Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,032,940 C→T 7.7% I749I (ATC→ATT)  mgtA → ATP‑dependent magnesium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,032,9400CT 7.7% 147.3 / 2.2 52I749I (ATC→ATT) mgtAATP‑dependent magnesium transporter
Reads supporting (aligned to +/- strand):  ref base C (24/24);  new base T (2/2);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.84e-01

TGGTGCTCGAGGAGGGCGTGCTCAAAGGCCGCGAAACTTTCGGCAACATCATGAAGTACCTGTGCATGACTGCCAGTTCCAACTTCGGCAACGTGTTCTCGGTCCTGGTGGCCAGTGCGTTCATTCCGTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTGCCGTGGGACCGCATGGACAAGGAGTTCCTCAGCAAACCACGCAAATGGGATGCCCGTAACATCGGTCGCTTCATGCTGTGGATAGGCCCTACGTCGTCG  >  NC_002947/3032792‑3033087
                                                                                                                                                    |                                                                                                                                                   
tGGTGCTCGAGGAGGGCGTGCTCAAAGGCCGCGAAACTTTCGGCAACATCATGAAGTACCTGTGCATGACTGCCAGTTCCAACTTCGGCAACGTGTTCTCGGTCCTGGTGGCCAGTGCGTTCATTCCGTTCCTGCCGATGCTGGCGATc                                                                                                                                                     <  1:216979/149‑1 (MQ=255)
                  tGCTCAAAGGCCGCGAAACTTTCGGCAACATCATGAAGTACCTGTGCATGACTGCCAGTTCCAACTTCGGCAACGTGTTCTCGGTCCTGGTGGCAAGTGCGTTCATTCCGTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAAc                                                                                                                                   >  2:380935/1‑149 (MQ=255)
                    cTCAAAGGCCGCGAAACTTTCGGCAACATCATGAAGTACCTGTGCATGACTGCCAGTTCCAACTTCGGCAACGTGTTCTCGGTCCTGGTGGCCAGTGCGTTCATTCCGTTCCTGCCGATGCTGGCGATCCACTTGTtgc                                                                                                                                           >  2:76646/1‑139 (MQ=255)
                    cTCAAAGGCCGCGAAACTTTCGGCAACATCATGAAGTACCTGTGCATGACTGCCAGTTCCAACTTCGGCAACGTGTTCTCGGTCCTGGTGGCCAGTGCGTTCATTCCGTTCCTGCCGATGCTGGCGATCCACTTGTtgc                                                                                                                                           <  1:76646/139‑1 (MQ=255)
                       aaaGGCCGCGAAACTTTCGGCAACATCATGAAGTACCTGTGCATGACTGCCAGTTCCAACTTCGGCAACGTGTTCTCGGTCCTGGTGGCCAGTGCATTCATTCCGTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGa                                                                                                                               <  3:310735/148‑1 (MQ=255)
                              gcgAAACTTTCGGCAACATCATGAAGTACCTGTGCATGACTGCCAGTTCCAACTTCGGCAACGTGTTCTCGGTCCTGGTGGCCAGTGCGTTCATTCCGTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGATg                                                                                                                             >  7:45745/1‑143 (MQ=255)
                              gcgAAACTTTCGGCAACATCATGAAGTACCTGTGCATGACTGCCAGTTCCAACTTCGGCAACGTGTTCTCGGTCCTGGTGGCCAGTGCGTTCATTCCGTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGATg                                                                                                                             <  8:45745/143‑1 (MQ=255)
                                     tttCGGCAACATCATGAAGTACCTGTGCATGACTGCCAGTTCCAACTTCGGCAACGTGTTCTCGGTCCTGGTGGCCAGTGCGTTCATTCCGTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTcc                                                                                                                 >  2:383450/1‑148 (MQ=255)
                                     tttCGGCAACATCATGAAGTACCTGTGCATGACTGCCAGTTCCAACTTCGGCAACGTGTTCTCGGTCCTGGTGGCCAGTGCGTTCATTCCATTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTcc                                                                                                                 >  4:309124/1‑148 (MQ=255)
                                         ggCAACATCATGAAGTACCTGTGCATGACTGCCAGTTCCAACTTCGGCAACGTGTTCTCGGTCCTGGTGGCCAGTGCGTTCATTCCGTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGc                                                                                                             <  1:383450/148‑1 (MQ=255)
                                            aaCATCATGAAGTACCTGTGCATGACTGCCAGTTCCAACTTCGGCAACGTGTTCTCGGTCCTGGTGGCCAGTGCGTTCATTCCGTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTc                                                                                                         >  5:53871/1‑149 (MQ=255)
                                                tcatGAAGTACCTGTGCATGACTGCCAGTTCCAACTTCGGCAACGTGTTCTCGGTCCTGGTGGCCAGTGCGTTCATTCCATTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTg                                                                                                     <  3:309124/149‑1 (MQ=255)
                                                          ccTGTGCATGACTGCCAGTTCCAACTTCGGCAACGTGTTCTCGGTCCTGGTGGCCAGTGCGTTCATTCCGTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTTGCTGCCGTGGGAcc                                                                                           >  2:89104/1‑149 (MQ=255)
                                                          ccTGTGCATGACTGCCAGTTCCAACTTCGGCAACGTGTTCTCGGTCCTGGTGGCCAGTGCGTTCATTCCGTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTGCCGTGGGAcc                                                                                           <  8:25449/149‑1 (MQ=255)
                                                                  tGACTGCCAGTTCCAACTTCGGCAACGTGTTCTCGGTCCTGGTGGCCAGTGCGTTCATTCCGTTCCTGCCGATGCTGGCGATTCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTGCCGTGGGACCGCATGGa                                                                                    >  4:402952/1‑148 (MQ=255)
                                                                  tGACTGCCAGTTCCAACTTCGGCAACGTGTTCTCGGTCCTGGTGGCCAGTGCGTTCATTCCGTTCCTGCCGATGCTGGCGATTCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTGCCGTGGGACCGCATGGa                                                                                    >  4:402950/1‑148 (MQ=255)
                                                                     cTGCCAGTTCCAACTTCGGCAACGTGTTCTCGGTCCTGGTGGCCAGTGCGTTCATTCCGTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTGCCGTGGGACCGCATGGACAAg                                                                                <  6:53871/149‑1 (MQ=255)
                                                                     cTGCCAGTTCCAACTTCGGCAACGTGTTCTCGGTCCTGGTGGCCAGTGCGTTCATTCCGTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTGCCGTGGGACCGCATGGACAAg                                                                                >  5:302513/1‑149 (MQ=255)
                                                                      tGCCAGTTCCAACTTCGGCAACGTGTTCTCGGTCCTGGTGGCCAGTGCGTTCATTCCGTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTGCCGTGGGACCGCATGGACAAg                                                                                >  3:266380/1‑148 (MQ=255)
                                                                           gTTCCAACTTCGGCAACGTGTTCTCGGTCCTGGTGGCCAGTGCGTTCATTCCGTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTGCCGTGGGACCGCATGGACAAGGAGTTc                                                                          <  5:377720/149‑1 (MQ=255)
                                                                               gcccTTCGGCAACGTACTCTCGGGCCTGGTGGCCAGTGCGTTCATTCCGTACCTCCAGCTTCTGGCGATCCACTTTTTGCTGCACAACCTGATGTTTTACTTTTCCCAGCTGTCGCTGCCGTTGGACCGAATGGACAAGGAGTTGCTCa                                                                      <  4:258935/146‑1 (MQ=255)
                                                                                 aCTTCGGCAACGTGTTCTCGGTCCTGGTGGCCAGTGCGTTCATTCCGTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTGCCGTGGGACCGCATGGACAAGGAGTTCCTCAg                                                                     >  7:386986/1‑148 (MQ=255)
                                                                                         aaCGTGTTCTCGGTCCTGGTGGCCAGTGCGTTCATTCCGTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTGCCGTGGGACCGCATGGACAAGGAGTTCCTCAGCAAACCACg                                                            <  7:127462/149‑1 (MQ=255)
                                                                                              gtTCTCGGTCCTGGTGGCCAGTGCGTTCATTCCGTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTGCCGTGGGACCGCATGGACAAg                                                                                >  6:19133/1‑124 (MQ=255)
                                                                                              gtTCTCGGTCCTGGTGGCCAGTGCGTTCATTCCGTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTGCCGTGGGACCGCATGGACAAg                                                                                <  5:19133/124‑1 (MQ=255)
                                                                                              gtTCTCGGTCCTGGTGGCCAGTGCGTTCATTCCGTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTGCCGTGGGACCGCATGGACAAGGAGTTCCTCAGCAAACCACGCAAAt                                                       >  3:148892/1‑149 (MQ=255)
                                                                                               ttCTCGGTCCTGGTGGCCAGTGCGTTCATTCCGTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTGCCGTGGGACCGCATGGACAAGGAGTTCCTCAGCAAACCACGCAAATg                                                      <  8:386986/149‑1 (MQ=255)
                                                                                                 ctcGGTCCTGGTGGCCAGTGCGTTCATTCCGTTCCTGCCGATGCTGGCGATTCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTGCCGTGGGACCGCATGGACAAGGAGTTCCTCAGCAAACCACGCAAATgg                                                     <  3:402952/148‑1 (MQ=255)
                                                                                                 ctcGGTCCTGGTGGCCAGTGCGTTCATTCCGTTCCTGCCGATGCTGGCGATTCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTGCCGTGGGACCGCATGGACAAGGAGTTCCTCAGCAAACCACGCAAATgg                                                     <  3:402950/148‑1 (MQ=255)
                                                                                                  tcGGTCCTGGTGGCCAGTGCGTTCATTCCGTTCCTGCCGATGCTGGCGATCCAc                                                                                                                                                  >  5:382207/1‑54 (MQ=255)
                                                                                                  tcGGTCCTGGTGGCCAGTGCGTTCATTCCGTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTGCCGTGGGACCGCATGGACAAGGAGTTCCTCAGCAAACCACGCAAATGGGa                                                   >  8:200634/1‑149 (MQ=255)
                                                                                                    ggTCCTGGTGGCCAGTGCGTTCATTCCGTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTGCCGTGGGACCGCATGGACAAGGAGTTCCTCAGCAAACCACGCAAATggg                                                    <  6:302513/146‑1 (MQ=255)
                                                                                                     gTCCTGGTGGCCAGTGCGTTCATTCCGTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTGCCGTGGGACCGCATGGACAAGGAGTTCCTCAGCAAACCACGCAAATggaggtc                                                >  5:335011/1‑144 (MQ=255)
                                                                                                     gTCCTGGTGGCCAGTGCGTTCATTCCGTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTGCCGTGGGACCGCATGGACAAGGAGTTCCTCAGCAAACCACGCAAATg                                                      <  6:335011/143‑1 (MQ=255)
                                                                                                       ccTGGTGGCCAGTGCGTTCATTCCGTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTGCCGTGGGACCGCATGGACAAGGAGTTCCTCAGCAAACCACGCAAATGGGATGccc                                              <  4:148892/149‑1 (MQ=255)
                                                                                                              gCCAGTGCGTTCATTCCGTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTTGCTGCCGTGGGACCGCATGGACAAGGAGTTCCTCAGCAAACCACGCAAATGGGATGCCCGTAACAt                                       <  1:89104/149‑1 (MQ=255)
                                                                                                                 aGTGCGTTCATTCCGTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTGCCGTGGGACCGCATGGACAAGGAGTTCCTCAGCAAACCACGCAAATGGGATGCCCGTAACATCgg                                    >  8:159457/1‑149 (MQ=255)
                                                                                                                     cGTTAATTCCTTACCTCCCGATGCTGGCGATCCACTTGATGCTGCAGAACCTGATGTAAGACTTTTCCCAGCTGTCCCTGCCATATGACCGAATGGACAAGGAGTTCCTAAGCAAACCACGCAAATCGGATCCCCGTAACATCGGTCg                                 >  8:263082/1‑148 (MQ=255)
                                                                                                                      gTTCATTCCGTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTGCCGTGGGACCGCATGGACAAGGAGTTCCTCAGCAAACCACGCAAATGGGATGCCCGTAACATCGGTCGCt                               >  8:191807/1‑149 (MQ=255)
                                                                                                                        tCATTCCGTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTGCCGTGGGACCGCATGGACAAGGAGTTCCTCAGCATACCACGCAAATGGGATGCCCGTAACATCGGTCGCtt                              >  1:246003/1‑148 (MQ=255)
                                                                                                                         cATTCCGTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTGCCGTGGGACCGCATGGACAAGGAGTTCCTCAGCAAACCACGCAAATGGGATGCCCGTAACATCGGTCGCTTc                             <  7:159457/148‑1 (MQ=255)
                                                                                                                             ccGTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGa                                                                                                                               >  6:123290/1‑46 (MQ=255)
                                                                                                                             ccGTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGa                                                                                                                               <  5:123290/46‑1 (MQ=255)
                                                                                                                              cGTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTGCCGTGGGACCGCATGGACAAGGAGTTCCTCAGCAAACCACGCAAATGGGATGCCCGTAACATCGGTCGCTTc                             <  6:413811/143‑1 (MQ=255)
                                                                                                                              cGTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTGCCGTGGGACCGCATGGACAAGGAGTTCCTCAGCAAACCACGCAAATGGGATGCCCGTAACATCGGTCGCTTc                             >  5:413811/1‑143 (MQ=255)
                                                                                                                               gTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTGCCGTGGGACCGCATGGACAAGGAGTTCCTCAGCAAACCAc                                                             >  4:210308/1‑110 (MQ=255)
                                                                                                                               gTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTGCCGTGGGACCGCATGGACAAGGAGTTCCTCAGCAAACCAc                                                             <  3:210308/110‑1 (MQ=255)
                                                                                                                                   cTGCCGATGCTGGCGAGCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTGCCGTGGGACCGCATGGACAAGGAGTTCCTCAGCAAACCACGCAAATGGGATGCCCGTAACATCGGTCGCTTCATGCTGTGGa                   <  4:266380/148‑1 (MQ=255)
                                                                                                                                            cTGGCGATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTGCCGTGGGACCGCATGGACAAGGAGTTCCTCAGCAAACCACGCAAATGGGATGCCCGTAACATCGGTGGGTTCATGCTGTGGATTGGCCCtcc         >  2:246614/1‑147 (MQ=255)
                                                                                                                                            cTGGCGATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTGCCGTGGGACCGCATGGACAAGGAGTTCCTCAGCAAACCACGCAAATGGGATGCCCGTAACATCGGTCGCTTCATGCTGTGGATAGGCCCTAc         >  5:143794/1‑149 (MQ=255)
                                                                                                                                                 gATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTGCCGTGGGACCGCATGGACTAGGAGTTCCTCAGCAAACCACGCAAATGGGATGCCCGTAACATCGGTCGCTTCATGCTGTGTATAGGCCCTAcgtctt    <  7:263082/149‑3 (MQ=255)
                                                                                                                                                 gATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTGCCGTGGGACCGCATGGACAAGGAGTTCCTCAGCAAACCACGCAAATGGGATGCCCGTAACATCGGTCGCTTCATGCTGTGGATAGGCCCTAcgtcgt    <  1:380935/149‑1 (MQ=255)
                                                                                                                                                   tCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTGCCGTGGGACCGCATGGAGAAGGAGTTCCTCAGCAAACCACGCAAATGGGATGCCCGTAACATCGGTCGCTTCATGCTGTGGATAGGCCCTACGtcgtcg  <  5:41303/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TGGTGCTCGAGGAGGGCGTGCTCAAAGGCCGCGAAACTTTCGGCAACATCATGAAGTACCTGTGCATGACTGCCAGTTCCAACTTCGGCAACGTGTTCTCGGTCCTGGTGGCCAGTGCGTTCATTCCGTTCCTGCCGATGCTGGCGATCCACTTGTTGCTGCAGAACCTGATGTATGACTTTTCCCAGCTGTCGCTGCCGTGGGACCGCATGGACAAGGAGTTCCTCAGCAAACCACGCAAATGGGATGCCCGTAACATCGGTCGCTTCATGCTGTGGATAGGCCCTACGTCGTCG  >  NC_002947/3032792‑3033087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑