| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,991,528 | T→A | 7.7% | intergenic (‑49/+26) | vgrG‑I ← / ← hcpC‑I | type VI secretion system protein/hemolysin‑coregulated protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,991,528 | 0 | T | A | 7.7% | 152.9 / 3.3 | 52 | intergenic (‑49/+26) | vgrG‑I/hcpC‑I | type VI secretion system protein/hemolysin‑coregulated protein |
| Reads supporting (aligned to +/- strand): ref base T (26/22); new base A (2/2); total (28/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01 | |||||||||||
GCGCTGATGCCCTCGCGGAGTTCGAACGACACCACTTCGAATGCAGCTTTGCCAACGAGTGCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCGTGAGCCCACGTGATCTTTCGATACGCCAGCGATACTTCAACCAGTTGGGTGTAGTTTTCATTGTCTTT > NC_002947/2991382‑2991673 | gcgcTGATGCCCTCGCGGAGTTCGAACGACACCACTTCGAATGCAGCTTTGCCAACGAGTGCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGCGGGCATgg > 6:280702/1‑149 (MQ=32) catCGCGGAGTTCGAACGACACCACTTCGAATGCAGCTTTGCCAACGAGTGCCTCAAAGATGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGACCGGGCGGGCATGGCCTGCCAggg > 6:54274/3‑149 (MQ=12) cgGAGTTCGAACGACACCACTTCGAATGCAGCCTTGCCAACGCGCGCCTCAAAGCTGTATCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCAtt < 7:344451/149‑1 (MQ=255) cGAACGACACCACTTCGAATGCAGCTTTGCCAACGAGTGCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAg > 5:95729/1‑149 (MQ=33) cTTCGAATGCGGCTTTGCCAACGAGTGCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGcggcgg > 4:135450/1‑149 (MQ=21) cTTCGAATGCAGCTTTGCCAACGAGTGCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGcggcgg < 4:151123/149‑1 (MQ=34) tGCAGCTTTGCCAACGAGTGCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGt > 1:199397/1‑144 (MQ=33) tGCAGCTTTGCCAACGAGTGCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGt < 2:199397/144‑1 (MQ=33) aCGAGTGCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCagag < 4:28280/149‑1 (MQ=32) cTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGgt > 2:343013/1‑149 (MQ=32) aaaGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGtt > 8:262887/1‑149 (MQ=34) gcgcAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATc < 3:135450/149‑1 (MQ=34) aaaTCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCGTGa < 5:280702/149‑1 (MQ=34) aaaTCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCGTGa < 1:169094/149‑1 (MQ=34) gCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATc < 3:100882/134‑1 (MQ=34) gCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATc > 4:100882/1‑134 (MQ=34) gCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCGTGAGCCCACGTGAt > 6:388339/1‑149 (MQ=34) cATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCGTGAGCCCACGTGATCTTTCg < 5:388339/149‑1 (MQ=34) aTAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCGTGAGCCCACGTGAt > 2:45089/1‑142 (MQ=34) aTAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCGTGAGCCCACGTGAt < 1:45089/142‑1 (MQ=34) aTAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCGTGAGCCCACGTGATCTTTCGa < 2:382222/149‑1 (MQ=34) tAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCg < 7:25072/112‑1 (MQ=32) tAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCg > 8:25072/1‑112 (MQ=32) tAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCGTGAGCCCACGTGATCTTTCGa > 2:24822/1‑148 (MQ=34) aaaaGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCGTGAGCCCACGTGATCTTTCGATa > 4:136973/1‑149 (MQ=34) gATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCGTGAGCCCACGTGATCTTTCGATACGcc < 6:95729/149‑1 (MQ=34) gATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCGTGAGCCCACGTGATCTTTCGATACGc > 4:101639/1‑148 (MQ=34) ttGTCCGCCGTCTGAATCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCGt > 6:91135/1‑118 (MQ=35) ttGTCCGCCGTCTGAATCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCGt < 5:91135/118‑1 (MQ=35) ttGTCCGCCGTCTGAATCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCGTGAGCCCACGTGATCTTTCGa > 2:215378/1‑138 (MQ=34) ttGTCCGCCGTCTGAATCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCGTGAGCCCACGTGATCTTTCGa < 1:215378/138‑1 (MQ=34) cgccgTCTGAATCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCCTTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCGTGAGCCCACGTGATGTTTCGATACGCCAGCGCTACt > 3:43095/1‑148 (MQ=255) cgccgTCTGAATCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCGTGAGCCCACGTGATCTTTCGATACGCCAGCGATACtt > 5:286622/1‑149 (MQ=34) gTCTGAATCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGACGTCAGAGCCTTCGGTGtt < 8:10687/98‑1 (MQ=37) gTCTGAATCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGACGTCAGAGCCTTCGGTGtt > 7:10687/1‑98 (MQ=37) gAATCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCGTGAGCCCACGTGATCTTTCGATACGCCAGCGATACTTCAACCAGt > 5:410203/1‑149 (MQ=35) aaTCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCCGGCGGCGGGTGCGCGCCAGGCGTCAGAGCCTTCGGTGTTGGCTACATCGTGAGCCCACGTGATCTTTCGATACGCCAGCGATACTTCAACCa < 4:256114/146‑1 (MQ=21) aaTCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCGTGAGCCCACGTGATCTTTCGATACGCCAGCGATACTTCAACCAg < 6:286622/147‑1 (MQ=34) tCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCGTGAGCCCACGTGATCTTTCTATACGCCAGCGATACTTCAACCAGTTgg < 1:24822/149‑1 (MQ=37) tCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCGTGAGCCCACGTGATCTTTCGATACGCGAGCGATACTTAAACCAGtttg > 6:289325/1‑147 (MQ=255) aaCACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGANCCTTCGGTGTTGGCTACATCGTGAGCCCACGTGATCTTTCGATACGCCAGCGATACTTCAACCAGTTGGgt > 1:130187/1‑149 (MQ=37) aaCACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCGTGAGCCCACGTGATCTTTCGATACGCCAGCGATACTTCAACCAGTTGGgt > 1:125415/1‑149 (MQ=37) acacAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCGTGAGCCCACGTGATCTTTCGATACGCCAGCGa > 6:339408/1‑130 (MQ=35) acacAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCGTGAGCCCACGTGATCTTTCGATACGCCAGCGa < 5:339408/130‑1 (MQ=35) cacaAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCGTGAGCCCACGTGATCTTTCGATACGCCAGCGATACTTCAACCAGTTGGgtg > 3:352288/1‑148 (MQ=37) gCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCGTGAGCCCACGTGATCTTTCGATACGCCAGCGATACTTCAACCAGTTGGGTGTAGtttt < 4:352288/149‑1 (MQ=37) ccccGGGCAGGCCATGCCCGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCGTGAGCCCACGTGATCTTTCGATACGCCAGCGATACTTCAACCAGtt > 1:8645/2‑137 (MQ=11) ccccGGGCAGGCCATGCCCGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCGTGAGCCCACGTGATCTTTCGATACGCCAGCGATACTTCAACCAGtt < 2:8645/136‑1 (MQ=11) cccGGGCAGGCCATGCCCGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCGTGAGCCCAc > 4:134102/1‑99 (MQ=11) cccGGGCAGGCCATGCCCGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCGTGAGCCCAc < 3:134102/99‑1 (MQ=11) ccGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCGTGAGCCCACGTGATCTTTCGATACGCCAGCGATACTTCAACCAGTTGGGTGTAGTTTTCa < 7:262887/149‑1 (MQ=37) gcATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCGTGAGCCCACGTGATCTTTCGATACGCCAGCGATACTTTAACCAGTTGGGTGTAGTTTTCATTGTCttt > 3:38779/1‑149 (MQ=37) | GCGCTGATGCCCTCGCGGAGTTCGAACGACACCACTTCGAATGCAGCTTTGCCAACGAGTGCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGCGGGCATGGCCTGCCCGGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCGTGAGCCCACGTGATCTTTCGATACGCCAGCGATACTTCAACCAGTTGGGTGTAGTTTTCATTGTCTTT > NC_002947/2991382‑2991673 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |