Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,215,265 A→G 9.8% M394V (ATG→GTG) ‡ mexD → multidrug RND transporter MexD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,215,2650AG 9.8% 134.3 / 5.1 51M394V (ATG→GTG) ‡mexDmultidrug RND transporter MexD
Reads supporting (aligned to +/- strand):  ref base A (25/21);  new base G (3/2);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01

CATGGTGCTGGTGTTCCTGGTGATGTTCCTGTTCCTGCAGAACGTGCGCTATACCCTGGTCCCGTCGATCGTGGTGCCGGTGTGCCTGCTCGGGAC‑GCTGATGATCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGACGACGCCATCGTGGTGGTGGAGAACGTCGAGCGGTTGATGGCCG‑AGGAAGGTCTGTCGCCGGTGGAGGCGACCATCAAGGCGATGGGGCAGGTGTCCGGGGCGA  >  NC_002947/3215129‑3215412
                                                                                                                                         |                                                                                                                                                    
cATGGTGCTGGTGTTCCTGGTGATGTTCCTGTTCCTGCAGAACGTGCGCTATACCCTGGTCCCGTCGATCGTGGTGCCGGTGTGCCTGCTCGGGAC‑GCTGATGATCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCg                                                                                                                                          >  5:197703/1‑149 (MQ=255)
 aTGGTGCTGGTGTTCCTGGTGATGTTCCTGTTCCTGCAGAACGTGCGCTATACCCTGGTCCCGTCGATCGTGGTGCCGGTGTGCCTGCTCGGGAC‑GCTGATGATCATGAAGCTGCTCGGCTTCTCGGTGAACATGGTCATCATGTTcac                                                                                                                                         >  5:263492/1‑147 (MQ=255)
 aTGGTGCTGGTGTTCCTGGTGATGTTCCTGTTCCTGCAGAACGTGCGCTATACCCTGGTCCCGTCGATCGTGGTGCCGGTGTGCCTGCTCGGGAC‑GCTGATGATCATGAAGCTGCTCGGCTTCTCGGTGAACATGGTCATCATGTTcac                                                                                                                                         <  6:263492/149‑3 (MQ=255)
   ggTGCTGGTGTTCCTGGTGATGTTCCTGTTCATGCAGAACGTGCGCTATACCCTGGTCCCGTCGATCGTGGTGCCGGTGTGCCTGCTCGGGAC‑GCTGATGATCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCa                                                                                                                                       >  7:192434/1‑149 (MQ=255)
          gtgtTCCTGGTGATGTTCCTGTTCCTGCAGAACGTGCGCTATACCCTGGTCCCGTCGATCGTGGTGCCGGTGTGCCTGCTCGGGAC‑GCTGATGATCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCt                                                                                                                                >  2:239284/1‑149 (MQ=255)
             ttCCTGGTGATGTTCCTGTTCCTGCAGAACGTGCGCTATACCCTGGTCCCGTCGATCGTGGTGCCGGTGTGCCTGCTCGGGAC‑GCTGATGATCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTgg                                                                                                                              <  3:82939/148‑1 (MQ=255)
              tCCTGGTGATGTTCCTGTTCCTGCAGAACGTGCGCTATACCCTGGTCCCGTCGATCGTGGTGCCGGTGTGCCTGCTCGGGAC‑GCTGATGATCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGc                                                                                                                             >  1:125203/1‑148 (MQ=255)
                   ttGATGTTCCTGTTCCTGCAGAACGTGCGCTATACCCTGGTCCCGTAGATCGTGGTGGCGGTGTGCCTGCTCGGGAC‑GCTGATGATCATGAAGCTGCTCGGGTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCg                                                                                                                        <  6:318561/147‑1 (MQ=255)
                                   tgCAGAACGTGCGCTATACCCTGGTCCCGTCGATCGTGGTGCCGGTGTGCCTGCTCGGGAC‑GCTGATGATCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGgacgac                                                                                                       >  5:107078/1‑149 (MQ=255)
                                    gCAGAACGTGCGCTATACCCTGGTCCCGTCGATCGTGGTGCCGGTGTGCCTGCTCGGGACTGCTGATGATCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGgacgac                                                                                                       >  1:116793/1‑149 (MQ=255)
                                     cAGAACGTGCGCTATACCCTGGTCCCGTCGATCGTGGTGCCGGTGTGCCTGCTCGGGAC‑GCTGATGATCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGacgacg                                                                                                      <  2:125203/148‑1 (MQ=255)
                                         aCGTGCGCTATACCCTGGTCCCGTCGATCGTGGTGCCGGTGTGCCTGCTCGGGAC‑GCTGATGATCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGACGACGCCATc                                                                                                 <  1:190383/149‑1 (MQ=255)
                                          cGTGCGCTATACCCTGGTCCCGTCGATCGTGGTGCCGGTGTGCCTGCTCGGGAC‑GCTGATGATCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGACGACGCCATCg                                                                                                >  2:118016/1‑149 (MQ=255)
                                            tGCGCTATACCCTGGTCCCGTCGATCGTGGTGCCGGTGTGCCTGCTCGGGAC‑GCTGATGATCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGGGCTGGCCATCGGCATCCTGGTGGACGACGCCATCgtg                                                                                              >  3:363444/1‑149 (MQ=255)
                                              cgcTATACCCTGGTCCCGTCTATCGTTGTGCCGGTGTGACTGCTCGGGAC‑GCTGATGATCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGACGACGCCATCgtgg                                                                                             <  6:197703/148‑1 (MQ=255)
                                                      ccTGGTCCCGTCGATCGTGGTGCCGGTGTGCCTGCTCGGGAC‑GCTGATGATCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGACGACGCCATCGTGGTGGTGgag                                                                                     >  2:150928/1‑148 (MQ=255)
                                                       cTGGTCCCGTCGATCGTGGTGCCGGTGTGCCTGCTCGGGAC‑GCTGATGATCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGACGACGCCATCGTGGTGGTGGAGaa                                                                                   <  7:265648/149‑1 (MQ=255)
                                                             ccGTCGATCGTGGTGCCGGTGTGCCTGCTCGGGAC‑GCTGATGATCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATc                                                                                                                         >  8:14730/1‑105 (MQ=255)
                                                             ccGTCGATCGTGGTGCCGGTGTGCCTGCTCGGGAC‑GCTGATGATCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCGACATCCTGGTGGACGACGCCATCGTGGTGTTGGAGAACGTCgc                                                                             >  5:321955/1‑148 (MQ=255)
                                                              cGTCGATCGTGGTGCCGGTGTGCCTGCTCGGGAC‑GCTGATGATCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGAcc                                                                                                                                                 <  6:411013/80‑1 (MQ=255)
                                                              cGTCGATCGTGGTGCCGGTGTGCCTGCTCGGGAC‑GCTGATGATCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGAcc                                                                                                                                                 >  5:411013/1‑80 (MQ=255)
                                                              cGTCGATCGTGGTGCCGGTGTGCCTGCTCGGGAC‑GCTGATGATCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATACTGGTGGACGACGCCATCGTGGACGTGGAGAACGTCGAg                                                                            >  8:170164/1‑149 (MQ=255)
                                                               gTCGATCGTGGTGCCGGTGTGCCTGCTCGGGAC‑GCTGATGATCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGACGACGCCATCGTGGTGGTGGAGAACGTCGAGc                                                                           <  3:382602/149‑1 (MQ=255)
                                                               gTCGATCGTGGTGCCGGTGTGCCTGCTCGGGAC‑GCTGATGATCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGACGACGCCATCGTGGTGGTGGAGAACGTCGAGc                                                                           <  1:118016/149‑1 (MQ=255)
                                                                  gATCGTGGTGCCGGTGTGCCTGCTCGGGAC‑GCTGATGATCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGACGACGCCATCGTGGTGGTGGAGAACGTCGAGCgg                                                                         <  1:150928/148‑1 (MQ=255)
                                                                   aTCGTGGTGCCGGTGTGCCTGCTCGGGAC‑GCTGATGATCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGACGACGCCATCGTGGTGGTGga                                                                                      >  6:53511/1‑134 (MQ=255)
                                                                   aTCGTGGTGCCGGTGTGCCTGCTCGGGAC‑GCTGATGATCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGACGACGCCATCGTGGTGGTGga                                                                                      <  5:53511/134‑1 (MQ=255)
                                                                                    ccTGCTCGGGAC‑GCTGATGATCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGACGACGCCATCGTGGTGGTGGAGAACGTCGa                                                                             >  4:301659/1‑126 (MQ=255)
                                                                                    ccTGCTCGGGAC‑GCTGATGATCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGACGACGCCATCGTGGTGGTGGAGAACGTCGa                                                                             <  3:301659/126‑1 (MQ=255)
                                                                                     cTGCTCGGGAC‑GCTGATGATCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGACGACGCCATCGTGGTGGTGGAGAACGTCGAGCGGTTGATGGCCG‑AGGAAGGTCt                                                    >  4:338765/1‑149 (MQ=255)
                                                                                      tGCTCGGGAC‑GCTGATGATCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGACGACGCCATCGTGGTGGTGGAGAACGTCGAGCGGTTGATgg                                                                  <  1:403433/135‑1 (MQ=255)
                                                                                      tGCTCGGGAC‑GCTGATGATCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGACGACGCCATCGTGGTGGTGGAGAACGTCGAGCGGTTGATgg                                                                  >  2:403433/1‑135 (MQ=255)
                                                                                             ggactgtgatgaTCATGAAGCTGCTAGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGACGACGCCATCGTGGTGGTGGAGAACGTCGAGCGGTTGATGGCCG‑AGGAAGGTCTGTCGCCg                                             <  8:192434/143‑1 (MQ=255)
                                                                                                 gCTGATGATCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGACGACGCCATCGTGGTGGTGGAGAACGTCGAGCGGTTGATGGCCG‑Ag                                                            <  5:170113/130‑1 (MQ=255)
                                                                                                 gCTGATGATCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGACGACGCCATCGTGGTGGTGGAGAACGTCGAGCGGTTGATGGCCG‑Ag                                                            >  6:170113/1‑130 (MQ=255)
                                                                                                     atgatCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGACGACGCCATCGTGGTGGTGGAGAACGTCGAGCGGTTGATGGCCG‑AGGAAGGTCTGTCGCCGGTGGAGGc                                     <  2:116793/149‑1 (MQ=255)
                                                                                                       gatCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATCCtg                                                                                                                <  1:104958/73‑1 (MQ=255)
                                                                                                       gatCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATCCtg                                                                                                                >  2:104958/1‑73 (MQ=255)
                                                                                                         tCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGACGACGCCATCGTGGTGGTGGAGAACGTCGAGCGGTTGATGGCCG‑AGGAAGGTCTGTCGCCGGTGGAGGCGAc                                  >  3:15254/1‑148 (MQ=255)
                                                                                                          cATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGACGACGCCATCGTGGTGGTGGAGAACGTCGAGCGGTTGATGGCCG‑AGGAAg                                                        <  8:294162/125‑1 (MQ=255)
                                                                                                          cATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGACGACGCCATCGTGGTGGTGGAGAACGTCGAGCGGTTGATGGCCG‑AGGAAg                                                        >  7:294162/1‑125 (MQ=255)
                                                                                                           aTGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGACGACGCCATCGTGGTGGTGGAGAACGTCGAGCGGTTGATGGCCGCAGGAAGGTCTGTCGCCGGTGGAGGCGAcc                                 <  1:239284/148‑1 (MQ=255)
                                                                                                                gctgctCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGACGACGCCATCGTGGTGGTGGAGAACGTCGAGCGGTTGATGGCCG‑AGGAAGGTCTGTCGGCGGTGGAGGCGACCATCAAgg                          >  2:358047/1‑149 (MQ=255)
                                                                                                                    ctCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGACGACGCCATCGTGGTGGTGGAGAACGTCGAGCGGTTGATGGCCG‑AGGAAGGTCTGTCGCCGGTGGAGGCGACCATCAAGGCGAt                      >  2:81603/1‑149 (MQ=255)
                                                                                                                     tCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGACGACGCCATCGTGGTGGTGGAGAACGTCGAGCGGTTGATGGCCG‑AGGAAGGTCTGTCGCCGGTGGAGGCGACCATCAAGGCGATg                     >  6:81972/1‑149 (MQ=255)
                                                                                                                            ctcGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGACGACGCCATCGTGGTGGTGGAGAACGTCGAGCGGTTGATGGCCG‑AGGAAGGTCTGTCGCCGGTGGAGGCGACCATCAAGGCGATGGGGCAg               <  4:15254/148‑1 (MQ=255)
                                                                                                                            catgccgAACATGGTCATCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGACGACGCCATCGTGGTGGTGGAGAACGTCGAGCGGTTGATGGACG‑AGGAAGGTCTGTCGCCGGTGGAGGCGACCATCAAGGCGATGGGGCAgg              >  5:253526/7‑149 (MQ=25)
                                                                                                                                 cgAACATGGTCATCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGACGACGCCATCGTGGTGGTGGAGAACGTCGAGCGGTTGa                                                                     >  1:379692/2‑90 (MQ=17)
                                                                                                                                 cgAACATGGTCATCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGACGACGCCATCGTGGTGGTGGAGAACGTCGAGCGGTTGa                                                                     <  2:379692/89‑1 (MQ=17)
                                                                                                                                     cATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGACGACGCCATCGTGGTGGTGGAGAACGTCGAGCGGtt                                                                       <  8:31558/84‑1 (MQ=255)
                                                                                                                                     cATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGACGACGCCATCGTGGTGGTGGAGAACGTCGAGCGGtt                                                                       >  7:31558/1‑84 (MQ=255)
                                                                                                                                      atgatgACCATGTTCGGCATGGTGCTGGCCATCGGCATCCAGGTGGACGACGCCATCGTGGTGGTGGAGAACGTCGAGCGGTTGATGGCCG‑AGGAAGGTCTGTCGCCGGTGGAGGCGACCATCAAGGCGATGGGGCAGGTGTCCGGGGc    >  6:342697/1‑149 (MQ=255)
                                                                                                                                        gatgaCCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGACGACGCCATCGTGGTGGTGGAGAACGTCGAGCGGTTGATGGCCG‑AGGAAGGTCTGTCGCCGGTGGAGGCGACCATCAAGGCGATGGGGCAGGTGTCCGGGGCGa  >  2:31803/1‑149 (MQ=255)
                                                                                                                                        gatgaCCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGACGACGCCATCGTGGTGGTGGAGAACGTCGAGCGGTTGATGGCCG‑AGGAAGGTCTGTCGCCGGTGGAGGCGACCATCAAGGCGATGGGGCAGGTGTCCGGGGCGa  >  2:73906/1‑149 (MQ=255)
                                                                                                                                         |                                                                                                                                                    
CATGGTGCTGGTGTTCCTGGTGATGTTCCTGTTCCTGCAGAACGTGCGCTATACCCTGGTCCCGTCGATCGTGGTGCCGGTGTGCCTGCTCGGGAC‑GCTGATGATCATGAAGCTGCTCGGCTTCTCGGTGAACATGATGACCATGTTCGGCATGGTGCTGGCCATCGGCATCCTGGTGGACGACGCCATCGTGGTGGTGGAGAACGTCGAGCGGTTGATGGCCG‑AGGAAGGTCTGTCGCCGGTGGAGGCGACCATCAAGGCGATGGGGCAGGTGTCCGGGGCGA  >  NC_002947/3215129‑3215412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑