Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,498,264 C→G 7.4% intergenic (‑668/+38) PP_5562 ← / ← PP_5563 hypothetical protein/membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,498,2640CG 7.4% 157.0 / 2.6 51intergenic (‑668/+38)PP_5562/PP_5563hypothetical protein/membrane protein
Reads supporting (aligned to +/- strand):  ref base C (23/24);  new base G (2/2);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATTCGCGGGCAAGCCCGCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGCACACCCGTTCATCGTTGACC  >  NC_002947/3498124‑3498410
                                                                                                                                            |                                                                                                                                                  
cATTCGCGGGCAAGCCCGCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCcgc                                                                                                                                            >  3:6243/1‑149 (MQ=255)
 aTTCGCGGGCAAGCCCGCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGc                                                                                                                                                    >  3:194402/1‑140 (MQ=255)
 aTTCGCGGGCAAGCCCGCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGc                                                                                                                                                    <  4:194402/140‑1 (MQ=255)
     gcgGGCAAGCCCGCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGGCTGCCCGCGaat                                                                                                                                        <  3:367992/148‑2 (MQ=255)
     gcgGGCAAGCCCGCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGGCTGCCCGCGaat                                                                                                                                        >  4:367992/1‑147 (MQ=255)
              cccGCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAg                                                                                                                              >  1:215131/1‑149 (MQ=255)
               ccGCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGc                                                                                                                             >  1:310258/1‑149 (MQ=255)
                 gCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCg                                                                                                                           >  6:268111/1‑149 (MQ=255)
                     cttcATAGACCGGTGGTGTCGCAACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGTCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTACCCGCGAACACCGGCTGCGCCGGtgt                                                                                                                       <  8:159956/146‑3 (MQ=21)
                     cTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACATGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTACCCGCGAACACAGGCTGCGCCGGtgt                                                                                                                       >  7:159956/1‑147 (MQ=25)
                             aCCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCaa                                                                                                                >  2:85189/1‑148 (MQ=255)
                                  tggtgTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAAc                                                                                                          >  4:92051/1‑149 (MQ=255)
                                  tggtgTCGCCACGTTGCAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAAc                                                                                                          >  2:344902/1‑149 (MQ=255)
                                  tcttgtCGCCAGGTGGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGCTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAAc                                                                                                          <  7:14586/146‑1 (MQ=255)
                                    gtgtCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACg                                                                                                         <  2:310258/148‑1 (MQ=255)
                                         gCCACGTTGTAGGAGCTGGTTTACCCGCTAATAGGCCGCACCTCCCGGCCACAATCCCTGGTCATTCACCGTCCCAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCAt                                                                                                    <  6:48203/148‑1 (MQ=37)
                                         gCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATc                                                                                                   >  7:230415/1‑149 (MQ=255)
                                         gCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATc                                                                                                   >  7:38432/1‑149 (MQ=255)
                                          ccACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAAc                                                                                                               <  8:29392/136‑1 (MQ=255)
                                          ccACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATc                                                                                                   >  3:127373/1‑148 (MQ=255)
                                            aCGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGAc                                                                                                <  8:38432/149‑1 (MQ=255)
                                                           gTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGt                                                                                  <  3:92051/148‑1 (MQ=255)
                                                           gTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGt                                                                                  >  1:380597/1‑148 (MQ=255)
                                                             ttACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGtctc                                                                               >  4:136937/1‑149 (MQ=255)
                                                                 ccGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCg                                                                                                  <  1:196941/126‑1 (MQ=255)
                                                                 ccGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTAATCCAACTCAACGACCaaag                                                                                                  >  2:196941/1‑123 (MQ=255)
                                                                           gCCGGACCTGCCGGCCACTATCCCTCGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGcc                                                                 <  1:259010/149‑1 (MQ=255)
                                                                                  cTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATg                                                          <  3:136937/149‑1 (MQ=255)
                                                                                       gtcgaatATCCTTTGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCaa                                                     <  8:112294/143‑1 (MQ=255)
                                                                                          cACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTg                                                  >  1:241451/1‑149 (MQ=255)
                                                                                            cTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTc                                                >  7:206495/1‑149 (MQ=255)
                                                                                               tCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGt                                              <  2:241451/148‑1 (MQ=255)
                                                                                                 ccTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCTAACACGGGCGCAGCCGGTTTCATCCAAGTCAAGGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTg                                            <  1:85189/148‑1 (MQ=255)
                                                                                                  cTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGAAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGAt                                          <  1:344902/149‑1 (MQ=255)
                                                                                                    ggTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGAACTGTACGCACACGTCTCTGCGATCGATTGCCTTGCATGGCCATTTGTCGTTGATg                                         >  8:281995/1‑148 (MQ=255)
                                                                                                     gTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGt                                        <  7:185193/148‑1 (MQ=255)
                                                                                                      tCATTCACCGTCATAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTaa                                      <  4:164087/149‑1 (MQ=255)
                                                                                                        aTTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTg                                                  >  2:314908/1‑135 (MQ=255)
                                                                                                        aTTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTg                                                  <  1:314908/135‑1 (MQ=255)
                                                                                                           cACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATc                                 >  2:192851/1‑149 (MQ=255)
                                                                                                            aCCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATc                                 <  2:215131/148‑1 (MQ=255)
                                                                                                              cGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCgg                              >  1:223429/1‑149 (MQ=255)
                                                                                                                   caGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTg                          >  5:125881/1‑148 (MQ=255)
                                                                                                                    aGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTg                          >  5:173677/1‑147 (MQ=255)
                                                                                                                          cACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGCacac                  <  4:127373/149‑1 (MQ=255)
                                                                                                                            ccGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGCACAccc                >  3:89078/1‑149 (MQ=255)
                                                                                                                              gCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGCACACCCGt              <  1:192851/149‑1 (MQ=255)
                                                                                                                                aTTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGCACACCCGTTc            <  4:406637/149‑1 (MQ=255)
                                                                                                                                   cgcgGGCAGGCTGCCCGAGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGCACACCCGTTCATc         >  3:324510/1‑149 (MQ=255)
                                                                                                                                     cgGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGCACACCCGTTCATCGt       <  6:173677/149‑1 (MQ=255)
                                                                                                                                     cgGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGCACACCCGTTCATCGt       <  6:125881/149‑1 (MQ=255)
                                                                                                                                       ggCAGGCTGCCCGCGAATACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGCACACCCGTTCATCGTTg     <  6:35501/149‑1 (MQ=255)
                                                                                                                                          aGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGCACACCCGTTCATCGTTGAcc  <  2:380597/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                  
CATTCGCGGGCAAGCCCGCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGCACACCCGTTCATCGTTGACC  >  NC_002947/3498124‑3498410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑