→ACT)  PP_2561 → hemolysin‑type calcium‑binding bacteriocin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,919,0180GA 7.2% 164.1 / 2.2 55A2680T (GCT→ACT) PP_2561hemolysin‑type calcium‑binding bacteriocin
Reads supporting (aligned to +/- strand):  ref base G (25/26);  new base A (2/2);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

TTCTTCGATGTGCCACCGGTACCGGGTTCGGGCGCTTCGACGCTGGTGCGGTTCGACATCATGGAAGGCCTGTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTGGCCGGCCTGCTGGCGGCCGGAGCGACCTTCTATGACGGCGGCAACATCATCCTCGGCGGCAGCGGCAGCGACTTGCTCGAAGGCCGGGGTGGCGACGATATC  >  NC_002947/2918877‑2919164
                                                                                                                                             |                                                                                                                                                  
ttcttcGATGTGCCACCGGTACCGGGTTCGGGCGCTTCGACGCTGGTGCGGTTCGACATCATGGAAGGCCTGTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCgg                                                                                                                                             >  8:24411/1‑149 (MQ=255)
      gATGTGCCACCGGTACCGGGTTCGGGCGCTTCGACGCTGGTGCGGTTCGACATCATGGAAGGCCTGTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCg                                                                                                                                        <  4:163125/148‑1 (MQ=255)
       aTGTGCCACCGGTTCCGGGTTCGGGCGCTTCGTCTCTGGTGGGGTTCGACATCATGGAAGGCCTGTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGt                                                                                                                                       <  8:117390/148‑1 (MQ=255)
           gCCACCGGTACCGGGTTCGGGCGCTTCGACGCTGGTGCGGTTCGACATCATGGAAGGCCTGTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGa                                                                                                                                  >  7:301933/1‑149 (MQ=255)
            ccACCGGTACCGGGTTCGGGCGCTTCGACGCTGGTGCGGTTCGACATCATGGAAGGCCTGTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGAc                                                                                                                                 <  5:1220/149‑1 (MQ=255)
            ccACCGGTACCGGGTTCGGGCGCTTCGACGCTGGTGCGGTTCGACATCATGGAAGGCCTGTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGAc                                                                                                                                 <  4:69208/149‑1 (MQ=255)
              aCCGGTACCGGGTTCGGGCGCTTCGACGCTGGTGCGGTTCGACATCATGGAAGGCCTGTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGAcc                                                                                                                                >  8:345830/1‑148 (MQ=255)
                       gggTTCGGGCGCTTCGACGCTGGTGCGGTTCGACATCATGGAAGGCCTGTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGcc                                                                                                                      <  5:362274/149‑1 (MQ=255)
                               gcgcTTCGACGCTGGTGCGGTTCGACATCATGGAAGGCCTGTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGAc                                                                                                              <  8:320760/149‑1 (MQ=255)
                                cgcTTCGACGCTGGTGCGGTTCGACATCATGGAAGGCCTGTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACg                                                                                                             <  2:118393/149‑1 (MQ=255)
                                  cTTCGACGCTGGTGCGGTTCGACATCATGGAAGGCCTGTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGc                                                                                                           <  8:301933/149‑1 (MQ=255)
                                   ttCGACGCTGGTGCGGTTCGACATCATGGAAGGCCTGTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGcc                                                                                                          >  1:242842/1‑149 (MQ=255)
                                      gACGCTGGTGCGGTTCGACATCATGGAAGGCCTGTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTgg                                                                                                       >  6:42411/1‑149 (MQ=255)
                                         gCTGGTGCGGTTCGACATCATGGAAGGCCTGTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTggccg                                                                                                    >  7:31710/1‑149 (MQ=255)
                                                cGGTTCGACATCATGGAACGCCTGTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTGGCCGGCctgct                                                                                             <  8:80331/149‑1 (MQ=255)
                                                      gACATCATGGAAGGCCTGTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTGGCCGGCCTGCTggcggc                                                                                       <  7:345830/149‑1 (MQ=255)
                                                       aCATCATGGAAGGCCTGTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTGGCCGGCCTGCTGGCGGcc                                                                                      <  5:65379/149‑1 (MQ=255)
                                                         atcatGGAAGGCCTGTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCa                                                                                                                               <  7:385190/106‑1 (MQ=255)
                                                         atcatGGAAGGCCTGTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCa                                                                                                                               >  8:385190/1‑106 (MQ=255)
                                                         atcatGGAAGGCCTGTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTGGCCGGCCTGCTGGCGGCCgg                                                                                    <  7:24411/149‑1 (MQ=255)
                                                         atcatGGAAGGCCTGTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTGGCCGGCCTGCTGGCGGCCg                                                                                     >  2:109710/1‑148 (MQ=255)
                                                          tcatGGAAGGCCTGTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTGGCCGGCCTGCTGGCGGCCGGa                                                                                   <  2:43819/149‑1 (MQ=255)
                                                           catGGAAGGCCTGTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTGGCCGGCCTGCTGGCGGCCGGAg                                                                                  >  7:85271/1‑149 (MQ=255)
                                                            atGGAAGGCCTGTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGc                                                                                                                       >  6:199939/1‑111 (MQ=255)
                                                            atGGAAGGCCTGTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGc                                                                                                                       <  5:199939/111‑1 (MQ=255)
                                                            atGGAAGGCCTGTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTGGCCGGCCTGCTGGCGGCCGGAGc                                                                                 >  6:155493/1‑149 (MQ=255)
                                                            atGGAAGGCCTGTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGAAAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTGGCCGGCCTGCTGGCGGCCGGAGc                                                                                 >  2:286460/1‑149 (MQ=255)
                                                               gAAGGCCTGTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTGGCCGGCCTGCTGGCGGCCGGAGCGa                                                                               <  8:31710/148‑1 (MQ=255)
                                                                     cTGTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAAc                                                                                                                             <  1:16876/96‑1 (MQ=255)
                                                                     cTGTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAAc                                                                                                                             >  2:16876/1‑96 (MQ=255)
                                                                     cTGTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTGGCCGGCCTGCTGGCGGCCGGa                                                                                   <  8:301548/138‑1 (MQ=255)
                                                                     cTGTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTGGCCGGCCTGCTGGCGGCCGGa                                                                                   >  7:301548/1‑138 (MQ=255)
                                                                     cTGTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTGGCCGGCCTGCTGGCGGCCGGAGCGACCTTCTa                                                                        >  2:106920/1‑149 (MQ=255)
                                                                      tGTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTGGCCGGCCTGCTGGCGGCCGGAGCGAc                                                                              <  8:10842/142‑1 (MQ=255)
                                                                      tGTCGGGGTCGGCGCATGGAGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTGGCCGGCCTGATGGCGGCCGGAGCGAc                                                                              >  7:10842/1‑142 (MQ=255)
                                                                       gTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAAc                                                                                                                             >  3:8775/1‑94 (MQ=255)
                                                                       gTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAAc                                                                                                                             <  4:8775/94‑1 (MQ=255)
                                                                        tCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTGGCCGGCCTGCTGGCGGCCGGAGCGACCTTCTAt                                                                       <  5:398277/147‑1 (MQ=255)
                                                                              tCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTGGCCGGCCTGCTGGCGGCCGGAGCGACCTTCTATGAcggcgg                                                               <  2:242842/149‑1 (MQ=255)
                                                                                     aTGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTGGCCGGCCTGCTGGCGGCCGGAGCGACCTTCTATGACGGCGGCAAcatc                                                        <  1:286460/149‑1 (MQ=255)
                                                                                        gCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTGGCCGGCCTGCTGGCGGCCGGAGCGACCTTCTATGACGGCGGCAAc                                                           <  6:210355/143‑1 (MQ=255)
                                                                                        gCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTGGCCGGCCTGCTGGCGGCCGGAGCGACCTTCTATGACGGCGGCAAc                                                           >  5:210355/1‑143 (MQ=255)
                                                                                             ttCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTGGCCGGCCTGCTGGCGGCCGGAGCGACCTTCTATGACGGCGGCAACATCATCCTcgg                                                >  6:124457/1‑149 (MQ=255)
                                                                                             ttCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCACTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTGGCCGGCCTGCTGGCGGCCTGAGCGACCTTCTATGACGGCGGCAACatcatc                                                     <  3:82538/144‑1 (MQ=255)
                                                                                             ttCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCACTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTGGCCGGCCTGCTGGCGGCCGGAGCGACCTTCTATGACGGCGGCAACatcatc                                                     <  3:321041/144‑1 (MQ=255)
                                                                                             ttCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCACTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTGGCCGGCCTGCTGGCGGCCGGAGCGACCTTCTATGACGGCGGCAACatcatc                                                     >  4:82538/1‑144 (MQ=255)
                                                                                             ttCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCACTACCCGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTGGCCCGCCAGCAGGCGGCCGGAGCGACCTTCTATGACGGCGGCAAcatcagc                                                     >  4:321041/1‑142 (MQ=255)
                                                                                                cTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTGGCCGGCCTGCTGGCGGCCGGAGCGACCTTCTATGACGGCGGCAACATCATCCTcggcgg                                             >  3:617/1‑149 (MQ=255)
                                                                                                        tGACAGGTAAGACGGAACCTCGCGGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTGGCCGGCCTGCTGGCGGCCGGAGCGACCTTCTATGACGGCGGCAACATCATCCTCGgcggcagcggca                                     <  6:289577/149‑1 (MQ=255)
                                                                                                            aGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTGGCCGGCCTGCTGGCGGCCGGAGCGACCTTCTATGACGGCGGCAACATCATCCTCGGCGGCAGCGGCAGCGa                                 >  2:199686/1‑149 (MQ=255)
                                                                                                              cGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTGGCCGGCCTGCTGGCGGCCGGAGCGACCTTCTATGACGGCGGCAACATCATCCTCGGCGGCAGCGGCAGCGACt                               >  3:403468/1‑149 (MQ=255)
                                                                                                                       cACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTGGCCGGCCTGCTGGCGGCCGGAGCGACCTTCTATGACGGCGGCAACATCATCCTCGGCGGCAGCGGCAGCGACTTGCTCGAAg                      >  7:153763/1‑149 (MQ=255)
                                                                                                                        aCCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTGGCCGGCCTGCTGGCGGCCGGAGCGACCTTCTATGACGGCGGCAACATCATCCTCGGCGGCAGCGGCAGCGACTTGCTCGAAgg                     >  4:387287/1‑149 (MQ=255)
                                                                                                                                 ccTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTGGCCGGCCTGCTGGCGGCCGGAGCGACCTTCTATGACGGCGGCAACATCATCCTCGGCGGCAGCGGCAGCGACTTGCTCGAAGGCCGGGGTg             <  8:85271/148‑1 (MQ=255)
                                                                                                                                   tACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTGGCCGGCCTGCTGGCGGCCGGAGCGACCTTCTATGACGGCGGCAACATCATCCTCGGCGGCAGCGGCCGCGACTTGCTCGAAGGCCGGGGAGGcg          >  2:357169/1‑149 (MQ=255)
                                                                                                                                           gcgcTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTGGCCGGCCTGCTGGCGGCCGGAGCGACCTTCTATGACGGCGGCAACATCATCCTCGGCGGCAGCGGCAGCGACTTGCTCGAAGGCCGGGGTGGCGACGATATc  >  8:101104/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                  
TTCTTCGATGTGCCACCGGTACCGGGTTCGGGCGCTTCGACGCTGGTGCGGTTCGACATCATGGAAGGCCTGTCGGGGTCGGCGCATGGCGACTTCCTGCGCGGTGACAGCGAAGACGCCACCTCGCTGCCTACTGCAGGCGCTACCGGCAGCGTGCTGACCAACATCAGCCTGATCGACGGCCTGGCCGGCCTGCTGGCGGCCGGAGCGACCTTCTATGACGGCGGCAACATCATCCTCGGCGGCAGCGGCAGCGACTTGCTCGAAGGCCGGGGTGGCGACGATATC  >  NC_002947/2918877‑2919164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑