Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,686,443	G→T	8.2%	G284V (GGC→GTC)	PP_3251 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,686,443	0	G	T	8.2%	265.0 / 10.6	85	G284V (GGC→GTC)	PP_3251	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (41/37);  new base T (3/4);  total (44/41)
Fisher's exact test for biased strand distribution p-value = 7.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.75e-01

CCACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTACAGTCTCATG  >  NC_002947/3686297‑3686589                                                                                                                                                     |                                                                                                                                                   ccACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGc                                                                                                                                                   >  5:369913/1‑148 (MQ=255)   aCGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGaa                                                                                                                                                <  4:305121/149‑1 (MQ=255)   aCGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGaa                                                                                                                                                >  3:255009/1‑149 (MQ=255)   aCGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGaa                                                                                                                                                >  7:286755/1‑149 (MQ=255)      cccgccTGGCAATTCAGGTGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGCCTTGCAGGTCGAACTg                                                                                                                                             <  8:286517/149‑1 (MQ=255)       ccgccTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGt                                                                                                                                            >  1:216328/1‑149 (MQ=255)        cgccTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGCCTTGCAGGTCGAACTgg                                                                                                                                            <  3:406548/148‑2 (MQ=255)        cgccTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGCCTTGCAGGTCGAACTgg                                                                                                                                            >  4:406548/1‑147 (MQ=255)           cTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTg                                                                                                                                         >  3:244780/1‑148 (MQ=255)                aaTTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGCCTTGCAGGTCGAACTgg                                                                                                                                            >  7:333440/1‑139 (MQ=255)                aaTTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGCCTTGCAGGTCGAACTgg                                                                                                                                            <  8:333440/140‑2 (MQ=255)                aaTTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGccc                                                                                                                                   >  1:100903/1‑149 (MQ=255)                aaTTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGACAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGccc                                                                                                                                   >  7:113179/1‑149 (MQ=255)                    cAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTg                                                                                                                                >  4:276207/1‑148 (MQ=255)                     aGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGaa                                                                                                                                                <  8:171932/131‑1 (MQ=255)                     aGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGaa                                                                                                                                                >  7:171932/1‑131 (MQ=255)                         gcTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGtt                                                                                                                                           <  5:81843/132‑1 (MQ=255)                         gcTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGtt                                                                                                                                           >  6:81843/1‑132 (MQ=255)                          cTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCaa                                                                                                                         <  8:286755/149‑1 (MQ=255)                                  gCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTc                                                                                                                  >  1:390469/1‑148 (MQ=255)                                        ccccGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGcc                                                                                                           <  1:271045/149‑1 (MQ=255)                                        ccccGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGc                                                                                                            >  7:52120/1‑148 (MQ=255)                                        ccccGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTCTTTGCAGCCCTTGAACGCAAGCGCGTCTATGGc                                                                                                            >  5:389699/1‑148 (MQ=255)                                           cgcgCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAAt                                                                                                        <  3:276207/149‑1 (MQ=255)                                               cATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCTTCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTg                                                                                                      <  1:328592/149‑1 (MQ=255)                                               cATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGat                                                                                                    <  6:369913/149‑1 (MQ=255)                                                aTGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGatg                                                                                                   <  8:52120/149‑1 (MQ=255)                                                         aGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGAc                                                                                          >  5:228470/1‑149 (MQ=255)                                                             gTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCAtt                                                                                      <  6:278556/149‑1 (MQ=255)                                                              ttCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTg                                                                                     <  5:365432/149‑1 (MQ=255)                                                              ttCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑AGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTg                                                                                     >  2:186178/1‑149 (MQ=255)                                                                 tACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCAtt                                                                                      >  2:389072/1‑145 (MQ=255)                                                                 tACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCAtt                                                                                      <  1:389072/145‑1 (MQ=255)                                                                  aCGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTgccgc                                                                                 >  4:277732/1‑149 (MQ=255)                                                                    gCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCt                                                                               >  4:391765/1‑149 (MQ=255)                                                                       aGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTgca                                                                            <  4:255009/149‑1 (MQ=255)                                                                          gcgcgcTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTgcagca                                                                         <  6:228470/149‑1 (MQ=255)                                                                            gcgcTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCagcagc                                                                       <  4:37475/149‑1 (MQ=255)                                                                               cTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCa                                                                    <  4:341517/149‑1 (MQ=255)                                                                                   ggCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGa                                                                 <  4:244780/148‑1 (MQ=255)                                                                                   ggCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGa                                                                 <  3:391765/148‑1 (MQ=255)                                                                                      tACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGatcaa                                                             >  5:191859/1‑149 (MQ=255)                                                                                        ccGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGatcaatc                                                           <  8:113179/149‑1 (MQ=255)                                                                                          gCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGAtcaatcaa                                                         <  2:390469/149‑1 (MQ=255)                                                                                          gCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTCTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGAtcaatcaa                                                         >  8:140338/1‑149 (MQ=255)                                                                                           ccTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATcaatcaat                                                        <  6:191859/149‑1 (MQ=255)                                                                                               aaTGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAAtgg                                                      <  6:6788/147‑1 (MQ=255)                                                                                               aaTGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAAtgg                                                      <  7:232436/147‑1 (MQ=255)                                                                                                 tGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAAtggctgg                                                  <  2:100903/149‑1 (MQ=255)                                                                                                   aCCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATcaatcaat                                                        >  6:106828/1‑141 (MQ=255)                                                                                                   aCCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATcaatcaat                                                        <  5:106828/141‑1 (MQ=255)                                                                                                      ggggTATCGCGACGCCAGAGCTCATC‑‑CGGCAGTTCGACCTGCTATGCGAACTGTTTGCAGCCCTTGAACGCATGCGGGTCTATGTCCAATTGATGTATGCCGACCATTGCCGCCTgctt                                                                           <  6:386589/119‑3 (MQ=255)                                                                                                      ggggTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGTATGCCGACCATTGCCGCCTgcag                                                                           >  5:386589/1‑119 (MQ=255)                                                                                                      ggggTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGaa                                             <  2:216328/149‑1 (MQ=255)                                                                                                              gccacgccaGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGgcgc                                     >  2:379397/1‑149 (MQ=255)                                                                                                                 acgccaGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCAt                                  >  2:131301/1‑149 (MQ=255)                                                                                                                 acgccaGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCAt                                  >  7:257789/1‑149 (MQ=255)                                                                                                                   gccaGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAAtggc                                                     <  3:172707/128‑1 (MQ=255)                                                                                                                   gccaGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGc                                <  2:377880/149‑1 (MQ=255)                                                                                                                    ccaGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGcc                               >  3:77419/1‑149 (MQ=255)                                                                                                                     caGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGccc                              <  6:389699/149‑1 (MQ=255)                                                                                                                        aCCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCa                           >  4:390379/1‑149 (MQ=255)                                                                                                                            cATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCg                       >  2:345176/1‑149 (MQ=255)                                                                                                                              tC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCACCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGtt                     >  5:368739/1‑149 (MQ=255)                                                                                                                                 caaacAGTTCGCCTCGCAGGTCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTg                    >  4:140568/5‑149 (MQ=255)                                                                                                                                   gCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCATTTGATGCATGCCGCCCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCTTTCGTTGcc                  >  6:115247/1‑149 (MQ=255)                                                                                                                                   gCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCagcagc                                                                       >  6:374059/1‑96 (MQ=255)                                                                                                                                   gCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCagcagc                                                                       <  5:374059/96‑1 (MQ=255)                                                                                                                                   gCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGcc                  >  3:401756/1‑149 (MQ=255)                                                                                                                                    ccAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCg                 >  8:348239/1‑149 (MQ=255)                                                                                                                                       gTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCAt              >  1:30771/1‑149 (MQ=255)                                                                                                                                        ttCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCAtt             <  3:277732/149‑1 (MQ=255)                                                                                                                                        ttCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCAt              >  3:86203/1‑148 (MQ=255)                                                                                                                                        ttCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCAt              >  3:80643/1‑148 (MQ=255)                                                                                                                                           gACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGc                   <  7:45356/140‑1 (MQ=255)                                                                                                                                           gACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGc                   >  8:45356/1‑140 (MQ=255)                                                                                                                                            aCCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTACAg         >  2:187764/1‑149 (MQ=255)                                                                                                                                             ccTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCACCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTACAGt        <  6:368739/149‑1 (MQ=255)                                                                                                                                              cTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTACAGt        >  5:408977/1‑148 (MQ=255)                                                                                                                                                gCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTACAGtct      >  2:360785/1‑148 (MQ=255)                                                                                                                                                gCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTACAGtct      >  2:104878/1‑148 (MQ=255)                                                                                                                                                 cAAGTCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTACAGTCTCa    <  4:86203/149‑1 (MQ=255)                                                                                                                                                 cAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTACAGTCTCa    <  4:80643/149‑1 (MQ=255)                                                                                                                                                  aaGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACTATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCg                             <  7:261016/123‑1 (MQ=255)                                                                                                                                                   aGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTACAGTCTCATg  <  6:408977/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   CCACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATC‑‑CGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTACAGTCTCATG  >  NC_002947/3686297‑3686589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑