Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,986,195 T→A 8.5% K735M (AAG→ATG)  PP_2612 ← membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,986,1950TA 8.5% 140.2 / 3.7 47K735M (AAG→ATG) PP_2612membrane protein
Reads supporting (aligned to +/- strand):  ref base T (20/23);  new base A (2/2);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CAGCCTGCAAAGGGAATGAGGACTGTCGTGCCTGCGATGGCAAGGCTTCCTTCTAGAACAAGAATCGAACCTAAAGCAGTAGTCGCGCTGGCAGCCGCTGTATATAAGCCTGAAATAAGATTGCCGTTCTGCCATTGACGTATTGCCTTAAAAATATCTGAGGAAAACCCCATTATTATCGCCGGGTAGCCTGATGCCCGCGCGAAAAGATTACTCGTCAGAAAGTTGATGATGCCGTTACCAAAGGCCATGCCTGGCGCCGTTGAGATCCACTTCAACATCATGGCCTTTTGCG  >  NC_002947/2986048‑2986342
                                                                                                                                                   |                                                                                                                                                   
cAGCCTGCAAAGGGAATGAGGACTGTCGTGCCTGCGATGGCAAGGCTTCCTTCTAGAACAAGAATCGAACCTAAAGCAGTAGTCGCGCTGGCAGCCGCTGTATATAAGCCTGAAATAAGATTGCCGTTCTGCCATTGACGTATTGCCt                                                                                                                                                     >  2:355891/1‑148 (MQ=255)
        aaaGGGAATGAGGACTGTCGTGCCTGCGATGGCAAGGCTTCCTTCTAGAACAAGAATCGAACCTAAAGCAGTAGTCGCGCTGGCAGCCGCTGTATATAAGCCTGAAATAAGATTGCCGTTCTGCCATTGACGTATTGCCTTAAAAatat                                                                                                                                            <  3:117076/149‑1 (MQ=255)
          aGGGAATGAGGACTGTCGTGCCTGCTATGGCAAGGCTTCCTTCTAGAACAAGAATCGAACCTAAAGCAGTAGTCGCGCTGGCAGCCGCTGTATATAAGCCTGAAATAAGATTGCCGTTCTGCCATTGACGTATTGCCTTAAAAATATCt                                                                                                                                          <  3:27117/149‑1 (MQ=255)
            ggAATGAGGACTGTCGTGCCTGCGATGGCAAGGCTTCCTTCTAGAACAAGAATCGAACCTAAAGCAGTAGTCGCGCTGGCAGCCGCTGTATATAAGCCTGAAATAAGATTGCCGTTCTGCCATTGACGTATTGCCTTAAAAATATCTGa                                                                                                                                        <  1:300098/149‑1 (MQ=255)
                           gTGCCTGCGATGGCAAGGCTTCCTTCTAGAACAAGAATCGAACCTAAAGCAGTAGTCGCGCTGGCAGCCGCTGTATATAAGCCTGAAATAAGATTGCCGTTCTGCCATTGACGTATTGCCTTAAAAATATCTGAGGAAAACCCCatta                                                                                                                          <  7:217691/148‑1 (MQ=255)
                                 gCGATGGCAAGGCTTCCTTCTAGAACAAGAATCGAACCTAAAGCAGTAGTCGCGCTGGCAGCCGCTGTATATAAGCCTGAAATAAGATTGCCGTTCTGCCATTGACGTATTGCCTTAAAAATATCTGAGGAAAACCCCATTATTATCGc                                                                                                                   >  5:91438/1‑149 (MQ=255)
                                      ggCAAGGCTTCCTTCTAGAACAAGAATCGAACCTAAAGCAGTAGTCGCGCTGGCAGCCGCTGTATATAAGCCTGAAATAAGATTGCCGTTCTGCCATTGACGTATTGCCTTAAAAATATCTGAGGAAAACCCCATTATTATCGCCggg                                                                                                               >  3:268018/1‑148 (MQ=255)
                                       gCAAGGCTTCCTTCTAGAACAAGAATCGAACCTAAAGCAGTAGTCGCGCTGGCAGCCGCTGTATATAAGCCTGAAATAAGATTGGCGTGCTGCCATTGACGTATTGCCTTAAAAATATCTGAGGAAAACCCCATTATTATCGCCggg                                                                                                               >  7:164673/1‑147 (MQ=255)
                                         aaGGCTTCCTTCTAGAACAAGAATCGAACCTAAAGCAGTAGTCGCGCTGGCAGCCGCTGTATATAAGCCTGAAATAAGATTGCCGTTCTGCCATTGACGTATTGCCTTAAAAATATCTGAGGAAAACCCCATTATTATCGCCGGGTAGc                                                                                                           >  5:15140/1‑149 (MQ=255)
                                          aGGCTTCCTTCTAGAACAAGAATCGAACCTAAAGCAGTAGTCGCGCTGGCAGCCGCTGTATATAAGCCTGAAATAAGATTGCCGTTCTGCCATTGACGTATTGCCTTAAAAATATCTGAGGAAAACCCCATTATTATCGCCGGGTAGcc                                                                                                          <  4:268018/149‑1 (MQ=255)
                                                ccttcTAGAACAAGAATCGAACCTAAAGCAGTAGTCGCGCTGGCAGCCGCTGTATATAAGCCTGAAATAAGATTGCCGTTCTGCCATTGACGTATTGCCTTAAAAATATCTGAGGAAAACCCCATTATTATCGCCGGGTAGCCTGAt                                                                                                      <  3:68570/147‑1 (MQ=255)
                                                  ttcTAGAACAAGAATCGAACCTAAAGCAGTAGTCGCGCTGGCAGCCGCTGTATATAAGCCTGAAATAAGATTGCCGTTCTGCCATTGACGTATTGCCTTAAAAATATCTGAGGAAAACCCCATTATTATCGCCGGGTAGCCTGATGccc                                                                                                  >  2:40789/1‑149 (MQ=255)
                                                       gAACAAGAATCGAACCTAAAGCAGTAGTCGCGCTGGCAGCCGCTGTATATAAGCCTGAAATAAGATTGCCGTTCTGCCATTGACGTATTGCCTTAAAAATATCTGAGGAAAACCCCATTATTATCGCCGGGTAGCCTGATGCCcgcgc                                                                                              <  8:15003/148‑1 (MQ=255)
                                                           aaGAATCGAACCTAAAGCAGTAGTCGCGCTGGCAGCCGCTGTATTTAAGCCTGAAATAAGATTGCCGTTCTGGCATTGACGTATTGCCTTAAAAATATCTGAGGAAAACCCCATTATTATCGCCGGGTAGCCTGATGCCCGCGCGaa                                                                                           >  8:62937/1‑147 (MQ=255)
                                                           aaGAATCGAACCTAAAGCAGTAGTCGCGCTGGCAGCCGCTGTATATAAGCCTGAAATAAGATTGCCGTTCTGCCATTGACGTATTGCCTTAAAAATATCTGAGGAAAACCCCATtattat                                                                                                                      <  8:251259/120‑1 (MQ=255)
                                                           aaGAATCGAACCTAAAGCAGTAGTAGCGCTGGCAGCCGCTGTATATAAGCCTGAAATAAGATTGCCGTTCTGCCATTGACGTATTGCCTTAAAAATATCTGAGGAAAACCCCATtattat                                                                                                                      >  7:251259/1‑120 (MQ=255)
                                                             gAATCGAACCTAAAGCAGTAGTCGCGCTGGCAGCCGCTGTATATAAGCCTGAAATAAGATTGCCGTTCTGCCATTGACGTATTGCCTTAAAAATATCTGAGGAAAACCCCATTATTATCGCCGGGTAGCCTGATGCCCGCGCGAAAAGa                                                                                       <  1:355891/149‑1 (MQ=255)
                                                              aaTCGAACCTAAAGCAGTAGTCGCGCTGGCAGCCGCTGTATATAAGCCTGAAATAAGATTGCCGTTCTGCCATTGACGTATTGCCTTaaaa                                                                                                                                                >  4:106856/1‑91 (MQ=255)
                                                              aaTCGAACCTAAAGCAGTAGTCGCGCTGGCAGCCGCTGTATATAAGCCTGAAATAAGATTGCCGTTCTGCCATTGACGTATTGCCTTaaaa                                                                                                                                                <  3:106856/91‑1 (MQ=255)
                                                                       tAAAGCAGTAGTCGCGCTGGCAGCCGCTGTATATAAGCCTGAAATAAGATTGCCGTTCTGCCATTGACGTATTGCCTTAAAAATATCTGAGGAAAACCCCATTATTATCGCCGGGTAGCCTGATGCCCGCGCGAAAAGATTACTCGt                                                                               >  4:267639/1‑147 (MQ=255)
                                                                           gCAGTAGTCGCGCTGGCAGCCGCTGTATATAAGCCTGAAATAAGATTGCCGTTCTGCCATTGACGTATTGCCTTAAAAATATCTGAGGAAAACCCCATTATTATCGCCGGGTAGCCTGATGCCCGCGCGAAAAGATTACTCGTCAGaaa                                                                         <  6:15140/149‑1 (MQ=255)
                                                                           gCAGTAGTCGCGCTGGCAGCCGCTGTATATAAGCCTGAAATAAGATTGCCGTTCTGCCATTGACGTATTGCCTTAAAAATATCTGAGGAAAACCCCATTATTATCGCCGGGTAGCCTGATGCCCGCGCGAAAAGATTACTCGTCAGaaa                                                                         >  4:225158/1‑149 (MQ=255)
                                                                                  tCGCGCTGGCAGCCGCTGTATATAAGCCTGAAATAAGATTGCCGTTCTGCCATTGACGTATTGCCTTAAAAATATCTGAGGAAAACCCCatta                                                                                                                          <  7:237195/93‑1 (MQ=255)
                                                                                  tCGCGCTGGCAGCCGCTGTATATAAGCCTGAAATAAGATTGCCGTTCTGCCATTGACGTATTGCCTTAAAAATATCTGAGGAAAACCCCatta                                                                                                                          >  8:237195/1‑93 (MQ=255)
                                                                                    gcgcTGGCAGCCGCTGTATATAAGCCTGAAATAAGATTGCCGTTCTGCCATTGACGTATTGCCTTAAAAATATCTGAGGAAAACCCCATTATTATCGCCGGGTAGCCTGATGCCCGCGCGAAAAGATTACTCGTCAGAAAGTTgatgat                                                                >  3:209763/1‑149 (MQ=255)
                                                                                              ccGCTGTATATAAGCCTGAAATAAGATTGCCGTTCTGCCATTGACGTATTGACTTAAAAATATCTGAGGAAAACCCCATTATTATCGCCGGGTAGCCTGATGCCCGCGCGAAAAGATTACTCGTCAGAAAGTTGATGATGCCGTTAc                                                        <  3:225158/147‑1 (MQ=255)
                                                                                               cGCTGTATATAAGCCTGAAATAAGATTGCCGTTCTGCCATTGACGTATTGCCTTAAAAATATCTGAGGAAAACCCCATTATTATCGCCGGGTAGCCTGATGCCCGCGCGAAAAGATTa                                                                                    >  7:54746/1‑118 (MQ=255)
                                                                                               cGCTGTATATAAGCCTGAAATAAGATTGCCGTTCTGCCATTGACGTATTGCCTTAAAAATATCTGAGGAAAACCCCATTATTATCGCCGGGTAGCCTGATGCCCGCGCGAAAAGATTa                                                                                    <  8:54746/118‑1 (MQ=255)
                                                                                                gCTGTATATAAGCCTGAAATAAGATTGCCGTTCTGCCATTGACGTATTGCCTTAAAAATATCTGAGGAAAACCCCATTATTATCGCCGGGTAGCCTGATGCCCGCGCGAAAAGATTACTCGTCAGAAAGTTGATGATGCCGTTACCa                                                      >  4:381107/1‑147 (MQ=255)
                                                                                                 cTGTATATAAGCCTGAAATAAGATTGCCGTTCTGCCATTGACGTATTGCCTTAAAAATATCTGAGGAAAACCCCATTATTATCGCCGGGTAGCCTGATGCCCGCGCGAAAAGATTACTCGTCAGAAAGTTGATGATGCCGTTACCAAAg                                                   <  6:198649/149‑1 (MQ=255)
                                                                                                                     aGATTGCCGTTCTGCCATTGACGTATTGCCTTAAAAATATCTGAGGAAAACCCCATTATTATCGCCGGGTAGCCTGATGCCCGCGCGAAAAGATTACTCGTCAGAAAGTTGATGATGCCGTTACCAAAGGCCATGCCTGGCGCCGTTga                               <  1:40789/149‑1 (MQ=255)
                                                                                                                     aGATTGCCGTTCTGCCATTGACGTATTGCCTTAAAAATATCTGAGGAAAACCCCATTATTATCGCCGGGTAGCCTGATGCCCGCGCGAAAAGATTACTCGTCAGAAAGTTGATGATGCCGTTACCAAAGGCCATGCCTGGCGCCGTTga                               <  6:243927/149‑1 (MQ=255)
                                                                                                                        ttGCCGTTCTGCCATTGACGTATTGCCTTAAAAATATCTGAGGAAAACCCCATTATTATCGCCGGGTAGCCTGATGCCCGCGCGAAAAGATTACTCGTCAGAAAGTTGATGATGCCGTTACCAAAGGCCATGCCTGGCGCCGTTgaga                             <  8:280755/148‑1 (MQ=255)
                                                                                                                         tGCCGTTCTGCCATTGACGTATTGCCTTAAAAATATCTGAGGAAAACCCCATTATTATCGCCGGGTAGCCTGATGCCCGCGCGAAAAGATTACTCGTCAGAAAGTTGATGATGCCGTTACCAAAGGCCATGCCTGGCGCCGTTGAGATc                           >  6:330953/1‑149 (MQ=255)
                                                                                                                                   ccATTGACGTATTGCCTTAAAAATATCTGAGGAAAACCCCATTATTATCGCCGGGTAGCCTGATGCCCGCGCGAAAAGATTACTCGTCAGAAAGTTGATGATGCCGTTACCAAAGGCCATGc                                            <  1:215772/122‑1 (MQ=255)
                                                                                                                                   ccATTGACGTATTGCCTTAAAAATATCTGAGGAAAACCCCATTATTATCGCCGGGTAGCCTGATGCCCGCGCGAAAAGATTACTCGTCAGAAAGTTGATGATGCCGTTACCAAAGGCCATGc                                            >  2:215772/1‑122 (MQ=255)
                                                                                                                                    cATTGACGTATTGCCATAAAAATATCTGAGGAAAACCCCATTATTATCGCCGGGTAGCCTGATGCCCGCGCGAAAAGATTACTCGTCAGAAAGTTGATGATGCCGTTACCAAAGGCCATGCCTGGCGCCGTTGAGATCCACTTCAAcat                <  3:188243/149‑1 (MQ=255)
                                                                                                                                    cATTGACGTATTGCCATAAAAATATCTGAGGAAAACCCCATTATTATCGCCGGGTAGCCTGATGCCCGCGCGAAAAGATTACTCGTCAGAAAGTTGATGATGCCGTTACCAAAGGCCATGCCTGGCGCCGTTGAGATCCACTTCAAcat                <  3:194493/149‑1 (MQ=255)
                                                                                                                                    cATTGACGTATTGCCATAAAAATATCTGAGGAAAACCCCATTATTATCGCCGGGTAGCCTGATGCCCGCGCGAAAAGATTACTCGTCAGAAAGTTGATGATGCCGTTACCAAAGGCCATGCCTGGCGCCGTTGAGATCCACTTCAAca                 >  4:194493/1‑148 (MQ=255)
                                                                                                                                    cATTGACGTATTGCCATAAAAATATCTGAGGAAAACCCCATTATTATCGCCGGGTAGCCTGATGCCCGCGCGAAAAGATTACTCGTCAGAAAGTTGATGATGCCGTTACCAAAGGCCATGCCTGGCGCCGTTGAGATCCACTTCAAca                 >  4:188243/1‑148 (MQ=255)
                                                                                                                                     aTTGACGTATTGCCTTAAAAATATCTGAGGAAAACCCCATTATTATCGCCGGGTAGCCTGATGCCCGCGCGAAAAGATTACTCGTCAGAAAGTTGATGATGCCGTTACCAAAGGCCATGCCTGGCGCCGTTGAGATCCACTTCAAcat                >  6:127614/1‑148 (MQ=255)
                                                                                                                                     aTTGACGTATTGCCTTAAAAATATCTGAGGAAAACCCCATTATTATCGCCGGGTAGCCTGATGCCCGCGCGAAAAGATTACTCGTCAGAAAGTTGATGATGCCGTTACCAAAGGCCATGCCTGGCGCCGTTGAGATCCACTTCAAcat                >  2:329308/1‑148 (MQ=255)
                                                                                                                                      ttGACGTATTGCCTTAAAAATATCTGAGGAAAACCCCATTATTATCGCCGGGTAGCCTGAt                                                                                                      >  6:281268/1‑61 (MQ=255)
                                                                                                                                      ttGACGTATTGCCTTAAAAATATCTGAGGAAAACCCCATTATTATCGCCGGGTAGCCTGAt                                                                                                      <  5:281268/61‑1 (MQ=255)
                                                                                                                                         aCGTATTGCCTTAAAAATATCTGAGGAAAACCCCATTATTATCGCCGGGTAGCCTGATGCCCGCGCGAAAAGATTACTCGTCAGAAAGTTGATGATGCCGTTACCAAAGGCCATGCCTGGCGCCGTTGAGATCCACTTCAACATCATg            <  3:381107/148‑1 (MQ=255)
                                                                                                                                                gCCTTAAAAATATCTGAGGAAAACCCCATTATTATCGCCGGGTAGCCTGATGCCCGCGCGAAAAGATTACTCGTCAGAAAGTTGATGATGCCGTTACCAAAGGCCATGCCTGGCGCCGTTGAGATCCACTTCAACATCATGGCCtttt     <  5:330953/148‑1 (MQ=255)
                                                                                                                                                gCCTTAAAAATATCTGAGGAAAACCCCATTATTATCGCCGGGTAGCCTGATGCCCGCGCGAAAAGATTACTCGTCAGAAAGTTGATGATGCCGTTACCAAAGGCCATGCCTGGCGCCGTTGAGATCCACTTCAACATCATGGCCtttt     <  4:209763/148‑1 (MQ=255)
                                                                                                                                                  cTTAAAAATATCTGAGGAAAACCCCATTATTATCGCCGGGTAGCCTGATGCCCGCGCGAAAAGATTACTCGTCAGAAAGTTGATGATGCCGTTACCAAAGGCCATGCCTGGCGCCGTTGCGATCCACTTCAACATCATGGCCTTTTGCg  >  4:217259/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CAGCCTGCAAAGGGAATGAGGACTGTCGTGCCTGCGATGGCAAGGCTTCCTTCTAGAACAAGAATCGAACCTAAAGCAGTAGTCGCGCTGGCAGCCGCTGTATATAAGCCTGAAATAAGATTGCCGTTCTGCCATTGACGTATTGCCTTAAAAATATCTGAGGAAAACCCCATTATTATCGCCGGGTAGCCTGATGCCCGCGCGAAAAGATTACTCGTCAGAAAGTTGATGATGCCGTTACCAAAGGCCATGCCTGGCGCCGTTGAGATCCACTTCAACATCATGGCCTTTTGCG  >  NC_002947/2986048‑2986342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑