Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,510,692	A→G	7.1%	A151A (GCA→GCG)	PP_5575 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,510,692	0	A	G	7.1%	158.7 / 2.7	56	A151A (GCA→GCG)	PP_5575	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (25/27);  new base G (2/2);  total (27/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.73e-01

CGCAGTCGAGGCTTTGCGCGAATTGAACCCGCAACTGCGCGTGATTCGCCAACGTGACTGCCTCCTGGCTGGGAACCCGGCCAAGGTCGTGGACTTTCACTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGACCCCAGCAAGCCCGGCCTGAGCCAGTGGCTGGCCGAGTTCGACCGCATGCTCGATGGCTTCGCGCAGGGCTGACCCCGATTTTTTCGCT  >  NC_002947/3510548‑3510840                                                                                                                                                 |                                                                                                                                                     cgcAGTCGAGGCTTTGCGCGAATTGAACCCGCAACTGCGCGTGATTCGCCAACGTGACTGCCTCCTGGCTGGGAACCCGGCCAAGGTCGTGGACTTTCACTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTgg                                                                                                                                                  <  3:15112/149‑1 (MQ=255)      tCGAGGCTTTGCGCGAATTGAACCCGCAACTGCGCGTGATTCGCCAACGTGACTGCCTCCTGGCTGGGAACCCGGCCAAGGTCGTGGACTTTCACTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGccc                                                                                                                                             <  2:32002/149‑1 (MQ=255)           gCTTTGCGCGAATTGAACCCGCAACTGCGCGTGATTCGCCAACGTGACTGCCTCCTGGCTGGGAACCCGGCCAAGGTCGTGGACTTTCACTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGTTCGGCGCACTGGTGCCCATCGc                                                                                                                                        >  4:34870/1‑149 (MQ=255)                  gcgAATTGAACCCGCAACTGCGCGTGATTCGCCAACGTGACTGCCTCCTGGCTGGGAACCCGGCCAAGGTCGTGGACTTTCACTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGc                                                                                                                                 <  8:314536/149‑1 (MQ=255)                           aCCCGCAACTGCGCGTGATTCGTCAACGTGACTGCCTCCTGGCTGGGAACCCGGCCAAGGTCGTGGACTTTCACTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTg                                                                                                                        >  4:75024/1‑149 (MQ=255)                               gCAACTGCGCGTGATTCGCCAACGTGACTGCCTCCTGGCTGGGAACCCGGCCAAGGTCGTGGACTTTCACTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAAt                                                                                                                    >  4:142610/1‑149 (MQ=255)                                   cTGCGCGTGATTCGCCAACGTGACTGCCTCCTGGCTGGGAACCCGGCCAAGGTCGTGGACTTTCACTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCt                                                                                                                <  8:139799/149‑1 (MQ=255)                                   cTGCGCGTGATTCGCCAACGTGACTGCCTCCTGGCTGGGAACCCGGCCAAGGTCGTGGACTTTCACTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCt                                                                                                                <  7:196659/149‑1 (MQ=255)                                    tGCGCGTGATTCGCCAACGTGACTGCCTCCTGGCTGGGAACCCGGCCAAGGTCGTGGACTTTCACTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTg                                                                                                               <  4:213001/149‑1 (MQ=255)                                      cgggTGATTCGCCAACGTGTCTGCCTCCTGGCTGGGTACCCGGCCAAGGTCGTTGACTTTCACTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGTCAGCGATTCGCTGCAATGGCTgg                                                                                                              <  7:62980/145‑1 (MQ=255)                                       gcgTGATTCGCCAACGTGACTGCCTCCTGGCTGGGAACACGGCCAAGGTCGTGGACTTTCACTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTg                                                                                                               >  1:399319/1‑146 (MQ=255)                                       gcgTGATTCGCCAACGTGACTGCCTCCTGGCTGGGAACACGGCCAAGGTCGTGGACTTTCACTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTg                                                                                                               <  2:399319/146‑1 (MQ=255)                                           gATTCGCCAACGTGACTGCCTCCTGGCTGGGAACCCGGCCAAGGTCGTGGACTTTCACTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCAc                                                                                                                                                     <  4:172458/104‑1 (MQ=255)                                           gATTCGCCAACGTGACTGCCTCCTGGCTGGGAACCCGGCCAAGGTCGTGGACTTTCACTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCAc                                                                                                                                                     >  3:172458/1‑104 (MQ=255)                                                      gTGACTGCCTCCTGGCTGGGAACCCGGCCAAGGTCGTGGACTTTCACTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATc                                                                                             <  3:75024/149‑1 (MQ=255)                                                        gACTGCCTCCTGGCTGGGAACCCGGCCAAGGTCGTGGACTTTCACTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCaa                                                                                                                     <  4:41369/123‑1 (MQ=255)                                                        gACTGCCTCCTGGCTGGGAACCCGGCCAAGGTCGTGGACTTTCACTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCaa                                                                                                                     >  3:41369/1‑123 (MQ=255)                                                         aCTGCCTCCTGGCTGGGAACCCGGCCATGGTCGTGGACTTTCACTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGa                                                                                           <  3:319606/148‑1 (MQ=255)                                                          cttcctcctGGCTGGGAACCCGGCCAAGGTCGTGGACTTTCACTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCTCACTGGAGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGAc                                                                                          <  3:34870/145‑1 (MQ=255)                                                           tGCCTCCTGGCTGGGAACCCGGCCAAGGTCGTGGACTTTCACTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGAccc                                                                                        >  4:314398/1‑149 (MQ=255)                                                                 ctggctggGAACCCGGCCAAGGTCGTGGACTTTCACTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCGCTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGACCCCAGc                                                                                    >  5:402479/1‑147 (MQ=255)                                                                 ctggctggGAACCCGGCCAAGGTCGTGGACTTTCACTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCGCTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGACCCCAGc                                                                                    >  5:408231/1‑147 (MQ=255)                                                                    gctggGAACCCGGCCAAGGTCGTGGACTTTCACTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGACCCCAGCAAGcc                                                                               >  4:195328/1‑149 (MQ=255)                                                                      tggGAACCCGGCCAAGGTCGTGGACTTTCACTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGACCCCAGCAAGCCCg                                                                             >  4:145624/1‑149 (MQ=255)                                                                       gggAACCCGGCCAAGGTCGTGGACTTTCACTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGACCCCAGCAAGCCCgg                                                                            >  8:393008/1‑149 (MQ=255)                                                                          aaCCCGGCCAAGGTCGTGGACTTTCACTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGAcc                                                                                         >  4:407409/1‑133 (MQ=255)                                                                          aaCCCGGCCAAGGTCGTGGACTTTCACTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGACCCCAGCAAGCCCGGCCt                                                                         >  6:335946/1‑149 (MQ=255)                                                                            cccGGCCAAGGTCGTGGACTTTCACTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGACCCCAGCAAGCCCGGCCTGa                                                                       <  7:224840/149‑1 (MQ=255)                                                                                  cAAGGTCGTGGACTTTCACTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGGCCCCAGCAAGCCCGGCCTGAGCCAGt                                                                 >  8:248895/1‑149 (MQ=255)                                                                                      gTCGTGGACTTTCACTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGACCCCAGCAAGCCCGGCCTGAGCCAGtggct                                                             >  3:218552/1‑149 (MQ=255)                                                                                           ggACTTTCACTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGACCCCAGCAAGCCCGGCCTGAGCCAGTGGCTGGcc                                                         >  1:109952/1‑148 (MQ=255)                                                                                                 tCACTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGACCCCAGCAAGCCCGGCCTGAGCCAGTGGCTGGCCGAGtt                                                    <  7:393008/147‑1 (MQ=255)                                                                                                 tCACTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGACCCCAGCAAGCCCGGCCTGAGCCAGTGGCTGGCCGAGTTc                                                   <  4:218552/148‑1 (MQ=255)                                                                                                   aCTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGACCCCAGCAAGCCCGGc                                                                           >  8:139104/1‑122 (MQ=255)                                                                                                   aCTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGACCCCAGCAAGCCCGGc                                                                           <  7:139104/122‑1 (MQ=255)                                                                                                    cTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCGCTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGACCCCAGCAAGCCCGGCCTGAGCCAGTGGCTGGCCGAGTTCGAcc                                               <  6:408231/149‑1 (MQ=255)                                                                                                    cTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCGCTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGACCCCAGCAAGCCCGGCCTGAGCCAGTGGCTGGCCGAGTTCGAcc                                               <  6:402479/149‑1 (MQ=255)                                                                                                     ttCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGACCCCAGCAAGCCCGGCCTGAGCCAGTGGCTGGCCGAGTTCGACCg                                              >  4:133308/1‑149 (MQ=255)                                                                                                     ttCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGACCCCAGCAAGCCCGGCCTGAGCCAGTGGCTGGCCGAGTTCGACCg                                              >  5:234748/1‑149 (MQ=255)                                                                                                       cAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGACCCCAGCAAGCCCGGCCTGAGCCAGtggctgg                                                           >  8:331466/1‑134 (MQ=255)                                                                                                       cAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGACCCCAGCAAGCCCGGCCTGAGCCAGtggctgg                                                           <  7:331466/134‑1 (MQ=255)                                                                                                       cAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGACCCCAGCAAGCCCGGCCTGAGCCAGTGGCTGGCCGAGTTCGACCGc                                             <  5:335946/148‑1 (MQ=255)                                                                                                         aTGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGCCCCCAGCAAGCCCGGCCTGCGCCAGTGGCTGGCCGAGTTCGACCGCATg                                          >  3:335941/1‑149 (MQ=255)                                                                                                          tGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGACCCCAGCAAGCCCGGCCTGAGCCAGTGGCTGGCCGAGTTCGACCGCATGc                                         <  3:142610/149‑1 (MQ=255)                                                                                                              ggCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGACCCCAGCAAGCCCGGCCTGAGCCAGTGGCTGGCCGAGTTCGACCGCATGCTCGa                                     >  4:130656/1‑149 (MQ=255)                                                                                                               gCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGACCCCAGCAAGCCCGGCCTGAGCCAGTGGCTGGCCGAGTTCGACCGCATGCTCGAt                                    <  8:53970/149‑1 (MQ=255)                                                                                                                ccACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGACCCCAGCAAGCCCGGCCTGAGCCAGTGGCTGGCCGAGTTCGACCGCATGCTCGATg                                   <  2:109952/149‑1 (MQ=255)                                                                                                                  aCGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGACCCCAGCAAGCCCGGCCTGAGCCAGTGGCTGGCCGAGTTCGACCGc                                             <  1:6867/137‑1 (MQ=255)                                                                                                                  aCGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGACCCCAGCAAGCCCGGCCTGAGCCAGTGGCTGGCCGAGTTCGACCGc                                             >  2:6867/1‑137 (MQ=255)                                                                                                                       ccTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGACCCCAGCAAGCCCGGCCTGAGCCAGTGGCTGGCCGAGTTCGACCGCATGCTCGATGGCTTcgc                            <  6:234748/149‑1 (MQ=255)                                                                                                                          cGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGACCCCAGCAAGCCCGGCCTGAGCCAGTGGCTGGCCGAGTTCGACCGCATGCTCGATGGCTTcgcg                           >  7:365352/1‑147 (MQ=255)                                                                                                                            gCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCACCAGCGTGATCGACCCCAGCAAGCCCGGCCTGAGCCAGTGGCTGGCCGAGTTc                                                   <  7:277847/121‑1 (MQ=255)                                                                                                                            gCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCACCAGCGTGATCGACCCCAGCAAGCCCGGCCTGAGCCAGTGGCTGGCCGAGTTc                                                   >  8:277847/1‑121 (MQ=255)                                                                                                                            gCACGGTCGCCTGGTCGGCGCACTGGTGACCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCCTGATCGACCACAGCAAGCCCGGCCTGAGCCAGTGGCTGGCCGAGTTAGACCGGATCCTCGATGGCTTCTCGCAgg                       >  8:264524/1‑149 (MQ=255)                                                                                                                                         gTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGACCCCAGCAAGCCCGGCCTGAGCCAGTGGCTGGCCGAGTTCGACCGCATGCTCGATGGCTTCGCGCAGGGCTGACCCCGa            <  3:145624/147‑1 (MQ=255)                                                                                                                                              cgcACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGACCCCAGCAAGCCCGGCCTGAGCCAGTGGCTGGCCGAGTTCGACCGCATGCTCGATGGCTTCGCGCAGGGCTGACCCCGATTTTTTc     <  3:130656/149‑1 (MQ=255)                                                                                                                                                 aCTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGGCCCCAGCAAGCCCGGCCTGAGCCAGTGGCTGGCCGAGTTCGACCGCATGCTCGATGGCTTCGCGCAGGGCTGACCCCGATTTTTTCGCt  <  7:248895/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                     CGCAGTCGAGGCTTTGCGCGAATTGAACCCGCAACTGCGCGTGATTCGCCAACGTGACTGCCTCCTGGCTGGGAACCCGGCCAAGGTCGTGGACTTTCACTTCAATGCCGGCCACGAGCCTCGGCACGGTCGCCTGGTCGGCGCACTGGTGCCCATCGCCGGCAGCGATTCGCTGCAATGGCTGGATATCTCCAGCGTGATCGACCCCAGCAAGCCCGGCCTGAGCCAGTGGCTGGCCGAGTTCGACCGCATGCTCGATGGCTTCGCGCAGGGCTGACCCCGATTTTTTCGCT  >  NC_002947/3510548‑3510840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑