Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,685,344 A→C 10.1% intergenic (+26/‑33) PP_5593 → / → PP_5594 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,685,3440AC10.1% 129.6 / 3.5 47intergenic (+26/‑33)PP_5593/PP_5594hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (20/22);  new base C (3/2);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 6.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCGGGCAATTTCGCCAATGACCGTGAAAAAGCCTCCGAAGCCGGTCGCAAAGGCGGCCAGTCCTCGGGCGGCAACATGCCCCACGACCGCGAGTCGGGCCACAAGGGCGGCCGTTCGTAACTGACCGAGGCGCAGTGCCGGGGCGACCTGGCACTGCAATTATCCAAGGGGGTACTCCATGCGCCTGACTCCACTGATCATCCTGATGGGTTCGCTGAGCTTGGTCGGCGGCTGTTTCGACCGCGACAATGACCACCCTGGTAAAGATTCCGACCCCAGTAAACCTTCGCTGC  >  NC_002947/3685198‑3685491
                                                                                                                                                  |                                                                                                                                                   
cccGGGCAATTTCGCCAATGACCGTGAAAAAGCCTCCGAAGCCGGTCGCAAAGGCGGCCAGTCCTCGGGCGGCAACATGCCCCACGACCGCGAGTCGGGCCACAAGGGCGGCCGTTCGTAACTGACCGAGGCGCAGTGCCGGGGCGAcc                                                                                                                                                   >  5:29038/1‑149 (MQ=255)
cccGGGCAATTTCGCCAATGACCGTGAAAAAGCCTCCGAAGCCGGTCGCAAAGGCGGCCAGTCCTCGGGCGGCAACATGCCCCACGACCGCGAGTCGGGCCACAAGGGCGGCCGTTCGTAACTGACCGAGGCGCAGTGCCGGGGCGAcc                                                                                                                                                   <  3:382830/149‑1 (MQ=255)
  cGGGCAATTTCGCCAATGACCGTGAAAAAGCCTCCGAAGCCGGTCGCAAAGGCGGCCAGTCCTCGGGCGGCAACATGCCCCACGACCGCGAGTCGGGCCACAAGGGCGGCCGTTCGTAACTGACCGAGGCGCAGTGCCGGGGCGACCTg                                                                                                                                                 <  5:188031/149‑1 (MQ=255)
     gCAATTTCGCCAATGACCGTGAAAAAGCCTCCGAAGCCGGTCGCAAAGGCGGCCAGTCCTCGGGCGGCAACATGCCCCACGACCGCGAGTCGGGCCACAAGGGCGGCCGTTCGTAACTGACCGAGGCGCAGTGCCGGGGCGACCTGGCa                                                                                                                                              >  6:115786/1‑149 (MQ=255)
       aaTTTCGCCAATGACCGTGAAAAAGCCTCCGAAGCCGGTCGCAAAGGCGGCCAGTCCTCGGGCGGCAACATGCCCCACGACCGCGAGTCGGGCCACAAGGGCGGCCGTTCGTAACTGACCGAGGCGCAGTGCCGGGGCGACCTGGCa                                                                                                                                              <  4:60852/147‑1 (MQ=255)
       aaTTTCGCCAATGACCGTGAAAAAGCCTCCGAAGCCGGTCGCAAAGGCGGCCAGTCCTCGGGCGGCAACATGCCCCACGACCGCGAGTCGGGCCACAAGGGCGGCCGTTCGTAACTGACCGAGGCGCAGTGCCGGGGCGACCTGGCa                                                                                                                                              >  3:60852/1‑147 (MQ=255)
         tttCGCCAATGACCGTGAAAAAGCCTCCGAAGCCGGTCGCAAAGGCGGCCAGTCCTCGGGCGGCAACATGCCCCACGACCGCGAGTCGGGCCACAAGGGCGGCCGTTCGTAACTGACCGAGGCGCAGTGCCGGGGCGACCTGGCACTGc                                                                                                                                          <  7:269274/149‑1 (MQ=255)
         tttCGCCAATCACCGTGAAAAAGCCACCGAAGCCGGTCGCAAAGCCGGCCAGTCCTCGGGCGCCAACATGCCCCACGACCGCTAGTCGGCCCACAAGGGCGCCAGTACCTAAATCACCGAGGCGCAGAGCCCGCCCGACCTGGCACTg                                                                                                                                           >  6:152058/1‑148 (MQ=255)
         tatCGCCAATGACCGTGAAAAAGCCTCCGAAGCCGGTCGCAAAGGCGGCCAGTCCTCGGGCGGCAACATGCCCCACGACCGCGAGTCGGGCCACAAGGGCGGCCGTTCGTAACTGACCGAGGCGCAGTGCCAGGTCGCCCCGGCACTGc                                                                                                                                          <  7:149819/147‑1 (MQ=255)
            cGCCAATGACCGTGAAAAAGCCTCCGAAGCCGGTCGCAAAGGCGGCCAGTCCTCGGGCGGCAACATGCCCCACGACCGCGAGTCGGGCCACAAGGGCGGCCGTTCGTAACTGACCGAGGTGCAGTGCCAGGTCGCCCCGGCACTGcgcc                                                                                                                                       >  8:1497/1‑146 (MQ=255)
             gCCAATGACCGTGAAAAAGCCTCCGAAGCCGGTCGCAAAGGCGGCCAGTCCTCGGGCGGCAACATGCCCCACGACCGCGAGTCGGGCCACAAGGGCGGCCGTTCGTAACTGACCGAGGTGCAGTGCCAGGTCGCCCCGGCACTGcgcc                                                                                                                                       <  7:1497/148‑4 (MQ=255)
                     ccGTGAAAAAGCCTCCGAAGCCGGTCGCAAAGGCGGCCAGTCCTCGGGCGGCAACATGCCCCACGACCGCGAGTCGGGCCACAAGGGCGGCCGTTCGTAACTGACCGAGGCGCAGTGCCGGGGCGACCTGGCACTGCAATTATCCAAgg                                                                                                                              <  1:69519/149‑1 (MQ=255)
                       gTGAAAAAGCCTCCGAAGCCGGTCGCAAAGGCGGCCAGTCCTCGGGCGGCAACATGCCCCACGACCGCGAGTCGGGCCACAAGGGCGGCCGTTCGTAACTGACCGAGGCGCAGTGCCGGGGCGCCCTGGCACTGCAATTATCCAAgggg                                                                                                                            >  3:308004/1‑149 (MQ=255)
                        tGAAAAAGCCTCCGAAGCCGGTCGCAAAGGCGGCCAGTCCTCGGGCGGCAACATGCCCCACGACCGCGAGTCGGGCCACAAGGGCGGCCGTTCGTAACTGACCGAGGCGCAGTGCCGGGGCGACCTGGCACTGCAATTATCCAAggggg                                                                                                                           <  8:203658/149‑1 (MQ=255)
                            aaaGCCTCCGAAGCCGGTCGCAAAGGCGACCAGTCCTCGGGCGGCAACAAGCCCCACGACCGCGAGACGGGCCACAACGGCGGCCGATCGTAACTGCCCGAGGCGCAGTGCCGCGGCGACCAGGCACTGCAAATATCCAAGGCGGTACt                                                                                                                       >  7:382997/1‑149 (MQ=255)
                               gCCTCCGAAGCCGGTCGCAAAGGCGGCCAGTCCTCGGGCGGCAACATGCCCCACGACCGCGAGTCGGGCCACAAGGGCGGCCGTTCGTAACTGACCGAGGCGCAGTGCCGGGGCGACCTGGCACTGCAATTATCCAAGGGGGTACTCCa                                                                                                                    <  6:395001/149‑1 (MQ=255)
                                     gAAGCCGGTCGCAAAGGCGGCCAGTCCTCGGGCGGCAACATGCCCCACGACCGCGAGTCGGGCCACAAGGGCGGCCGTTCGTAACTGACCGAGGCGCAGTGCCGGGGCGACCTGGCACTGCAATTATCCAAGGGGGTACTCCATGCGcc                                                                                                              <  2:88483/149‑1 (MQ=255)
                                         ccGGTCGCAAAGGCGGCCAGTCCTCGGGCGGCAACATGCCCCACGACCGCGAGTCGGGCCACAAGGGCCGCCGTTCGTAACTGACCGAGGCGCAGTGCCGGGGCGCCCTGGCACTGCAATTATCCAAGGGGGTACTCCATGCGCCTGAc                                                                                                          >  7:88742/1‑149 (MQ=255)
                                           ggTCGCAAAGGCGGCCAGTCCTCGGGCGGCAACATGCCCCACGACCGCGAGTCGGGCCACAAGGGCGGCCGTTCGTAACTGACCGAGGCGCAGTGCCGGGGCGACCTGGCACTGCAATTATCCAAGGGGGTACTCCATGCGCCTGACTc                                                                                                        <  4:313862/149‑1 (MQ=255)
                                              cGCAAAGGCGGCCAGTCCTCGGGCGGCAACATGCCCCACGACCGCGAGTCGGGCCACAAGGGCGGCCGTTCGTAACTGACCGAGGCGCAGTGCCGGGGCGACCTGGCACTGCAATTATCCAAGGGGGTACTCCATGCGCCTGACTCCAc                                                                                                     <  5:115786/149‑1 (MQ=255)
                                                cAAAGGCGGCCAGTCCTCGGGCGGCAACATGCCCCACGACCGCGAGTCGGGCCACAAGGGCGGCCGTTCGTAACTGACCGAGGCGCAGTGCCGGGGCGACCTGGCACTGCAATTATcc                                                                                                                                  >  2:148022/1‑118 (MQ=255)
                                                cAAAGGCGGCCAGTCCTCGGGCGGCAACATGCCCCACGACCGCGAGTCGGGCCACAAGGGCGGCCGTTCGTAACTGACCGAGGCGCAGTGCCGGGGCGACCTGGCACTGCAATTATcc                                                                                                                                  <  1:148022/118‑1 (MQ=255)
                                                cAAAGGCGGCCAGTCCTCGGGCGGCAACATGCCCCACGACCGCGAGTCGGGCCACAAGGGCGGCCGTTCGTAACTGACCGAGGCGCAGTGCCGGGGCGACCTGGCACTGCAATTATCCAAGGGGGTACTCCAt                                                                                                                   <  2:340558/133‑1 (MQ=255)
                                                cAAAGGCGGCCAGTCCTCGGGCGGCAACATGCCCCACGACCGCGAGTCGGGCCACAAGGGCGGCCGTTCGTAACTGACCGAGGCGCAGTGCCGGGGCGACCTGGCACTGCAATTATCCAAGGGGGTACTCCAt                                                                                                                   >  1:340558/1‑133 (MQ=255)
                                                 aaaGGCGGCCAGTCCTCGGGCGGCAACATGCCCCACGACCGCGAGTCGGGCCACAAGGGCGGCCGTTCGTAACTGACCGAGGCGCAGTGCCGGGGCGACCTGGCACTGCAATTATCCAAGGGGGTACTCCATGCGCCTGACTCCACTGa                                                                                                  >  1:269674/1‑149 (MQ=255)
                                                               cTCGGGCGGCAACATGCCCCACGACCGCGAGTCGGGCCACAAGGGCGGCCGTTCGTAACTGACCGAGGCGCAGTGCCGGGGCGACCTGGCACTGCAATTATCCAAGGGGGTAATCCATGAGCCTGACTCCACTGATCATCCTGATTGGt                                                                                    >  7:353670/1‑149 (MQ=255)
                                                                   ggcggcAACATGCCCGACTTCCGCGAGGCGGGGCAGAAGGGCGGCCGTTCGTAACTGTCCGTGGCGGAGTGCCGGGGGGAGCTGGGACTGCAATTATCCAAGGGGGTACTCGATGCGGCTGACTCCTCTGATGTTCCTGATGGGTTCGc                                                                                <  8:382997/149‑1 (MQ=255)
                                                                        cAACATGCCCCACGACCGCGAGTCGGGCCACAAGGGCGGCCGTTCGTAACTGACCGAGGCGCAGTGCCGGGGCGACCTGGCACTGCAATTATCCAAGGGGGTACTCCATGCGCCTGACTCCACTGATCATCCTGATGGGTTCGCTGAGc                                                                           >  3:303447/1‑149 (MQ=255)
                                                                               ccccACGACCGCGAGTCGGGCCACAAGGGCGGCCGTTCGTAACTGACCGAGGCGCAGTGCCGGGGCGACCTGGCACTGCAATTATCCAAGGGGGTACTCCATGCGCCTGACTCCAc                                                                                                     <  7:115945/116‑1 (MQ=255)
                                                                               ccccACGACCGCGAGTCGGGCCACAAGGGCGGCCGTTCGTAACTGACCGAGGCGCAGTGCCGGGGCGACCTGGCACTGCAATTATCCAAGGGGGTACTCCATGCGCCTGACTCCAc                                                                                                     >  8:115945/1‑116 (MQ=255)
                                                                                cccACGACCGCGAGTCGGGCCACAAGGGCGGCCGTTCGTAACTGACCGAGGCGCAGTGCCGGGGCGACCTGGCACTGCAATTATCCAAGGGGGTACTCCATGCGCCTGACTCCACTGATCATCCTGATGGGTTCGCTGAGCTTGGTcgg                                                                   >  6:74773/1‑149 (MQ=255)
                                                                                 ccACGACCGCGAGTCGGGCCACAAGGGCGGCCGTTCGTAACTGACCGAGGCGCAGTGCCGGGGCGACCTGGCACTGCAATTATCCAAGGGGGTACTCCATGCGCCTGACTCCACTGatcatc                                                                                             <  1:443/122‑1 (MQ=255)
                                                                                 ccACGACCGCGAGTCGGGCCACAAGGGCGGCCGTTCGTAACTGACCGAGGCGCAGTGCCGGGGCGACCTGGCACTGCAATTATCCAAGGGGGTACTCCATGCGCCTGACTCCACTGatcatc                                                                                             >  2:443/1‑122 (MQ=255)
                                                                                  cACGACCGCGAGTCGGGCCACAAGGGCGGCCGTTCGTAACTGACCGAGGCGCAGTGCCGGGGCGACCTGGCACTGCAATTATCCAAGGGGGTACTCCATGCGCCTGACTCCACTGATCATCCTGATGGGTTCGCTGAGCTTGGTcggcg                                                                 >  7:44138/1‑149 (MQ=255)
                                                                                                  gCCACAAGGGCGGCCGTTCGTAACTGACCGAGGCGAAGTGCCGGGGCGACCTGGCACTGCAATTATCCAAGGGGGTACTCGATGCGCCTGACTCCACTGATCATCCTGATGGGTTCGCTGAGCTTGGTCGGCGGCTGTTTCGACCGCGa                                                 <  8:44138/149‑1 (MQ=255)
                                                                                                    cacaAGGGCGGCCGTTCGTAACTGACCGAGGCGCAGTGCCGGGGCGACCTGGCACTGCAATTATCCAAGGGGGTACTCCATGCGCCTGACTCCACTGATCATCCTGATGGGTTCGCTGAGCTTGGTCGGCGGCTGTTTAGACCGCGAc                                                >  1:381000/1‑148 (MQ=255)
                                                                                                      caAGGGCGGCCGTTCGTAACTGACCGAGGCGCAGTGCCGGGGCGACCTGGCACTGCAATTATCCAAGGGGGTACTCCATGCGCCTGGCTCCACTGATCATCCTGATGGGTTCGCTGAGCTTGGTCGGCGGCTGTTTCGACCGCGACAAt                                             >  4:240852/1‑149 (MQ=255)
                                                                                                          ggcggcCGTTCGTTACTGACCGAGGCGCAGTGCCGGGGCGACCTGGCAGTGCAATTATCCAAGGGGGTACTCCATGCGCCTGACTCCACTGATCATCCTGATGGGTTCGCTGAGCTTGGTCGGCGGCTGTTTCGACCGCGACAATGac                                          <  8:88742/148‑1 (MQ=255)
                                                                                                                cGTTCGTAACTGACCGAGGCGCAGTGCCGGGGCGACCTGGCACTGCAATTATCCAAGGGGGTACTCCATGCGCCTGACTCCACTGATCATCCTGATGGGTTCGCTGAGCTTGGTCGGCGGCTGTTTCGACCGCGACAATGACCACCCTg                                   <  3:240852/149‑1 (MQ=255)
                                                                                                                  ttCGTAACTGACCGAGGCGCAGTGCCGGGGCGACCTGGCACTGCAATTATCCAAGGGGGTACTCCATGCGCCTGACTCCACTGATCATCCTGATGGGTTCGCTGAGCTTGGTCGGCGGCTGTTTCGACCGCGACAATGACCACCCTgg                                  >  6:130872/1‑148 (MQ=255)
                                                                                                                     gTAACTGACCGAGGCGCAGTGCCGGGGCGACCTGGCACTGCAATTATCCAAGGGGGTACTCCATGCGCCTGACTCCACTGATCATCCTGATGGGTTCGCTGAGCTTGGTCGGCGGCTGTTTCGACCGCGACAATGACCACCCTGGTaa                               <  4:303447/148‑1 (MQ=255)
                                                                                                                       aaCTGACCGAGGCGCAGTGCCGGGGCGACCTGGCACTGCAATTATCCAAGGGGGTACTCCATGCGCCTGACTCCACTGATCATCCTGa                                                                                         <  3:798/88‑1 (MQ=255)
                                                                                                                       aaCTGACCGAGGCGCAGTGCCGGGGCGACCTGGCACTGCAATTATCCAAGGGGGTACTCCATGCGCCTGACTCCACTGATCATCCTGa                                                                                         >  4:798/1‑88 (MQ=255)
                                                                                                                          tGACCGAGGCGCAGTGCCGGGGCGACCTGGCACTGCAATTATCCAAGGGGGTACTCCATGCGCCTGACTCCACTGATCATCCTGATGGGTTCGCTGAGCTTGGTCGGCGGCTGTTTCGACCGCGACAATGACCACCCTGGTAAAGATTc                         >  6:284302/1‑149 (MQ=255)
                                                                                                                             ccGAGGCGCAGTGCCGGGGCGACCTGGCACTGCAATTATCCAAGGGGGTACTCCATGCGCCTGACTCCACTGATCATCCTGATGGGTTCGCTGAGCTTGGTCGGCGGCTGTTTCGACCGCGACAATGACCACCCTGGTAAAGATTCCGa                      >  5:355183/1‑149 (MQ=255)
                                                                                                                               gAGGCGCAGTGCCGGGGCGACCTGGCACTGCAATTATCCAAGGGGGTACTCCATGCGCCTGACTCCACTGATCATCCTGATGGGTTCGCTGAGCTTGGTCGGCGGCTGTTTCGACCGCGACAATGACCACCCTGGTAAAGATTCCGAcc                    <  5:74773/149‑1 (MQ=255)
                                                                                                                                                 gACCTGGCACTGCAATTATCCAAGGGGGTACTCCATGCGCCTGACTCCACTGATCATCCTGATGGGTTCGCTGAGCTTGGTCGGCGGCTGTTTCGACCGCGACAATGACCACCCTGGTAAAGATTCCGACCCCAGTAAACCTTCGCTGc  <  2:269674/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CCCGGGCAATTTCGCCAATGACCGTGAAAAAGCCTCCGAAGCCGGTCGCAAAGGCGGCCAGTCCTCGGGCGGCAACATGCCCCACGACCGCGAGTCGGGCCACAAGGGCGGCCGTTCGTAACTGACCGAGGCGCAGTGCCGGGGCGACCTGGCACTGCAATTATCCAAGGGGGTACTCCATGCGCCTGACTCCACTGATCATCCTGATGGGTTCGCTGAGCTTGGTCGGCGGCTGTTTCGACCGCGACAATGACCACCCTGGTAAAGATTCCGACCCCAGTAAACCTTCGCTGC  >  NC_002947/3685198‑3685491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑