Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,591,577	T→A	7.7%	L258* (TTG→TAG) ‡	PP_3169 →	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,591,577	0	T	A	7.7%	198.5 / 5.4	65	L258* (TTG→TAG) ‡	PP_3169	membrane protein
Reads supporting (aligned to +/- strand):  ref base T (30/30);  new base A (2/3);  total (32/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.33e-01

GTCTGGCGGGTGCTGGCCAAGACCCCGAATGGCGATTACTACGAAGGCATCAGCAGCGTGCTCGACCAGCAACCACCCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGT  >  NC_002947/3591429‑3591722                                                                                                                                                     |                                                                                                                                                  gTCTGGCGGGTGCTGGCCAAGACCCCGATTGGCGATTACTACGAAGGCATCAGCAGCGTGCTCGACCAGCAACCACCCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCtt                                                                                                                                                   >  6:55227/1‑149 (MQ=255) gTCTGGCGGGTGCTGGCCAAGACCCCGAATGGCGATTACTACGAAGGCATCAGCAGCGTGCTCGACCAGCAACCACCCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCtt                                                                                                                                                   <  7:17628/149‑1 (MQ=255)  tCTGGCGGGTGCTGGCCAAGACCCCGAATGGCGATTACTACGAAGGCATCAGCAGCGTGCTCGACCAGCAACCACCCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTg                                                                                                                                                  >  5:105058/1‑149 (MQ=255)      gCGGGTGCTGGCCAAGACCCCGAATGGCGATTACTACGAAGGCATCAGCAGCGTGCTCGACCAGCAGCCACCCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTgcgc                                                                                                                                               <  1:197753/148‑1 (MQ=255)        gggTGCTGGCCAAGACCCCGAATGGCGATTACTACGAAGGCATCAGCAGCGTGCTCGACCAGCAACCACCCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTgg                                                                                                                                            <  4:197480/149‑1 (MQ=255)             cTGGCCAAGACCCCGAATGGCGATTACTACGAAGGCATCAGCAGCGTGCTCGACCAGCAACCACCCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCa                                                                                                                                        <  1:220558/148‑1 (MQ=255)              tGGCCAAGACCCCGAATGGCGATTACTACGAAGGCATCAGCAGCGTGCTCGACCAGCAACCACCCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCAcc                                                                                                                                      >  3:372995/1‑149 (MQ=255)                gCCAAGACCCCGAATGGCGATTACTACGAAGGCATCAGCAGCGTGCTCGACCAGCAACCACCCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGa                                                                                                                                    >  7:220994/1‑149 (MQ=255)                   aaGACCCCGAATGGCGATTACTACGAAGGCATCAGCAGCGTGCTCGACCAGCAACCACCCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGa                                                                                                                                 <  3:253796/149‑1 (MQ=255)                     gACCCCGAATGGCGATTACTACGAAGGCATCAGCAGCGTGCTCGACCAGCAACCACCCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACt                                                                                                                               <  5:55227/149‑1 (MQ=255)                         ccGAATGGCGATTACTACGAAGGCATCAGCAGCGTGCTCGACCAGCAACCACCCGAAATGCAACGCTTGTCGAGCAACCTGGAAATGGCGCAAGCAATGAAAGATGACACGTTGCACCAGCGCTTGCGATGGTTAAACGATGACTGGCt                                                                                                                           >  7:181720/1‑149 (MQ=255)                          cGAATGGCGATTACTACGAAGGCATCAGCAGCGTGCTCGACCAGCAACCACCCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACt                                                                                                                               >  1:298102/1‑144 (MQ=255)                          cGAATGGCGATTACTACGAAGGCATCAGCAGCGTGCTCGACCAGCAACCACCCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACt                                                                                                                               <  2:298102/144‑1 (MQ=255)                                        tacGAAGGCATCAGCAGCGTGCTCGACCAGCAACCACCCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGCTGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGAGCAGAt                                                                                                            >  2:49338/1‑149 (MQ=255)                                        tacGAAGGCATCAGCAGCGTGCTCGACCAGCAACCACCCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCAAGCGCTGGt                                                                                                                                           <  4:396408/118‑1 (MQ=255)                                         acGAAGGCATCAGCAGCGTGCTCGACCAGCAACCACCCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGAtt                                                                                                           >  5:420979/1‑149 (MQ=255)                                         acGAAGGCATCAGCAGCGTGCTCGACCAGCAACCACCCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCAAgcgc                                                                                                                                               >  2:358781/1‑113 (MQ=255)                                         acGAAGGCATCAGCAGCGTGCTCGACCAGCAACCACCCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCAAgcgc                                                                                                                                               <  1:358781/113‑1 (MQ=255)                                          cGAAGGCATCAGCAGCGTGCTCGACCAGCAACCACCCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTg                                                                                                          >  7:1991/1‑149 (MQ=255)                                               tcATCAGCAGCTTGCTCGACAATCAACCACCCGAAATGCAACGCTTTTCGCGCAACCTGTAAATGGCGAATGCACTGAACGATTACCCTTTTCACCAGCGCTTTCGCTTGTTCACCTATGACTGGCTGCGTTATGACCAGATTGGCGAc                                                                                                     <  8:181720/148‑1 (MQ=255)                                                  tCAGCAGCGTGCTCGACCAGCAACCACCCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCa                                                                                                  <  4:372995/149‑1 (MQ=255)                                                     gcagcGTGCTCGACCAGCAACCACCCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACtg                                                                                               <  4:295141/149‑1 (MQ=255)                                                        gcGTGCTCGACCAGCAACCACCCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACtggtg                                                                                            <  6:105058/149‑1 (MQ=255)                                                         cGTGCTCGACCAGCAACCACCCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACtggtgg                                                                                           >  3:200364/1‑149 (MQ=255)                                                          gTGCTCGACCAGCAACCACCCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTggtggt                                                                                          <  8:220994/149‑1 (MQ=255)                                                            gCTCGACCAGCAACCACCCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCa                                                                                        >  8:47007/1‑149 (MQ=255)                                                             cTCGACCAGCAACCACCCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCAc                                                                                       >  5:180598/1‑149 (MQ=255)                                                                gACCAGCAACCACCCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGa                                                                                    >  3:382583/1‑149 (MQ=255)                                                                   cAGCAACCACCCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCt                                                                                 >  7:2086/1‑149 (MQ=255)                                                                     gCAACCACCCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTgc                                                                               <  2:205878/149‑1 (MQ=255)                                                                        accaccCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCa                                                                            >  3:358936/1‑149 (MQ=255)                                                                         ccaccCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCtt                                                                                                                                                   >  5:152213/1‑77 (MQ=255)                                                                         ccaccCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCtt                                                                                                                                                   <  6:152213/77‑1 (MQ=255)                                                                          caccCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATg                                                                          <  6:180598/149‑1 (MQ=255)                                                                          caccCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATg                                                                          <  8:1991/149‑1 (MQ=255)                                                                          caccCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGAACTGGTGGTCACCGACCTGCGCATg                                                                          >  8:320067/1‑149 (MQ=255)                                                                           accCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGAcc                                                                                                                <  4:248552/110‑1 (MQ=255)                                                                           accCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGAcc                                                                                                                >  3:248552/1‑110 (MQ=255)                                                                           accCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATgg                                                                         <  8:2086/149‑1 (MQ=255)                                                                            cccGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCAc                                                                                                                                       >  4:228461/1‑86 (MQ=255)                                                                            cccGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCAc                                                                                                                                       <  3:228461/86‑1 (MQ=255)                                                                              cGAAATGCAAGGCTTGTCGCGCAACCTGGATCTGGCGCAAGCACTGAAGGATTACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTTCGTTATGACCa                                                                                                               <  4:176863/108‑1 (MQ=255)                                                                              cGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCa                                                                                                               >  3:176863/1‑108 (MQ=255)                                                                                    gCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCgg                                                                >  4:243985/1‑149 (MQ=255)                                                                                           ttGTCGCGCAACCTGGAACTGGCGCAAGCACTCAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTa                                                         >  1:257736/1‑149 (MQ=255)                                                                                            tGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTac                                                        >  5:282814/1‑149 (MQ=255)                                                                                                 cgcAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCAc                                                                                       <  8:342056/113‑1 (MQ=255)                                                                                                 cgcAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCAc                                                                                       >  7:342056/1‑113 (MQ=255)                                                                                                  gcAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCttt                                                  <  4:200364/149‑1 (MQ=255)                                                                                                       cTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATc                                                                    <  1:12025/126‑1 (MQ=255)                                                                                                       cTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATc                                                                    >  2:12025/1‑126 (MQ=255)                                                                                                         ggAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTc                                            >  1:358570/1‑148 (MQ=255)                                                                                                          gAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCaa                                          <  2:358570/149‑1 (MQ=255)                                                                                                             cTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACAt                                       <  4:382583/149‑1 (MQ=255)                                                                                                                gcgcAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGc                                               <  2:345599/138‑1 (MQ=255)                                                                                                                gcgcAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCttaatcgga                                      >  1:345599/1‑140 (MQ=255)                                                                                                                 cgcAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCt                                   <  7:47007/149‑1 (MQ=255)                                                                                                                  gcAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTc                                  <  2:257736/149‑1 (MQ=255)                                                                                                                        aCTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAgcgc                             >  3:188937/1‑148 (MQ=255)                                                                                                                          tGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTaca                                                       >  8:24319/1‑120 (MQ=255)                                                                                                                                   aCCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGGTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCGGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAggg                 <  7:320067/149‑1 (MQ=255)                                                                                                                                       gTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGt             >  2:351145/1‑149 (MQ=255)                                                                                                                                            aCCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATg        <  4:358936/149‑1 (MQ=255)                                                                                                                                            aCCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATg        >  8:84474/1‑149 (MQ=255)                                                                                                                                             ccAGCGCAAGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCt                                              <  4:384539/110‑1 (MQ=255)                                                                                                                                             ccAGCGCAAGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCt                                              >  3:384539/1‑110 (MQ=255)                                                                                                                                                  gcTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGgt  <  4:188937/149‑1 (MQ=255)                                                                                                                                                   cTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAggg                 <  2:387809/133‑1 (MQ=255)                                                                                                                                                   cTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAggg                 >  1:387809/1‑133 (MQ=255)                                                                                                                                                     |                                                                                                                                                  GTCTGGCGGGTGCTGGCCAAGACCCCGAATGGCGATTACTACGAAGGCATCAGCAGCGTGCTCGACCAGCAACCACCCGAAATGCAACGCTTGTCGCGCAACCTGGAACTGGCGCAAGCACTGAACGATGACCCGTTGCACCAGCGCTTGCGCTGGTTCACCGATGACTGGCTGCGTTATGACCAGATTGGCGACGCACTGGTGGTCACCGACCTGCGCATGGGGATCCCGGGGAATTACACCTTTCGCTTCAACATGGCTCAGCGCGATCATCAAGGGCAGTGGATGGTCGGT  >  NC_002947/3591429‑3591722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑