Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,921,460 G→A 100% G3494R (GGG→AGG)  PP_2561 → hemolysin‑type calcium‑binding bacteriocin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,921,4600GA100.0% 118.2 / NA 37G3494R (GGG→AGG) PP_2561hemolysin‑type calcium‑binding bacteriocin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/14);  total (23/14)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑‑GACACCCTGATCGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAAT  >  NC_002947/2921318‑2921603
                                                                                                                                                |                                                                                                                                               
gTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑‑GACACCCTGATCagggggg                                                                                                                                           <  1:398937/149‑1 (MQ=255)
gTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCATGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑‑GCCACCCTGATCagggggg                                                                                                                                           >  3:114813/1‑149 (MQ=255)
 ttGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGACGGTAACGACACATTGTTCGGCGACGTGGGTACC‑‑GACACCCTGATCAGGGGGGc                                                                                                                                          >  8:14893/1‑149 (MQ=255)
     tGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑‑GACACCCTGATCagg                                                                                                                                               <  4:364147/140‑1 (MQ=255)
     tGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑‑GACACCCTGATCagg                                                                                                                                               >  3:364147/1‑140 (MQ=255)
              ccGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑‑GACACCCTGATCAGGGGGGCCGGCAACGACTTc                                                                                                                             <  6:295945/149‑1 (MQ=255)
                  tAACGCCAACCTGAACGGGGGCGCTGGCGATGCCAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑‑GACACACGGATCAGGGTGGCCGGCAACGACtgactc                                                                                                                          >  8:384139/1‑143 (MQ=255)
                  tAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGACGGTAACGACACATTGTTCGGCGACGTGGGTACC‑‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCa                                                                                                                         <  7:14893/149‑1 (MQ=255)
                      gACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCt                                                                                                                           <  1:355280/143‑1 (MQ=255)
                      gACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCt                                                                                                                           >  2:355280/1‑143 (MQ=255)
                      gACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACgg                                                                                                                     >  1:100084/1‑149 (MQ=255)
                      gACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACgg                                                                                                                     >  4:70123/1‑149 (MQ=255)
                           ccTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCgg                                                                                                                <  3:193017/149‑1 (MQ=255)
                           ccTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCgg                                                                                                                >  1:237065/1‑149 (MQ=255)
                                    gggCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGATCGGCGATGTGGGTACC‑‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAAc                                                                                                        >  4:135389/1‑148 (MQ=255)
                                              gATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACggtggt                                                                                             <  2:237065/149‑1 (MQ=255)
                                                    aGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTaa                                                                                                         >  8:128244/1‑131 (MQ=255)
                                                    aGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTaa                                                                                                         <  7:128244/131‑1 (MQ=255)
                                                       cTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACggtgg                                                                                              >  6:180385/1‑139 (MQ=255)
                                                       cTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACggtgg                                                                                              <  5:180385/139‑1 (MQ=255)
                                                               cggcaacggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAAc                                                                             >  7:61912/1‑148 (MQ=255)
                                                               cggcaacggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAAc                                                                             >  7:59889/1‑148 (MQ=255)
                                                                         aaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCAGGACCCCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCAt                                                                     >  1:112762/1‑148 (MQ=255)
                                                                           tGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAGCGACACCATGATCg                                                                >  5:177085/1‑149 (MQ=255)
                                                                                        ggcggcGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑‑GACACCCTGATCAGGGGGGc                                                                                                                                          >  5:64185/1‑62 (MQ=255)
                                                                                        ggcggcGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑‑GACACCCTGATCAGGGGGGc                                                                                                                                          <  6:64185/62‑1 (MQ=255)
                                                                                            gcgATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGAt                                               <  3:70123/149‑1 (MQ=255)
                                                                                                  gTAACGACACATTGTTCGGCGACGTGGGTACC‑‑GACACCCTGATCAGGGGGGCCGGCAAGGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTc                                         <  2:24284/149‑1 (MQ=255)
                                                                                                   tAACGACACATTGTTCGGCGACGTGGGTACC‑‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCAGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTc                                         >  7:393576/1‑148 (MQ=255)
                                                                                                     aCGACACATTGATCGGCGATGTGGGTACC‑‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCa                                       <  3:135389/148‑1 (MQ=255)
                                                                                                                              tACC‑‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTa                                                                               >  4:254347/1‑83 (MQ=255)
                                                                                                                              tACC‑‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTa                                                                               <  3:254347/83‑1 (MQ=255)
                                                                                                                                 c‑‑GACACCCTGATCAGGGGGGCGGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTg           >  7:201034/1‑148 (MQ=255)
                                                                                                                                    gACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGat         >  6:332922/1‑149 (MQ=255)
                                                                                                                                       acCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGAt                                                        <  7:195888/99‑1 (MQ=255)
                                                                                                                                       acCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGAt                                                        >  8:195888/1‑99 (MQ=255)
                                                                                                                                        cccTGATCAGGGGGGCCGGCAACGACTTCCTCAAGGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGatcatc     <  6:177085/149‑1 (MQ=255)
                                                                                                                                        cccTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATTATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGatcat      >  5:109093/1‑148 (MQ=255)
                                                                                                                                         ccTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGatcatc     >  5:57770/1‑148 (MQ=255)
                                                                                                                                         ccTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGatcatc     >  5:157953/1‑148 (MQ=255)
                                                                                                                                           tGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAAt  >  1:221048/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                               
GTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑‑GACACCCTGATCGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAAT  >  NC_002947/2921318‑2921603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑