Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,048,605 A→C 12.0% intergenic (+60/‑236) PP_2661 → / → PP_2662 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,048,6050AC12.0% 104.0 / 5.1 41intergenic (+60/‑236)PP_2661/PP_2662hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (17/19);  new base C (2/3);  total (19/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGGATGTGCAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCGGGACCAAATCACCGGGCCGTACCCTCCCGC  >  NC_002947/3048459‑3048742
                                                                                                                                                  |                                                                                                                                         
gtggATGTGCAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGc                                                                                                                                         <  5:407071/149‑1 (MQ=255)
gtggATGTGCAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGc                                                                                                                                         >  2:234601/1‑149 (MQ=255)
        gCAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAg                                                                                                                                 >  8:296372/1‑149 (MQ=255)
           ggCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCGCGAAg                                                                                                                                 <  1:118181/146‑1 (MQ=39)
           ggCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCGCGAAg                                                                                                                                 >  2:118181/1‑146 (MQ=39)
                ggCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGc                                                                                                                           >  3:91956/1‑147 (MQ=255)
                    ggcctggcAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAggcg                                                                                                                     <  4:77925/149‑1 (MQ=255)
                    ggcctggcAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAggcg                                                                                                                     >  4:289355/1‑149 (MQ=255)
                     gcctggcAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAggcgg                                                                                                                    >  2:370266/1‑149 (MQ=255)
                       ctggcAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCGCGAAGGGCTGCAAAGCAGcc                                                                                                                  <  8:326787/149‑1 (MQ=37)
                          gcagcaACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGccc                                                                                                                 <  1:370266/147‑1 (MQ=255)
                            agcaACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCTCGCCCGCGAAg                                                                                                                                 <  1:406126/129‑1 (MQ=255)
                            agcaACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGCGCCCGCGAAg                                                                                                                                 >  2:406126/1‑129 (MQ=255)
                            agcaACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGCCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGt                                                                                                             <  7:296372/149‑1 (MQ=255)
                                       cGTTATGAAATGCGGGCTTGCCAGCGTTGGCATGTTGTGTCTTCCTGAAGCCAGGAGATTCCGCTCAGATTATTTATTTGGGGCTGGTTTGCTGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCg                                                                                                  <  2:170891/149‑1 (MQ=255)
                                       cGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGaa                                                                                                                                  >  8:172681/1‑117 (MQ=255)
                                       cGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGaa                                                                                                                                  <  7:172681/117‑1 (MQ=255)
                                               aaTGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTTAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGAt                                                                                           <  8:69273/148‑1 (MQ=255)
                                               aaTGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATa                                                                                          >  7:128675/1‑149 (MQ=255)
                                                   cccGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCg                                                                                      >  7:161771/1‑149 (MQ=255)
                                                     cgattGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGGCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGcc                                                                                    <  3:271447/146‑1 (MQ=255)
                                                      gCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCt                                                                                   <  1:234601/149‑1 (MQ=255)
                                                                 ttCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTcctgc                                                                        <  4:91956/149‑1 (MQ=255)
                                                                 ttCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTcctgc                                                                        <  3:113361/149‑1 (MQ=255)
                                                                    gCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTcctgcctg                                                                     >  5:74693/1‑149 (MQ=255)
                                                                    gCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTcctgcct                                                                      >  2:265173/1‑148 (MQ=255)
                                                                      aGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAg                                                                   <  3:289355/149‑1 (MQ=255)
                                                                                    tGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGACCTTCTCGCGCGAGCCCGCGACGGGCCGCAAGGCGCCCCCGGTTCATTCAATCGTATTGATACCCGCCTCCAACTCCTCCCTGAGCCATAAGTCTCGaa                                                     >  4:382382/1‑149 (MQ=255)
                                                                                     gAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAAc                                                    >  2:308465/1‑149 (MQ=255)
                                                                                      aaGCCATGAGATTCCGATGAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAAc                                                    <  6:141279/148‑1 (MQ=255)
                                                                                      aaGCCAGGAGATTCCTCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGaa                                                     <  1:296204/147‑1 (MQ=255)
                                                                                       aGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGTAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCa                                                  <  3:278899/149‑1 (MQ=255)
                                                                                         ccAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAg                                                 >  6:107929/1‑148 (MQ=255)
                                                                                                 ttCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGc                                        <  1:265173/149‑1 (MQ=255)
                                                                                                 ttCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGc                                        <  5:107929/149‑1 (MQ=255)
                                                                                                                       gggCGGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCGGGACCAAATCACCg                  <  5:269451/149‑1 (MQ=255)
                                                                                                                                   aGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCGGGACCAAATCACCGGGCCGTACCCTc      >  6:195443/1‑149 (MQ=255)
                                                                                                                                      ccTTGGCGGGCGAGCCCGGGAAGGGCCGCAAGGCGGGCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCGGGACCAAATCACCGGGCCGTACCCTCCCg   <  3:382382/149‑1 (MQ=255)
                                                                                                                                       cTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTa                                   >  5:358640/1‑116 (MQ=255)
                                                                                                                                       cTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCgg                               >  6:368423/1‑120 (MQ=255)
                                                                                                                                       cTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCAGTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAACCAGGTAACGGGACCAAATCACCGGGCCGTACCCTCCCGc  >  4:330927/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                         
GTGGATGTGCAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCGGGACCAAATCACCGGGCCGTACCCTCCCGC  >  NC_002947/3048459‑3048742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑