Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,001,827 T→C 13.8% L184P (CTG→CCG)  PP_2625 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,001,8270TC13.8% 142.2 / 12.6 58L184P (CTG→CCG) PP_2625hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (27/23);  new base C (3/5);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

GGCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAGCACATGTCGGTGGTA  >  NC_002947/3001681‑3001971
                                                                                                                                                  |                                                                                                                                                
ggCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCCAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGc                                                                                                                                                >  6:357806/1‑149 (MQ=255)
   cATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCg                                                                                                                                             >  1:368283/1‑149 (MQ=255)
          cGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATc                                                                                                                                        >  6:87160/1‑147 (MQ=255)
          cGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGa                                                                                                                                      <  5:87160/149‑1 (MQ=255)
          cGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACACCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGGCGGGCAGGCCCCGCCCGGCATCGa                                                                                                                                      <  6:384965/149‑1 (MQ=255)
           ggCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGGCGGGCAGGCCCCGCCCGGCATCGAg                                                                                                                                     <  6:367729/149‑1 (MQ=39)
            gCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAg                                                                                                                                     >  7:389616/1‑148 (MQ=255)
                cAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCg                                                                                                                                       <  4:148342/142‑1 (MQ=255)
                cAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCg                                                                                                                                       >  3:148342/1‑142 (MQ=255)
                     gATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAgg                                                                                                                            >  2:8837/1‑148 (MQ=255)
                                     aTCGAGGCAAGCAGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCa                                                                                                           >  2:86033/1‑149 (MQ=255)
                                           gCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGc                                                                                                         <  1:203794/145‑1 (MQ=255)
                                           gCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGc                                                                                                         >  2:203794/1‑145 (MQ=255)
                                           gCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTAt                                                                                                     <  8:389616/149‑1 (MQ=255)
                                            cAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATc                                                                                                    <  1:8837/149‑1 (MQ=255)
                                               gCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAg                                                                                                 <  5:357806/149‑1 (MQ=255)
                                               gCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAg                                                                                                 >  8:15132/1‑149 (MQ=255)
                                                     tCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTtgt                                                                                            >  7:146154/1‑148 (MQ=255)
                                                     tCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTtgt                                                                                            >  4:118927/1‑148 (MQ=255)
                                                        aGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTtgt                                                                                            >  8:369039/1‑145 (MQ=255)
                                                        aGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTtgt                                                                                            <  7:369039/145‑1 (MQ=255)
                                                          gTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTgtgagtga                                                                                      >  3:182072/1‑149 (MQ=255)
                                                          gTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGACAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTgtgagtg                                                                                       >  6:90941/1‑148 (MQ=255)
                                                                    gCGGGGTGGGCCGGTCTACCCGTGAGACGCCCGGGCAATTGATGGATAACGGCCAACTGCTCGATGCCGGGCGGGGCCCGCCCGCCATCGAGCATATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGa                                                                             <  2:368283/148‑1 (MQ=255)
                                                                     cGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAgg                                                                           <  4:182072/149‑1 (MQ=255)
                                                                               tGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGAt                                                                                                                                >  1:390326/1‑86 (MQ=255)
                                                                               tGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGAt                                                                                                                                <  2:390326/86‑1 (MQ=255)
                                                                                ggTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCg                                                                                                   >  6:269559/1‑114 (MQ=255)
                                                                                ggTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCg                                                                                                   <  5:269559/114‑1 (MQ=255)
                                                                                       ccGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTc                                                          >  1:354104/1‑148 (MQ=255)
                                                                                                    tGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTc                                                          <  2:145858/135‑1 (MQ=255)
                                                                                                    tGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTc                                                          >  1:145858/1‑135 (MQ=255)
                                                                                                      gCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTgg                                          <  2:354104/149‑1 (MQ=255)
                                                                                                         aGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGccc                                       <  5:90941/149‑1 (MQ=255)
                                                                                                           tGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGccccc                                     >  5:340771/1‑149 (MQ=255)
                                                                                                             aTGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCt                                    <  5:151354/148‑1 (MQ=255)
                                                                                                                  gaACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGAt                                >  8:211920/1‑147 (MQ=255)
                                                                                                                      ggCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGcc                           >  6:23723/1‑148 (MQ=255)
                                                                                                                      ggCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGcc                           <  5:23723/148‑1 (MQ=255)
                                                                                                                       gCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCgg                         <  8:153859/149‑1 (MQ=255)
                                                                                                                        ccAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCggg                        <  3:118927/149‑1 (MQ=255)
                                                                                                                               cTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAg                 >  3:13930/1‑149 (MQ=255)
                                                                                                                                tCGATGGCGGGCAGGCCCCGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAg                 >  6:259643/1‑148 (MQ=255)
                                                                                                                                  gATGGCGGGCAGGCCCCGCCCGGCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTtgt                                                                                            >  6:363663/1‑71 (MQ=21)
                                                                                                                                  gATGGCGGGCAGGCCCCGCCCGGCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTtgt                                                                                            <  5:363663/71‑1 (MQ=21)
                                                                                                                                  gATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAg                 >  6:112035/1‑146 (MQ=255)
                                                                                                                                  gATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAg                 <  5:112035/146‑1 (MQ=255)
                                                                                                                                  gATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAGca               >  5:209523/1‑148 (MQ=255)
                                                                                                                                  gATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAGca               <  3:313852/148‑1 (MQ=255)
                                                                                                                                    tggcgggcaggCCCCGCCCGGCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAGcac              <  7:245446/144‑1 (MQ=255)
                                                                                                                                    tggcgggcaggCCCCGCCCGGCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAGcac              >  8:245446/4‑147 (MQ=255)
                                                                                                                                    tGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAGCACAt            <  7:15132/149‑1 (MQ=255)
                                                                                                                                     gCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTtgt                                                                                            >  4:217576/1‑68 (MQ=255)
                                                                                                                                     gCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTtgt                                                                                            <  3:217576/68‑1 (MQ=255)
                                                                                                                                       cgggcgggGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCgg                         <  3:263547/133‑1 (MQ=255)
                                                                                                                                       cgggcgggGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCgg                         >  4:263547/1‑133 (MQ=255)
                                                                                                                                              ggCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAGCACATGTCggtgg    >  2:35106/1‑147 (MQ=255)
                                                                                                                                              ggCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGAACAGCCGGGCGCGAAGCACATGTCGGTGGTa  <  1:35106/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GGCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAGCACATGTCGGTGGTA  >  NC_002947/3001681‑3001971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑