Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,067,110 G→A 8.1% G50D (GGC→GAC)  qedH‑II → quinoprotein ethanol dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,067,1100GA 8.1% 140.4 / 2.6 50G50D (GGC→GAC) qedH‑IIquinoprotein ethanol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (20/25);  new base A (2/2);  total (23/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CCCGATCCCCACGTCGCCCCTTGTTCGCCGTGAGCCTGGTGCTCAGCGCCATGCTGCTTGCCGGCGCGGCTCACGCCGCTGTCAGCAATGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCAATGTCAATAACGTCAAGGAGCTGCGCCCGGTCTGGGCGTTCTCCTTCGGCGGGGAGAAGCAGCGCGGCCAGCAGGCCCAGCCGCTGATCAAGGACGGGGT  >  NC_002947/3066966‑3067248
                                                                                                                                                |                                                                                                                                          
cccGATCCCCACGTCGCCCCTTGTTCGCCGTGAGCCTGGTGCTCAGCGCCATGCTGCTTGCCGGCGCGGCTCACGCCGCTGTCAGCAATGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTg                                                                                                                                        >  4:146317/1‑149 (MQ=255)
         caattCGCCCCTTGTTCGCCGTGAGCCTGGTGCTCAGCGCCATGCTGCTTGCCGCCGCGGCTCACGCCGCTGTCAGCAATGAAGAAATCCTCAAGGACCCGAAGAACCCGCAGCAGAACGTGACCAATGGCCTGGCCGTGCAGGGCCAg                                                                                                                               >  6:5228/5‑149 (MQ=255)
         cACGTCGCCCCTTGTTCGCCGTGAGCCTGGTGCTCAGCGCCATGCTGCTTGCCGGCGCGGCTCACGCCGCTGTCAGCAATGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAg                                                                                                                               >  3:329543/1‑149 (MQ=255)
            gTCGCCCCTTGTTCGCCGTGAGCCTGGTGCTCAGCGCCATGCTGCTTGCCGGCGCGGCTCACGCCGCTGTCAGCAATGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAgcgc                                                                                                                            <  2:142671/149‑1 (MQ=255)
               gCCCCTTGTTCGCCGTGAGCCTGGTGCTCAGCGCCATGCTGCTTGCCGGCGCGGCTCACGCCGCTGTCAGCAATGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTAc                                                                                                                         <  6:16733/149‑1 (MQ=255)
                ccccTTGTTCGCCGTGAGCCTGGTGCTCAGCGCCATGCTGCTTGCCGGCGCGGCTCACGCCGCTGTCAGCAATGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGTGCTACa                                                                                                                        <  4:107923/149‑1 (MQ=255)
                ccccTTGTTCGCCGTGAGCCTGGTGCTCAGCGCCATGCTGCTTGCCGGCGCGGCTCACGCCGCTGTCAGCAATGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTa                                                                                                                          >  1:164291/1‑147 (MQ=255)
                 cccTTGTTCGCCGTGAGCCTGGTGCTCAGCGCCATGCTGCTTGCCGGCGCGGCTCACGCCGCTGTCAGCAATGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACAg                                                                                                                       >  6:424223/1‑149 (MQ=255)
                              tGAGCCTGGTGCTCAGCGCCATGCTGCTTGCCGGCGCGGCTCACGCCGCTGTCAGCAATGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTg                                                                                                          >  4:191100/1‑149 (MQ=255)
                                   cTGGTGCTCAGCGCCATGCTGCTTGCCGGCGCGGCTCACGCCGCTGTCAGCAATGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCaa                                                                                                     <  2:207143/149‑1 (MQ=255)
                                    tGGTGCTCAGCGCCATGCTGCTTGCCGGCGCGGCTCACGCCGCTGTCAGCAATGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACa                                                                                                                        <  6:124416/129‑1 (MQ=255)
                                    tGGTGCTCAGCGCCATGCTGCTTGCCGGCGCGGCTCACGCCGCTGTCAGCAATGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACa                                                                                                                        >  5:124416/1‑129 (MQ=255)
                                      gTGCTCAGCGCCATGCTGCTTGCCGGCGCGGCTCACGCCGCTGTCAGCAATGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGCTCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCAATGt                                                                                                  <  1:340909/149‑1 (MQ=255)
                                      gTGCTCAGCGCCATGCTGCTTGCCGGCGCGGCTCACGCCGCTGTCAGCAATGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCAAt                                                                                                    <  1:152722/147‑1 (MQ=255)
                                       tGCTCTGCGCCTTGCTGCTTGCCGGGGCGGCTCACGCCGCTGTCAGGAAAGAAGACATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCAATGTc                                                                                                 <  2:188670/149‑1 (MQ=255)
                                          tCAGCGCCATGCTGCTTGCCGGCGCGGCTCACGCCGCTGTCAGCAATGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCAATGTCaa                                                                                               >  2:295455/1‑148 (MQ=255)
                                            aGCGCCATGCTGCTTGCCGGCGCGGCTCACGCCGCTGTCAGCAATGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCAATGTCAATaa                                                                                            >  5:92866/1‑149 (MQ=255)
                                            aGCGCCATGCTGCTTGCCGGCGCGGCTCACGCCGCTGTCAGCAATGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCAATGTCAATaa                                                                                            >  8:239129/1‑149 (MQ=255)
                                                      tgctTGCCGGCGCGGCTCACGCCGCTGTCAGCAATGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCAATGTCAATAACGTCAAGGAg                                                                                  >  2:331710/1‑149 (MQ=255)
                                                        ctTGCCGGCGCGGCTCACGCCGCTGTCAGCAATGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCAATGTCAATAACGTCAAGGAGCt                                                                                <  3:191100/149‑1 (MQ=255)
                                                        ctTGCCGGCGCGGCTCACGCCGCTGTCAGCAATGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGACGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCAATGTCAATAACGTCAAGGAGCt                                                                                >  6:238798/1‑149 (MQ=255)
                                                        ctTGCCGGCGCGGCTCACGCCGCTGTCAGCAATGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGACGTGCAGGGCCAGCCCTACAGCCCGCTGGACCTGCTCAATGTCAAAAACGTCCAGGAGCt                                                                                >  6:238795/1‑149 (MQ=255)
                                                        ctTGCCGGAGCTGCTCACGCCGCTGTCAGCAATGAAGAAATCCTCCAGGACCCGAATAACCCGCATCAGATCGTGACCAATGGCGTGGGCGTGCAGGGCCATCGCTACAGCCCGCTGGACCTTCTCAATGTCAATAACGTCAAGGAg                                                                                  <  5:5228/147‑1 (MQ=255)
                                                               gcgcGGCTCACGCCGCTGTCAGCAATGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCAATGTCAATAACGTCAAGGAGCTGCGCCCg                                                                         >  6:78237/1‑149 (MQ=255)
                                                                     cTCACGCCGCTGTCAGCAATGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCAATGTCAATAACGTCAAGGAGCTGCGCCCGGTCTgg                                                                   <  3:146317/149‑1 (MQ=255)
                                                                      tCACGCCGCTGTCAGCAATGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCAAt                                                                                                    <  6:225034/115‑1 (MQ=255)
                                                                      tCACGCCGCTGTCAGCAATGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCAAt                                                                                                    >  5:225034/1‑115 (MQ=255)
                                                                          gccgcTGTCAGCAATGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCAATGTCAATAACGTCAAGGAGCTGCGCCCGGTCTGGGCGtt                                                              <  5:78237/149‑1 (MQ=255)
                                                                               tGTCAGCAATGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCAATGTCAATAACGTCAAGGAGCTGCGCCCGGTCTGGGCGTTCTCCt                                                         >  7:302935/1‑149 (MQ=255)
                                                                                 tCAGCAATGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCAATGTCAATAACGTCAAGGAGCTGCGCCCGGTCTGGGCGTTCTCCtt                                                        <  1:331710/148‑1 (MQ=255)
                                                                                 tCAGCAATGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCAATGTCAATAACGTCAAGGAGCTGCGCCCGGTCTGGGCGTTCTCCTTc                                                       >  7:170468/1‑149 (MQ=255)
                                                                                 tCAGCAATGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCAATGTCAATAACGTCAAGGAGCTGCGCCCGGTCTGGGCGTTCTCCTTc                                                       <  2:309441/149‑1 (MQ=255)
                                                                                 tCAGCAATGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACAGCCCGCTGGAACTGCTCAATGTCAATAACGTCAAGGAGCTGCGCCCGGTCTGGGCGTTCTCCTTc                                                       >  5:11085/1‑149 (MQ=255)
                                                                                    gCAATGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCAATGTCAATAACGTCAAGGAGCTGCGCCCGGTCTgg                                                                   >  8:106472/1‑134 (MQ=255)
                                                                                    gCAATGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCAATGTCAATAACGTCAAGGAGCTGCGCCCGGTCTgg                                                                   <  7:106472/134‑1 (MQ=255)
                                                                                      aaTGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCAATGTCAATAACGTCAAGGAGCTGCGCCCGGTCTGGGCGTTCTCCTTcggcgg                                                  >  1:8942/1‑149 (MQ=255)
                                                                                       aTGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCAATGTCAATAACGTCAAGGAGCTGCGCCCGGTCTGGGCGTTCTCCTTCGGCggg                                                 <  8:170468/149‑1 (MQ=255)
                                                                                       aTGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCAATGTCAATAACGTCAAGGAGCTGCGCCCGGTCTGGGCGTTCTCCTTCGGCggg                                                 <  4:329543/149‑1 (MQ=255)
                                                                                       aTGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCAATGTCAATAACGTCAAGGAGCTGCGCCCGGTCTGGGCGTTCTCCTTCGGCggg                                                 <  6:92866/149‑1 (MQ=255)
                                                                                        tGAAGAAATCCTCCAGGATCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAgg                                                                                                                                    <  4:56268/65‑1 (MQ=255)
                                                                                         gaagaaATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCAATGTCAATAACGTCAAGGAGCTGCGCCCGGTCTGGGCGTTCTCCTTCGGCggg                                                 >  5:262858/1‑147 (MQ=255)
                                                                                              aaTCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCAATGTCAATAACGTCAAGGAGCTGCGCCCGGTCTGGGCGTTCTCCTTCGGCGGGGAGAagc                                          <  6:11085/149‑1 (MQ=255)
                                                                                                     cAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCAATGTCAATAACGTCAAGGAGCTGCGCCCGGTCTGGGCGTTCTCCTTCGGCGGGGAGAAGCAGCGCgg                                   >  1:365724/1‑149 (MQ=255)
                                                                                                              aagaaCCCGCAGCAGATCGTGACCAATGGCCTGGACGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCAATGTCAATAACGTCAAGGAGCTGCGCCCGGTCTGGGCGTTCTCCTTCGGCGGGGAGAAGCAGCGCGGCCAGCAGGc                          <  5:238798/149‑1 (MQ=255)
                                                                                                              aagaaCCCGCAGCAGATCGTGACCAATGGCCTGGACGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCAATGTCAATAACGTCAAGGAGCTGCGCCCGGTCTGGGCGTTCTCCTTCGGCGGGGAGAAGCAGCGCGGCCAGCAGGc                          <  5:238795/149‑1 (MQ=255)
                                                                                                                    ccGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCAATGTCAATAACGTCAAGGAGCTGCGCCCGGTCTGGGCGTTCTCCTTCGGCGGGGAGAAGCAGCGCGGCCAGCAGGCCCAGcc                    <  5:424223/149‑1 (MQ=255)
                                                                                                                        agcagATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCAATGTCAATAACGTCAAGGAGCTGCGCCCGGTCTGGGCGTTCTCCTTCGGCGGGGAGAAGCAGCGCGGCCAGCAgg                           >  3:294415/1‑138 (MQ=255)
                                                                                                                        agcagATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCAATGTCAATAACGTCAAGGAGCTGCGCCCGGTCTGGGCGTTCTCCTTCGGCGGGGAGAAGCAGCGCGGCCAGCAgg                           <  4:294415/138‑1 (MQ=255)
                                                                                                                           agATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCAATGTCAATAACGTCAAGGAGCTGCGCCCGGTCTGGGCGTTCTCCTTCGGCGGGGAGAAGCAGCGCGGCCAGCAGGCCCAGCCGCTGATc             <  8:302935/149‑1 (MQ=255)
                                                                                                                                      aaTGGCCTGGGCGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCAATGTCAATAACGTCAAGGAGCTGCGCCCGGTCTGGGCGTTCTCCTTCGGCGGGGAGAAGCAGCGCGGCCAGCAGGCCCAGCCGCTGATCAAGGACGGGGt  <  2:8942/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                          
CCCGATCCCCACGTCGCCCCTTGTTCGCCGTGAGCCTGGTGCTCAGCGCCATGCTGCTTGCCGGCGCGGCTCACGCCGCTGTCAGCAATGAAGAAATCCTCCAGGACCCGAAGAACCCGCAGCAGATCGTGACCAATGGCCTGGGCGTGCAGGGCCAGCGCTACAGCCCGCTGGACCTGCTCAATGTCAATAACGTCAAGGAGCTGCGCCCGGTCTGGGCGTTCTCCTTCGGCGGGGAGAAGCAGCGCGGCCAGCAGGCCCAGCCGCTGATCAAGGACGGGGT  >  NC_002947/3066966‑3067248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑