| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,397,589 | T→G | 6.5% | F24V (TTC→GTC) ‡ | PP_3002 → | shikimate dehydrogenase‑like protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,397,589 | 0 | T | G | 6.5% | 189.7 / 4.8 | 62 | F24V (TTC→GTC) ‡ | PP_3002 | shikimate dehydrogenase‑like protein |
| Reads supporting (aligned to +/- strand): ref base T (29/29); new base G (2/2); total (31/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.47e-01 | |||||||||||
GCCGGGCTTGGCTTCAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAGCGAAGGCGGGAAGGGCATGAACATCACCGCCCCGTTCAAGCTGCGTGCCTTCGAGC > NC_002947/3397443‑3397733 | gccgGGCTTGGCTTCAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTc > 2:108565/1‑149 (MQ=255) gggCTTGGCTTCAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGcc < 8:117103/149‑1 (MQ=255) cttggcttCAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAg > 3:2335/1‑149 (MQ=255) cttggcttCAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAg < 7:125656/149‑1 (MQ=255) gcttCAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCa < 2:403282/149‑1 (MQ=255) cttCAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGGGCGAACAGGCCat < 6:223848/149‑2 (MQ=255) cAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGGGCGAACAGGCCatga < 3:382537/148‑4 (MQ=255) aGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAAc < 4:2335/149‑1 (MQ=255) gCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAAcc < 6:1401/149‑1 (MQ=255) ccTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACcag > 3:317007/1‑149 (MQ=255) cTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTAGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCACCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACcag > 7:116725/1‑148 (MQ=255) gaaATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTg > 2:301162/1‑149 (MQ=255) ccTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACgg < 6:402450/147‑1 (MQ=255) ccTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACgg < 6:359092/147‑1 (MQ=255) ttGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCGGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGc > 2:198823/1‑148 (MQ=255) cGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCGGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCt < 1:198823/149‑1 (MQ=255) gtgtTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCACTg > 8:351932/1‑149 (MQ=255) tgttgtTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGAGATCGAAGGCTCGCTg > 7:304923/1‑148 (MQ=255) tttACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGgacga < 6:400071/148‑1 (MQ=255) cACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGaa > 4:100533/1‑128 (MQ=255) gagaTGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGccc < 1:108565/149‑1 (MQ=255) gCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTc > 3:93840/1‑137 (MQ=255) gCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTc < 4:93840/137‑1 (MQ=255) gCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTa > 8:167349/1‑149 (MQ=255) aTGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCGAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCa < 8:116725/149‑1 (MQ=255) gCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACgg < 5:141917/100‑1 (MQ=255) gCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACgg > 6:141917/1‑100 (MQ=255) cGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCa > 3:221633/1‑135 (MQ=255) cGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCa < 4:221633/135‑1 (MQ=255) cGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAg > 1:80956/1‑149 (MQ=255) aGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTc > 1:33554/1‑115 (MQ=255) aGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTc < 2:33554/115‑1 (MQ=255) aGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAGCGAAGGCg > 2:20502/1‑149 (MQ=255) gTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCACCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAGCGAAGGCgg < 1:130069/149‑1 (MQ=255) aTCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAGCGAAGGCcgg > 8:108180/1‑147 (MQ=255) tCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAgg < 7:145833/120‑1 (MQ=255) tCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAgg > 8:145833/1‑120 (MQ=255) ggCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCACTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTACCGAAGGCGGGAAgg < 7:351932/148‑1 (MQ=255) gCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTg > 5:39195/1‑122 (MQ=255) gCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTg < 6:39195/122‑1 (MQ=255) gCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAGCGAAGGCGGGAAGGGc < 2:80956/149‑1 (MQ=255) gCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCATGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTTGTAGCGAAGGCGGGAAGGGc < 3:100099/149‑1 (MQ=255) cgaccgaTCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAGCGAAGGCGGGAAGGGCAt < 7:167349/149‑1 (MQ=255) gaccgaTCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAGCGAAGGCGGGAAGGGCATg < 4:317007/149‑1 (MQ=255) accgaTCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGTCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAg < 7:121405/129‑1 (MQ=255) accgaTCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTAGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAg > 8:121405/1‑129 (MQ=255) gaTCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGAAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGAAGTTTCGTAGCGAAGGCGGGAAGGGCATGAAc > 5:70350/1‑148 (MQ=255) aTCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAg > 3:248919/1‑108 (MQ=255) aTCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAg < 4:248919/108‑1 (MQ=255) ccGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAGCGAAGGCGGGAAGGGCATGAACATCACCGCCCCGTTCAAGCt > 6:114021/1‑149 (MQ=255) cGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAGCGAAGGCGGGAAGGGCATGAACATCACCGCCCCGTTCAAGCTg > 7:202463/1‑149 (MQ=255) gCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAGCGAAGGCGGGAAGGGCATGAACATCACCGCCCCGTTCAAGCTGc < 7:108180/149‑1 (MQ=255) gCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAGCGAAGGCGGGAAGGGCATGAACATCACCGCCCCGTTCAAGCTGc > 1:259871/1‑149 (MQ=255) aTGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATTGAAGGCTCGCTGGACGACTTCGAAGCCCATGTACTGCAGTTTCGTAGCGAAGGCGGGAAGGGCATGAACATCACCGCCCCGTTCAAGCTGc < 3:256250/141‑1 (MQ=255) aTGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATTGAAGGCTCGCTGGACGACTTCGAAGCCCATGTACTGCAGTTTCGTAGCGAAGGCGGGAAGGGCATGAACATCACCGCCCCGTTCAAGCTGc > 4:256250/1‑141 (MQ=255) tGGCCTGGGCGAACAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAGCGAAGGCGGGAAGGGCATGAACATCACCGCCCCGTTCAAGCTGCGTGCCTTc > 3:179281/1‑148 (MQ=255) tGGCCTGGGCGAACAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAGCGAAGGCGGGAAGGGCATGAACATCACCGCCCCGTTCAAGCTGCGTGCCTTCg > 1:10840/1‑149 (MQ=255) ccTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAGCGAAGGCGGGAAGGGCATGAACATCACCGCCCCGTTCAAGCTGc < 1:73380/137‑1 (MQ=255) ccTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAGCGAAGGCGGGAAGGGCATGAACATCACCGCCCCGTTCAAGCTGc > 2:73380/1‑137 (MQ=255) ccTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAGCGAAGGCGGGAAGGGCATGAACATCACCGCCCCGTTCAAGCTGCGTGCCTTCGAGc > 2:265158/1‑149 (MQ=255) gTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAGCGAAGGCGGGAAGGGCAt > 4:327663/1‑108 (MQ=255) gTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAGCGAAGGCGGGAAGGGCAt < 3:327663/108‑1 (MQ=255) | GCCGGGCTTGGCTTCAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAGCGAAGGCGGGAAGGGCATGAACATCACCGCCCCGTTCAAGCTGCGTGCCTTCGAGC > NC_002947/3397443‑3397733 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |