Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 402,065 A→C 8.7% I154S (ATC→AGC)  PP_0335 ← glutathione S‑transferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947402,0650AC 8.7% 191.8 / 7.8 67I154S (ATC→AGC) PP_0335glutathione S‑transferase family protein
Reads supporting (aligned to +/- strand):  ref base A (30/31);  new base C (2/4);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGACCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTGGCCGCAGGAAGCTCTCATACCGGCTCGACACTGCCGCATCCATGA  >  NC_002947/401921‑402206
                                                                                                                                                  |                                                                                                                                             
gCGACCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAg                                                                                                                                           >  7:255754/1‑149 (MQ=255)
 cGACCATCGAAGGCCGCTTGAACACCTCCGCATACAACGCCGCCAACCCAGGCTGGCGCTAACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCCCACAGCCC‑‑ACGCCAATCCCCGCCAGGTCGAAGCCGGAGATAAg                                                                                                                                           >  4:56092/1‑148 (MQ=255)
      aTCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCgg                                                                                                                                     >  7:17022/1‑149 (MQ=255)
         gATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGCTGATCTCCGGct                                                                                                                                   <  6:5387/148‑2 (MQ=255)
          aTGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGCTGATCTCCGGcttc                                                                                                                                 <  2:171404/149‑4 (MQ=255)
              ccGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGc                                                                                                                                          >  3:310449/1‑136 (MQ=255)
              ccGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGtgct                                                                                                                             >  6:227922/1‑149 (MQ=255)
                 cTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGtgctcct                                                                                                                          >  7:65640/1‑149 (MQ=255)
                  ttGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGtgctcctg                                                                                                                         >  3:83511/1‑149 (MQ=255)
                     accaccTCGGCATACCATGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGtgctcctgctc                                                                                                                      <  8:172679/149‑1 (MQ=255)
                      ccaccTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGtgctcctgctcc                                                                                                                     <  3:191258/149‑1 (MQ=255)
                      ccaccTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGtgctcctgctcc                                                                                                                     >  4:320923/1‑149 (MQ=255)
                       aaccTCGGCATACCACGCCGCAAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATAAGATCCGGCAAGtgctcctgctc                                                                                                                      >  1:120245/2‑147 (MQ=255)
                        accTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAg                                                                                                                   <  4:121711/149‑1 (MQ=255)
                        accTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAAACGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAg                                                                                                                   <  1:196573/149‑1 (MQ=255)
                         ccTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAgg                                                                                                                  <  2:241562/149‑1 (MQ=255)
                         ccTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAgg                                                                                                                  <  5:117942/149‑1 (MQ=255)
                          cTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGt                                                                                                                 >  2:184057/1‑149 (MQ=255)
                          cTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGt                                                                                                                 >  6:57895/1‑149 (MQ=255)
                               cATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTcc                                                                                                            <  4:83511/149‑1 (MQ=255)
                                   ccACGCCGCCAACCCAGGCTGGGTGTGACGCATGCCAAACCCGGGCTGACGCAAATCGAGATAGCCCAGATCACAGGCC‑‑ACGCCAATCGCCGCCTGTTCGAAGAGGGAGATCAGCTGAGGCAAGTGCTCCTGCTCCAGGTTTTCCAgga                                                                                                        <  2:120245/149‑2 (MQ=255)
                                     aCGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTa                                                                                                      >  3:142543/1‑149 (MQ=255)
                                          gccAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCCGGGCGAAGCCGGAGATCAGCTCCGGGAAGTGCTCCTGCTCCAGGTTGTCCAGGCTAcggcg                                                                                                 >  5:320526/1‑149 (MQ=255)
                                            cAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGa                                                                                               >  1:222837/1‑149 (MQ=255)
                                              aCCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATc                                                                                             <  4:142543/149‑1 (MQ=255)
                                              aCCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATc                                                                                             <  5:227922/149‑1 (MQ=255)
                                               cccAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAgg                                                                                                                  >  7:147583/1‑127 (MQ=255)
                                               cccAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAgg                                                                                                                  <  8:147583/127‑1 (MQ=255)
                                                ccAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCtt                                                                                           >  8:384388/1‑149 (MQ=255)
                                                ccAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCtt                                                                                           <  1:184057/149‑1 (MQ=255)
                                                 cAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTg                                                                                          <  3:56092/149‑1 (MQ=255)
                                                   ggCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTc                                                                                        <  8:17022/149‑1 (MQ=255)
                                                      tGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATc                                                                                             >  2:372955/1‑141 (MQ=255)
                                                      tGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATc                                                                                             <  1:372955/141‑1 (MQ=255)
                                                         cgcTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTg                                                                                  <  5:105366/149‑1 (MQ=255)
                                                          gcTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTgc                                                                                 <  7:335564/149‑1 (MQ=255)
                                                            tCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAg                                                                                                          >  2:53479/1‑122 (MQ=255)
                                                            tCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTgcgc                                                                               >  4:149224/1‑149 (MQ=255)
                                                               cGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTg                                                                            >  6:134451/1‑149 (MQ=255)
                                                                 ccagccaAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGca                                                                          <  5:57895/149‑1 (MQ=255)
                                                                     ccaAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGcagcca                                                                      <  8:255754/149‑1 (MQ=255)
                                                                         aCCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCCCCCTGCGCTTGCAgccagcca                                                                  >  8:344354/1‑149 (MQ=255)
                                                                          cccGGTCTGACGCAAATCCAGATATCCCAGCGCACAGGCCTAACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAg                                                                                                          <  1:333281/110‑1 (MQ=255)
                                                                           ccGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGcagc                                                                        >  5:360215/1‑141 (MQ=255)
                                                                           ccGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGcagc                                                                        <  6:360215/141‑1 (MQ=255)
                                                                             gggCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATcc                                                               >  2:24401/1‑148 (MQ=255)
                                                                                    cGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCtt                                                       <  7:384388/149‑1 (MQ=255)
                                                                                     gCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTg                                                      >  8:145274/1‑149 (MQ=255)
                                                                                            cAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGCTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCctct                                                <  5:134451/148‑1 (MQ=255)
                                                                                              gATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTGGc                                             <  8:65640/149‑1 (MQ=255)
                                                                                                   cccAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTGGCCGCAg                                        <  2:222837/149‑1 (MQ=255)
                                                                                                          cacaGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTGGCCGCAGGAAGctc                                 >  2:205377/1‑149 (MQ=255)
                                                                                                          cacaGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTGGCCGCAGGAAGctc                                 <  8:280055/149‑1 (MQ=255)
                                                                                                          cacaGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTGGCCGCAGGAAGctc                                 <  7:145274/149‑1 (MQ=255)
                                                                                                               gCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTGGCCGCAGGAAGctc                                 <  6:336323/144‑1 (MQ=255)
                                                                                                               gCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTGGCCGCAGGAAGctc                                 >  5:336323/1‑144 (MQ=255)
                                                                                                                    aCGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTGGCCGCAGGAAGCTCTCATAc                           >  3:293394/1‑147 (MQ=255)
                                                                                                                    aCGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTGGCCGCAGGAAGCTCTCATAc                           <  4:293394/147‑1 (MQ=255)
                                                                                                                                        gAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTGGCCGCAGGAAGCTCTCATACCGGCTCGACACTGCCGCATCCa     >  7:87456/1‑149 (MQ=255)
                                                                                                                                        gAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTGGCCGCAGGAAGCTCTCATACCGGCTCGACACTGCCGCATCCa     >  1:75273/1‑149 (MQ=255)
                                                                                                                                         ttgCCGGAGCTGATCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTGGCCGCAGGAAGCTCTCATACCGGCTCGACACTGCCGCATCCAt    >  7:165926/3‑149 (MQ=255)
                                                                                                                                           gCCGGAGCTGATCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTGGCCGCAGGAAGctc                                 <  3:39631/118‑1 (MQ=255)
                                                                                                                                           gCCGGAGCTGATCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTGGCCGCAGGAAGcta                                 >  4:39631/1‑117 (MQ=255)
                                                                                                                                           gCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTGGCCGCAGGAAGCTCTCATACCGGCTCGACACTGCCGCATCCATGa  <  1:24401/149‑1 (MQ=255)
                                                                                                                                            ccGGAGCTGATCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTGGCCGCAGGAAGCTCTCATACCGGCTCGACACTGCCGCATCCATGa  <  8:165926/148‑1 (MQ=255)
                                                                                                                                            ccGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTGGCCGCAGGAAGCTCTCATACCGGCTCGacac                >  8:1482/1‑134 (MQ=255)
                                                                                                                                            ccGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTGGCCGCAGGAAGCTCTCATACCGGCTCGacac                <  7:1482/134‑1 (MQ=255)
                                                                                                                                               gagaTCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTAcggc                                                                                                  <  3:407181/49‑1 (MQ=255)
                                                                                                                                               gagaTCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTAcggc                                                                                                  >  4:407181/1‑49 (MQ=255)
                                                                                                                                                  |                                                                                                                                             
GCGACCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCC‑‑ACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTCCAGGCTACGGCGGATCTTGTCACCCTGCGCTTGCAGCCAGCCATCCCAGCGCTTGTCCTCTGGCCGCAGGAAGCTCTCATACCGGCTCGACACTGCCGCATCCATGA  >  NC_002947/401921‑402206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑