Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,955,574 T→C 7.3% V266A (GTG→GCG)  PP_2587 → LuxR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,955,5740TC 7.3% 151.7 / 2.5 56V266A (GTG→GCG) PP_2587LuxR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (28/23);  new base C (2/2);  total (31/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01

CTCGACCAAGTCGCTGGCCGAGCGGTTGGGAGTGTCGGAGGACACCATCAAGTCGCACCGCAAGAACGTCTACGCCAAGCTCGACATCGGCACCCAGTCCGAACTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCTGC‑AACCCCACCGCAAAGGAAAACCAACAATG‑AACGCGCCTTTCGCCCCGCAACGCCAGACCCGTGACTACCAGGCAGCCGAT  >  NC_002947/2955428‑2955716
                                                                                                                                                  |                                                                                                                                                
cTCGACCAAGTCGCTGGCCGAGCGGTTGGGAGTGTCGGAGGACACCATCAAGTCGCACCGCAAGAACGTCTACGCCAAGCTCGACATCGGCACCCAGTCCGAACTGTTCTTCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGc                                                                                                                                                <  7:339714/149‑1 (MQ=255)
   ttccAAGTCGCTGGCCGAGCGGTTGGGTGTGTCGGAGGACACCATCACTTCGCACCGCAAGAACGTCTACGCCAAGCTCGACATCGGCACCCAGTCCGAACTTTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGAGTggt                                                                                                                                               <  4:86247/145‑3 (MQ=255)
   gACCAAGGCGCTGGCCGAGCGGTTGGGAGTGTCGGAGGACACCATCAAGTCGCACCGCAAGAACGTCTACGCCAAGCTCGACATCGGCACCCAGTCCGAACTGTTCTCCCTGCTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCt                                                                                                                                               <  4:367034/147‑1 (MQ=255)
    aCCAAGTCGCTGGCCGAGCGGTTGGGAGTGTCGGAGGACACCATCAAGTCGCACCGCAAGAACGTCTACGCCAAGCTCGACATCGGCACCCAGTCCGAACTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTggg                                                                                                                                            >  1:334020/1‑149 (MQ=255)
      cAAGTCGCTGGCCGAGCGGTTGGGAGTGTCGGAGGACACCATCAAGTCGCACCGCAAGAACGTCTACGCCAAGCTCGACATCGGCACCCAGTCCGAACTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGc                                                                                                                                           >  8:30287/1‑148 (MQ=255)
      cAAGTCGCTGGCCGAGCGGTTGGGAGTGTCGGAGGACACCATCAAGTCGCACCGCAAGAACGTCTACGCCAAGCTCGACATCGGCACCCAGTCCGAACTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGCACGCCCTGGGCa                                                                                                                                          >  5:118343/1‑149 (MQ=255)
         gTCGCTGGCCGAGCGGTTGGGAGTGTCGGAGGACACCATCAAGTCGCACCGCAAGAACGTCTACGCCAAGCTCGACATCGGCACCCAGTCCGAACTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAgg                                                                                                                                       <  7:43681/149‑1 (MQ=255)
         gTCGCTGGCCGAGCGGTTGGGAGTGTCGGAGGACACCATCAAGTCGCACCGCAAGAACGTCTACGCCAAGCTCGACATCGGCACCCAGTCCGAACTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGCACGCCCTGGGCattg                                                                                                                                       <  6:118343/149‑4 (MQ=255)
            gCTGGCCGAGCGGTTGGGAGTGTCGGAGGACACCATCAAGTCGCACCGCAAGAACGTCTACGCCAAGCTCGACATCGGCACCCAGTCCGAACTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGAc                                                                                                                                     <  7:30287/148‑1 (MQ=255)
                gCCGAGCGGTTGGGAGTGTCGGAGGACACCATCAAGTCGCACCGCAAGAACGTCTACGCCAAGCTCGACATCGGCACCCAGTCCGAACTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCTGGGCGTGCTGGGCAAGGACCCGc                                                                                                                                 <  2:334020/148‑1 (MQ=255)
                    aGCGGTTGGGAGTGTCGGAGGACACCATCAAGTCGCACCGCAAGAACGTCTACGCCAAGCTCGACATCGGCACCCAGTCCGAACTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGa                                                                                                                             >  8:144124/1‑148 (MQ=255)
                             gAGTGTCGGAGGACACCATCAAGTCGCACCGCAAGAACGTCTACGCCAAGCTCGACATCGGCACCCAGTCCGAACTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATg                                                                                                                   <  7:144124/149‑1 (MQ=255)
                                  tCGGAGGACACCATCAAGTCGCACCGCAAGAACGTCTACGCCAAGCTCGACATCGGCACCCAGTCCGAACTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGc                                                                                                                >  5:351349/1‑147 (MQ=255)
                                        gaCACCATCAAGTCGCACCGCAAGAACGTCTACGCCAAGCTCGACATCGGCACCCAGTCCGAACTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTgcg                                                                                                        >  5:414868/1‑149 (MQ=255)
                                        gaCACCATCAAGTCGCACCGCAAGAACGTCTACGCCAAGCTCGACATCGGCACCCAGTCCGAACTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGCACGCCCTGGGCattg                                                                                                                                       <  3:353377/118‑4 (MQ=255)
                                        gaCACCATCAAGTCGCACCGCAAGAACGTCTACGCCAAGCTCGACATCGGCACCCAGTCCGAACTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGCACGCCCTGGGCattg                                                                                                                                       >  4:353377/1‑115 (MQ=255)
                                           acCATCAAGTCGCACCGCAACAACGTCAACGCCAAGCTAGACATCGGCACCCAGTCCGAAATGATATCCCTGTTCATCGATGCGCTGGCCAAAGCCCAGGGCGAGCTGGGCAAGGACCCCCTGGAAAGCTAAATGGGAAATCTGCGCt                                                                                                      >  8:369812/1‑148 (MQ=255)
                                             cATCAAGTCGCACCGCAAGAACGTCTACGCCAAGCTCGACATCGGCACCCAGTCCGAACTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGaa                                                                                                   <  6:414868/149‑1 (MQ=255)
                                             cATCAAGTCGCACCGCAAGAACGTCTACGCCAAGCTCGACATCGGCACCCAGTCCGAACTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGaa                                                                                                   >  8:288031/1‑149 (MQ=255)
                                              aTCAAGTCGCACCGCAAGAACGTGAACGCAAAGCTCGACATCGGCACCCAGTCCGTACTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAAc                                                                                                  <  7:369812/149‑1 (MQ=255)
                                                   gTCGCACCGCAAGAACGTCTACGCCAAGCTCGACATCGGCACCCAGTCCGAACTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAg                                                                                             >  2:391048/1‑149 (MQ=255)
                                                          cGCAAGAACGTCTACGCCAAGCTCGACATCGGCACCCAGTCCGAACTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCt                                                                                      <  6:351349/149‑1 (MQ=255)
                                                           gCAAGAACGTCTACGCCAAGCTCGACATCGGCACCCAGTCCGAACTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCTg                                                                                     >  1:153291/1‑149 (MQ=255)
                                                             aaGAACGTCTACGCCAAGCTCGACATCGGCACCCAGTCCGAACTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCTGc                                                                                    >  5:121251/1‑148 (MQ=255)
                                                                        cGCCAAGCTCGACATCGGCACCCAGTCCGAACTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCTGC‑AACCCCAcc                                                                          <  7:371246/146‑1 (MQ=255)
                                                                           cAAGCTCGACATCGGCACCCAGTCCGAACTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCTGC‑AACCCCACCGCAAAg                                                                    >  7:20258/1‑149 (MQ=255)
                                                                                tCGACATCGGCACCCAGTCCGAACTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCTGC‑AACCCCACCGCAAAGGaaaa                                                               >  7:385711/1‑149 (MQ=255)
                                                                                tCGACATCGGCACCCAGTCCGAACTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCTGC‑AACCCCACCGCAAAGGaaaa                                                               >  2:101009/1‑149 (MQ=255)
                                                                                tCGACATCGGCACCCAGTCCGAACTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCTGC‑AACCCCACCGCAAAGGaaaa                                                               >  6:211815/1‑149 (MQ=255)
                                                                                  gACATCGGCACCCAGTCCGAACTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCTGC‑AACCCCACCGCAAAGGAAAAc                                                              <  8:385711/148‑1 (MQ=255)
                                                                                      tCGGCACCCAGTCCGAACTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCTGC‑AACCCCACCGCAAAGGAAAACcaaca                                                         <  6:226947/149‑1 (MQ=255)
                                                                                         gCACCCAGTCCGAACTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCTGC‑AACCCCACCGCAAAGGAAAACCAACAAt                                                       >  5:173788/1‑148 (MQ=255)
                                                                                            cccAGTCCGAACTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCTGC‑AACCCCACCGCAAAGGAAAACCAACAATG‑AAc                                                  <  1:391048/149‑1 (MQ=255)
                                                                                             ccAGTCCGAACTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCTGC‑AACCCCACCGCAAAGGAAAACCAACAATG‑AAcg                                                 >  4:229978/1‑149 (MQ=255)
                                                                                                gTCCGAACTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCTGC‑AACCCCACCGCAAAGGAAAACCAACAATG‑AAc                                                  <  4:136507/145‑1 (MQ=255)
                                                                                                gTCCGAACTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCTGC‑AACCCCACCGCAAAGGAAAACCAACAATG‑AAc                                                  >  3:136507/1‑145 (MQ=255)
                                                                                                   cGAACTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCTGC‑AACCCCACCGCAAAGGAAAACCAACAATG‑AACGCGCCtt                                           >  8:148078/1‑149 (MQ=255)
                                                                                                    gAACTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCTGC‑AACCCCACCGCAAAGGAAAACCAACAATG‑AACGCGCCttt                                          >  3:28426/1‑149 (MQ=255)
                                                                                                     aaCTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCTGC‑AACCCCAcc                                                                          >  5:178532/1‑117 (MQ=255)
                                                                                                     aaCTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCTGC‑AACCCCAcc                                                                          <  6:178532/117‑1 (MQ=255)
                                                                                                             tcCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCTGC‑AACCCCACCGCAAAGGaaaa                                                               >  5:203712/1‑120 (MQ=255)
                                                                                                             tcCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCTGC‑AACCCCACCGCAAAGGaaaa                                                               <  6:203712/120‑1 (MQ=255)
                                                                                                             tcCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCTGC‑AACCCCACCGCAAAGGAAAACCAACAATG‑AACGCGCCTTTCGCCCCGc                                  >  8:98159/1‑148 (MQ=255)
                                                                                                               ccTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCTGC‑AACCCCACCGCAAAGGAAAACCAACAATG‑AACGCGCCTTTCGCCCCGCAAc                               >  1:88222/1‑149 (MQ=255)
                                                                                                                 tGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCTGC‑AACCCCACCGCAAAGGAAAACCAACAATG‑AACGCGCCTTTCGcccc                                    >  2:176799/1‑142 (MQ=255)
                                                                                                                 tGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCTGC‑AACCCCACCGCAAAGGAAAACCAACAATG‑AACGCGCCTTTCGcccc                                    <  1:176799/142‑1 (MQ=255)
                                                                                                                    tCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCTGC‑AACCCCACCGCAAAGGAAAACCAACAATG‑AACGCGCCTTTCGCCCCGCAACGCCa                           <  7:288031/148‑1 (MQ=255)
                                                                                                                          aTGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCTGC‑AACCCCACCGCAAAGGAAAACCAACAATG‑AACGCGCCTTTCGCCCCGCAACGCCAGACCCGt                    <  7:98159/149‑1 (MQ=255)
                                                                                                                             cgcTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCTGC‑AACCCCACCGCAAAGGAAAACCAACAATG‑AACGCGCCTTTCGCCCCGCAACGCCAGACCCGTGAc                 <  2:153291/149‑1 (MQ=255)
                                                                                                                                tGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCTGC‑AACCCCACCGCAAAGGAAAACCAACAATG‑AACGCGCCTTTCGCCCCGCAACGCCAGACCCGTGACTAc              >  5:304131/1‑149 (MQ=255)
                                                                                                                                  gCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCTGCAAACCCCACCGCAAAGGAAAACCAACAATG‑AACGCGcc                                             <  1:268561/117‑1 (MQ=255)
                                                                                                                                  gCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCTGC‑AACCCCACCGCAAAGGAAAACCAACAATG‑AACGCGCCTTTCGCCCCGCACCGCCAGACCCGTGACTAc              >  1:195152/1‑147 (MQ=255)
                                                                                                                                    cAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCTGC‑AACCCCACCGCAAAGGAAAACCAACAATG‑AACGCGCCTTTCGCCCCGCAACGCCAGACCCGTGACTACCAgg          >  5:347395/1‑149 (MQ=255)
                                                                                                                                    cAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCTGC‑AACCCCACCGCAAAGGAAAACCAACAATG‑AACGCGCCTTTCGCCCCGCAACGCCAGACCCGTGACTACCAgg          >  4:198063/1‑149 (MQ=255)
                                                                                                                                          ccAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCTGC‑AACCCCACCGCAAAGGAAAACCAACAATG‑AACGCGCCTTTCGCCCCGCAACGCCAGACCCGTGACTACCAGGCAGcc     >  5:27050/1‑148 (MQ=255)
                                                                                                                                             gggCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCTGC‑AACCCCACCGCAAAGGAAAACCAACAATGTAACGCGCCTTTCGCCCCGCAACGCCAGACCCGTGACTACCAGGCAGCCGAt  <  1:101009/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CTCGACCAAGTCGCTGGCCGAGCGGTTGGGAGTGTCGGAGGACACCATCAAGTCGCACCGCAAGAACGTCTACGCCAAGCTCGACATCGGCACCCAGTCCGAACTGTTCTCCCTGTTCATCGATGCGCTGGCCAATGCCCAGGGCGTGCTGGGCAAGGACCCGCTGGAAAGCTACATGGGCAAGCTGCGCTGAACGCCAGCTGCCCTGC‑AACCCCACCGCAAAGGAAAACCAACAATG‑AACGCGCCTTTCGCCCCGCAACGCCAGACCCGTGACTACCAGGCAGCCGAT  >  NC_002947/2955428‑2955716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑