| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,076,092 | A→G | 9.8% | intergenic (‑40/+34) | PP_2685 ← / ← PP_2686 | hypothetical protein/transglutaminase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,076,092 | 0 | A | G | 9.8% | 129.2 / 5.5 | 51 | intergenic (‑40/+34) | PP_2685/PP_2686 | hypothetical protein/transglutaminase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (23/23); new base G (3/2); total (26/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.34e-01 | |||||||||||
GAACAACTTGGTGAACGTGGAGATCTGGTCGATGCCGGCATTAGTGCGTGAGTCGCTGA‑TGAAGACAAGCCCGTCCTCCAGGTGCATGGCGACACAGTAAGTCATGCGGTTTCCCTTGTGTTGCGTGTGGAAGGGTC‑TTGCCGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGCGATGCACGTGA‑ACGCTGGCCTGCATCGATTCGGCGCCGCCGCCGCGGCGTACGCCCCTGACCGGGCAGGCATCAAGGTAGTCCAGGCCCACCGCCAGCTTCA > NC_002947/3075948‑3076235 | gAACAACTTGGTGAACGTGGAGATCTGGTCGATGCCGGCATTAGTTCGTGAGTCGCTGA‑TGAAGACAAGCCCGTCCTCCAGGTGCATGGCGACACAGTAAGTCATGCGGTTTCCCTTGTGTTGCGTGTGGAAGGGTC‑TTGCCGCACTcg > 6:209201/1‑149 (MQ=255) aaCGTGGAGATCTGGTCGATGCCGGCATTAGTGCGTGAGTCGCTGA‑TCAAGACAAGCCCGTCCTCCAGGTGCATGGCGACACAGTAAGTCATGCGGTTTCCCTTGTGTTGCGTGTGGAAGGGTC‑TTGCCGCACTCGCGCGGCAAGACCg < 5:222440/149‑1 (MQ=255) cGTGGAGATCTGGTCGATGCCGGCATTAGTGCGTGAGTCGCTGA‑TGAAGACAAGCCCGTCCTCCAGGTGCATGGCGACACAGTAAGTCATGCGGTTTCCCTTGTGTTGCGTGTGGAAGGGTC‑TTGCCGCACTCGCGCGGCAAGACCGGt < 3:74735/149‑1 (MQ=255) cTGGTCGATGCCGGCATTAGTGCGTGAGTCGCTGAGTGAAGACAAGCCCGTCCTCCAGGTGCATGGCGACACAGTAAGTCATGCGGTTTCCCTTGTGTTGCGTGTGGAAGGGTC‑TTGCCGCa > 1:269006/1‑122 (MQ=255) cTGGTCGATGCCGGCATTAGTGCGTGAGTCGCTGA‑TGAAGCCAAGCCCGTCCTCCAGGTGCATGGCGACACAGTAAGTCATGCGGGTTCCCTTGTGTTGCGTGTGGAAGGGTC‑TTGCCGCACTCGCGCGGCAAGACCGGTCTGAAGCCa < 1:82730/149‑1 (MQ=255) cTGGTCGATGCCGGCATTAGTGCGTGAGTCGCTGA‑TGAAGACAAGCCCGTCCTCCAGGTGCATGGCGACACAGTAAGTCATGCGGTTTCCCTTGTGTTGCGTGTGGAAGGGTC‑TTGCCGCa < 2:269006/121‑1 (MQ=255) ggggggATGGCGGCATTAGTGCGTGAGGCGTTTA‑TTAACACATGCCCGTCCTCTTGTTGCCTGCCCACACTTTAAGGATTGCGTTTGCCGTTGTTTTGCGTGTGGACGGGCC‑TTCCCTCACTCGGGGGCAAAGACCGGTCTGAAGCCa < 2:189806/143‑1 (MQ=255) gTCGATGCCGGCATTAGTGCGTGAGTCGCTGA‑TGAAGACAAGCCCGTCCTCCAGGTGCATGGCGACACAGTAAGTCATGCGGTTTCCCTTGTGTTGCGTGTGGAAGGGTC‑TTGCCGCACTCGCGCGGCAAGACCGGTCTGAAGcc < 5:209201/145‑1 (MQ=255) ccGGCATTAGTGCGTGAGTCGCTTA‑GGAAGACAAGCCCGTCCTCCAGGTGCATGCCGACACAGTAAGTCATGCGGTTTCCCTTGTGTTGCGTGTGGAAGGGTC‑TTGCCGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCAc < 5:392915/148‑1 (MQ=255) cGGCATTAGTGCGTGAGTCGCTGA‑TGAAGACAAGCCCGTCCTCCAGGTGCATGGCGACACAGTAAGTCATGCGGTTTCCCTTGTGTTGCGTGTGGAAGGGTC‑TTGCCGCACTcgcgc > 3:380975/1‑117 (MQ=255) cGGCATTAGTGCGTGAGTCGCTGA‑TGAAGACAAGCCCGTCCTCCAGGTGCATGGCGACACAGTAAGTCATGCGGTTTCCCTTGTGTTGCGTGTGGAAGGGTC‑TTGCCGCACTcgcgc < 4:380975/117‑1 (MQ=255) cGGCATTAGTGCGTGAGTCGCTGA‑TGAAGACAAGCCCGTCCTCCAGGTGCATAGCGACACAGTAAGTCATGCGGTTTCCCTTGTGTTGCGTGTGGAAGGGTC‑TTGCCGCACTCGCGCGGCAAGACCGGTCTGaa > 6:115160/1‑134 (MQ=255) cGGCATTAGTGCGTGAGTCGCTGA‑TGAAGACAAGCCCGTCCTCCAGGTGCATAGCGACACAGTAAGTCATGCGGTTTCCCTTGTGTTGCGTGTGGAAGGGTC‑TTGCCGCACTCGCGCGGCAAGACCGGTCTGaa < 5:115160/134‑1 (MQ=255) gCTGA‑TGAAGACAAGCCCGTCCTCCAGGTGCATGGCGACACAGTAAGTCATGCGGTTTCCCTTGTGTTGCGTGTGGAAGGGTC‑TTGCCGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGCGATGCACGTGA‑ACGCTg > 3:81716/1‑149 (MQ=255) ga‑tgaAGACAAGCCCGTCCTCCAGGTGCATGGCGACACAGTAAGTCATGCGGTTTCCCTTGTGTTGCGTGTGGAAGGGTC‑TTGCCGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGTGATGCACGTGA‑ACGCTGGcc > 5:195486/1‑149 (MQ=255) ga‑tgaAGACAAGCCCGTCCTCCAGGTGCATGGCGACACAGTAAGTCATGCGGTTTCCCTTGTGTTGCGTGTGGAAGGGTC‑TTGCCGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGCGATGCACGt < 5:133414/138‑1 (MQ=255) ga‑tgaAGACAAGCCCGTCCTCCAGGTGCATGGCGACACAGTAAGTCATGCGGTTTCCCTTGTGTTGCGTGTGGAAGGGTC‑TTGCCGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGCGATGCACGt > 6:133414/1‑138 (MQ=255) gaAGACAAGCCCGTCCTCCAGGTGCATGGCGACACAGTAAGTCATGCGGTTTCCCTTGTGTTGCGTGTGGAAGGGTC‑TTGCCGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGCGATGCAc > 2:276195/1‑133 (MQ=255) gaAGACAAGCCCGTCCTCCAGGTGCATGGCGACACAGTAAGTCATGCGGTTTCCCTTGTGTTGCGTGTGGAAGGGTC‑TTGCCGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGCGATGCAc < 1:276195/133‑1 (MQ=255) gACAAGCCCGTCCTCCAGGTGCATGGCGACACAGTAAGTCATGCGGTTTCCCTTGTGTTGCGTGTGGAAGGGTC‑TTGCCGCGCGAGTGCGGCAAg < 4:110879/95‑1 (MQ=39) gACAAGCCCGTCCTCCAGGTGCATGGCGACACAGTAAGTCATGCGGTTTCCCTTGTGTTGCGTGTGGAAGGGTC‑TTGCCGCGCGAGTGCGGCAAg > 3:110879/1‑95 (MQ=39) aCAAGCCCGTCCTCCAGGTGCATGGCGACACAGTAAGTCATGCGGTTTCCCTTGTGTTGCGTGTGGAAGGGTC‑TTGCCGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGCGATGCACGTGA‑ACGCTGGCCTGCATc > 8:243582/1‑148 (MQ=255) aaGCCCGTCCTCCAGGTGCATGGCGACACAGTAAGTCATGCGGTTTCCCTTGTGTTGCGTGTGGAAGGGTC‑TTGCCGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGCGATGCACGTGA‑ACGCTGGCCTGCATCGAt > 4:99174/1‑149 (MQ=255) gCCCGTCCTCCAGGTGCATGGCGACACAGTAAGTCATGCGGTTTCCCTTGTGTTGCGTGTGGAAGGGTC‑TTGCCGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTgg > 8:128024/1‑117 (MQ=255) gCCCGTCCTCCAGGTGCATGGCGACACAGTAAGTCATGCGGTTTCCCTTGTGTTGCGTGTGGAAGGGTC‑TTGCCGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTgg < 7:128024/117‑1 (MQ=255) cGTCCTCCAGGTGGATGGCGACACAGTAAGTCATGCGGTTTCCCTTGTGTTGCGTGTGGATGGGTC‑TTGCCGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGCGATGCTCGTGA‑ACGCTGGCCTGCATCGATTCGgc < 2:90201/149‑1 (MQ=255) ggCGACACAGTAAGTCATGCGGTTTCCCTTGTGTTGCGTGTGGAAGGGTC‑TTGCCGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGCGATGCACGTGA‑ACGCTGGCCTGCATCGATTCGGCGCCgcggcggcggcgc < 7:243582/149‑2 (MQ=255) aaGTCATGCGGTTTCCCTTGTGTTGCGTGTGGAAGGGTCATTCCCGCAGACGCCCGGAAAGACCGGGATAAAGCAAGGATGACACTGCCGATGCACTTGA‑ACGATGGCGTGAAAGGATACGCCGCC‑CCGCCCCCGCCTAccccccatca > 2:121499/1‑145 (MQ=255) tGCGGTTTCCCTTGTGTTGCGTGTGGAAGGGTC‑TTGCCGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGTGATGCACGTGA‑ACGCTGGCCTGCATCGATTCGGCGCCGCCGCCGCGGCGTACGCCCCTGACCGggca < 6:195486/149‑1 (MQ=255) tGCGGTTTCCCTTGTGTTGCGTGTGGAAGGGTC‑TTGCCGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGCGATGCACGTGA‑ACGCTGGCCTGCATCGAATTGGCGCCGCCGCCGCGGCGTACGCCCCTGACCggg > 3:353319/1‑147 (MQ=255) gCGGTTTCCCTTGTGTTGCGTGTGGAAGGGTC‑TTGCCGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGCGATGCACGTGA‑ACGCTGGCCTGCATCGATTCGGCGCCGCCGCCGCGGCGTACGCCCCTGACCGggcag > 8:226435/1‑149 (MQ=255) cGGTTTCCCTTGTGTTGCGTGTGGAAGGGTC‑TTGCCGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGCGATGCACGTGA‑ACGCTGGCCTGCATCGATTCGGCGCCGCCGCCGCGGCGTACGCCCCTGACCGggcagg > 2:125120/1‑149 (MQ=255) ggTTTCCCTTGTGTTGCGTGTGGAAGGGTC‑TTGCCGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGCGATGCACGTGA‑ACGCTGGCCTGCATCGATTCGGCGCCGCCGCCGCGGCGTACGCCCCTGACCGggcaggc < 3:99174/149‑1 (MQ=255) ggTTTCCCTTGTGTTGCGTGTGGAAGGGTC‑TTGCCGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGCGATGCACGTGA‑ACGCTGGCCTGCATCGATTCGGCGCCGCCGCCGCGGCGTACGCCCCTGACCGggcaggc < 4:81716/149‑1 (MQ=255) ccTTGTGTTGCGTGTGGAAGGGTC‑TTGCCGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGCGATGCACGTGA‑ACGCTGGCCTGCATCGATTCGGCGCCGCCGCCGCGCCGTACGCCCCTGACCGGGCAGGCATCAAg > 1:56206/1‑149 (MQ=255) ccTTGTGTTGCGGGTGGAAGGGTC‑TTGCCGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGCGATGCACGTTACACGCTGGCCTGCATCGATTCGGCGCCGCCGCCGCGGCGTACGCCCCTGACCGGGCAGGCATCa < 1:121499/148‑1 (MQ=255) cTTGTGTTGCGTGTGGAAGGGTC‑TTGCCGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGCGATGCACGTGA‑ACGCTGGCCTGCATCGATTCGGCGCCGCCGCCGCGGCGTACGCCCCTGACCGGGCAGGCATCAAgg < 2:325303/149‑1 (MQ=255) cTTGTGTTGCGTGTGGAAGGGTC‑TTGCCGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGCGATGCACGTGA‑ACGCTGGCCTGCATCGATTCGGCGCCGCCGCCGCGGCGTACGCCCCTGACCGGGCAGGCATCAAgg > 1:325303/1‑149 (MQ=255) gggtTGCGGGTGGACGGGTC‑GTGCCGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGCGATGCACGTGA‑ACGCTGGCCTGCATCGATTTGGCGCCGCCGCCGCGGCGTACGCCCCTGACCGGGCAGGCATCAAGGTa < 4:353319/146‑1 (MQ=255) tgtgGAAGGGTC‑TTGCCGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGCGATGCACGTGA‑ACGCTGGCCTGCATCGATTCGGCGCCGCCGCCGCGGCGTACGCCCCTGACCGGGCAGGCATCAAGGTAGTCCAGGcc > 4:147609/1‑149 (MQ=255) tgtgGAAGGGTC‑TTGCCGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGCGATGCACGTGA‑ACGCTGGCCTGCATCGATTAGGCGCCGCCGCCGCGGCGTACGCCCCTGACCGGGCAGGCATCAAGGTAGTCCAGGcc > 3:186669/1‑149 (MQ=255) tgGAAGGGTC‑TTGCCGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGCGATGCACGTGA‑ACGCTGGCCTGCATCGATTCGGCGCCGCCGCCGCGGCGTACGCCCCTGACCGGGCAGGCATCAAGGTAGTCCAGGCCCa > 1:286714/1‑149 (MQ=255) gAAGGGTC‑TTGCCGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGCGATGCACGTGA‑ACGCTGGCCTGCATCGATTCGGCGCCGCCGCCGCGGCGTACGCCCCTGACCGGGCAGGCATCAAGGTAGTCCAGGCCCAcc > 5:347275/1‑149 (MQ=255) gggTC‑TTGCCGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGCGATGCACGTGA‑ACGCTGGCCTGCATCGATTCGGCGCCGCCGCCGCGGCGTACGCCCCTGACCGGGCAGGCATCAAGGTAGTCCAGGCCCACCGc > 3:161472/1‑148 (MQ=255) ggTC‑TTGCCGCGCGAGTGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGCGATGCACGTGA‑ACGCTGGCCTGCATCGATTCGGCGCCGCCGCCGCGGCGTACGCCCCTGACCGGGCAGGCATCAAGGTAGTCCAGGCCCACCGc > 2:95385/1‑147 (MQ=255) tC‑TTGCCGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGCGATGCACGTGA‑ACGCTGGCCTGCATCGATTCGGCGCCGCCGCCGCGGCGTACGCCCCTGACCGGGCAGGCATCAAg < 2:211411/127‑1 (MQ=255) tC‑TTGCCGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGCGATGCACGTGA‑ACGCTGGCCTGCATCGATTCGGCGCCGCCGCCGCGGCGTACGCCCCTGACCGGGCAGGCATCAAg > 1:211411/1‑127 (MQ=255) ttGCCGCGCGAGTGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGCGATGCACGTGA‑ACGCTGGCCTGCATCGATTCGGCGCCGCCGCCGCGGCGTACGCCCCTGACCGGGCAGGCATCAAg > 4:315406/1‑125 (MQ=255) ttGCCGCGCGAGTGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGCGATGCACGTGA‑ACGCTGGCCTGCATCGATTCGGCGCCGCCGCCGCGGCGTACGCCCCTGACCGGGCAGGCATCAAg < 3:315406/125‑1 (MQ=255) ccGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGCGATGCACGTGA‑ACGCTGGCCTGCATCGATTCGGCGCCGCCGCCGCGGCGTACGCCCCTGACCGGGCAGGCATCAAGGTAGTCCAGGCCCACCGCCAGCTTCa < 2:286714/148‑1 (MQ=255) ccGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGCGATGCACGTGA‑ACGCTGGCCTGCATCGATTCGGCGCCGCCGCCGCGGCGTACGCCCCTGACCGGGCAGGCATCAAGGTAGTCCAGGCCCACCGCCAGCTTCa < 7:226435/148‑1 (MQ=255) cGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGCGATGCACGTGA‑ACGCTGGCCTGCATCGATTCGGCGCCGCCGCCGCGGCGTACGCCCCTGACCGGGCAGGCATCAAGGTa < 4:362340/124‑1 (MQ=255) cGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGCGATGCACGTGA‑ACGCTGGCCTGCATCGATTCGGCGCCGCCGCCGCGGCGTACGCCCCTGACCGGGCAGGCATCAAGGTa > 3:362340/1‑124 (MQ=255) | GAACAACTTGGTGAACGTGGAGATCTGGTCGATGCCGGCATTAGTGCGTGAGTCGCTGA‑TGAAGACAAGCCCGTCCTCCAGGTGCATGGCGACACAGTAAGTCATGCGGTTTCCCTTGTGTTGCGTGTGGAAGGGTC‑TTGCCGCACTCGCGCGGCAAGACCGGTCTGAAGCCAGTCTGTCACTGGCGATGCACGTGA‑ACGCTGGCCTGCATCGATTCGGCGCCGCCGCCGCGGCGTACGCCCCTGACCGGGCAGGCATCAAGGTAGTCCAGGCCCACCGCCAGCTTCA > NC_002947/3075948‑3076235 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |