Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,686,436	C→T	7.7%	L282L (CTG→TTG)	PP_3251 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,686,436	0	C	T	7.7%	227.6 / 6.4	78	L282L (CTG→TTG)	PP_3251	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (39/33);  new base T (3/3);  total (42/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.58e-01

CCACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTACAGT  >  NC_002947/3686297‑3686583                                                                                                                                              |                                                                                                                                                    ccACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGc                                                                                                                                             >  5:369913/1‑148 (MQ=255)   aCGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGaa                                                                                                                                          <  4:305121/149‑1 (MQ=255)   aCGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGaa                                                                                                                                          >  3:255009/1‑149 (MQ=255)   aCGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGaa                                                                                                                                          >  7:286755/1‑149 (MQ=255)      cccgccTGGCAATTCAGGTGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGCCTTGCAGGTCGAACTg                                                                                                                                       <  8:286517/149‑1 (MQ=255)       ccgccTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGt                                                                                                                                      >  1:216328/1‑149 (MQ=255)        cgccTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGCCTTGCAGGTCGAACTgg                                                                                                                                      >  4:406548/1‑147 (MQ=255)        cgccTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGCCTTGCAGGTCGAACTgg                                                                                                                                      <  3:406548/148‑2 (MQ=255)           cTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCaa                                                                                                                                                >  8:284905/1‑135 (MQ=255)           cTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTg                                                                                                                                   >  3:244780/1‑148 (MQ=255)                aaTTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGCCTTGCAGGTCGAACTgg                                                                                                                                      <  8:333440/140‑2 (MQ=255)                aaTTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGCCTTGCAGGTCGAACTgg                                                                                                                                      >  7:333440/1‑139 (MQ=255)                aaTTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGccc                                                                                                                             >  1:100903/1‑149 (MQ=255)                aaTTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGACAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGccc                                                                                                                             >  7:113179/1‑149 (MQ=255)                    cAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTg                                                                                                                          >  4:276207/1‑148 (MQ=255)                     aGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGaa                                                                                                                                          >  7:171932/1‑131 (MQ=255)                     aGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGaa                                                                                                                                          <  8:171932/131‑1 (MQ=255)                         gcTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGtt                                                                                                                                     >  6:81843/1‑132 (MQ=255)                         gcTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGtt                                                                                                                                     <  5:81843/132‑1 (MQ=255)                          cTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCaa                                                                                                                   <  8:286755/149‑1 (MQ=255)                                  gCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTc                                                                                                            >  1:390469/1‑148 (MQ=255)                                        ccccGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGcc                                                                                                     <  1:271045/149‑1 (MQ=255)                                        ccccGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGc                                                                                                      >  7:52120/1‑148 (MQ=255)                                        ccccGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTCTTTGCAGCCCTTGAACGCAAGCGCGTCTATGGc                                                                                                      >  5:389699/1‑148 (MQ=255)                                           cgcgCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAAt                                                                                                  <  3:276207/149‑1 (MQ=255)                                               cATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCTTCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTg                                                                                                <  1:328592/149‑1 (MQ=255)                                               cATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGat                                                                                              <  6:369913/149‑1 (MQ=255)                                                aTGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGatg                                                                                             <  8:52120/149‑1 (MQ=255)                                                         aGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGAc                                                                                    >  5:228470/1‑149 (MQ=255)                                                             gTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCAtt                                                                                <  6:278556/149‑1 (MQ=255)                                                              ttCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTg                                                                               <  5:365432/149‑1 (MQ=255)                                                              ttCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑AGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTg                                                                               >  2:186178/1‑149 (MQ=255)                                                                 tACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCAtt                                                                                <  1:389072/145‑1 (MQ=255)                                                                 tACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCAtt                                                                                >  2:389072/1‑145 (MQ=255)                                                                  aCGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTgccgc                                                                           >  4:277732/1‑149 (MQ=255)                                                                    gCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCt                                                                         >  4:391765/1‑149 (MQ=255)                                                                       aGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTgca                                                                      <  4:255009/149‑1 (MQ=255)                                                                          gcgcgcTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTgcagca                                                                   <  6:228470/149‑1 (MQ=255)                                                                            gcgcTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCagcagc                                                                 <  4:37475/149‑1 (MQ=255)                                                                               cTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCa                                                              <  4:341517/149‑1 (MQ=255)                                                                                   ggCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGa                                                           <  4:244780/148‑1 (MQ=255)                                                                                   ggCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGa                                                           <  3:391765/148‑1 (MQ=255)                                                                                      tACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGatcaa                                                       >  5:191859/1‑149 (MQ=255)                                                                                        ccGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGatcaatc                                                     <  8:113179/149‑1 (MQ=255)                                                                                          gCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGAtcaatcaa                                                   <  2:390469/149‑1 (MQ=255)                                                                                          gCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTCTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGAtcaatcaa                                                   >  8:140338/1‑149 (MQ=255)                                                                                           ccTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATcaatcaat                                                  <  6:191859/149‑1 (MQ=255)                                                                                               aaTGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAAtgg                                                <  7:232436/147‑1 (MQ=255)                                                                                               aaTGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAAtgg                                                <  6:6788/147‑1 (MQ=255)                                                                                                 tGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAAtggctgg                                            <  2:100903/149‑1 (MQ=255)                                                                                                   aCCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATcaatcaat                                                  <  5:106828/141‑1 (MQ=255)                                                                                                   aCCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATcaatcaat                                                  >  6:106828/1‑141 (MQ=255)                                                                                                      ggggTATCGCGACGCCAGAGCTCATC‑‑CGGCAGTTCGACCTGCTATGCGAACTGTTTGCAGCCCTTGAACGCATGCGGGTCTATGTCCAATTGATGTATGCCGACCATTGCCGCCTgctt                                                                     <  6:386589/119‑3 (MQ=255)                                                                                                      ggggTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGTATGCCGACCATTGCCGCCTgcag                                                                     >  5:386589/1‑119 (MQ=255)                                                                                                      ggggTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGaa                                       <  2:216328/149‑1 (MQ=255)                                                                                                              gccacgccaGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGgcgc                               >  2:379397/1‑149 (MQ=255)                                                                                                                 acgccaGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCAt                            >  2:131301/1‑149 (MQ=255)                                                                                                                 acgccaGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCAt                            >  7:257789/1‑149 (MQ=255)                                                                                                                   gccaGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAAtggc                                               <  3:172707/128‑1 (MQ=255)                                                                                                                   gccaGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGc                          <  2:377880/149‑1 (MQ=255)                                                                                                                    ccaGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGcc                         >  3:77419/1‑149 (MQ=255)                                                                                                                     caGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGccc                        <  6:389699/149‑1 (MQ=255)                                                                                                                        aCCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCa                     >  4:390379/1‑149 (MQ=255)                                                                                                                            cATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCg                 >  2:345176/1‑149 (MQ=255)                                                                                                                              tC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCACCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGtt               >  5:368739/1‑149 (MQ=255)                                                                                                                                 caaacAGTTCGCCTCGCAGGTCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTg              >  4:140568/5‑149 (MQ=255)                                                                                                                                   gCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCATTTGATGCATGCCGCCCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCTTTCGTTGcc            >  6:115247/1‑149 (MQ=255)                                                                                                                                   gCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCagcagc                                                                 >  6:374059/1‑96 (MQ=255)                                                                                                                                   gCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCagcagc                                                                 <  5:374059/96‑1 (MQ=255)                                                                                                                                   gCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGcc            >  3:401756/1‑149 (MQ=255)                                                                                                                                    ccAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCg           >  8:348239/1‑149 (MQ=255)                                                                                                                                       gTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCAt        >  1:30771/1‑149 (MQ=255)                                                                                                                                        ttCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCAtt       <  3:277732/149‑1 (MQ=255)                                                                                                                                        ttCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCAt        >  3:80643/1‑148 (MQ=255)                                                                                                                                        ttCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCAt        >  3:86203/1‑148 (MQ=255)                                                                                                                                           gACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGc             <  7:45356/140‑1 (MQ=255)                                                                                                                                           gACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGc             >  8:45356/1‑140 (MQ=255)                                                                                                                                            aCCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTACAg   >  2:187764/1‑149 (MQ=255)                                                                                                                                             ccTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCACCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTACAGt  <  6:368739/149‑1 (MQ=255)                                                                                                                                              cTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTACAGt  >  5:408977/1‑148 (MQ=255)                                                                                                                                              |                                                                                                                                                    CCACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTACAGT  >  NC_002947/3686297‑3686583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑