Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,000,771	C→A	6.6%	V75V (GTG→GTT) ‡	PP_2624 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,000,771	0	C	A	6.6%	195.9 / 3.9	61	V75V (GTG→GTT) ‡	PP_2624	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (25/32);  new base A (2/2);  total (27/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.55e-01

CAGTGCTTCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCGGCAGCTCCACCTCAGC  >  NC_002947/3000627‑3000917                                                                                                                                                 |                                                                                                                                                    cAGTGCTTCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGt                                                                                                                                                >  4:359136/1‑149 (MQ=255)    tGCTTCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCa                                                                                                                                             <  4:212604/149‑1 (MQ=255)               gcgcCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTTGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTg                                                                                                                                  <  2:229649/149‑1 (MQ=255)                 gcCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGc                                                                                                                                <  5:185274/149‑1 (MQ=255)                     gcagcTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGcgtcg                                                                                                                            >  7:212083/1‑149 (MQ=255)                        gcTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTc                                                                                                                         <  4:28827/149‑1 (MQ=255)                            gTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAaca                                                                                                                     >  7:46975/1‑149 (MQ=255)                            gTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAac                                                                                                                      <  8:212083/148‑1 (MQ=255)                                  cTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGt                                                                                                               >  6:404123/1‑149 (MQ=255)                                   tCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCTACGTCCAGGCTCACATTGGCGTCGTTc                                                                                                                         <  1:12483/139‑1 (MQ=255)                                   tCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTc                                                                                                                         >  2:12483/1‑138 (MQ=255)                                       gCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAat                                                                                                          <  8:46975/149‑1 (MQ=255)                                       gCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAat                                                                                                          <  1:240260/149‑1 (MQ=255)                                       gCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAat                                                                                                          <  5:404123/149‑1 (MQ=255)                                           cTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAaca                                                                                                                     >  1:349711/1‑134 (MQ=255)                                           cTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAaca                                                                                                                     <  2:349711/134‑1 (MQ=255)                                                     aTCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGAt                                                                                            <  3:359136/149‑1 (MQ=255)                                                      tccggtccggCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATc                                                                                               >  6:70611/1‑145 (MQ=255)                                                      tccggtccggCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATc                                                                                               <  5:70611/145‑1 (MQ=255)                                                      tccggtccggCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATg                                                                                           >  7:154184/1‑149 (MQ=255)                                                      tccgggccggCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATg                                                                                           <  8:173955/149‑1 (MQ=255)                                                         ggtccggCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATgcgc                                                                                        <  8:129757/149‑1 (MQ=255)                                                                 ccGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCt                                                                                 >  2:369420/1‑148 (MQ=255)                                                                  cGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGa                                                                               >  8:70627/1‑149 (MQ=255)                                                                  cGAAATCGTTGATGGA‑CTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGAc                                                                              >  5:162080/1‑149 (MQ=255)                                                                         gTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCa                                                                                                                        <  6:337894/101‑1 (MQ=255)                                                                         gTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCa                                                                                                                        >  5:337894/1‑101 (MQ=255)                                                                             aTGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCaa                                                                     <  2:65388/148‑1 (MQ=255)                                                                             aTGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCaa                                                                     <  7:70627/148‑1 (MQ=255)                                                                                 gacTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAgcgc                                                                 <  6:162080/146‑1 (MQ=255)                                                                                   cTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACg                                                              >  6:330898/1‑149 (MQ=255)                                                                                   cTGAACTCCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACg                                                              >  7:367599/1‑149 (MQ=255)                                                                                        cTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGt                                                         >  6:405417/1‑149 (MQ=255)                                                                                              gTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATc                                                     >  4:52553/1‑147 (MQ=255)                                                                                                ggACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAgg                                                 <  3:52553/149‑1 (MQ=255)                                                                                                 gACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGc                                                >  4:303497/1‑149 (MQ=255)                                                                                                   cGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGcc                                               >  6:188738/1‑148 (MQ=255)                                                                                                     gCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACATGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGaa                                            <  8:66777/149‑1 (MQ=255)                                                                                                      ccAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATc                                                     >  5:376603/1‑139 (MQ=255)                                                                                                      ccAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATc                                                     <  6:376603/139‑1 (MQ=255)                                                                                                       cAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACAGCGTGTTGaacgac                                                                                                       >  8:376150/1‑84 (MQ=255)                                                                                                          cTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGTCGGTCCTc                                                                       >  7:75879/1‑117 (MQ=255)                                                                                                          cTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGTCGGTCCTc                                                                       >  7:81592/1‑117 (MQ=255)                                                                                                          cTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGTCGGTCCTc                                                                       <  8:75879/117‑1 (MQ=255)                                                                                                          cTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGTCGGTCCTc                                                                       <  8:81592/117‑1 (MQ=255)                                                                                                          cTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGc                                       >  3:189027/1‑149 (MQ=255)                                                                                                             ggTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGccc                                     <  3:303497/148‑1 (MQ=255)                                                                                                             ggTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGcc                                      <  8:154184/147‑1 (MQ=255)                                                                                                             ggTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGcc                                      <  5:188738/147‑1 (MQ=255)                                                                                                                 tCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCccagcac                                <  4:189027/149‑1 (MQ=255)                                                                                                                   cGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCCTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATTACCTGACGCTACTCAAGCGCACCGCAGTAATCCAGGCCGAAGTCGCccagcacc                               >  8:254083/1‑148 (MQ=25)                                                                                                                              cGAGCCTGGACGGTACCGTC‑ACGTCCAGTCGCACTTTGGCGTCGTTCAACAGGCTGTTGAATGTATGTTTATATAAGCTCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCTAAGTCGCCCAGAACCAGCATCTTGTGc                   <  8:367599/149‑1 (MQ=255)                                                                                                                                 gCCTGGACGTGACGGTA‑CCGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATc                         <  2:148156/140‑1 (MQ=21)                                                                                                                                 gCCTGGACGTGACGGTA‑CCGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATc                         >  1:148156/1‑140 (MQ=21)                                                                                                                                   cTGGACGTGACGGTA‑CCGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCGGCAg              >  4:372343/1‑149 (MQ=18)                                                                                                                                   cTGGACGTGACGGTA‑CCGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCGGCAg              <  3:372343/149‑1 (MQ=18)                                                                                                                                   cTGGACGGTACCGTC‑ACGTCCAGGCTCGCATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCgg                 >  8:189409/1‑146 (MQ=255)                                                                                                                                   cTGGACGGTACCGTC‑ACGTCCAGGCTCGCATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCGGCa               <  7:189409/148‑1 (MQ=255)                                                                                                                                         ggTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCGGCAGCTCCAc        <  5:330898/149‑1 (MQ=255)                                                                                                                                         ggTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCAACAGCATCTTGTGCGGCAGCTCCAc        <  7:254083/149‑1 (MQ=255)                                                                                                                                               gTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCGGCAGCTCCACCTCAGc  <  5:405417/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                    CAGTGCTTCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTC‑ACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCGGCAGCTCCACCTCAGC  >  NC_002947/3000627‑3000917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑