Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,551,209 G→C 8.7% E121Q (GAG→CAG)  PP_3135 → glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,551,2090GC 8.7% 175.2 / 4.7 55E121Q (GAG→CAG) PP_3135glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base G (23/27);  new base C (2/3);  total (25/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

CGGGCCAACAACCAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGCAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCCACACCGCTCAATGTGTTCGTCGGGCGTACCGGGTTGGGGCGGTTCTTCCCAGGGGTGAAGATGGTCGACGACATGAACTGGGACCACGCCTCGGTGCGCGA  >  NC_002947/3551062‑3551354
                                                                                                                                                   |                                                                                                                                                 
cGGGCCAACAACCAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGCAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGa                                                                                                                                                  <  6:150443/149‑1 (MQ=255)
 gggCCAACAACCAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGCAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAg                                                                                                                                                 <  6:322091/149‑1 (MQ=255)
     caacaaCCAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGCAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTc                                                                                                                                             >  2:395949/1‑149 (MQ=255)
           ccAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGCAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTgg                                                                                                                                                   <  4:36394/137‑1 (MQ=255)
           ccAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGCAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTgg                                                                                                                                                   >  3:36394/1‑137 (MQ=255)
            cAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGCAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATgg                                                                                                                                      >  6:79635/1‑149 (MQ=255)
             aGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGCAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGc                                                                                                                                     <  5:300736/149‑1 (MQ=255)
                      cgcACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGCAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGCA‑GGTCGCCATCGCGAccctccag                                                                                                                           <  2:244723/149‑7 (MQ=255)
                       gcACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGCAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGCA‑GGTCGCCATCGCGAccctccagg                                                                                                                          <  8:52781/149‑8 (MQ=255)
                            tCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGCAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGt                                                                                                                      >  5:52280/1‑149 (MQ=255)
                               aGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGCAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGt                                                                                                                   <  4:342765/149‑1 (MQ=255)
                                 ggCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGCAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCg                                                                                                                 <  8:10672/149‑1 (MQ=255)
                                 ggCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGCAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCg                                                                                                                 >  7:142669/1‑149 (MQ=255)
                                                  gctCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGCAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCCACAcca                                                                                                 >  2:290330/1‑147 (MQ=255)
                                                   ctCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGCAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCCACACCGc                                                                                                <  4:341824/148‑1 (MQ=255)
                                                    tCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGCAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCg                                                                                                                 <  4:242147/130‑1 (MQ=255)
                                                    tCAATACAGATGCCTTCGTTGCCGCCGACTCGCTGCAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCg                                                                                                                 >  3:242147/1‑130 (MQ=255)
                                                       aTACCGATGCCTTCGTTGCCGCCGACTCGCTGCAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCCACACCGCTCAAt                                                                                           <  1:301358/149‑1 (MQ=255)
                                                           cGATGCCTTCGTTGCCGCCGACTCGCTGCAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCCACACCGCTCAATgtgt                                                                                       >  3:234868/1‑149 (MQ=255)
                                                           cGATGCCTTCGTTGCCGCCGACTCGCTGCAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCCACACCGCTCAATgtgt                                                                                       >  7:92739/1‑149 (MQ=255)
                                                           cGATGCCTTCGTTGCCGCCGACTCGCTGCAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCCACACCACTCAATgtgt                                                                                       <  1:290330/149‑1 (MQ=255)
                                                                 cTTCGTTGCCGCCGACTCGCTGCAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCCACACCGCTCAATGTGTtcgtcg                                                                                 >  5:5486/1‑149 (MQ=255)
                                                                  ttCGTTGCCGCCGACTCGCTGCAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCCACACCGCTCAATGTGTTCGTCgg                                                                                >  6:346666/1‑149 (MQ=255)
                                                                  ttCGTTGCCGCCGACTCGCTGCAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCCACACCGCTCAATGTGTTCGTCgg                                                                                >  8:332863/1‑149 (MQ=255)
                                                                    cGTTGCCGCCGACTCGCTGCAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCCACACCGCTCAATGTGTTCGTCggg                                                                               <  8:142669/148‑1 (MQ=255)
                                                                    cGTTGCCGCCGACTCGCTGCAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCCACACCGCTCAATGTGTTCGTCgg                                                                                <  4:234868/147‑1 (MQ=255)
                                                                     gTTGCCGCCGACTCGCTGCAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCCACACCGCTCAATGTGTTCGTCGGGc                                                                              <  1:163268/148‑1 (MQ=255)
                                                                         ccgccgACTCGCTGCAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCCACACCGCTCAATGTGTTCGTCGGGCGTAcc                                                                         >  3:402643/1‑149 (MQ=255)
                                                                              gACTCGCTGCAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCCACACCGCTCAATGTGTTCGTCGGGCGTACCGGGtt                                                                    <  6:52280/149‑1 (MQ=255)
                                                                               aCTCGCTGCAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCCACACCGCTCAATGTGTTCGTCGGGCGTACCGGGTTg                                                                   <  7:332863/149‑1 (MQ=255)
                                                                                cTCGCTGCAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCCACACCGCTCAATGTGTTCGTCGGGCGTACCGGGTTgg                                                                  >  3:312267/1‑149 (MQ=255)
                                                                                   gCTGCAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGa                                                                                                                                                  <  2:26511/66‑1 (MQ=255)
                                                                                   gCTGCAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGa                                                                                                                                                  >  1:26511/1‑66 (MQ=255)
                                                                                       cAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTccc                                                                                                        <  2:95961/104‑1 (MQ=255)
                                                                                       cAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCCACACCGCTCAATGTGTTCGTCGGGCGTACCGGGTTGGGGCgg                                                             <  5:346666/147‑1 (MQ=255)
                                                                                       cAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCCACACCGCTCAATGTGTTCGTCGGGCGTACCGGGTTGGGGCGGt                                                            <  8:92739/148‑1 (MQ=255)
                                                                                       cAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTAGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTccc                                                                                                        >  1:95961/1‑104 (MQ=255)
                                                                                        aaaaaaCGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGGACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCCACACCGCTCAATGTGTTCGTCGGGCGTACAGGGTTGGGGCGGttc                                                          >  4:104431/1‑149 (MQ=255)
                                                                                            aaCGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCCACACCGCTCAATGTGTTCGTCGGGCGTACCGGGTTGGGGCGGTTCTTcc                                                      >  3:130038/1‑149 (MQ=255)
                                                                                                   gACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCCACACCGCTCAATGTGTTCGTCGGGCGTACCGGGTTGGGGCGGTTCTTCCCAgggg                                                <  3:391879/148‑1 (MQ=255)
                                                                                                     cTACAAGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGCACGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCAACACCGCTCAATGTCTTAGTCGGCCGTACCGGGATGGGGAGGTTCTTCCCAgcgctc                                              >  2:114548/1‑143 (MQ=255)
                                                                                                        cATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCCACACCGCTCAATGTGTTCGTCGGGCGTACCGGGTTGGGGCGGTTCTTCCCAGGGGTg                                              >  1:393682/1‑145 (MQ=255)
                                                                                                             aCGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCCACACCGCTCAATGTGTTCGTCGGGCGTACCGGGTTGGGGCGGTTCTTCCCAGGGGTGAAGATGGTc                                     <  1:395949/149‑1 (MQ=255)
                                                                                                               gCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCCACACCGCTCAAt                                                                                           <  8:66955/93‑1 (MQ=255)
                                                                                                               gCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCCACACCGCTCAAt                                                                                           >  7:66955/1‑93 (MQ=255)
                                                                                                               gCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTACCCACACCGCTCAATGTGTTCGTCGGGCGTACCGGGTTGGGGCGGTTCTTCCCAGGGGTGAAGATGGTcg                                    >  4:341541/1‑148 (MQ=255)
                                                                                                                 ccACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCCACACCGCTCAATGTGTTCGTCGGGCGTACCGGGTTGGGGCGGTTCTTCCCAGGGGTGAAGATGGTcgac                                  >  1:116224/1‑148 (MQ=255)
                                                                                                                   aCCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCCACACCGCTCAATGTGTTCGTCGGGCGTACCGGGTTGGGGCGGTTCTTCCCAGGGGTGAAg                                           <  5:62025/137‑1 (MQ=255)
                                                                                                                   aCCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCCACACCGCTCAATGTGTTCGTCGGGCGTACCGGGTTGGGGCGGTTCTTCCCAGGGGTGAAg                                           >  6:62025/1‑137 (MQ=255)
                                                                                                                   aCCCGGAGTGCGGCGTACTGGGGGTGCGCCTGCAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCCACACCGCTCAATGTGTTCGTCGGGCGTACCgg                                                                       >  2:220002/1‑109 (MQ=255)
                                                                                                                   aCCCGGAGTGCGGCGTACTGGGGGTGCGCCTGCAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCCACACCGCTCAATGTGTTCGTCGGGCGTACCgg                                                                       <  1:220002/109‑1 (MQ=255)
                                                                                                                    cccGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCCACACCGCTCAATGTGTTCGTCGGGCGTACCGGGTTGGGGCGGTTCTTCCCAGGGGTGAAGATGGTCGACGACa                              <  2:393682/149‑1 (MQ=255)
                                                                                                                     ccGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCa                                                                                                                        <  7:377519/58‑1 (MQ=255)
                                                                                                                     ccGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCa                                                                                                                        >  8:377519/1‑58 (MQ=255)
                                                                                                                                 gTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCCACACCGCTCAATGTGTTCGTCGGGCGTACCGGGTTGGGGCGGTTCTTCCCAGGGGTGAAGATGGTCGACGACATGAACTGGGACCa                 <  6:5486/149‑1 (MQ=255)
                                                                                                                                            gcgcCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCCACACCGCTCAATGTGTTCGTCGGGCGTACCGGGTTGGGGCGGTTCTTCCCAGGGGTGAAGATGGTCGACGACATGAACTGGGACCACGCCTCGGTgc      <  4:312267/149‑1 (MQ=255)
                                                                                                                                                cTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCCACACCGCTCAATGTGTTCGTCGGGCGTACCGGGTTGGGGCGGTTCTTCCCAGGGGTGAAGATGGTCGACGACATGAACTGGGACCACGCCTCGGTGCGCGa  <  3:68012/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CGGGCCAACAACCAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGCAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGCGACCTGCAGCCCAGTTGTCGCTACTTCCCCACACCGCTCAATGTGTTCGTCGGGCGTACCGGGTTGGGGCGGTTCTTCCCAGGGGTGAAGATGGTCGACGACATGAACTGGGACCACGCCTCGGTGCGCGA  >  NC_002947/3551062‑3551354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑