Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,590,325	G→C	11.4%	R282R (CGG→CGC)	nicP‑I →	porin‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,590,325	0	G	C	11.4%	172.4 / 10.6	61	R282R (CGG→CGC)	nicP‑I	porin‑like protein
Reads supporting (aligned to +/- strand):  ref base G (26/28);  new base C (2/5);  total (28/33)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01

AACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAGGTA  >  NC_002947/3590177‑3590469                                                                                                                                                     |                                                                                                                                                 aacaaGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCgg                                                                                                                                                  >  4:72386/1‑149 (MQ=255)    aaGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGGACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCAt                                                                                                                                               >  8:132201/1‑149 (MQ=255)            gttgGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGTGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGc                                                                                                                                        <  7:137796/148‑1 (MQ=255)            gttgGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCggg                                                                                                                                         <  7:301552/147‑1 (MQ=255)             ttgGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGCCATGCCGGGca                                                                                                                                       <  2:177949/148‑1 (MQ=255)               ggCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGCCATGCCGGGcagt                                                                                                                                     <  2:257347/148‑3 (MQ=255)                   tGAAGCACAGCGAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTc                                                                                                                                >  2:184927/1‑149 (MQ=255)                   tGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGCCATGCCGGGcagt                                                                                                                                     >  4:305181/1‑142 (MQ=255)                   tGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGCCATGCCGGGcagt                                                                                                                                     <  3:305181/144‑3 (MQ=255)                        cacaGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGCCATGCCGGGcagt                                                                                                                                     <  1:188022/139‑3 (MQ=255)                        cacaGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGCCATGCCGGGcagt                                                                                                                                     >  2:188022/1‑137 (MQ=255)                         acaGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGcc                                                                                                                          >  1:308747/1‑149 (MQ=255)                          caGCAAACCCGTCGGCGACTGGATACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCg                                                                                                                         >  2:196803/1‑149 (MQ=255)                               aaCCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCa                                                                                                                                                <  1:147235/121‑1 (MQ=255)                               aaCCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCa                                                                                                                                                >  2:147235/1‑121 (MQ=255)                                aCCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTAc                                                                                                                    >  8:282566/1‑148 (MQ=255)                                   cGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAgg                                                                                                                <  3:72386/149‑1 (MQ=255)                                            cTGGATACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATg                                                                                                       <  1:196803/149‑1 (MQ=255)                                             tGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATgg                                                                                                      <  7:282566/149‑1 (MQ=255)                                               ggTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGc                                                                                                     <  6:277131/148‑1 (MQ=255)                                                    tCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAgg                                                                                                                >  1:63984/1‑132 (MQ=255)                                                    tCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAgg                                                                                                                <  2:63984/132‑1 (MQ=255)                                                    tCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGc                                                                                               >  6:214720/1‑149 (MQ=255)                                                      aGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGc                                                                                               <  7:132201/147‑1 (MQ=255)                                                       gTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCg                                                                                            >  5:84336/1‑149 (MQ=255)                                                       gTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCg                                                                                            >  5:8927/1‑149 (MQ=255)                                                            aaCCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTcc                                                                                        >  7:277613/1‑148 (MQ=255)                                                              ccTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCAc                                                                                     >  1:379794/1‑149 (MQ=255)                                                                  ggTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACgg                                                                                   <  8:124689/147‑1 (MQ=255)                                                                             cgagcgTGAGACAGGCGAAGCGCAGCTGGGCAACATCGACAACCACGCGTTCTTCTCGCTGTTCACTGCCCGCCATGGCGGGCACAGATTCCATGCCGGCTACCACGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACa                                                                      <  6:243031/146‑1 (MQ=255)                                                                              gcgcgTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGATCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACAt                                                                     >  1:91931/1‑149 (MQ=255)                                                                                       cAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCTGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGtt                                                            <  5:4232/149‑1 (MQ=255)                                                                                       cAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGtt                                                            <  6:84336/149‑1 (MQ=255)                                                                                        aaGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTg                                                           <  8:277613/149‑1 (MQ=255)                                                                                         aGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTgg                                                          <  5:134963/149‑1 (MQ=255)                                                                                          ggCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCAt                                                                                                            <  6:369675/98‑1 (MQ=255)                                                                                          ggCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCAt                                                                                                            >  5:369675/1‑98 (MQ=255)                                                                                              aTGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAAc                                                     <  5:214720/149‑1 (MQ=255)                                                                                              aTGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAAc                                                     >  2:278677/1‑149 (MQ=255)                                                                                                gcgcTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAAc                                                     >  4:392886/1‑147 (MQ=255)                                                                                                  gctgctGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGc                                                        >  4:290247/1‑142 (MQ=255)                                                                                                  gctgctGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGc                                                        <  3:290247/142‑1 (MQ=255)                                                                                                        gggCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGa                                           >  1:246204/1‑149 (MQ=255)                                                                                                          gCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGt                                                                                 <  8:192650/109‑1 (MQ=255)                                                                                                          gCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGt                                                                                 >  7:192650/1‑109 (MQ=255)                                                                                                            aaCATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTa                                       >  5:327080/1‑149 (MQ=255)                                                                                                             aCATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTAt                                      <  2:308747/149‑1 (MQ=255)                                                                                                               aTCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTAt                                      >  3:117163/1‑147 (MQ=255)                                                                                                                     aaCCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGc                              <  1:278677/149‑1 (MQ=255)                                                                                                                           gCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTacac                        <  3:392886/149‑1 (MQ=255)                                                                                                                                   ctcGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGaa                 <  6:8927/148‑1 (MQ=255)                                                                                                                                   ctcGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAAc                >  2:321271/1‑149 (MQ=255)                                                                                                                                      gCTGTTCTCTGCCCGGCATGGCGGGCACAGCTTCCATGGCGGCTACGAGGGCATTTATGGCGTTAGCCCGTTGCCACGGGTATTTGCCATCATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTTCCTACACCGACGAACGCt             <  2:91931/149‑1 (MQ=255)                                                                                                                                      gCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCt             <  1:321271/149‑1 (MQ=255)                                                                                                                                       cTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTc            <  2:379794/149‑1 (MQ=255)                                                                                                                                         gTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCg           >  3:3207/1‑148 (MQ=255)                                                                                                                                          ttCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTa         >  4:343050/1‑149 (MQ=255)                                                                                                                                            cACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTAc        <  1:184927/148‑1 (MQ=255)                                                                                                                                              cTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAg     <  5:343106/149‑1 (MQ=255)                                                                                                                                                 cccGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCg                                                              >  7:324401/1‑89 (MQ=255)                                                                                                                                                 cccGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCg                                                              <  8:324401/89‑1 (MQ=255)                                                                                                                                                 cccGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAGGTa  >  5:101602/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                 AACAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAGGTA  >  NC_002947/3590177‑3590469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑