Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,031,089 C→G 7.7% I132M (ATC→ATG)  mgtA → ATP‑dependent magnesium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,031,0890CG 7.7% 166.9 / 4.1 52I132M (ATC→ATG) mgtAATP‑dependent magnesium transporter
Reads supporting (aligned to +/- strand):  ref base C (27/21);  new base G (2/2);  total (29/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.54e-01

AACCCCACGCCCTGGTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCGGGCAAGCCCCGCGCCT  >  NC_002947/3030941‑3031235
                                                                                                                                                    |                                                                                                                                                  
aaCCCCACGCCCTGGTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGtcatc                                                                                                                                                    <  3:109078/149‑1 (MQ=255)
    ccACGCCCTGGTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGtcatcatca                                                                                                                                                >  2:186199/1‑149 (MQ=255)
      aCGCCCTGGTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCATGGtcatgatgat                                                                                                                                               >  3:154656/1‑148 (MQ=255)
             ggTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCa                                                                                                                                       >  6:19571/1‑149 (MQ=255)
              gTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGAcc                                                                                                                                        <  8:104873/147‑1 (MQ=255)
                  aaCTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGt                                                                                                                                   >  7:73800/1‑148 (MQ=255)
                          aaGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCATGGTCATGATGATGATGACCTTGGTCAggtcgg                                                                                                                           <  4:154656/148‑6 (MQ=38)
                          aaGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGAc                                                                                                                                         <  5:316353/134‑1 (MQ=255)
                          aaGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGAc                                                                                                                                         >  6:316353/1‑134 (MQ=255)
                          aaGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTca                                                                                                                           <  5:232122/148‑1 (MQ=255)
                          aaGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGAAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTcag                                                                                                                          >  3:353475/1‑149 (MQ=255)
                            ggCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTcagca                                                                                                                        <  1:186199/149‑1 (MQ=255)
                                    acaacCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCctgctg                                                                                                                >  3:311864/1‑149 (MQ=255)
                                     caacCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCtgctgc                                                                                                               >  4:47434/1‑149 (MQ=255)
                                         ccGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGAt                                                                                                            >  2:52524/1‑148 (MQ=255)
                                           gTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCt                                                                                                         >  5:380732/1‑149 (MQ=255)
                                            ttCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTg                                                                                                        <  5:19571/149‑1 (MQ=255)
                                               aTCTATGTACTGCTGACCATGGCCTGGATCAGCTTCTTCACCTACTACTGGATGCCGTTCAGCTCAGGCTAAGCAGACGACGCCGACCTGACCAAGTTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTggaa                                                                                                     <  4:395131/149‑3 (MQ=255)
                                                tCTATGTACTGCTGACACTGGCCGGGAACAGCTTCGTCACCGACTACTGGATGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAg                                                                                                    >  5:276571/1‑149 (MQ=255)
                                                  tATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGc                                                                                                                               <  4:83705/120‑1 (MQ=255)
                                                  tATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGc                                                                                                                               >  3:83705/1‑120 (MQ=255)
                                                      tACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTAc                                                                                              <  2:352947/149‑1 (MQ=255)
                                                        ctgctgACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCg                                                                                            >  5:313237/1‑149 (MQ=255)
                                                           ctgACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGc                                                                                           <  3:47434/147‑1 (MQ=255)
                                                           ctgACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTc                                                                                         >  4:91455/1‑149 (MQ=255)
                                                               cccTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGcc                                                                                                                     >  4:19308/1‑117 (MQ=255)
                                                               cccTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGcc                                                                                                                     <  3:19308/117‑1 (MQ=255)
                                                                         gATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCg                                                                           <  4:311864/149‑1 (MQ=255)
                                                                           tCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGAc                                                                         <  6:313237/149‑1 (MQ=255)
                                                                                      aCCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAg                                                               >  4:62936/1‑148 (MQ=255)
                                                                                       ccGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACgcgc                                                                     >  4:194209/1‑141 (MQ=255)
                                                                                       ccGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACgcgc                                                                     <  3:194209/141‑1 (MQ=255)
                                                                                              ctGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAg                                                                                  <  8:65680/121‑1 (MQ=255)
                                                                                              ctGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAg                                                                                  >  7:65680/1‑121 (MQ=255)
                                                                                              ctGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGt                                                        <  1:52524/147‑1 (MQ=255)
                                                                                                         tCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGc                                            >  5:185954/1‑148 (MQ=255)
                                                                                                            gcgcAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACg                                        <  3:322621/149‑1 (MQ=255)
                                                                                                              gcAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGgt                                      >  4:395335/1‑149 (MQ=255)
                                                                                                               cAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGgtg                                     <  3:62936/149‑1 (MQ=255)
                                                                                                                   cGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTgc                                 >  5:192518/1‑149 (MQ=255)
                                                                                                                        cAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGcgccgc                            <  4:353475/149‑1 (MQ=255)
                                                                                                                          gacgacGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGcgccgc                            >  7:113042/1‑147 (MQ=255)
                                                                                                                             gacgCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAg                         >  8:26068/1‑147 (MQ=255)
                                                                                                                                  cGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGCCGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAg                         >  3:218339/1‑142 (MQ=255)
                                                                                                                                   gACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCAGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTAGCGCCGCGAGCAGATCgg                 >  8:290274/1‑149 (MQ=255)
                                                                                                                                    aCCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGc                        >  8:305832/1‑141 (MQ=255)
                                                                                                                                    aCCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGc                        <  7:305832/141‑1 (MQ=255)
                                                                                                                                          ccAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGAATCTGCCAGTAGTACCGCTCGAACAAGGCTGCCCACGCGCTCAAAGCAATGCTGCTCACTACGGCCACGGTGTTGCGCCGCCAGCAGATCGTGCaatca          >  1:362278/1‑146 (MQ=255)
                                                                                                                                           catggTCATGATGATGATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCGGGCAAGccc         >  1:217951/4‑149 (MQ=255)
                                                                                                                                             tggTGATGATGATGATGACCTTGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTg                                                       >  3:266070/2‑101 (MQ=255)
                                                                                                                                             tggTCATGATGATGATGACCTTGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTg                                                       <  4:266070/100‑1 (MQ=38)
                                                                                                                                             aGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACgg                                             <  3:144093/111‑1 (MQ=255)
                                                                                                                                             aGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACgg                                             >  4:144093/1‑111 (MQ=255)
                                                                                                                                              ggTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCGGGCAAGCCCcgc      <  6:192518/149‑1 (MQ=255)
                                                                                                                                               gTCATCATCATCATGACCATGATCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCGGGCAAGCCCcgcg     >  3:187267/1‑149 (MQ=255)
                                                                                                                                                  atcatcatcatGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCGGGCAAGCCCCGCGCCt  >  7:186977/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AACCCCACGCCCTGGTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCGGGCAAGCCCCGCGCCT  >  NC_002947/3030941‑3031235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑