Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,586,375 T→C 7.2% V190A (GTG→GCG)  benK → benzoate MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,586,3750TC 7.2% 153.6 / 2.2 55V190A (GTG→GCG) benKbenzoate MFS transporter
Reads supporting (aligned to +/- strand):  ref base T (27/24);  new base C (2/2);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCCTGCGCAGCACGCTGGTGGCGATCATGTTCAGTGGTTATTCGCTGGGCGGCATGCTGTCGGCAGGTGTCGGCATCTTCATGCTGCCGCGTTTTGGCTGGGAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTGGTACGCCAAGGCCGTATCGAGGAAGCACGCACATTGCTCAAACGGCTGGA‑CCCGGACTGCGATGTCCAGGCCGATGATGTGCTTCAGGCGGCCGACCGCAAAGGCAGCGGC  >  NC_002947/3586227‑3586523
                                                                                                                                                    |                                                                                                                                                     
cgcCTGCGCAGCACGCTGGTGGCGATCATGTTCAGTGGTTATTCGCTGGGCGGCATGCTGTCGGCAGGTGTCGGCATCTTCATGCTGCCGCGTTTTGGCTGGGAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGt                                                                                                                                                       <  7:358364/149‑1 (MQ=255)
    tGCGCAGCACGCTGGTGGCGATCATGTTCAGTGGTTATTCGCTGGGCGGCATGCTGTCGGCAGGTGTCGGCATCTTCATGCTGCCGCGTTTTGGCTGGGAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGAt                                                                                                                                                    >  2:178719/1‑148 (MQ=255)
      cgcAGCACGCTGGTGGCGATCATGTTCAGTGGTTATTCGCTGGGCGGCATGCTGTCGGCAGGGGTCGGCATCTTCATGCTGCCGCGTTTTGGCTGGGAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATtct                                                                                                                                                 <  8:318351/149‑1 (MQ=255)
       gcagcaCGCTGGTGGCGATCATGTTCAGTGGTTATTCGCTGGGCGGCATGCTGTCGGCAGGTGTCGGCATCTTCATGCTGCCGCGTTTTGGCTGGGAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATtctc                                                                                                                                                <  5:319557/149‑1 (MQ=255)
         agcaCGCTGGTGGCGATCATGTTCAGTGGTTATTCGCTGGGCGGCATGCTGTCGGCAGGTGTCGGCATCTTCATGCTGCCGCGTTTTGGCTGGGAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTcta                                                                                                                                              >  5:229457/1‑149 (MQ=255)
           caCGCTGGTGGCGATCATGTTCAGTGGTTATTCGCTGGGCGGCATGCTGTCGGCAGGTGTCGGCATCTTCATGCTGCCGCGTTTTGGCTGGGAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTctact                                                                                                                                            <  7:217796/149‑1 (MQ=255)
           caCGCTGGTGGCGATCATGTTCAGTGGTTATTCGCTGGGCGGCATGCTCTCGGCAGGTGTCGGCATCTTCATGCTGCCGCGTTTTGGCTGGGAGTCTATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTctac                                                                                                                                             >  6:227149/1‑148 (MQ=255)
             cGCTGGTGGCGATCATGTTCAGTGGTTATTCGCTGGGCGGCATGCTGTCGGCAGGTGTCGGCATCTTCATGCTGCCGCGTTTTGGCTGGGAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTCtactac                                                                                                                                          <  4:136867/149‑1 (MQ=255)
                  gtggCGATCATGTTCAGTGGTTATTCGCTGGGCGGCATGCTGTCGGCAGGTGTCGGCATCTTCATGCTGCCGCGTTTTGGCTGGGAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTCTACTAcctgc                                                                                                                                      <  3:209835/148‑1 (MQ=255)
                   tggCGATCATGTTCAGTGGTTATTCGCTGGGCGGCATGCTGTCGGCAGGTGTCGGCATCTTCATGCTGCCGCGTTTTGGCTGGGAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTCTACTAcctgcct                                                                                                                                    >  3:113524/1‑149 (MQ=255)
                       gATCATGTTCAGTGGTTATTCGCTGGGCGGCATGCTGTCGGCAAGTGTCGGCATCTTCATGCTGCCGCGTTTTGGCTGGGAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCTGTGATTCTCTACTACCTGCCTGAAt                                                                                                                                >  1:285116/1‑149 (MQ=255)
                            tGTTCAGTGGTTATTCGCTGGGCGGCATGCTGTCGGCAGGTGTCGGCATCTTCATGCTGCCGCGTTTTGGCTGGGAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTCTACTACCTGCCTGAATCCATc                                                                                                                           <  6:281601/149‑1 (MQ=255)
                                cAGTGGTTTTTCGCTGGGCGGCATGCTGTCGGCAGGTGTCGGCATCTTGATGCTGCCGCGTTTTGGCTGGGAGTCGATGTTCTTCGCCTCAGCGGTGCCACTTCTGCTGTTACCGGTGATTCTCTACTACCTGCCTGAATCCATCGGc                                                                                                                        <  7:132409/148‑1 (MQ=255)
                                  gTGGTTATTCGCTGGGCGGCATGCTGTCGGCAGGTGTCGGCATCTTCATGCTGCCGCGTTTTGGCTGGGAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTCTACTACCTGCCTGAATCCATCGGCTTc                                                                                                                     <  6:229457/149‑1 (MQ=255)
                                   tGGTTATTCGCTGGGCGGCATGCTGTCGGCAGGTGTCGGCATCTTCATGCTGCCGCGTTTTGGCTGGGAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTctac                                                                                                                                             <  4:360152/124‑1 (MQ=255)
                                   tGGTTATTCGCTGGGCGGCATGCTGTCGGCAGGTGTCGGCATCTTCATGCTGCCGCGTTTTGGCTGGGAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTctac                                                                                                                                             >  3:360152/1‑124 (MQ=255)
                                     gTTATTCGCTGGGCGGCATGCTGTCGGCAGGTGTCGGCATCTTCATGCTGCCGCGTTTTGGCTGGGAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTg                                                                                                                  >  3:377563/1‑149 (MQ=255)
                                     gTTATTCGCTGGGCGGCATGCTGTCGGCAGGTGTCGGCATCTTCATGCTGCCGCGTTTTGGCTGGGAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGCGATtctc                                                                                                                                                <  8:226053/119‑1 (MQ=255)
                                     gTTATTCGCTGGGCGGCATGCTGTCGGCAGGTGTCGGCATCTTCATGCTGCCGCGTTTTGGCTGGGAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGCGATtctc                                                                                                                                                >  7:226053/1‑119 (MQ=255)
                                        aTTCGCTGGGCGGCATGCTGTCGGCAGGTGTCGGCATCTTCATGCTGCCGCGTTTTGGCTGGGAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGCGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTGGTa                                                                                                               >  4:226651/1‑149 (MQ=255)
                                            gCTGGGCGGCATGCTGTCGGCAGGTGTCGGCATCTTCATGCTGCCGCGTTTTGGCTGGGAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTGGTACGc                                                                                                            >  8:271426/1‑148 (MQ=255)
                                                 gcggcATGCTGTCGGCAGGTGTCGGCATCTTCATGCTGCCGCGTTTTGGCTGGGAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTGGTACGCCAAGGc                                                                                                      >  6:139705/1‑149 (MQ=255)
                                                  cggcATGCTGTCGGCAGGTGTCGGCATCTTCATGCTGCCGCGTTTTGGCTGGGAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTGGt                                                                                                                <  6:97909/138‑1 (MQ=255)
                                                  cggcATGCTGTCGGCAGGTGTCGGCATCTTCATGCTGCCGCGTTTTGGCTGGGAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTGGt                                                                                                                >  5:97909/1‑138 (MQ=255)
                                                        gCTGTCGGCAGGTGTCGGCATCTTCATGCTGCCGCGTTTTGGCTGGGAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTCTACTAcctgc                                                                                                                                      >  5:202972/1‑110 (MQ=255)
                                                        gCTGTCGGCAGGTGTCGGCATCTTCATGCTGCCGCGTTTTGGCTGGGAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTCTACTAcctgc                                                                                                                                      <  6:202972/110‑1 (MQ=255)
                                                            tCGGCAGGTGTCGGCATCTTCATGCTGCCGCGTTTTGGCTGGGAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGCGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTGGTACGCCAAGGCCGTATCGAg                                                                                             <  3:226651/147‑1 (MQ=255)
                                                                        ggCATCTTCACGCTGCCGCGTTTTGGCTGGGAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTGGTACGCCAAGGCCGTATCGAGGAAGCACGCACAtt                                                                               >  3:305957/1‑149 (MQ=255)
                                                                                 aTGCTGCCGCGTTTTGGCTGGGAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTGGTACGCCAAGGCCGTATCGAGGAAGCACGCACATTGCTCAAAc                                                                       <  7:271426/148‑1 (MQ=255)
                                                                                 aTGCTGCCGCGTTTTGGCTGGGAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCAGTGATTCTCTACTACCTGCCTGa                                                                                                                                  <  4:63054/89‑1 (MQ=255)
                                                                                 aTGCTGCCGCGTTTTGGCTGGGAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCAGTGATTCTCTACTACCTGCCTGa                                                                                                                                  >  3:63054/1‑89 (MQ=255)
                                                                                       ccGCGTTTTGGCTGGGAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTGGTACGCCAAGGCCGTATCGa                                                                                              <  1:20570/119‑1 (MQ=255)
                                                                                       ccGCGTTTTGGCTGGGAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTGGTACGCCAAGGCCGTATCGa                                                                                              >  2:20570/1‑119 (MQ=255)
                                                                                       ccGCGTTTTGGCTGGGAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTGGTACGCCAAGGCCGTATCGAGGAAGCACGCACATTGCTCAAACGGCTg                                                                  <  3:297773/147‑1 (MQ=255)
                                                                                                 gCTGGGAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTGGTACGCCAAGGCCGTATCGAGGAAGCACGCACATTGCTCAAACGGCTGGA‑CCCGGACTg                                                      <  4:377563/148‑1 (MQ=255)
                                                                                                 gCTGGGAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTGGTACGCCAAGGCCGTATCGAGGAAGCACGCACATTGCTCAAACGGCTGGA‑CCCGGACTGc                                                     >  4:226755/1‑149 (MQ=255)
                                                                                                     ggAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTGGTACGCCAAGGCCGTATCGAGGAAGCACGCACATTGCTCAAACGGCTGGA‑CCCGGACTGCGa                                                   <  5:139705/147‑1 (MQ=255)
                                                                                                     ggAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTGGTACGCCAAGGCCGTATCGAGGAAGCACGCACATTGCTCAAACGGCTGGA‑CCCGGACTGCGATg                                                 >  6:117299/1‑149 (MQ=255)
                                                                                                           gATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTGGTACGCCAAGGCCGTATCGAGGAAGCACGCACATTGCTCAAACGGCt                                                                   <  4:3337/126‑1 (MQ=255)
                                                                                                           gATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTGGTACGCCAAGGCCGTATCGAGGAAGCACGCACATTGCTCAAACGGCt                                                                   >  3:3337/1‑126 (MQ=255)
                                                                                                           gATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTGGTACGCCAAGGCCGTATCGAGGAAGCACGCACATTGCTCAAACGGCTGGA‑CCCGGACTGCGATGTCCAgg                                           >  5:279914/1‑149 (MQ=255)
                                                                                                           gATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTGGTACGCCAAGGCCGTATCGAGGAAGCACGCACATTGCTCAAACGGCTGGA‑CCCGGACTGCGATGTCCAgg                                           <  4:305957/149‑1 (MQ=255)
                                                                                                           gATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTGGTACGCCAAGGCCGTATCGAGGAAGCACGCACATTGCTCAAACGGCTGGA‑CCCGGACTGCGATGTCCAgg                                           >  1:9351/1‑149 (MQ=255)
                                                                                                            aTGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTGGTACGCCAAGGCCGTATCGAGGAAGCACGCACATTGCTCAAACGGCTGGA‑CCCGGACTGCGATGTCCAGGc                                          >  6:309167/1‑149 (MQ=255)
                                                                                                                  ttcGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTGGTACGCCAAGGCCGTATCGAGGAAGCACGCACATTGCTCAAACGGCTGGA‑CCCGGACTGCGATGTCCAGGCCgatga                                    >  4:372569/1‑149 (MQ=255)
                                                                                                                   tcGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTGGTACGCCAAGGCCGTATCGAGGAAGCACGCACATTGCTCAAACGGCTGGA‑CCCGGACTGCGATGTCCAGGCCgatgat                                   >  1:270218/1‑149 (MQ=255)
                                                                                                                    cgccgcAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTGGTACGCCAAGGCCGTATCGAGGAAGCACGCACATTGCTCAAACGGCTGGA‑CCCGGACTGCGATGTCCAGGCCg                                        <  5:162221/143‑1 (MQ=255)
                                                                                                                    cgccgcAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTGGTACGCCAAGGCCGTATCGAGGAAGCACGCACATTGCTCAAACGGCTGGA‑CCCGGACTGCGATGTCCAGGCCg                                        >  6:162221/1‑143 (MQ=255)
                                                                                                                      ccgcAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTGGTACGCCAAGGCCGTATCGAGGAAGCACGCACATTGCTCAAACGGCTGGA‑CCCGGACTGCGATGTCCAGGCCGATGAtgtg                                >  1:21356/1‑149 (MQ=255)
                                                                                                                       cgcAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTGGTACGCCAAGGCCGTATCGAGGAAGCACGCACATTGCTCAAACGGCTGGA‑CCCGGACTGCGATGTCCAGGCCGATGATGTGc                               <  3:226755/149‑1 (MQ=255)
                                                                                                                               tGCCACTGCTGCTGTTACCGGTGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTGGTACGCCAAGGCCGTATCGAGGAAGCACGCACATTGCTCAAACGGCTGGA‑CCCTGACTGCGATGTCCAGGCCGATGATGTGCTTCAggcg                       <  2:285116/149‑1 (MQ=255)
                                                                                                                                   aCTGCTGCTGTTACCGGTGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTGGTACGCCAAGGCCGTATCGAGGAAGCACGCACATTGCTCAAACGGCTGGA‑CCCGGACTGCGATGTCCAGGCCGATGATGTGCTTCAg                          <  6:312330/142‑1 (MQ=255)
                                                                                                                                   aCTGCTGCTGTTACCGGTGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTGGTACGCCAAGGCCGTATCGAGGAAGCACGCACATTGCTCAAACGGCTGGA‑CCCGGACTGCGATGTCCAGGCCGATGATGTGCTTCAg                          >  5:312330/1‑142 (MQ=255)
                                                                                                                                   aCTGCTGCTGTTACCGGTGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTGGTACGCCAAGGCCGTATCGAGGAAGCACGCACATTGCTCAAACGGCTGGA‑CCCGGACTGCGATGTCCAGGCCGATGATGTGCTTCAGGCGGcc                    >  2:96229/1‑148 (MQ=255)
                                                                                                                                             ttACCGGTGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTGGTACGCCAAGGCCGTATCGAGGAAGCACGCACATTGCTCAAACGGCTGGACCCCGGACTGCGATGTCCAGGCCGATGATGTGCTTCAGGCGGCCGACCGCaaa           <  1:96229/148‑1 (MQ=255)
                                                                                                                                             ttACCGGTGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTAGTACGCCAAGGCCGTATCGAGGAAGCACGCACATTGCTCAAACGGCTGGA‑CCCGGACTGCGATGTCCAGGCCGATGATGTGCTTCAGGCGGCCGACCGCAAAgg         >  6:346198/1‑149 (MQ=255)
                                                                                                                                                    tGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTGGTACGCCAAGGCCGTATCGAGGAAGCACGCACATTGCTCAAACGGCTGGA‑CCCGGACTGCGATGTCCAGGCCGATGATGTCCTTCAGGCGGCCGACCGCAAAGGCAgcggc  <  1:36039/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGCCTGCGCAGCACGCTGGTGGCGATCATGTTCAGTGGTTATTCGCTGGGCGGCATGCTGTCGGCAGGTGTCGGCATCTTCATGCTGCCGCGTTTTGGCTGGGAGTCGATGTTCTTCGCCGCAGCGGTGCCACTGCTGCTGTTACCGGTGATTCTCTACTACCTGCCTGAATCCATCGGCTTCCTGGTACGCCAAGGCCGTATCGAGGAAGCACGCACATTGCTCAAACGGCTGGA‑CCCGGACTGCGATGTCCAGGCCGATGATGTGCTTCAGGCGGCCGACCGCAAAGGCAGCGGC  >  NC_002947/3586227‑3586523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑